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Push-out bond strength and intratubular biomineralization of a hydraulic root-end filling material premixed with dimethyl sulfoxide as a vehicle
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Ju-Ha Park, Hee-Jin Kim, Kwang-Won Lee, Mi-Kyung Yu, Kyung-San Min
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Restor Dent Endod 2023;48(1):e8. Published online January 20, 2023
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DOI: https://doi.org/10.5395/rde.2023.48.e8
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Abstract
PDFPubReaderePub
- Objectives
This study was designed to evaluate the parameters of bonding performance to root dentin, including push-out bond strength and dentinal tubular biomineralization, of a hydraulic bioceramic root-end filling material premixed with dimethyl sulfoxide (Endocem MTA Premixed) in comparison to a conventional powder-liquid–type cement (ProRoot MTA). Materials and MethodsThe root canal of a single-rooted premolar was filled with either ProRoot MTA or Endocem MTA Premixed (n = 15). A slice of dentin was obtained from each root. Using the sliced specimen, the push-out bond strength was measured, and the failure pattern was observed under a stereomicroscope. The apical segment was divided into halves; the split surface was observed under a scanning electron microscope, and intratubular biomineralization was examined by observing the precipitates formed in the dentinal tubule. Then, the chemical characteristics of the precipitates were evaluated with energy-dispersive X-ray spectroscopic (EDS) analysis. The data were analyzed using the Student’s t-test followed by the Mann-Whitney U test (p < 0.05). ResultsNo significant difference was found between the 2 tested groups in push-out bond strength, and cohesive failure was the predominant failure type. In both groups, flake-shaped precipitates were observed along dentinal tubules. The EDS analysis indicated that the mass percentage of calcium and phosphorus in the precipitate was similar to that found in hydroxyapatite. ConclusionsRegarding bonding to root dentin, Endocem MTA Premixed may have potential for use as an acceptable root-end filling material.
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Change of phase transformation and bond strength of Y-TZP with various hydrofluoric acid etching
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Mi-Kyung Yu, Eun-Jin Oh, Myung-Jin Lim, Kwang-Won Lee
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Restor Dent Endod 2021;46(4):e54. Published online October 20, 2021
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DOI: https://doi.org/10.5395/rde.2021.46.e54
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Abstract
PDFPubReaderePub
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The purpose of this study was to quantify phase transformation after hydrofluoric acid (HF) etching at various concentrations on the surface of yttria-stabilized tetragonal zirconia polycrystal (Y-TZP), and to evaluate changes in bonding strength before and after thermal cycling. Materials and MethodsA group whose Y-TZP surface was treated with tribochemical silica abrasion (TS) was used as the control. Y-TZP specimens from each experimental group were etched with 5%, 10%, 20%, and 40% HF solutions at room temperature for 10 minutes. First, to quantify the phase transformation, Y-TZP specimens (n = 5) treated with TS, 5%, 10%, 20% and 40% HF solutions were subjected to X-ray diffraction. Second, to evaluate the change in bond strength before and after thermal cycling, zirconia primer and MDP-containing resin cement were sequentially applied to the Y-TZP specimen. After 5,000 thermal cycles for half of the Y-TZP specimens, shear bond strength was measured for all experimental groups (n = 10). ResultsThe monoclinic phase content in the 40% HF-treated group was higher than that of the 5%, 10%, and 20% HF-treated groups, but lower than that of TS-treated group (p < 0.05). The 40% HF-treated group showed significantly higher bonding strength than the TS, 5%, and 10% HF-treated groups, even after thermal cycling (p < 0.05). ConclusionsThrough this experiment, the group treated with SiO2 containing air-borne abrasion on the Y-TZP surface showed higher phase transformation and higher reduction in bonding strength after thermal cycling compared to the group treated with high concentration HF.
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Effect of hydrofluoric acid-based etchant at an elevated temperature on the bond strength and surface topography of Y-TZP ceramics
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Mi-Kyung Yu, Myung-Jin Lim, Noo-Ri Na, Kwang-Won Lee
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Restor Dent Endod 2020;45(1):e6. Published online December 3, 2019
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DOI: https://doi.org/10.5395/rde.2020.45.e6
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Abstract
PDFPubReaderePub
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This study investigated the effects of a hydrofluoric acid (HA; solution of hydrogen fluoride [HF] in water)-based smart etching (SE) solution at an elevated temperature on yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) ceramics in terms of bond strength and morphological changes. Materials and MethodsEighty sintered Y-TZP specimens were prepared for shear bond strength (SBS) testing. The bonding surface of the Y-TZP specimens was treated with 37% phosphoric acid etching at 20°C–25°C, 4% HA etching at 20°C–25°C, or HA-based SE at 70°C–80°C. In all groups, zirconia primers were applied to the bonding surface of Y-TZP. For each group, 2 types of resin cement (with or without methacryloyloxydecyl dihydrogen phosphate [MDP]) were used. SBS testing was performed. Topographic changes of the etched Y-TZP surface were analyzed using scanning electron microscopy and atomic force microscopy. The results were analyzed and compared using 2-way analysis of variance. ResultsRegardless of the type of resin cement, the highest bond strength was measured in the SE group, with significant differences compared to the other groups (p < 0.05). In all groups, MDP-containing resin cement yielded significantly higher bond strength values than MDP-free resin cement (p < 0.05). It was also shown that the Y-TZP surface was etched by the SE solution, causing a large change in the surface topography. ConclusionsBond strength significantly improved when a heated HA-based SE solution was applied to the Y-TZP surface, and the etched Y-TZP surface was more irregular and had higher surface roughness.
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The effect of continuous application of MDP-containing primer and luting resin cement on bond strength to tribochemical silica-coated Y-TZP
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Myung-Jin Lim, Mi-Kyung Yu, Kwang-Won Lee
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Restor Dent Endod 2018;43(2):e19. Published online April 3, 2018
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DOI: https://doi.org/10.5395/rde.2018.43.e19
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Abstract
PDFPubReaderePub
- Objectives
This study investigated the effect of continuous application of 10-methacryloyloxydecyldihydrogen phosphate (MDP)-containing primer and luting resin cement on bond strength to tribochemical silica-coated yttria-stabilized tetragonal zirconia polycrystal (Y-TZP). Materials and MethodsForty bovine teeth and Y-TZP specimens were prepared. The dentin specimens were embedded in molds, with one side of the dentin exposed for cementation with the zirconia specimen. The Y-TZP specimen was prepared in the form of a cylinder with a diameter of 3 mm and a height of 10 mm. The bonding surface of the Y-TZP specimen was sandblasted with silica-coated aluminium oxide particles. The forty tribochemical silica-coated Y-TZP specimens were cemented to the bovine dentin (4 groups; n = 10) with either an MDP-free primer or an MDP-containing primer and either an MDP-free resin cement or an MDP-containing resin cement. After a shear bond strength (SBS) test, the data were analyzed using 1-way analysis of variance and the Tukey test (α = 0.05). ResultsThe group with MDP-free primer and resin cement showed significantly lower SBS values than the MDP-containing groups (p < 0.05). Among the MDP-containing groups, the group with MDP-containing primer and resin cement showed significantly higher SBS values than the other groups (p < 0.05). ConclusionsThe combination of MDP-containing primer and luting cement following tribochemical silica coating to Y-TZP was the best choice among the alternatives tested in this study.
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Removal efficacy and cytotoxicity of a calcium hydroxide paste using N-2-methyl-pyrrolidone as a vehicle
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Myung-Jin Lim, Hyun-Jin Jang, Mi-Kyung Yu, Kwang-Won Lee, Kyung-San Min
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Restor Dent Endod 2017;42(4):290-300. Published online October 20, 2017
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DOI: https://doi.org/10.5395/rde.2017.42.4.290
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Abstract
PDFPubReaderePub
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This study investigated the removal efficacy and cytotoxicity of a newly developed calcium hydroxide paste (cleaniCal, Maruchi) using N-2-methyl-pyrrolidone (NMP) as a vehicle in comparison with ApexCal (Ivoclar Vivadent) and Calcipex II (Nishika), which use different vehicles such as polyethylene glycol and propylene glycol, respectively. Materials and MethodsThirty maxillary premolars with oval-shaped canals were divided into 3 groups and the teeth were filled with one of the pastes. After removal of the paste, micro-computed tomographic (μ-CT) imaging was obtained to assess the volume of residual paste in the root canal of each tooth. The teeth were then split longitudinally and the area of the paste-coated surface was evaluated by stereomicroscopy. The cytotoxicity of each product was assessed using an agar overlay assay. The effect of each vehicle on cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The data were analyzed using one-way analysis of variance and Tukey's tests to detect any significance (p < 0.05). ResultsIn the μ-CT and stereomicroscopic analysis, cleaniCal exhibited less remnants of medicament than ApexCal and Calcipex. cleaniCal showed a higher cytotoxicity than the other pastes in the agar overlay assay. Furthermore, NMP exhibited lower cell viability compared to the other vehicles. ConclusionscleaniCal showed better removal efficacy compared to the other products. However, clinicians should be aware of the higher cytotoxicity of the NMP-based material and consider its possible adverse effects on periradicular tissue when it is overfilled.
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Recognition and management of palatogingival groove for tooth survival: a literature review
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Hee-Jin Kim, Yoorina Choi, Mi-Kyung Yu, Kwang-Won Lee, Kyung-San Min
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Restor Dent Endod 2017;42(2):77-86. Published online April 12, 2017
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DOI: https://doi.org/10.5395/rde.2017.42.2.77
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Abstract
PDFPubReaderePub
Palatogingival groove (PGG) is an anomaly in the maxillary anterior teeth, often accompanied by the area of bony destruction adjacent to the teeth with no carious or traumatic history. The hidden trap in the tooth can harbor plaque and bacteria, resulting in periodontal destruction with or without pulpal pathologic change. Related diseases can involve periodontal destruction, combined endodontic-periodontal lesions, or separate endodontic and periodontal lesions. Disease severity and prognosis related to PGG depend on several factors, including location, range, depth, and type of the groove. Several materials have been used and recommended for cases of extensive periodontal destruction from PGG to remove and block the inflammatory source and recover the health of surrounding periodontal tissues. Even in cases of severe periodontal destruction, several studies have reported favorable treatment outcomes with proper management. With new options in diagnosis and treatment, clinicians need a detailed understanding of the characteristics, treatment, and prognosis of PGG to successfully manage the condition.
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In vitro evaluation of a newly produced resin-based endodontic sealer
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Yoo-Seok Song, Yoorina Choi, Myung-Jin Lim, Mi-Kyung Yu, Chan-Ui Hong, Kwang-Won Lee, Kyung-San Min
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Restor Dent Endod 2016;41(3):189-195. Published online July 26, 2016
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DOI: https://doi.org/10.5395/rde.2016.41.3.189
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Abstract
PDFPubReaderePub
- Objectives
A variety of root canal sealers were recently launched to the market. This study evaluated physicochemical properties, biocompatibility, and sealing ability of a newly launched resin-based sealer (Dia-Proseal, Diadent) compared to the existing root canal sealers (AHplus, Dentsply DeTrey and ADseal, Metabiomed). Materials and MethodsThe physicochemical properties of the tested sealers including pH, solubility, dimensional change, and radiopacity were evaluated. Biocompatibility was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. For microleakage test, single-rooted teeth were instrumented, and obturated with gutta-percha and one of the sealers (n = 10). After immersion in 1% methylene blue solution for 2 weeks, the specimens were split longitudinally. Then, the maximum length of staining was measured. Statistical analysis was performed by one-way analysis of variance followed by Tukey test (p = 0.05). ResultsDia-Proseal showed the highest pH value among the tested sealers (p < 0.05). ADseal showed higher dimensional change compared to AHplus and Dia-Proseal (p < 0.05). The solubility values of AHplus and Dia-Proseal were similar, whereas ADseal had the lowest solubility value (p < 0.05). The flow values of sealer in increasing order were AHplus, DiaProseal, and ADseal (p < 0.05). The radiopacity of AHplus was higher than those of ADseal and Dia-Proseal (p < 0.05). The cell viability of the tested materials was statistically similar throughout the experimental period. There were no significant differences in microleakage values among the tested samples. ConclusionsThe present study indicates that Dia-Proseal has acceptable physicochemical properties, biocompatibility, and sealing ability.
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Washout resistance of fast-setting pozzolan cement under various root canal irrigants
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Ga-Yeon Jang, Su-Jung Park, Seok-Mo Heo, Mi-Kyung Yu, Kwang-Won Lee, Kyung-San Min
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Restor Dent Endod 2013;38(4):248-252. Published online November 12, 2013
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DOI: https://doi.org/10.5395/rde.2013.38.4.248
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Abstract
PDFPubReaderePub
- Objectives
Fast-setting pozzolan cement (Endocem, Maruchi) was recently developed. The aim of this study was to investigate the effects of various root canal irrigants on the washout of Endocem in comparison to the previously marketed mineral trioxide aggregate (ProRoot; Dentsply) in a furcal perforation model. Materials and MethodsProRoot and Endocem were placed into acrylic molds on moist Oasis. Each mold was then immediately exposed to either physiologic saline, 2.5% sodium hypochlorite (NaOCl), or 2% chlorhexidine (CHX) under gentle shaking for five minutes. Washout testing was performed by scoring scanning electron microscope (SEM) images. ResultsEndocem exhibited higher washout resistance compared to ProRoot, especially in the NaOCl group. ConclusionsThese results suggest that Endocem can be considered a useful repair material for furcal perforation, especially in a single-visit scenario.
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Effect of adhesive hydrophobicity on microtensile bond strength of low-shrinkage silorane resin to dentin
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So-Yeun Cho, Hyun-Young Kang, Kyoung-A Kim, Mi-Kyung Yu, Kwang-Won Lee
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J Korean Acad Conserv Dent 2011;36(4):280-289. Published online July 31, 2011
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DOI: https://doi.org/10.5395/JKACD.2011.36.4.280
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Abstract
PDFPubReaderePub
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Objectives
The purpose of this study was to evaluate µTBS (microtensile bond strength) of current dentin bonding adhesives which have different hydrophobicity with low-shrinkage silorane resin.
Materials and Methods
Thirty-six human third molars were used. Middle dentin was exposed. The teeth were randomly assigned to nine experimental groups: Silorane self-etch adhesives (SS), SS + phosphoric acid etching (SS + pa), Adper easy bond (AE), AE + Silorane system bonding (AE + SSb), Clearfil SE bond (CSE), CSE + SSb, All-Bond 2 (AB2), AB2 + SSb, All-Bond 3 (AB3). After adhesive's were applied, the clinical crowns were restored with Filtek LS (3M ESPE). The 0.8 mm × 0.8 mm sticks were submitted to a tensile load using a Micro Tensile Tester (Bisco Inc.). Water sorption was measured to estimate hydrophobicity adhesives.
Results
µTBS of silorane resin to 5 adhesives: SS, 23.2 MPa; CSE, 19.4 MPa; AB3, 30.3 MPa; AB2 and AE, no bond. Additional layering of SSb: CSE + SSb, 26.2 MPa; AB2 + SSb, 33.9 MPa; AE + SSb, no bond. High value of µTBS was related to cohesive failure. SS showed the lowest water sorption. AE showed the highest solubility.
Conclusions
The hydrophobicity of adhesive increased, and silorane resin bond-strength was also increased. Additional hydrophobic adhesive layer did not increase the bond-strength to silorane resin except AB2 + SSb. All-Bond 3 showed similar µTBS & water sorption with SS. By these facts, we could reach a conclusion that All-Bond 3 is a competitive adhesive which can replace the Silorane adhesive system.
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Effect of moisture on sealing ability of root canal filling with different types of sealer through the glucose penetration model
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Jin-Ah Jang, Hee-Lyang Kim, Mi-Ja Her, Kwang-Won Lee, Mi-Kyung Yu
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J Korean Acad Conserv Dent 2010;35(5):335-343. Published online September 30, 2010
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DOI: https://doi.org/10.5395/JKACD.2010.35.5.335
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Abstract
PDFPubReaderePub
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Objectives
To compared the effect of different levels of moisture of root canal on the sealing ability after filling with four different types of sealer.
Materials and Methods
Single-rooted teeth (n = 90) instrumented to and apical size of 0.06 / 45 were randomly assigned to 12 experimental groups (n = 7 per group), positive/negative control groups (n = 3 per group). The teeth of the experimental groups (a. DRY; b. PAPER POINT DRY; c. WET) were obturated with sealer (Group 1-3: Sealapex; Group 4-6: AH plus; Group 7-9: Tubuli-seal; Group 10-12: EndoRez) and warm vertical compaction method. After 7 days in 37℃, 100% humidity, the coronal-to-apical microleakage was evaluated quantitatively using a glucose leakage model. The leaked glucose concentration was measured with spectrophotometer at 1, 3, 7, 14, 21, and 30 days. Data were recorded ad mmol/L and statistically analysed with the two-way ANOVA and Duncan test (p = 0.05).
Results
Throughout the experimental period Tubuli-seal/WET (Group 9) showed the highest mean cumulative glucose penetration (178.75 mmol/L), whereas AH plus/DRY (Group 4) had the least (20.78 mmol/L).
Conclusions
The results of this study demonstrated that the moisture condition of root canals at the time of obturation and the type of sealer that was used had a significant effect on leakage and sealing ability. Thus drying procedure according to sealer types is a critical step and should not be missed in endodontic treatment.
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Effect of soft chelating irrigation on the sealing ability of GP/AH Plus root fillings
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Yi-Suk Yu, Tae-Gun Kim, Kwang-Won Lee, Mi-Kyung Yu
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J Korean Acad Conserv Dent 2009;34(6):484-490. Published online November 30, 2009
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DOI: https://doi.org/10.5395/JKACD.2009.34.6.484
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Abstract
PDFPubReaderePub
The purpose of this study was to evaluate the effect of soft chelating irrigant on the sealing ability of root fillings by using a glucose leakage test.
A total of 45 single-rooted teeth were selected for the study. The teeth were decoronated leaving a total length of 13mm. The root canals prepared using K3 NiTi rotary instruments to an apical dimension of size 45(0.06 taper). The specimens were then randomly divided into 3 experimental groups of 13 roots each and 2 control groups of 3 roots each. Specimen in each group were prepared with different irrigation protocols : group 1, 2.5% NaOCl; group 2, 2.5% NaOCl and 17% EDTA; group 3, 2.5% NaOCl and 15% HEBP. The root canals were filled with gutta-percha and AH Plus sealer using lateral condensation. After 7 days in 37℃, 100% humidity, the coronal-to-apical microleakage was evaluated quantitatively using a glucose leakage model. The leaked glucose concentration was measured with spectrophotometry at 1, 4, 7, 14, 21 and 28 days.
There was a tendency of increase in leakage in all experimental groups during experimental period. HEBP-treated dentin showed no significant difference with EDTA-treated dentin during experimental period. From the 21th day onward, HEBP-treated dentin showed significantly lower leakage than smear-covered dentin. HEBP-treated dentin displayed a similar sealing pattern to EDTA-treated dentin and a better sealing ability than smear-covered dentin. Consequently, a soft chelator(HEBP) could be considered as the possible alternative to EDTA.
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Changes in µ-TBS to pulp chamber dentin after the application of NaOCl & reversal effect by using sodium ascorbate
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Su-Mi Kwon, Tae-Gun Kim, Mi-Kyung Yu, Kwang-Won Lee
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J Korean Acad Conserv Dent 2009;34(6):515-525. Published online November 30, 2009
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DOI: https://doi.org/10.5395/JKACD.2009.34.6.515
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Abstract
PDFPubReaderePub
Clinical suggestion for the limitation of application time of NaOCl solution is needed to avoid large reductions in resin-dentin bond strength. The aim of this study was to measure the change of µ-tensile bond strength after the various application time of 5.25% NaOCl solution to pulp chamber dentin in endodontic access cavity, and to evaluate the effect of 10% sodium ascorbate application for 10 min on bond strength after the treatment of 5.25% NaOCl solution. In this experiment, there were no statistical differences(p>0.05) in bond strengths between upper chamber dentin and lower chamber dentin. NaOCl-treated group for 20 min did not show any significant decrease(p>0.05) in bond strength than non-treated control group. In contrast to that, bond strengths of NaOCl-treated groups for 40 & 80 min were significantly lower(p<0.05) than that of non-treated control group.
10% sodium ascorbate retreated group for 10 min after 5.25% NaOCl application for 40 min to chamber dentin showed the recovery of bond strength significantly. However, the bond strength of sodium ascorbate retreated group after 5.25% NaOCl application for 80 min was still significantly lower(p<0.05) compared to the non-treated control group, which means the reductions in resin-dentin bond strength were not fully reversed. On the contrary, sodium ascorbate retreated group after 5.25% NaOCl application for 5 min showed significantly higher(p<0.05) bond strength compared to the control group, which demonstrates its superior recovery effect. In SEM exminations of specimens retreated with 10% sodium ascorbate after NaOCl application for 40 & 80 min showed that resin tags were formed clearly and densely, but weakly in density and homogeneity of individual resin tag compared to the control specimen.
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Influence of Sodium Ascorbate on Microtensile Bond Strengths to Pulp Chamber Dentin treated with NaOCl
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Soo-Yeon Jeon, Kwang-Won Lee, Mi-Kyung Yu
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J Korean Acad Conserv Dent 2008;33(6):545-552. Published online November 30, 2008
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DOI: https://doi.org/10.5395/JKACD.2008.33.6.545
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Abstract
PDFPubReaderePub
The purpose of this study was to evaluate the influence of sodium ascorbate on microtensile bond strengths of total-etching adhesive system to pulp chamber dentin treated with NaOCl.
Pulp chambers of extracted human non-caries permanent molars were treated as follows: group 1, with 0.9% NaCl; group 2, with 5.25% NaOCl; group 3, with 5.25% NaOCl and 10% sodium ascorbate for 1min; group 4, with 5.25% NaOCl and 10% sodium ascorbate for 1 min and 10ml of water; group 5, with 5.25% NaOCl and 10% sodium ascorbate for 5 min; group 6, with 5.25% NaOCl and 10% sodium ascorbate for 5 min and 10ml of water; group 7, with 5.25% NaOCl and 10% sodium ascorbate for 10 min; group 8, with 5.25% NaOCl and 10% sodium ascorbate for 10 min and 10ml of water. Treated specimens were dried, bonded with a total-etching adhesive system (Single bond), restored with a composite resin(Z250) and kept for 24h at 100% humidity to measure the microtensile bond strength.
NaOCl-treated group (group 2) demonstrated significantly lower strength than the other groups. No significant difference in microtensile bond strengths was found between NaCl-treated group (group 1) and sodium ascorbate-treated groups (group 3-8). The results of this study indicated that dentin treated with NaOCl reduced the microtensile bond strength of Single bond. Application of 10% sodium ascorbate restored the bond strength of Single bond on NaOCl-treated dentin. Application time of sodium ascorbate did not have a significant effect.
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The Effect of Temporary Filling Materials on The Adhesion between Dentin Adhesive-coated Surface and Resin Inlay
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Tae-Gun Kim, Kwang-Won Lee, Mi-Kyung Yu
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J Korean Acad Conserv Dent 2008;33(6):553-559. Published online November 30, 2008
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DOI: https://doi.org/10.5395/JKACD.2008.33.6.553
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Abstract
PDFPubReaderePub
The purpose of this research was to compare the microtensile bond strength of resin coated surface and resin inlay according temporary filling materials prior to applying self-adhesive resin cement. Caviton(GC, Japan), Provifil(Promedica, Neumunster, Germany), Provifil(Promedica, Neumunster, Germany) & petrolatum, and Eugenol-based cement, Tembond(Kerr, Orange CA, USA) were used as temporary filling materials. After fabrication of Tescera(Bisco, Schamburg IL, USA), it was bonded with a self-adhesive resin cement, Rely X unicem(3M, St. Paul. Minn, USA). After this procedure, the microtensile bond strength was measured and it was analyzed through one-way ANOVA and Duncan test(p<0.05).
Caviton(GC, Tokyo, Japan) showed statistical difference except for the control(group I) and the saliva(group II)(p<0.05). Provifil(group IV), Provifil & petroneum(group V), Tembond(group VI) had lower microtensile bond strength.
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THE CHANGE OF ADAPTABILITY CHANGE IN ADHESIVE SYSTEMS TO DENTIN SUBSTRTE ACCORDING TO STORAGE TIME
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Young-Gon Cho, Il-Hwan Ban, Mi-Kyung Yu
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J Korean Acad Conserv Dent 2005;30(3):204-214. Published online January 14, 2005
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DOI: https://doi.org/10.5395/JKACD.2005.30.3.204
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Abstract
PDFPubReaderePub
- ABSTRACT
This study compared the microtensile bond strength (μTBS) and microscopic change of two 2-step and two 1-step self-etching adhesives to dentin according to storage times in distilled water.
Occlusal dentin was exposed in 48 human molars. They were divided to four groups by different adhesives: SE Bond group (Clearfil SE Bond), AdheSE group (AdheSE), Adper group (Adper Prompt L-Pop), and Xeno group (Xeno III). Each group was stored in 37℃ distilled water for 1, 15, and 30 days.
Resin-bonded specimens were sectioned into beams and subjected to μTBS testing with a crosshead speed of 1 mm/minute. For SEM observation, one specimen was selected and sectioned in each group after each stroage time. Resin-dentin interface was observed under FE-SEM.
In all storage times, mean μTBS of SE group was significantly higher than those of other groups (p < 0.05). There was no significant difference between mean μTBS of SE group and AdheSE group among all storage times, but significant difference between 1- and 30-day storage in mean μTBS of Adper group and Xeno group (p < 0.05). For 1-and 15-day storage, all groups showed the close adaptation between resin-dentin interfaces. For 30-day storage, resin-dentin interfaces showed wide gap in Adper group and separate pattern in Xeno III group.
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Effect of canal tapering in teeth of various apical size & cross-sectional configuration on microleakage
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Jung-Hee Kim, Kyung-Ha Lee, Se-Joon Lee, Mi-Kyung Yu, Kwang-Won Lee
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J Korean Acad Conserv Dent 2005;30(2):95-101. Published online March 31, 2005
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DOI: https://doi.org/10.5395/JKACD.2005.30.2.095
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Abstract
PDFPubReaderePub
The aim of this study was to evaluate the microleakage of teeth according to root canal preparation with & without apical enlargement in various size of apical foramen. 60 extracted one canal roots were cross-cutted at 5 mm from root apex and divided into two groups according to their apical foramen size of large (L) and small (S). Each group was subdivided into two groups accordance with their cross-sectional configuration at 5 mm from apex, round (R) and ovoid (O); SR Group, SO Group, LR Group, LO Group. Each group was shaped in .02 taper by Quantec series Nickel-Titanium (NiTi) rotary file, obturated by lateral condensation method. Leakage was measured using a fluid transport model under 40 cmH2O pressure. After the leakage test, blocks which had showed the leakage retreated with .04 taper and .06 taper and evaluated the degree of fluid filtration in each group. The data was analysed statistically using chi-square test and fisher's exact test.
The results obtained were as follows:
1. Significant difference in leakage was found in groups which had different apical foramen size in .02 taper instrumentation (p < 0.05), but not in .04 taper instrumentation (p > 0.05).
2. The difference in microleakage according to the shape of canal was not evident at 5 mm from apex (p > 0.05).
3. There was correlation between .02 taper instrumentation and .04 taper instrumentation in LR group , LO group (p < 0.05).
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Surface roughness and microleakage of class V composite restorations : Effect of surface sealing
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Min-Jeong Kim, Mi-Jeong Lee, Mi-Kyung Yu, Soo-Joung Park, Kwang-Won Lee
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J Korean Acad Conserv Dent 2005;30(1):22-30. Published online January 31, 2005
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DOI: https://doi.org/10.5395/JKACD.2005.30.1.022
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Abstract
PDFPubReaderePub
The purpose of this study was to compare the effect of surface sealing materials on microleakage and surface roughness in Class V composite restorations.
Twenty five standardized Class V cavity preparations were made on the facial surface of human premolars and were randomly assigned to 5 groups. The teeth were restored with Z-250 after applying Single Bond. Following 7 days storage in distilled water at 37℃, the restorations were sealed as following systems : No sealing ; Single Bond Adhesive ; Biscover ; Fortify ; Optiguard. Then, toothbrush abrasion test was conducted using a wear testing machine.
Surface roughness was measured by means of profilometer before and after toothbrushing and the results were statistically analysed by using a paired t-test and ANOVA. The bonded interfaces and the changes of surface roughness were examined by SEM.
For microleakage test, specimens were stained in a 2% methylene blue solution, then longitudinally sectioned and analyzed for leakage at occlusal and cervical interfaces using stereomicroscope. The results were statistically analysed by using a Kruskal-Wallis and Mann-Whitney U test.
Surface roughness was increasing in all groups after toothbrushing, but no statistically significant differences. In SEM observation, surface sealant was partially retained and partially detached in bonded interfaces. Especially, microgap was identified in cervical margins. In microleakage test, there was better seal in the enamel region and a significant difference between groups at occlusal margin. Control group and Single Bond group had significantly better marginal seal at enamel margin than cervical margin.
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An in-vitro evaluation of sealer placement methods in simulated root canal extensions
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Sung-Young Kim, Mi-Jeong Lee, Jang-Won Moon, Se-Joon Lee, Mi-Kyung Yu
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J Korean Acad Conserv Dent 2005;30(1):31-37. Published online January 31, 2005
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DOI: https://doi.org/10.5395/JKACD.2005.30.1.031
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Abstract
PDFPubReaderePub
The aim of this study was to evaluate the effectiveness of sealer placement in simulated root canal extensions. Forty resin blocks were attained from the Endo-training Bloc. In each block, the simulated root canal was made with #20, 08taper GT file. After each block was longitudinally split into two halves, a standardized groove was prepared on one canal wall of two halves to simulate the canal extensions with various irregularities. The two halves of each block were assembled and all simulated root canals were obturated by single cone method with AH26 sealer. Four different methods of sealer placement were used: group A, #20 K-file; group B, ultrasonic file; group C, lentulo spiral; group D, EZ-Fill bi-directional spiral. All obturated blocks were stored in 100% humidity at 37℃ for 1 week. Using a low speed saw, each block was sectioned horizontally. Images of the sections were taken using a stereomicroscope at × 30 magnification and a digital camera. The amount of the sealer in the groove was evaluated using a scoring system, a higher score indicated better sealing effectiveness. The data was statistically analysed by Fisher's Exact Test.
The sealing score was the lowest, specially at the middle area of canal extensions in group A, and that was statistically significant difference from other groups. In conclusion, the ultrasonic file, lentulo spiral and EZ-Fill bi-directional spiral were effective methods of sealer placement in simulated canal extensions. The K file was the least effective method, specially at the middle area of canal extensions.
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Effect of a desensitizer on microtensile bond strength of different adhesives
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Sung-Yeon Hwang, Kyung-Ha Lee, Mi-Kyung Yu, Kwang-Won Lee
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J Korean Acad Conserv Dent 2003;28(5):378-384. Published online September 30, 2003
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DOI: https://doi.org/10.5395/JKACD.2003.28.5.378
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Abstract
PDFPubReaderePub
This study evaluated the influence of a desensitizer(MS coat) on microtensile bond strength of different adhesives: a three-step adhesive(All-Bond 2), a two-step adhesive(Single Bond), a one-step adhesive(One-up Bond F).
Non-caries extracted human molars were used. Dentin surface was obtained by horizontal section on midportion of crown using a water-cooled low speed diamond saw. Teeth were randomly divided into 6 group. AMO(MS coat + All Bond)-, SMO(MS coat + Single Bond)- and OMO(MS coat + One-up Bond F)-dentin surface were treated with 17% EDTA before bonded adhesive. AMX-, SMX- and OMX-dentin surface were bonded with All-Bond 2, Single Bond and One-up Bond F, respectively, with no previous treatment with MS coat and 17% EDTA. About 1cm high resin composite(Z-250™) were incrementally build-up on the treated surface. The specimens for the microtensile test were serially sectioned perpendicular to the adhesive layer to obtain 0.7×0.7 mm sticks. 30 sticks were prepared from each group.
After that, tensile bond strength for each stick was measured with Microtensile Tester at a 1mm/min crosshead speed. Fractured dentin surfaces were observed under the SEM. The results were statistically analysed by using a One-way ANOVA and Tukey's test(p<0.05).
Value in MPa were: AMO-44.35±13.21; SMO-39.35±13.32; OMO-31.07±10.25; AMX-49.22±16.38; SMX-56.02±13.35; OMX-72.93±16.19. Application of MS coat reduced microtensile bond strengths of both Single Bond and One-up Bond F, whereas microtensile bond strengths of All-Bond 2 were not affected significantly.
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Microtensile bonding of resin fiber reinforced post to radicular dentin using resin cement
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Jin-Woo Kim, Mi-Kyung Yu, Se-Joon Lee, Kwang-Won Lee
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J Korean Acad Conserv Dent 2003;28(1):80-88. Published online January 31, 2003
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DOI: https://doi.org/10.5395/JKACD.2003.28.1.080
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Abstract
PDFPubReaderePub
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Object
The purpose of this study were to evaluate the microtensile bond strength of resin fiber reinforced post to radicular dentin using resin cement according to various dentin surface treatment and to observe the interface between post and root dentin under SEM.
Material and Method
A total 16 extracted human single rooted teeth were used.
A lingual access was made using a #245 carbide bur in a high-speed handpiece with copious air water spray. The post space was mechanically enlarged using H-file(up to #60) and Gates Glidden bures(#3). This was followed by refining of the canal space using the calbrating drill set provided in ER Dentinpost(GEBR, BRASSELER GmbH&Co. KG).
The 16 teeth were randomly distributed into 4 group of 4 teeth.
Group 1 teeth had their post space prepared using 10% phosphoric acid as root canal surface treatment agent during 20s. The canal was then rinsed with saline and dried with paper point.
Group 2 teeth had their post space prepared using 3% NaOCl as root canal surface treatment agent during 30min. The canal was then rinsed with saline and dried with paper point.
Group 3 teeth had their post space prepared using 17% EDTA as root canal surface treatment agent during 1min. The canal was then rinsed with saline and dried with paper point.
Group 4 teeth had their post space prepared using 17% EDTA as root canal surface treatment agent during 1min. After rinsing with saline, the canal was rinced 10ml of 3% NaOCl for 30min.
After drying with paper point, the post(ER Dentinpost, GEBR, BRASSELER GmbH&Co. KG) was placed in the treated canals using resin cement. Once the canal was filled with resin cement(Super bond C&B sunmedical co. Ltd.), a lentulo was inserted to the depth of the canal to ensure proper coating of the root canal wall.
After 24 hours, acrylic resin blocks(10·10·50mm) were made. The resin block was serially sectioned vertically into stick of 1·1mm. Twenty sticks were prepared from each group. After that, tensile bond strengths for each stick was measured with Microtensile Tester.
Failure pattern of the specimen at the interface between post and dentin were observed under SEM.
Results
1. Tensile bond strengths(meen±SD) were expressed with ascending order as follows ; group 4, 12.52±6.60 ; group 1, 7.63±5.83 ; group 2, 4.13±2.31 ; group 3, 3.31±1.44.
2. Tensile bond strengths of Group 4 treated with 17% EDTA +3%NaOCl were significant higher than those of group 1, 2 and 3 (p<0.05).
3. Tensile bond strengths of Group 1 treated with 10% phosphoric acid were significant higher than those of group 2 (p<0.05). Tensile bond strengths of Group 4 treated with 17% EDTA +3% NaOCl was significant higher than those of other groups.
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Measurement of adhesion of root canal sealer to dentine and gutta-percha
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Mi-Ja Her, Mi-Kyung Yu, Se-Joon Lee, Kwang-Won Lee
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J Korean Acad Conserv Dent 2003;28(1):89-99. Published online January 31, 2003
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DOI: https://doi.org/10.5395/JKACD.2003.28.1.089
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Abstract
PDFPubReaderePub
The purpose of this study was to investigate the bonding of resin-based root canal sealer, AH26 when the sealer was applied as a thin layer between dentine and gutta-percha surface.
In this study, forty non-caries extracted human molars and resin-based root canal sealer(AH 26, DeTrey/Dentsply, Germany) were used. Disks of gutta-percha, 6mm in diameter·6mm thick (Diadent/Dentsply, Korea) for thermoplastic obturation were used and dentin surfaces were treated with 2% NaOCl(Group 1) or 2%NaOCl+17% EDTA(Group 3). Disks of gutta-percha, 6mm in diameter·6mm thick (Diadent/Dentsply, Korea) for conventional obturation were used and dentin surface were treated with 2% NaOCl(Group 2) or 2%NaOCl+17% EDTA(Group 4). Enamel was removed by a horizontal section 1mm below the deepest portion of the central occlusal groove by using a water-cooled low speed diamond saw. A second horizontal section was done around cementoenamel junction. Exposed dentin surface was cut to approximately 8×8 mm rectangular shape and was ground against 320, 400, 600 grade silicon carbide abrasive paper serially. After grinding, the dentine surface were soaked in a solution of 2% NaOCl for 30 minutes and twenty of specimens were treated with 17% EDTA solution for 1 minute. The treated specimens were washed and dried. Root canal sealer, AH26 was prepared according to the manufacture's instructions. The Gutta-percha and dentin surface were coated with a thin layer of the freshly mixed sealer. The specimens were left overnight at room temperature. After their initial set, they were transferred to an incubator at 37℃ for 72 h. After 72 hours, resin blocks were made. The resin block was serially sectioned vertically into stick of 1·1mm. Twenty sticks were prepared from each group.
After that, tensile bond strength for each stick was measured with Microtensile Tester. Failure patterns of the specimens at the interface between gutta-percha and dentin were observed under the SEM(×1000) and Stereomicroscope (LEICA M420, Meyer Inst., TX U.S.A) at 1.25 ×25 magnification. The results were statistically analysed by using a One-way ANOVA and Tukey's test.
The results were as follows;
1. Tensile bond strengths(mean±SD) were expressed with ascending order as follows: Group 1, 3.09±1.05MPa ; Group 2, 6.23±1.16MPa ; Group 3, 7.12±1.07MPa ; Group 4, 10.32±2.06MPa.
2. Tensile bond strengths of the group 2 and 4 used disks of gutta-percha for conventional obturation were significantly higher than that of the group 1 and 3 used for thermoplastic obturation. (p < 0.05)
3. Tensile bond strengths of the group 3 and 4 treated with 2% NaOCl+17% EDTA were significantly higher than that of the group 1 and 2 treated with 2% NaOCl. (p < 0.05)
4. In analysis of failure patterns at the interface between sealer and gutta-percha, there were observed 49 (61%)cases of adhesive failure patterns and 31(39%) cases of mixed failures patterns.
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COMPARISON OF CANAL SHAPING METHODS WITH GT™ ROTARY FILE AND CONDENSATION METHODS
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Yu-Mi Kang, Jeong-Hee Jin, Mi-Kyung Yu, Se-Joon Lee, Kwang-Won Lee
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J Korean Acad Conserv Dent 2002;27(5):521-529. Published online January 14, 2002
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DOI: https://doi.org/10.5395/JKACD.2002.27.5.521
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Abstract
PDFPubReaderePub
- ABSTRACT
The purpose of this study was to compare the shaping time of two shaping methods and the leakage of three different obturation techniques. Ninty three canaled human molar teeth were used, which were randomly divided into two groups of forty teeth each and ten control teeth. After working length determination, the one group was prepared crown-down technique using rotary root canal instruments of GT rotary files .12/20, .10/20, .08/20 and .06/20 taper(Maillefer Instrument SA. Switzerland). The other group was instrumented with Gates Glidden burs(#1, #2, and #3) to coronal preparation and GT rotary files .08/20 and .06/20 taper to apical preparation. Shaping time was measured.
After root canals were instrumented, they were divided to three subgroups and obturated as follows: Subgroup 1, obturated with single cone method : Subgroup 2, obturated with lateral condensation : Subgroup 3, obturated with continuous wave technique. Three subgroups were obturated using non-standardized gutta-percha cone(Diadent, Korea, .06 or .08 taper) and AH-26(Dentsply DeTrey, Germany) as a root canal cement.
Ten unobturated teeth served as positive and negative controls. After immersion in 2% methylene blue solution for 1 month, the teeth were washed during 24h. The teeth were demineralized in 10% nitric acid and dehydrated by immersion in 80, 90 and 100% ethyl alcohol. The teeth were finally cleared and stored in 100%methylsalicylate, and apical dye penetration was evaluated under stereomicroscope(Leica M420, LC, U.S.A)at ×8.75 magnification.
Liner measurement of dye penetration was assessed with the use of digitalized image analysing system (analySIS, GmbH, Germany). The data were analysed statistically using independent T-test and Two-way ANOVA and Tukey test. The result were as follows:
In canal prepared with GT™rotary file, shaphing time taked more than the group of using Gates Glidden drill to coronal preparation without statistical significance (p>0.05).
The group of single cone obturation using canal preparation of GT™ rotary files showed significantly more apical leakage than those of lateral condensation and continuous wave technique regardless of shaping method (p<0.05).
The group of single cone obturation using canal preparation of GT™ rotary files and Gates Glidden drill showed significantly more apical leakage than those of continuous wave technique regardless of shaping method (p<0.05).
Regardless of shaping method, The group of continuous wave obturation showed less apical leakage than those of lateral condensation without statistical significance (p>0.05).
The group of single cone obturation using canal preparation of GT™ rotary files and Gates Glidden drill showed more apical leakage than the group of lateral condensation using same shaping method without statistical significance (p>0.05).
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