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Department of Conservative Dentistry, School of Dentistry, Chonbuk National University, Korea.
Corresponding Author: Mi-Kyung Yu. Department of Conservative Dentistry, School of Dentistry, Chonbuk National University, Geumam-Dong, Deokjin-Gu, Jeonju, Jeonbuk 561-712, Korea. Tel: 82-63-250-2045, Fax: 82-63-250-2049, mkyou102@hanmail.net
• Received: June 24, 2009 • Revised: September 25, 2009 • Accepted: October 14, 2009
The purpose of this study was to evaluate the effect of soft chelating irrigant on the sealing ability of root fillings by using a glucose leakage test.
A total of 45 single-rooted teeth were selected for the study. The teeth were decoronated leaving a total length of 13mm. The root canals prepared using K3 NiTi rotary instruments to an apical dimension of size 45(0.06 taper). The specimens were then randomly divided into 3 experimental groups of 13 roots each and 2 control groups of 3 roots each. Specimen in each group were prepared with different irrigation protocols : group 1, 2.5% NaOCl; group 2, 2.5% NaOCl and 17% EDTA; group 3, 2.5% NaOCl and 15% HEBP. The root canals were filled with gutta-percha and AH Plus sealer using lateral condensation. After 7 days in 37℃, 100% humidity, the coronal-to-apical microleakage was evaluated quantitatively using a glucose leakage model. The leaked glucose concentration was measured with spectrophotometry at 1, 4, 7, 14, 21 and 28 days.
There was a tendency of increase in leakage in all experimental groups during experimental period. HEBP-treated dentin showed no significant difference with EDTA-treated dentin during experimental period. From the 21th day onward, HEBP-treated dentin showed significantly lower leakage than smear-covered dentin. HEBP-treated dentin displayed a similar sealing pattern to EDTA-treated dentin and a better sealing ability than smear-covered dentin. Consequently, a soft chelator(HEBP) could be considered as the possible alternative to EDTA.
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Figure 1
Glucose leakage model.
Figure 2
Leakage of positive and negative control groups.
Figure 3
Glucose microleakage of experimental groups.
Table 1
Microleakage of glucose in experimental groups (mM/L)