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Articles in E-pub version are posted online ahead of regular printed publication.

Research Articles
Influence of adjacent restorative material and distance on the accuracy of inlay cavity impressions with intraoral scanner: an in vitro study
So-Yeon Lee, Sung-Ae Son, Jae-Hoon Kim, Deog-Gyu Seo, Jeong-Kil Park
Published online January 23, 2026
DOI: https://doi.org/10.5395/rde.2026.51.e6    [Epub ahead of print]
AbstractAbstract PDFPubReaderePub
Objectives
This study aimed to evaluate the influence of adjacent restorative material and interproximal distance on the accuracy of digital impressions of inlay cavities obtained using an intraoral scanner.
Methods
A disto-occlusal inlay cavity was prepared on a mandibular right first molar model, and digital scans were performed using a CEREC Primescan (Dentsply Sirona). The adjacent restorative materials used were Lava (3M ESPE), ENAMIC (VITA Zahnfabrik), Celtra Duo (Dentsply Sirona), and DMAX (DMAX INTERNATIONAL), and the interproximal distances were set to 0.6 mm, 0.8 mm, and 1.0 mm. The obtained scan data were analyzed using GOM Inspect software (GOM GmbH).
Results
Trueness, maximum positive and negative deviations, and precision were significantly influenced by both the adjacent restorative material and the interproximal distance, while their interaction showed a significant effect only on precision. Celtra Duo demonstrated the highest trueness, with mean deviation values decreasing from 7.8 μm at a 0.6 mm interproximal distance to 7.3 μm at 1.0 mm. ENAMIC showed the best precision, presenting mean deviations of 2.6 μm at 0.6 mm, 2.9 μm at 0.8 mm, and 2.4 μm at 1.0 mm. A narrow interproximal distance of 0.6 mm resulted in lower trueness, measured at 8.3 μm, and the highest precision deviation of 3.4 μm. In contrast, an interproximal distance of 1.0 mm yielded improved scan accuracy, with increased trueness and reduced precision variation.
Conclusions
Digital impression accuracy of inlay cavities was influenced by adjacent restorative material and interproximal distance, suggesting clinical consideration is needed in CAD/CAM workflows to optimize restoration fit.
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Enhancing antimicrobial properties of a resin-based material via incorporation of a powdered phytotherapeutic extract: an in vitro experimental study
Rodolfo Xavier de Sousa-Lima, Maria Eduarda Lima do Nascimento Marinho, Janielly Cristina Costa da Silva, Moan Jéfter Fernandes Costa, Pedro Henrique Sette-de-Souza, Giana da Silveira Lima, Boniek Castillo Dutra Borges
Received March 25, 2025  Accepted September 11, 2025  Published online January 20, 2026  
DOI: https://doi.org/10.5395/rde.2026.51.e2    [Epub ahead of print]
AbstractAbstract PDFPubReaderePub
Objectives
This study aimed to evaluate the degree of conversion (DC), immediate enamel bond strength (IEBS), antimicrobial activity, and release of the active principle of a resin-based material (RBM) enriched with the powdered Schinopsis brasiliensis (Braúna) stem antibacterial extract.
Methods
The RBM was enriched with 0, 1.25, 2.5, 5, 10, and 20 wt% powdered Braúna extract. The DC (n = 7) was assessed using micro-Raman spectroscopy. The IEBS (n = 7) was determined through the microshear test until failure, and failure modes were examined under a stereomicroscope. The antimicrobial activity (n = 15) was assessed by quantifying colony-forming units, and the release of the active principle was determined using ultra-high-performance liquid chromatography. One-way analysis of variance/Tukey and Kruskal-Wallis/Dunn tests were utilized to analyze the data (p < 0.05).
Results
Materials with 10 wt% and 20 wt% extract showed the lowest DC statistically. However, for IEBS, there were no statistically significant differences among the different groups. All materials released the active principle, but only those with 20 wt% and 10 wt% extract could inhibit biofilm formation similarly to 0.12% chlorhexidine.
Conclusions
Adding powdered Braúna extract between 10 wt% and 20 wt% is a promising alternative to provide an antimicrobial function to RBMs.
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Analysis of the reciprocating kinematics of the VDW Silver Reciproc, E-Connect Pro, Ecom, and Endopen endodontic motors: an in vitro experimental study
Cristielly França, Juliana D. Bronzato, Dieimes Braambati, Adriana de-Jesus-Soares, Carla C. R. B. Félix, Michelle A. N. S. Ferreira, Marcos Frozoni
Received August 18, 2025  Accepted October 12, 2025  Published online January 20, 2026  
DOI: https://doi.org/10.5395/rde.2026.51.e5    [Epub ahead of print]
AbstractAbstract PDFPubReaderePub
Objectives
This study aimed to evaluate the actual parameters of four endodontic motors, each adjusted for reciprocating motion, and compare them to the manufacturers’ declared values.
Methods
The motors used were the VDW Silver Reciproc (VDW GmbH), E-Connect Pro (MK Life), Ecom (Woodpecker), and Endopen (Schuster Woodpecker). A custom optical target was attached to the motor contra-angle, the movements were recorded with a high-resolution camera, and the images were analyzed. Engagement, disengagement, net angles, and speed for each operation cycle, duration of clockwise (CW) and counter-clockwise (CCW) movement, duration of standstill after CW and CCW movement, and the number of cycles to complete a full rotation were analyzed. The data were statistically analyzed at a significance level of 5%. The replicability of all reciprocal parameters analyzed was statistically different from that reported by the manufacturers.
Results
There was no statistically significant difference between the VDW Silver Reciproc, Ecom, and Endopen for the engagement angle. The E-Connect Pro was the least reliable at the 150°/30° settings for both angle parameters. There was no significant difference between the set and actual cycle net angles for the VDW Silver Reciproc (p = 0.493). While the actual values for the Ecom and E-Connect Pro were significantly higher than the set (p < 0.001), the actual values for the Endopen were significantly lower than the set (p < 0.001).
Conclusions
Experiments on four commercially available reciprocating endodontic motors revealed that the actual motor values differed significantly from the set values.
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Effect of combined application of premixed bioceramic paste and diode laser in vital pulp therapy: an immunohistochemical randomized controlled split-mouth in vivo animal experiment
Mo’men A. Salama, Dalia M. Fayyad, Mohamed I. Rabie, Manar A. A. Selim, Mahmoud F. Ahmed
Published online January 20, 2026
DOI: https://doi.org/10.5395/rde.2026.51.e4    [Epub ahead of print]
AbstractAbstract PDFPubReaderePub
Objectives
This study aimed to evaluate the effect of premixed bioceramic paste (Well-Root PT; Vericom) compared to mineral trioxide aggregate (MTA) on the expression of the mineralization-related marker dentin sialoprotein (DSP) in dental pulp following direct pulp capping, with or without prior diode laser application.
Methods
Direct pulp exposures were performed in the upper and lower incisors of eight dogs (n = 96 teeth). Cavities (Class V) were created and received pulp capping with either Well-Root PT (n = 32), MTA (n = 32), or no capping material (Teflon disc only) (n = 32), with or without the application of a diode laser. Immunohistochemical analysis of DSP expression was conducted and quantified as the mean area percentage using ImageJ software at 2 and 8 weeks posttreatment.
Results
Both the Well-Root PT and MTA groups showed significantly increased DSP expression compared to the control group at both 2 and 8 weeks (p < 0.05). No significant difference in the mean area percentage of DSP expression was found between the Well-Root PT and MTA groups. The diode laser application did not produce a significant effect on DSP expression. Within-group comparison revealed a significant increase in DSP expression between the 2- and 8-week follow-up periods (p < 0.05).
Conclusions
Well-Root PT demonstrated comparable efficacy to MTA in promoting DSP expression, supporting its use as an effective direct pulp capping material. Diode laser application prior to capping had no effect on DSP expression in this experimental model.
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Ex vivo comparative analysis of retrievability among four calcium silicate-based sealers for regaining apical patency
Darian Shomali, Timothy Kirkpatrick, Sang Won Kwak, Hyeon-Cheol Kim, Ji Wook Jeong
Published online January 14, 2026
DOI: https://doi.org/10.5395/rde.2026.51.e3    [Epub ahead of print]
AbstractAbstract PDFPubReaderePub
Objectives
Efficient retrievability is a key requirement for endodontic sealers. This study evaluated the retrievability of four different calcium silicate-based sealers (CSS).
Methods
A total of 153 single-rooted human teeth with straight canals were decoronated to a standardized working length of 12 mm. The canals were negotiated to working length using K files up to size 15/.02, followed by rotary instrumentation up to 35/.04, 2 mm short of working length. The teeth were randomly assigned to five groups: NeoSEALER Flo (NEO; Avalon Biomed), Ceraseal (CS; Meta Biomed), Endosequence BC Sealer (BC; Brasseler USA), AH Plus Bioceramic Sealer (AHB; Dentsply Sirona), and a negative control group. Sealer application and obturation with a 35/.04 gutta-percha cone were performed. After incubation at 37°C in 100% humidity for 7 days, retreatment was performed until apical patency was obtained, with retrievability assessed by regaining apical patency. One-way analysis of variance and Tukey contrast test were used to determine whether there was a significant difference among the four different CSS (p < 0.05).
Results
Success rates in regaining apical patency were NEO (79.4%), CS (37.0%), BC (50.0%), and AHB (69.7%). NEO demonstrated the highest retrievability, while CS had the lowest (p < 0.01).
Conclusions
The type of CSS used has a considerable impact on retreatment difficulty. Among the tested sealers, NeoSEALER Flo showed the highest retrievability, making it the most retrievable CSS in terms of retreatment efficacy.
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Case Report
Fifty-year follow-up of dens invaginatus treated by nonsurgical and surgical endodontic treatments: a case report
Qais Arow, Eyal Rosen, Galit Sela, Shlomo Elbahary, Igor Tsesis
Received April 9, 2025  Accepted August 26, 2025  Published online December 18, 2025  
DOI: https://doi.org/10.5395/rde.2026.51.e1    [Epub ahead of print]
AbstractAbstract PDFPubReaderePub
This case report presents a lateral maxillary incisor with dens invaginatus (DI) type IIIb that was treated both nonsurgically and surgically over 50 years. Treatment of teeth with DI can be challenging. Suggested options may include nonsurgical root canal treatment, endodontic surgery, or extraction. In this case report, a 13-year-old patient with a lateral maxillary incisor with DI type IIIb was treated by nonsurgical root canal treatment, modern endodontic surgery, and reoperation over the course of 50 years. There was complete healing at the last follow-up, 11 years after the reoperation. Correct diagnosis and proper treatment using modern endodontic techniques can enable teeth with DI to survive throughout the life span of the patient.
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