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Review Article
Disinfectant effectiveness of chlorhexidine gel compared to sodium hypochlorite: a systematic review with meta-analysis
Theodoro Weissheimer, Karem Paula Pinto, Emmanuel João Nogueira Leal da Silva, Lina Naomi Hashizume, Ricardo Abreu da Rosa, Marcus Vinicius Reis Só
Restor Dent Endod 2023;48(4):e37.   Published online October 26, 2023
DOI: https://doi.org/10.5395/rde.2023.48.e37
AbstractAbstract PDFSupplementary MaterialPubReaderePub

This study aimed to compare the disinfectant ability of chlorhexidine (CHX) gel and sodium hypochlorite (NaOCl). Systematic searches were conducted from inception until December 8th, 2022 (MEDLINE/PubMed, Cochrane Library, Web of Science, Scopus, Embase, and Grey Literature databases). Only randomized clinical trials were included. The revised Cochrane risk of bias tools for randomized trials were used to assess the quality of studies. Meta-analyses were performed. The overall quality of evidence was assessed through the Grading of Recommendations Assessment, Development, and Evaluation tool. Six studies were included. Five had a low risk of bias and 1 had some concerns. Three studies assessed bacterial reduction. Two were included in the meta-analysis for bacterial reduction (mean difference, 75.03 [confidence interval, CI, −271.15, 421.22], p = 0.67; I2 = 74%); and 3 in the meta-analysis for cultivable bacteria after chemomechanical preparation (odds ratio, 1.03 [CI, 0.20, 5.31], P = 0.98; I2 = 49%). Five studies assessed endotoxin reduction. Three were included in a meta-analysis (mean difference, 20.59 [CI, −36.41, 77.59], p = 0.48; I2 = 74%). There seems to be no difference in the disinfectant ability of CHX gel and NaOCl, but further research is necessary.

Citations

Citations to this article as recorded by  
  • Preparing porcine lens to mimic human lens capsule
    Yajing Pei, Shaofeng Han, Mingfeng Lu, Yang Yang, Ke Ma
    Journal of Cataract & Refractive Surgery.2024; 50(9): 963.     CrossRef
  • Comparative Evaluation of Disinfection Protocols for Dental Impressions in Prosthodontics
    Subhash Sonkesriya, Ghanshyam Gaur, Akanksha Maheshwari, Arun Kumar Ashahiya, Simran Kaur Aulakh, Amit Kumar, Bhumika Kamal Badiyani
    Cureus.2024;[Epub]     CrossRef
  • 394 View
  • 19 Download
  • 2 Web of Science
  • 2 Crossref
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Research Articles
Comparison of the sealing ability of various bioceramic materials for endodontic surgery
Benjamin Rencher, Ana M. Chang, Hanson Fong, James D. Johnson, Avina Paranjpe
Restor Dent Endod 2021;46(3):e35.   Published online June 8, 2021
DOI: https://doi.org/10.5395/rde.2021.46.e35
AbstractAbstract PDFPubReaderePub
Objectives

Endosequence Bioceramic Root Repair Material (BC-RRM) is used in endodontic microsurgery. It is available as a paste and a putty. However, no studies to date have examined the sealing ability of these forms alone or in combination as root-end filling materials. Hence, this study aimed to compare the sealing properties of these 2 forms of BC-RRM.

Materials and Methods

Forty-two extracted upper anterior teeth were divided into 3 experimental groups, a positive and negative control. After the root canal treatment, the root ends were resected, retroprepared and retrofilled with either putty, paste + putty or mineral trioxide aggregate (MTA). The teeth were mounted in tubes so the apical 3 mm was submerged in Brain Heart Infusion (BHI) broth. The coronal portions of the canals were inoculated with Enterococcus faecalis and BHI broth and incubated for 30 days. The broth in the tubes was analyzed for colony forming units to check for leakage of bacteria from the canal. The teeth from the groups were sectioned and analyzed using scanning electron microscopy (SEM). The Kruskal-Wallis test and analysis of variance were used to analyze the data with a significance level p < 0.05.

Results

The BC-RRM and MTA groups showed similar sealing ability. The positive control showed leakage in all samples. The SEM imaging showed the presence of bacteria in all experimental groups at the material-tooth interface.

Conclusions

No significant differences were noted in the experimental groups, providing sufficient evidence that any combination could be effectively used during endodontic microsurgery.

Citations

Citations to this article as recorded by  
  • An Ex-vivo Evaluation of Sealability of Three Bioceramic Physical Variants in Coronal and Apical Thirds of Root Canals
    Murali H Rao, Rajkumar Krishnan, Pavithra Gopal, Elizabeth Thomas
    The Journal of Contemporary Dental Practice.2025; 25(11): 1022.     CrossRef
  • Clinical applications and classification of calcium silicate-based cements based on their history and evolution: a narrative review
    Kenta Tsuchiya, Salvatore Sauro, Hidehiko Sano, Jukka P. Matinlinna, Monica Yamauti, Shuhei Hoshika, Yu Toida, Rafiqul Islam, Atsushi Tomokiyo
    Clinical Oral Investigations.2025;[Epub]     CrossRef
  • Evaluation of the Marginal Adaptation of Two Hydraulic Calcium Silicate Cements Used in Apical Plugs: An In Vitro Study
    Sara Filipe, José Pedro Martinho, Siri Paulo, Catarina Carvalho, Ana Coelho, Inês Amaro, Eunice Carrilho, Anabela Paula, Carlos Miguel Marto, Henrique Girão, Mónica Zuzarte, Ana S. Pires, Manuel Marques Ferreira
    Applied Sciences.2024; 14(2): 480.     CrossRef
  • A Study on Nanoleakage of Apical Retrograde Filling of Premixed Calcium Silicate-Based Cement Using a Lid Technique
    Nyamsuren Enkhbileg, Jin Woo Kim, Seok Woo Chang, Se-Hee Park, Kyung Mo Cho, Yoon Lee
    Materials.2024; 17(10): 2366.     CrossRef
  • The outcome of combined use of iRoot BP Plus and iRoot SP for root-end filling in endodontic microsurgery: a randomized controlled trial
    Xu Dong, Qin Su, Wen Li, Jinbo Yang, Dongzhe Song, Jing Yang, Xin Xu
    Clinical Oral Investigations.2024;[Epub]     CrossRef
  • Bacterial sealing ability of calcium silicate-based sealer for endodontic surgery: an in-vitro study
    Mai M. Mansour, Sybel M. Moussa, Marwa A. Meheissen, Mahmoud R. Aboelseoud
    BMC Oral Health.2024;[Epub]     CrossRef
  • Evaluation of Marginal Adaptation of Three Biomaterials as Apical Barrier in Experimental Apexification Model
    Nagehan Aktaş, Didem Sakaryalı Uyar, Didem Atabek
    ADO Klinik Bilimler Dergisi.2024; 13(3): 409.     CrossRef
  • In vitro evaluation of the sealing ability of combined use of iRoot BP Plus and iRoot SP for root-end filling
    Xu Dong, Qian Xie, Xin Xu
    Clinical Oral Investigations.2023; 27(6): 2969.     CrossRef
  • Outcomes of endodontic microsurgery using different calcium silicate–based retrograde filling materials: a cohort retrospective cone-beam computed tomographic analysis
    Rawan F. Eskandar, Mey A. Al-Habib, Mohammed A. Barayan, Hadeel Y. Edrees
    BMC Oral Health.2023;[Epub]     CrossRef
  • Bioceramics in Endodontics: Updates and Future Perspectives
    Xu Dong, Xin Xu
    Bioengineering.2023; 10(3): 354.     CrossRef
  • Biological properties of Ceraputty as a retrograde filling material: an in vitro study on hPDLSCs
    Sergio López-García, Francisco J. Rodríguez-Lozano, José Luis Sanz, Leopoldo Forner, María Pilar Pecci-Lloret, Adrián Lozano, Laura Murcia, Sonia Sánchez-Bautista, Ricardo E. Oñate-Sánchez
    Clinical Oral Investigations.2023; 27(8): 4233.     CrossRef
  • Bone Window Technique in Endodontic Microsurgery – Report of Two Cases
    Spyros Floratos, Vasileios Molonis, Apostolos Tsolakis, Stylianos Kykalos, Konstantinos Kontzoglou
    Journal of Endodontic Microsurgery.2022; 2: 24.     CrossRef
  • 478 View
  • 28 Download
  • 10 Web of Science
  • 12 Crossref
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Influence of reciprocating and rotary instrumentation on microbial reduction: a systematic review and meta-analysis of in vitro studies
Selen Küçükkaya Eren, Emel Uzunoğlu-Özyürek, Sevilay Karahan
Restor Dent Endod 2021;46(2):e19.   Published online March 10, 2021
DOI: https://doi.org/10.5395/rde.2021.46.e19
AbstractAbstract PDFPubReaderePub
Objectives

The purpose of this study was to conduct a systematic review and meta-analysis of in vitro studies regarding the effectiveness of reciprocating and rotary instrumentation on microbial reduction in root canals.

Materials and Methods

PubMed, Scopus, Web of Science, the Cochrane Library, and the gray literature were searched through December 2019. Studies comparing the influence of reciprocating and rotary instrumentation on the removal of microorganisms from root canals that quantified the antimicrobial effect were included. Data extraction was completed using a systematic form for data collection. The risk of bias of the studies was evaluated. Standardized mean differences (SMDs) and confidence intervals (CIs) were calculated using a random effects meta-analysis.

Results

Seventeen in vitro studies were included in this systematic review, of which 7 provided adequate data for inclusion in the meta-analysis. Both reciprocating and rotary systems were similarly effective in reducing the microbial load in infected root canals (SMD [95% CI], 0.0481 [−0.271, 0.367]). Three studies showed a low risk of bias, whereas most of the studies (82%) presented a medium risk.

Conclusions

Although both techniques decrease the microbial content (with reductions of 23.32%–88.47% and 23.33%–89.86% for reciprocating and rotary instrumentation, respectively), they are not able to provide complete disinfection of root canals.

Citations

Citations to this article as recorded by  
  • Fifteen years of engine‐driven nickel–titanium reciprocating instruments, what do we know so far? An umbrella review
    Felipe Immich, Lucas Peixoto de Araújo, Rafaella Rodrigues da Gama, Wellington Luiz de Oliveira da Rosa, Evandro Piva, Giampiero Rossi‐Fedele
    Australian Endodontic Journal.2024; 50(2): 409.     CrossRef
  • Does minimally invasive canal preparation provide higher fracture resistance of endodontically treated teeth? A systematic review ofin vitrostudies
    Sıla Nur Usta, Emmanuel João Nogueira Leal Silva, Seda Falakaloğlu, Mustafa Gündoğar
    Restorative Dentistry & Endodontics.2023;[Epub]     CrossRef
  • The Effect of Combined Ultrasonic Tip and Mechanized Instrumentation on the Reduction of the Percentage of Non-Instrumented Surfaces in Oval/Flat Root Canals: A Systematic Review and Meta-Analysis
    Marcella Dewes Cassal, Pedro Cardoso Soares, Marcelo dos Santos
    Cureus.2023;[Epub]     CrossRef
  • Impact of Different Access Cavity Designs and Ni–Ti Files on the Elimination of Enterococcus faecalis from the Root Canal System: An In Vitro Study
    Gizem Andac, Atakan Kalender, Buket Baddal, Fatma Basmaci
    Applied Sciences.2022; 12(4): 2049.     CrossRef
  • Shaping Properties and Outcomes of Nickel-Titanium Reciprocation Systems in Primary Teeth: A Systematic Review and Meta-Analysis of In Vitro Studies
    SelvaKumar Haridoss, Bhavyaa R, Kavitha Swaminathan, Aruna P
    Cureus.2022;[Epub]     CrossRef
  • Influence of Root Canal Sealers and Obturation Techniques on Vertical Root Fracture Resistance. An In Vitro Experiment
    Mazen F. Alkahtany, Khalid H. Almadi, Fahad A. Alahmad, Abdullah M. Alshehri, Abdulrahman A. AlSwayyed, Omar M. AlZahran, Ali AlHadan, Abdulaziz S. Almustafa, Fahim Vohra, Tariq Abduljabbar
    Applied Sciences.2021; 11(17): 8022.     CrossRef
  • 237 View
  • 12 Download
  • 6 Web of Science
  • 6 Crossref
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In vitro evaluation of octenidine as an antimicrobial agent against Staphylococcus epidermidis in disinfecting the root canal system
Jia Da Chum, Darryl Jun Zhi Lim, Sultan Omer Sheriff, Shaju Jacob Pulikkotil, Anand Suresh, Fabian Davamani
Restor Dent Endod 2019;44(1):e8.   Published online February 8, 2019
DOI: https://doi.org/10.5395/rde.2019.44.e8
AbstractAbstract PDFPubReaderePub
Objectives

Irrigants are imperative in endodontic therapy for the elimination of pathogens from the infected root canal. The present study compared the antimicrobial efficacy of octenidine dihydrochloride (OCT) with chlorhexidine (CHX) and sodium hypochlorite (NaOCl) against Staphylococcus epidermidis (S. epidermidis) for root canal disinfection.

Materials and Methods

The minimum inhibitory concentration (MIC) was obtained using serial dilution method. The agar diffusion method was then used to determine the zones of inhibition for each irrigant. Lastly, forty 6-mm dentin blocks were prepared from human mandibular premolars and inoculated with S. epidermidis. Samples were randomly divided into 4 groups of 10 blocks and irrigated for 3 minutes with saline (control), 2% CHX, 3% NaOCl, or 0.1% OCT. Dentin samples were then collected immediately for microbial analysis, including an analysis of colony-forming units (CFUs).

Results

The MICs of each tested irrigant were 0.05% for CHX, 0.25% for NaOCl, and 0.0125% for OCT. All tested irrigants showed concentration-dependent increase in zones of inhibition, and 3% NaOCl showed the largest zone of inhibition amongst all tested irrigants (p < 0.05). There were no significant differences among the CFU measurements of 2% CHX, 3% NaOCl, and 0.1% OCT showing complete elimination of S. epidermidis in all samples.

Conclusions

This study showed that OCT was comparable to or even more effective than CHX and NaOCl, demonstrating antimicrobial activity at low concentrations against S. epidermidis.

Citations

Citations to this article as recorded by  
  • WITHDRAWN: Substantivity of different antiseptic oral gels. An In vitro study
    Nirit Tagger Green, Roni Kolerman, Carlos Nemcovsky, Shlomo Matalon, Dan Gaukhman, Liat Chaushu
    Heliyon.2025; : e42654.     CrossRef
  • Evaluation of postoperative pain in endodontic retreatment with apical periodontitis using ozonated 2% chlorhexidine and 0.1% octenidine application: A randomized clinical trial
    Nidhi Sinha, Geeta Asthana, Girish Parmar, Akshayraj Langaliya, Jinali Shah, Bijay Singh
    Journal of Conservative Dentistry and Endodontics.2024; 27(6): 654.     CrossRef
  • Research on NiTi instruments combined with ultrasonic irrigation and multiantibiotic paste in root canal therapy of periapical inflammation in deciduous teeth
    Zongxia Zhu, Guangli Fu
    Experimental and Therapeutic Medicine.2024;[Epub]     CrossRef
  • Comparative evaluation of antimicrobial efficacy of 0.1% octenidine dihydrochloride, superoxidized solution, ozonated water, 0.1% silver nanoparticle solution, and Q mix™ 2 in 1 in root canals infected with Enterococcus faecalis
    Mahenaz Salam Inamdar, Dayanand G. Chole, Shrinivas S. Bakle, Preeti B. Vaprani, Neha P. Gandhi, Nikhil R. Hatte
    Journal of Conservative Dentistry and Endodontics.2024; 27(10): 1059.     CrossRef
  • Causal relationship, shared genes between rheumatoid arthritis and pulp and periapical disease: evidence from GWAS and transcriptome data
    Huili Wu, Lijuan Wang, Chenjie Qiu
    Frontiers in Immunology.2024;[Epub]     CrossRef
  • The effect of octenidine dihydrochloride on the antibacterial activity of a formulated resin composite: an in vitro study
    Mahitab Mansour, Tarek Salah, Haidy N. Salem
    Bulletin of the National Research Centre.2024;[Epub]     CrossRef
  • Association between pulp and periapical disease with type 2 diabetes: A bidirectional Mendelian randomization
    Yuqiang Wang, Jiakang Zhu, Ying Tang, Cui Huang
    International Endodontic Journal.2024; 57(5): 566.     CrossRef
  • New Insights Regarding the Use of Relevant Synthetic Compounds in Dentistry
    Stefania-Irina Dumitrel, Anamaria Matichescu, Stefania Dinu, Roxana Buzatu, Ramona Popovici, Dorin Dinu, Dana Bratu
    Molecules.2024; 29(16): 3802.     CrossRef
  • Formulation and Characterization of a Novel Palm-Oil-Based α-Mangostin Nano-Emulsion (PO-AMNE) as an Antimicrobial Endodontic Irrigant: An In Vitro Study
    Omer Sheriff Sultan, Haresh Kumar A/L Kantilal, Khoo Suan Phaik, Hira Choudhury, Fabian Davamani
    Processes.2023; 11(3): 798.     CrossRef
  • Comparative Evaluation of Antimicrobial Efficacy of Herbal Formulations of Septilin and Triphala with Conventional 2% Chlorhexidine on Root Canal and Oral Commensal Bacteria using Kirby Bauer Method
    Shadab Ahmed, Kamil Shahnawaz, Tapan Kumar Mandal, Mamnoon Ghafir, Shiva Shankar Gummaluri, Gaurav Vishal
    Contemporary Clinical Dentistry.2022; 13(4): 383.     CrossRef
  • A comparative assessment of pomegranate extract, sodium hypochlorite, chlorhexidine, Myrrh (Commiphora molmol), tulsi extract against Enterococcus faecalis, Fusobacterium nucleatum and Staphylococci epidermidis
    Mallwika Sisodiya, Shadab Ahmed, Ranjan Sengupta, Priyanka, Ankit Kumar Saha, Gourav Verma
    Journal of Oral and Maxillofacial Pathology.2021; 25(2): 369.     CrossRef
  • Effects of Octenidine on the Formation and Disruption of Dental Biofilms: An Exploratory In Situ Study in Healthy Subjects
    B. Reda, J. Dudek, M. Martínez-Hernández, M. Hannig
    Journal of Dental Research.2021; 100(9): 950.     CrossRef
  • Does Cavity Disinfectant Affect Sealing Ability of Universal Self-etch Adhesive?
    S Lata, Prasanti Kumari Pradhan, Gaurav Patri, Subhasmita Bhol, Kanhu C Sahoo, Khushboo Ghosh
    The Journal of Contemporary Dental Practice.2021; 22(3): 273.     CrossRef
  • Effect of duration and dilution on antimicrobial efficacy of octenidine hydrochloride as an intracanal medicament with chitosan carrier against Enterococcus faecalis – A modified direct contact test
    VinayaSusan Varghese, Nirmal Kurian
    Journal of Conservative Dentistry.2020; 23(5): 463.     CrossRef
  • 251 View
  • 5 Download
  • 14 Crossref
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Bacterial leakage and micro-computed tomography evaluation in round-shaped canals obturated with bioceramic cone and sealer using matched single cone technique
Kallaya Yanpiset, Danuchit Banomyong, Kanet Chotvorrarak, Ratchapin Laovanitch Srisatjaluk
Restor Dent Endod 2018;43(3):e30.   Published online July 5, 2018
DOI: https://doi.org/10.5395/rde.2018.43.e30
AbstractAbstract PDFPubReaderePub
Objectives

To evaluate sealing ability of root canals obturated with bioceramic-impregnated gutta percha cone (BCC) or gutta percha (GP), with bioceramic sealer (BCS) or AH Plus (AH; Dentsply-Maillefer), in roundly-prepared canals using matched single-cone technique, based on bacterial leakage test, and to analyze obturation quality using micro-computed tomography (CT) analysis.

Materials and Methods

Ninety-two distobuccal roots of maxillary molars were prepared using nickel-titanium files to apical size 40/0.06. The roots were divided into 4 groups (n = 20) that were obturated with a master cone and sealer: GP/AH, BCC/AH, GP/BCS, and BCC/BCS. Bacterial leakage model using Enterococcus faecalis was used to evaluate sealing ability for 60-day period. Obturated samples from each group (n = 4) were analyzed using micro-CT.

Results

All groups showed bacterial leakage at 20%–45% of samples with mean leakage times of 42–52 days. There were no significant differences in bacterial leakage among the groups. Micro-CT showed minimal gaps and voids in all groups at less than 1%.

Conclusions

In roundly-prepared canals, the single cone obturation with BCC/BCS was comparable to GP/AH for bacterial leakage at 60 days.

Citations

Citations to this article as recorded by  
  • In vitro comparative evaluation of apical leakage using a bioceramic sealer with three different obturating techniques: A glucose leakage model
    Tanvi S Agrawal, Shishir Singh, Rajesh S Podar, Gaurav Kulkarni, Anuprita Gadkari, Navin Agarwal
    Journal of Conservative Dentistry and Endodontics.2024; 27(1): 76.     CrossRef
  • In Vitro Microscopical and Microbiological Assessment of the Sealing Ability of Calcium Silicate-Based Root Canal Sealers
    Karin Christine Huth, Sabina Noreen Wuersching, Leander Benz, Stefan Kist, Maximilian Kollmuss
    Journal of Functional Biomaterials.2024; 15(11): 341.     CrossRef
  • Comparison between AH plus sealer and total fill bioceramic sealer performance in previously untreated and retreatment cases of maxillary incisors with large-sized periapical lesion: a randomized controlled trial
    Eisa Wahbi, Hassan Achour, Yasser Alsayed Tolibah
    BDJ Open.2024;[Epub]     CrossRef
  • Bacterial sealing ability of calcium silicate-based sealer for endodontic surgery: an in-vitro study
    Mai M. Mansour, Sybel M. Moussa, Marwa A. Meheissen, Mahmoud R. Aboelseoud
    BMC Oral Health.2024;[Epub]     CrossRef
  • Assessment the bioactivity of zinc oxid eugenol sealer after the addition of different concentrations of nano hydroxyapatite-tyrosine amino acid
    Rasha M. Al-Shamaa, Raghad A. Al-Askary
    Brazilian Journal of Oral Sciences.2024; 23: e243733.     CrossRef
  • Assessment of Bacterial Sealing Ability of Two Different Bio-Ceramic Sealers in Single-Rooted Teeth Using Single Cone Obturation Technique: An In Vitro Study
    Doaa M. AlEraky, Ahmed M. Rahoma, Hatem M. Abuohashish, Abdullh AlQasser, Abbas AlHamali, Hussain M. AlHussain, Hussain M. AlShoalah, Zakrya AlSaghah, Abdulrahman Khattar, Shimaa Rifaat
    Applied Sciences.2023; 13(5): 2906.     CrossRef
  • How do imaging protocols affect the assessment of root-end fillings?
    Fernanda Ferrari Esteves Torres, Reinhilde Jacobs, Mostafa EzEldeen, Karla de Faria-Vasconcelos, Juliane Maria Guerreiro-Tanomaru, Bernardo Camargo dos Santos, Mário Tanomaru-Filho
    Restorative Dentistry & Endodontics.2022;[Epub]     CrossRef
  • The impact of Morse taper implant design on microleakage at implant-healing abutment interface
    Soyeon KIM, Joo Won LEE, Jae-Heon KIM, Van Mai TRUONG, Young-Seok PARK
    Dental Materials Journal.2022; 41(5): 767.     CrossRef
  • A critical analysis of research methods and experimental models to study root canal fillings
    Gustavo De‐Deus, Erick Miranda Souza, Emmanuel João Nogueira Leal Silva, Felipe Gonçalves Belladonna, Marco Simões‐Carvalho, Daniele Moreira Cavalcante, Marco Aurélio Versiani
    International Endodontic Journal.2022; 55(S2): 384.     CrossRef
  • Micro‐CT assessment of gap‐containing areas along the gutta‐percha‐sealer interface in oval‐shaped canals
    Gustavo De‐Deus, Gustavo O. Santos, Iara Zamboni Monteiro, Daniele M. Cavalcante, Marco Simões‐Carvalho, Felipe G. Belladonna, Emmanuel J. N. L. Silva, Erick M. Souza, Raphael Licha, Carla Zogheib, Marco A. Versiani
    International Endodontic Journal.2022; 55(7): 795.     CrossRef
  • Comparison of Sealing Ability of Bioceramic Sealer, AH Plus, and GuttaFlow in Conservatively Prepared Curved Root Canals Obturated with Single-Cone Technique: An In vitro Study
    Shalan Kaul, Ajay Kumar, Bhumika Kamal Badiyani, Laxmi Sukhtankar, M. Madhumitha, Amit Kumar
    Journal of Pharmacy and Bioallied Sciences.2021; 13(Suppl 1): S857.     CrossRef
  • Micro-CT Evaluation of Four Root Canal Obturation Techniques
    Mahmood Reza Kalantar Motamedi, Amin Mortaheb, Maryam Zare Jahromi, Brett E. Gilbert, Marilena Vivona
    Scanning.2021; 2021: 1.     CrossRef
  • Effects of Both Fiber Post/Core Resin Construction System and Root Canal Sealer on the Material Interface in Deep Areas of Root Canal
    Hiroki Miura, Shinji Yoshii, Masataka Fujimoto, Ayako Washio, Takahiko Morotomi, Hiroshi Ikeda, Chiaki Kitamura
    Materials.2021; 14(4): 982.     CrossRef
  • Sealing ability and microbial leakage of root-end filling materials: MTA versus epoxy resin: A systematic review and meta-analysis
    Mario Dioguardi, Mario Alovisi, Diego Sovereto, Giuseppe Troiano, Giancarlo Malagnino, Michele Di Cosola, Angela Pia Cazzolla, Luigi Laino, Lorenzo Lo Muzio
    Heliyon.2021; 7(7): e07494.     CrossRef
  • Development of A Nano-Apatite Based Composite Sealer for Endodontic Root Canal Filling
    Angelica Bertacci, Daniele Moro, Gianfranco Ulian, Giovanni Valdrè
    Journal of Composites Science.2021; 5(1): 30.     CrossRef
  • BIOCERAMIC-BASED ROOT CANAL SEALERS
    L Somolová, Z Zapletalová, M Rosa, B Novotná, I Voborná, Y Morozova
    Česká stomatologie a praktické zubní lékařství.2021; 121(4): 116.     CrossRef
  • Calcium Silicate-Based Root Canal Sealers: A Narrative Review and Clinical Perspectives
    Germain Sfeir, Carla Zogheib, Shanon Patel, Thomas Giraud, Venkateshbabu Nagendrababu, Frédéric Bukiet
    Materials.2021; 14(14): 3965.     CrossRef
  • Physico-Chemical Properties of Calcium-Silicate vs. Resin Based Sealers—A Systematic Review and Meta-Analysis of Laboratory-Based Studies
    Viresh Chopra, Graham Davis, Aylin Baysan
    Materials.2021; 15(1): 229.     CrossRef
  • Comparison of apical sealing ability of bioceramic sealer and epoxy resin-based sealer using the fluid filtration technique and scanning electron microscopy
    Widcha Asawaworarit, Thitapa Pinyosopon, Kanittha Kijsamanmith
    Journal of Dental Sciences.2020; 15(2): 186.     CrossRef
  • Micro-computed tomographic evaluation of a new system for root canal filling using calcium silicate-based root canal sealers
    Mario Tanomaru-Filho, Fernanda Ferrari Esteves Torres, Jader Camilo Pinto, Airton Oliveira Santos-Junior, Karina Ines Medina Carita Tavares, Juliane Maria Guerreiro-Tanomaru
    Restorative Dentistry & Endodontics.2020;[Epub]     CrossRef
  • A micro-computed tomographic evaluation of root canal filling with a single gutta-percha cone and calcium silicate sealer
    Jong Cheon Kim, Maung Maung Kyaw Moe, Sung Kyo Kim
    Restorative Dentistry & Endodontics.2020;[Epub]     CrossRef
  • Comparative evaluation of sealing ability of gutta percha and resilon as root canal filling materials- a systematic review
    Pragya Pandey, Himanshi Aggarwal, A.P. Tikku, Arpit Singh, Rhythm Bains, Shambhavi Mishra
    Journal of Oral Biology and Craniofacial Research.2020; 10(2): 220.     CrossRef
  • Micro-computed tomographic evaluation of the flow and filling ability of endodontic materials using different test models
    Fernanda Ferrari Esteves Torres, Juliane Maria Guerreiro-Tanomaru, Gisselle Moraima Chavez-Andrade, Jader Camilo Pinto, Fábio Luiz Camargo Villela Berbert, Mario Tanomaru-Filho
    Restorative Dentistry & Endodontics.2020;[Epub]     CrossRef
  • Root fillings with a matched-taper single cone and two calcium silicate–based sealers: an analysis of voids using micro-computed tomography
    Eugenio Pedullà, Roula S. Abiad, Gianluca Conte, Giusy R. M. La Rosa, Ernesto Rapisarda, Prasanna Neelakantan
    Clinical Oral Investigations.2020; 24(12): 4487.     CrossRef
  • Influence of different disinfection protocols on gutta-percha cones surface roughness assessed by two different methods
    A.M. Nunes, J.P. Gouvea, L. da Silva
    Journal of Materials Research and Technology.2019; 8(6): 5464.     CrossRef
  • Endodontic sealers based on calcium silicates: a systematic review
    David Donnermeyer, Sebastian Bürklein, Till Dammaschke, Edgar Schäfer
    Odontology.2019; 107(4): 421.     CrossRef
  • 274 View
  • 7 Download
  • 26 Crossref
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Comparison of apical extrusion of intracanal bacteria by various glide-path establishing systems: an in vitro study
Alberto Dagna, Rashid El Abed, Sameeha Hussain, Ibrahim H Abu-Tahun, Livia Visai, Federico Bertoglio, Floriana Bosco, Riccardo Beltrami, Claudio Poggio, Hyeon-Cheol Kim
Restor Dent Endod 2017;42(4):316-323.   Published online October 31, 2017
DOI: https://doi.org/10.5395/rde.2017.42.4.316
AbstractAbstract PDFPubReaderePub
Objectives

This study compared the amount of apically extruded bacteria during the glide-path preparation by using multi-file and single-file glide-path establishing nickel-titanium (NiTi) rotary systems.

Materials and Methods

Sixty mandibular first molar teeth were used to prepare the test apparatus. They were decoronated, blocked into glass vials, sterilized in ethylene oxide gas, infected with a pure culture of Enterococcus faecalis, randomly assigned to 5 experimental groups, and then prepared using manual stainless-steel files (group KF) and glide-path establishing NiTi rotary files (group PF with PathFiles, group GF with G-Files, group PG with ProGlider, and group OG with One G). At the end of canal preparation, 0.01 mL NaCl solution was taken from the experimental vials. The suspension was plated on brain heart infusion agar and colonies of bacteria were counted, and the results were given as number of colony-forming units (CFU).

Results

The manual instrumentation technique tested in group KF extruded the highest number of bacteria compared to the other 4 groups (p < 0.05). The 4 groups using rotary glide-path establishing instruments extruded similar amounts of bacteria.

Conclusions

All glide-path establishment instrument systems tested caused a measurable apical extrusion of bacteria. The manual glide-path preparation showed the highest number of bacteria extruded compared to the other NiTi glide-path establishing instruments.

Citations

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    Murtada Qadir Muhaibes, Shatha Abdulkareem Alwakeel
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    Niranjan Desai, Ashish S Bhadane, Nishant K Vyavahare, Dipali Y Shah, Akash S Kale, Simran K Chaudhari
    Journal of Operative Dentistry & Endodontics.2024; 8(1): 1.     CrossRef
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    Zeliha Danaci, Kübra Yeşildal Yeter
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    Vlad Mircea Lup, Olivia Andreea Marcu, Carlo Gaeta, Gabriela Ciavoi
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    Maha Adnan Habeeb
    Journal of Orofacial Sciences.2022; 14(2): 93.     CrossRef
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    Jale Tanalp
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    Murilo Priori Alcalde, Marco Antonio Hungaro Duarte, Pedro Henrique Souza Calefi, Victor de Moraes Cruz, Bruno Carvalho de Vasconcelos, Marcus Vinícius Reis Só, Rodrigo Ricci Vivan
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Antibacterial effect of urushiol on E. faecalis as a root canal irrigant
Sang-Wan Kim, Dong-Hoon Shin
Restor Dent Endod 2017;42(1):54-59.   Published online January 25, 2017
DOI: https://doi.org/10.5395/rde.2017.42.1.54
AbstractAbstract PDFPubReaderePub
Objectives

The purpose of this study was to compare the antibacterial activity of urushiol against Enterococcus faecalis (E. faecalis) to that of NaOCl.

Materials and Methods

The canals of thirty two single rooted human teeth were instrumented with Ni-Ti files (ProTaper Next X1, X2, X3, Dentsply). A pure culture of E. faecalis ATCC 19433 was prepared in sterile brain heart infusion (BHI) broth. The teeth were submerged in the suspension of E. faecalis and were incubated at 37℃ for 7 days to allow biofilm formation. The teeth were randomly divided into three experimental groups according to the irrigant used, and a negative control group where no irrigant was used (n = 8). Group 1 used physiologic normal saline, group 2 used 6% NaOCl, and group 3 used 10 wt% urushiol solution. After canal irrigation, each sample was collected by the sequential placement of 2 sterile paper points (ProTaper NEXT paper points, size X3, Dentsply). Ten-fold serial dilutions on each vials, and 100 µL were cultured on a BHI agar plate for 8 hours, and colony forming unit (CFU) analysis was done. The data were statistically analyzed using Kruskal-Wallis and Mann-whitney U tests.

Results

Saline group exhibited no difference in the CFU counts with control group, while NaOCl and urushiol groups showed significantly less CFU counts than saline and control groups (p < 0.05).

Conclusions

The result of this study suggests 10% urushiol and 6% NaOCl solution had powerful antibacterial activity against E. faecalis when they were used as root canal irrigants.

Citations

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  • Antimicrobial efficacy of natural-based endodontic solutions: a systematic review with a network meta-analysis
    Danilo Cassiano FERRAZ, Anahi de Paula MELO, Felipe de Souza MATOS, Luiz Renato PARANHOS, Camilla Christian Gomes MOURA, Cauane BLUMENBERG, Juliane Maria GUERREIRO-TANOMARU, Mário TANOMARU-FILHO
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    Nur Asmah, Dewi Fatma Suniarti, Endang Winiati Bachtiar, Dewi Angraini Margono, Basri A. Gani
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Comparative assessment of antibacterial activity of different glass ionomer cements on cariogenic bacteria
Rahul Gaybarao Naik, Arun Suresh Dodamani, Mahesh Ravindra Khairnar, Harish Chaitram Jadhav, Manjiri Abhay Deshmukh
Restor Dent Endod 2016;41(4):278-282.   Published online September 20, 2016
DOI: https://doi.org/10.5395/rde.2016.41.4.278
AbstractAbstract PDFPubReaderePub
Objectives

Glass ionomer cements (GICs), which are biocompatible and adhesive to the tooth surface, are widely used nowadays for tooth restoration. They inhibit the demineralization and promote the remineralization of the tooth structure adjacent to the restoration, as well as interfere with bacterial growth. Hence, the present study was conducted to assess and compare the antimicrobial activity of three commercially available GICs against two cariogenic bacteria.

Materials and Methods

An agar plate diffusion test was used for evaluating the antimicrobial effect of three different GICs (Fuji IX, Ketac Molar, and d-tech) on Streptococcus mutans (S. mutans) and Lactobacillus acidophilus (L. acidophilus). Thirty plates were prepared and divided into two groups. The first group was inoculated with S. mutans, and the second group was inoculated with L. acidophilus. These plates were then incubated at 37℃ for 24 hours. Zones of bacterial growth inhibition that formed around each well were recorded in millimeters (mm).

Results

The zones of inhibition for Fuji IX, Ketac Molar, and d-tech on S. mutans were found to be 10.84 ± 0.22 mm, 10.23 ± 0.15 mm, and 15.65 ± 0.31 mm, respectively, whereas those for L. acidophilus were found to be 10.43 ± 0.12 mm, 10.16 ± 0.11 mm, and 15.57 ± 0.13 mm, respectively.

Conclusions

D-tech cement performed better in terms of the zone of bacterial inhibition against the two test bacteria, than the other two tested glass ionomers.

Citations

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    RV Aparajitha, PSenthamil Selvan, AShafie Ahamed, S Bhavani, V Nagarajan
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    Yuyao Huang, Bingqing Song, Xuedong Zhou, Hui Chen, Haohao Wang, Lei Cheng
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    Shuhei Hoshika, Shihchun Ting, Zubaer Ahmed, Fei Chen, Yu Toida, Norihito Sakaguchi, Bart Van Meerbeek, Hidehiko Sano, Sharanbir K. Sidhu
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    Saeed Hesaraki, Mohammad Karimi, Nader Nezafati
    Materials Science and Engineering: C.2020; 109: 110592.     CrossRef
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Antibacterial properties of composite resins incorporating silver and zinc oxide nanoparticles on Streptococcus mutans and Lactobacillus
Shahin Kasraei, Lida Sami, Sareh Hendi, Mohammad-Yousef AliKhani, Loghman Rezaei-Soufi, Zahra Khamverdi
Restor Dent Endod 2014;39(2):109-114.   Published online March 21, 2014
DOI: https://doi.org/10.5395/rde.2014.39.2.109
AbstractAbstract PDFPubReaderePub
Objectives

Recurrent caries was partly ascribed to lack of antibacterial properties in composite resin. Silver and zinc nanoparticles are considered to be broad-spectrum antibacterial agents. The aim of the present study was to evaluate the antibacterial properties of composite resins containing 1% silver and zinc-oxide nanoparticles on Streptococcus mutans and Lactobacillus.

Materials and Methods

Ninety discoid tablets containing 0%, 1% nano-silver and 1% nano zinc-oxide particles were prepared from flowable composite resin (n = 30). The antibacterial properties of composite resin discs were evaluated by direct contact test. Diluted solutions of Streptococcus mutans (PTCC 1683) and Lactobacillus (PTCC 1643) were prepared. 0.01 mL of each bacterial species was separately placed on the discs. The discs were transferred to liquid culture media and were incubated at 37℃ for 8 hr. 0.01 mL of each solution was cultured on blood agar and the colonies were counted. Data was analyzed with Kruskall-Wallis and Mann-Whitney U tests.

Results

Composites containing nano zinc-oxide particles or silver nanoparticles exhibited higher antibacterial activity against Streptococcus mutans and Lactobacillus compared to the control group (p < 0.05). The effect of zinc-oxide on Streptococcus mutans was significantly higher than that of silver (p < 0.05). There were no significant differences in the antibacterial activity against Lactobacillus between composites containing silver nanoparticles and those containing zinc-oxide nanoparticles.

Conclusions

Composite resins containing silver or zinc-oxide nanoparticles exhibited antibacterial activity against Streptococcus mutans and Lactobacillus.

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Antibacterial effect of self-etching adhesive systems on Streptococcus mutans
Seung-Ryong Kim, Dong-Hoon Shin
Restor Dent Endod 2014;39(1):32-38.   Published online January 20, 2014
DOI: https://doi.org/10.5395/rde.2014.39.1.32
AbstractAbstract PDFPubReaderePub
Objectives

In this study, we evaluated the antibacterial activity of self-etching adhesive systems against Streptococcus mutans using the agar diffusion method.

Materials and Methods

Three 2-step systems, Clearfil SE Bond (SE, Kuraray), Contax (CT, DMG), and Unifil Bond (UnB, GC), and three 1-step systems, Easy Bond (EB, 3M ESPE), U-Bond (UB, Vericom), and All Bond SE (AB, BISCO) were used. 0.12% chlorhexidine (CHX, Bukwang) and 37% phosphoric acid gel (PA, Vericom) were used as positive controls.

Results

The antibacterial activity of CHX and PA was stronger than that of the other groups, except SE. After light activation, the inhibition zone was reduced in the case of all 2-step systems except CT. However, all 1-step systems did not exhibit any inhibition zone upon the light activation.

Conclusions

SE may be better than CT or UnB among the 2-step systems with respect to antibacterial activity, however, 1-step systems do not exhibit any antibacterial activity after light curing.

Citations

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Inhibition of Streptococcus mutans biofilm formation on composite resins containing ursolic acid
Soohyeon Kim, Minju Song, Byoung-Duck Roh, Sung-Ho Park, Jeong-Won Park
Restor Dent Endod 2013;38(2):65-72.   Published online May 28, 2013
DOI: https://doi.org/10.5395/rde.2013.38.2.65
AbstractAbstract PDFPubReaderePub
Objectives

To evaluate the inhibitory effect of ursolic acid (UA)-containing composites on Streptococcus mutans (S. mutans) biofilm.

Materials and Methods

Composite resins with five different concentrations (0.04, 0.1, 0.2, 0.5, and 1.0 wt%) of UA (U6753, Sigma Aldrich) were prepared, and their flexural strengths were measured according to ISO 4049. To evaluate the effect of carbohydrate source on biofilm formation, either glucose or sucrose was used as a nutrient source, and to investigate the effect of saliva treatment, the specimen were treated with either unstimulated whole saliva or phosphate-buffered saline (PBS). For biofilm assay, composite disks were transferred to S. mutans suspension and incubated for 24 hr. Afterwards, the specimens were rinsed with PBS and sonicated. The colony forming units (CFU) of the disrupted biofilm cultures were enumerated. For growth inhibition test, the composites were placed on a polystyrene well cluster, and S. mutans suspension was inoculated. The optical density at 600 nm (OD600) was recorded by Infinite F200 pro apparatus (TECAN). One-way ANOVA and two-way ANOVA followed by Bonferroni correction were used for the data analyses.

Results

The flexural strength values did not show significant difference at any concentration (p > 0.01). In biofilm assay, the CFU score decreased as the concentration of UA increased. The influence of saliva pretreatment was conflicting. The sucrose groups exhibited higher CFU score than glucose group (p < 0.05). In bacterial growth inhibition test, all experimental groups containing UA resulted in complete inhibition.

Conclusions

Within the limitations of the experiments, UA included in the composite showed inhibitory effect on S. mutans biofilm formation and growth.

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    Zhejun Wang, Ya Shen, Markus Haapasalo
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    W. Krzyściak, A. Jurczak, D. Kościelniak, B. Bystrowska, A. Skalniak
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Inhibitory effect on Streptococcus mutans and mechanical properties of the chitosan containing composite resin
Ji-Sun Kim, Dong-Hoon Shin
Restor Dent Endod 2013;38(1):36-42.   Published online February 26, 2013
DOI: https://doi.org/10.5395/rde.2013.38.1.36
AbstractAbstract PDFPubReaderePub
Objectives

This study evaluated the antibacterial effect and mechanical properties of composite resins (LCR, MCR, HCR) incorporating chitosan with three different molecular weights (L, Low; M, Medium; H, High).

Materials and Methods

Streptococcus (S). mutans 100 mL and each chitosan powder were inoculated in sterilized 10 mL Brain-Heart Infusion (BHI) solution, and was centrifuged for 12 hr. Absorbance of the supernatent was measured at OD660 to estimate the antibacterial activities of chitosan. After S. mutans was inoculated in the disc shaped chitosan-containing composite resins, the disc was cleansed with BHI and diluted with serial dilution method. S. mutans was spread on Mitis-salivarius bacitracin agar. After then, colony forming unit (CFU) was measured to verify the inhibitory effect on S. mutans biofilm. To ascertain the effect on the mechanical properties of composite resin, 3-point bending and Vickers hardness tests were done after 1 and 3 wk water storage, respectively. Using 2-way analysis of variance (ANOVA) and Scheffe test, statistical analysis was done with 95% significance level.

Results

All chitosan powder showed inhibition effect against S. mutans. CFU number in chitosan-containing composite resins was smaller than that of control resin without chitosan. The chitosan containing composite resins did not show any significant difference in flexural strength and Vickers hardness in comparison with the control resin. However, the composite resin, MCR showed a slightly decreased flexural strength and the maximum load than those of control and the other composite resins HCR and LCR.

Conclusions

LCR and HCR would be recommended as a feasible antibacterial restorative due to its antibacterial nature and mechanical properties.

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Original Articles
Comparison of Antibacterial effect of Listerine® with Various root canal irrigants
Young Hun Kim, Min-Kyung Kang, Eun-Kyoung Choi, So-Young Yang, Inseok Yang, In-Chol Kang, Yun-Chan Hwang, In-Nam Hwang, Won-Mann Oh
J Korean Acad Conserv Dent 2009;34(6):500-507.   Published online November 30, 2009
DOI: https://doi.org/10.5395/JKACD.2009.34.6.500
AbstractAbstract PDFPubReaderePub

The purpose of this study is to compare the antibacterial effect of Listerine® on two microorganisms (P. gingivalis and E. faecalis) with various root canal irrigants (NaOCl, CHX, EDTA) and to identify possibility of using Listerine® as a root canal irrigant. Porphyromonas gingivalis ATCC 3327 and Enterococcus faecalis ATCC 29212 were used in this experiment. For the test irrigants, 0.5%, 1%, 2.5%, 5.25% NaOCl, 0.1%, 0.2%, 1%, 2% CHX, 0.5M EDTA (18.6% EDTA) and Listerine® were prepared. Distiled water was used as control. Two methods-1) Comparison of turbidity in broth and 2) Agar diffusion test-were used to determine the extent of antibacterial effect of Listerine® and to compare it with that of NaOCl, CHX, and EDTA. All solutions tested were effective against two bacterial strains compared with control (p<0.001). Any concentration of NaOCl, CHX, and EDTA showed similarly high effectiveness against all bacterial strains. In all experiment, Listerine® showed significantly low antibacterial effect compared with the other root canal irrigants (p<0.05).

In conclusion, the results reflect remarkably low antibacterial effect of Listerine® as compared with root canal irrigants in general so it is not suitable for the root canal irrigant.

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The effect of MTAD as a final root canal irrigants on the coronal bacterial leakage of obturated root canals
Tae Woo Kim, Seok Woo Chang, Dong Sung Park
J Korean Acad Conserv Dent 2008;33(4):397-404.   Published online July 31, 2008
DOI: https://doi.org/10.5395/JKACD.2008.33.4.397
AbstractAbstract PDFPubReaderePub

The purpose of this study was to evaluate the effects of MTAD, EDTA and sodium hypochlorite(NaOCl) as final irrigants on coronal leakage resistance to Enterococcus faecalis. Forty extracted human maxillary molars were used in this experiment. The teeth were randomly divided into positive control group (Group 1; n = 5), negative control group (Group 2; n = 5) and three experimental groups (n = 30). In Group 3 (n = 10), the root canals were irrigated with sodium hypochlorite. In Group 4 (n = 10) and 5 (n = 10), the root canals were irrigated with sodium hypochlorite and rinsed with EDTA and MTAD, respectively. The teeth in each group were cleaned and shaped to #40 profile with .04 taper, and obturated with gutta-percha and AH-26 root canal sealer. The coronal portion of each tooth was placed in contact with inoculum of Enterococcus faecalis in Brain Heart Infusion (BHI) culture media. Each root tip was placed in a vial containing sterile culture media. The vials were placed in anaerobic chamber and observed everyday for turbidity for 180 days. Statistical analysis was performed using Fisher's Exact Test. After 180 days, Group 3, 4, and 5 showed 7, 4 and 5 leaking samples respectively. The differences in leakage resistance were not statistically significant among Group 3, 4 and 5.

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Isolation and identification of bacteria from the root canal of the teeth diagnosed as the acute pulpitis and acute periapical abscess
Yeon-Jae Lee, Mi-Kwang Kim, Ho-Keel Hwang, Joong-Ki Kook
J Korean Acad Conserv Dent 2005;30(5):409-422.   Published online September 30, 2005
DOI: https://doi.org/10.5395/JKACD.2005.30.5.409
AbstractAbstract PDFPubReaderePub

The aim of this study was to identify the bacteria isolated from acute endodontic lesions by cell culture and 16S rDNA sequencing. The necrotic pulpal tissue was collected from 17 infected root canals, which were diagnosed as being either an acute pulpitis or acute periapical abscess. Samples were collected aseptically from the infected pulpal tissue of the infected root canals using a barbed broach and a paper point. The cut barbed broaches and paper points were transferred to an eppendorf tube containing 500 ul of 1 X PBS. The sample solution was briefly mixed and plated onto a BHI-agar plate containing 5% sheep blood. The agar plates were incubated in a 37℃ anaerobic chamber for 7 days. The bacteria growing on the agar plate were identified by 16S rRNA coding gene (rDNA) cloning and sequencing at the species level. Among the 71 colonies grown on the agar plates, 56 strains survived and were identified. In dental caries involving the root canals, Streptococcus spp. were mainly isolated. Actinomyces, Clostridia, Bacteroides and Fusobacteria were isolated in the periapical lesion without dental caries. Interestingly, two new Actinomyces spp. (ChDC B639 and ChDC B631) were isolated in this study. These results showed that there was diversity among the species in endodontic lesions. This suggests that an endodontic infection is a mixed infection with a polymicrobial etiology. These results may offer the bacterial strains for pathogenesis studies related to an endodontic infection.

Citations

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  • Microorganism penetration in dentinal tubules of instrumented and retreated root canal walls.In vitroSEM study
    Saad Al-Nazhan, Alaa Al-Sulaiman, Fellwa Al-Rasheed, Fatimah Alnajjar, Bander Al-Abdulwahab, Abdulhakeem Al-Badah
    Restorative Dentistry & Endodontics.2014; 39(4): 258.     CrossRef
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    Sang-Min Lim, Tae-Kwon Lee, Eun-Jeong Kim, Jun-Hong Park, Yoon Lee, Kwang-Shik Bae, Kee-Yeon Kum
    Journal of Korean Academy of Conservative Dentistry.2011; 36(6): 498.     CrossRef
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Bacteriologic in vitro coronal leakage study of before and after post space preparation
Hyo-An Lee, Eui-Seong Kim
J Korean Acad Conserv Dent 2005;30(1):16-21.   Published online January 31, 2005
DOI: https://doi.org/10.5395/JKACD.2005.30.1.016
AbstractAbstract PDFPubReaderePub

The purpose of present study was to compare the speed of coronal leakage before and after post space preparation using Streptococcus mutans.

Forty straight extracted human teeth were selected. The crowns were removed to a uniform remaining root length 14 mm. Canals were enlarged by 06 taper Profiles® to a size #40 as a master apical file. And these were filled with gutta percha point and Tubuliseal® sealer, using continuous wave technique. Groupings are as follows.

Group 1 - These teeth were obturated without sealer.

Group 2 - These teeth were obturated and covered the surface of the root completely with sticky wax.

Group 3 - These teeth were obturated.

Group 4 - These teeth were obturated and prepared for post space remaining 5 mm of gutta percha.

The teeth were suspended in plastic tubes. The upper chamber received the bacterial suspension everyday to simulate clinical situation. The lower chamber consisted of BHI added Andrade's indicator.

All roots in the positive control group (Group 1) turned yellow within 24 h and those of negative control group (Group 2) remained red throughout the experimental period (70 days). The samples of group 3 were contaminated within an average of 27.2 days. The samples of group 4 were contaminated within an average of 15.7 days, ranging from 9 to 22 days.

There was significant difference between group 3 and group 4 statistically (p < 0.05).

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Antibacterial effect of polyphosphate on endodontopathic bacteria
Jeong-Hee Shin, Sang-Jin Park, Gi-Woon Choi
J Korean Acad Conserv Dent 2003;28(6):435-448.   Published online November 30, 2003
DOI: https://doi.org/10.5395/JKACD.2003.28.6.435
AbstractAbstract PDFPubReaderePub

This study was performed to observe the antibacterial effect of polyphosphate (polyP) with various chain lengths (P3~P75) on virulent, invasive strains of P. gingivalis A7A1-28 and W50, and multidrug resistant E. faecalis ATCC29212. P. gingivalis strains were grown in brain-heart infusion broth (BHI) containing hemin and vitamin K with or without polyP. PolyP was added at the very beginning of the culture or during the exponential growth phase of the culture. Inhibition of the growth of P. gingivalis was determined by measuring the absorbancy at 540nm of the grown cells. Viable cell counts of the culture and release of intracellular nucleotide from P. gingivalis were measured. E. faecalis was grown in plain BHI with antibiotics alone or in combination with polyP(calgon; 0.1~1.0%) and the bacterial absorbancy was measured.

The overall results suggest that polyP has a strong antibacterial effect on the growth of the virulent strains of P. gingivalis and the antibacterial activity of polyP seems largely bactericidal, accompanying bacteriolysis in which chelation phenomenon is not involved. Although polyP does not exert antibacterial activity against E. faecalis, it appears to increase antibacterial effect of erythromycin and tetracycline on the bacterium. Therefore, polyP alone or in combination with antibiotics may be developed as a candidate for the agent controlling oral infections including endodontic infection.

Citations

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  • Minocycline-loaded calcium polyphosphate glass microspheres as a potential drug-delivery agent for the treatment of periodontitis
    Iain Gibson, Arash Momeni, Mark Filiaggi
    Journal of Applied Biomaterials & Functional Materials.2019;[Epub]     CrossRef
  • Comparison of Antibacterial effect of Listerine® with Various root canal irrigants
    Young Hun Kim, Min-Kyung Kang, Eun-Kyoung Choi, So-Young Yang, Inseok Yang, In-Chol Kang, Yun-Chan Hwang, In-Nam Hwang, Won-Mann Oh
    Journal of Korean Academy of Conservative Dentistry.2009; 34(6): 500.     CrossRef
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A study of ionic dissociation on various calcium hydroxide pastes using molecular sieving model
Kyoung-Sun Lee, Seung-Jong Lee
J Korean Acad Conserv Dent 2002;27(6):632-643.   Published online November 30, 2002
DOI: https://doi.org/10.5395/JKACD.2002.27.6.632
AbstractAbstract PDFPubReaderePub

The purpose of this study was two-fold. First was to evaluate whether the molecular sieving model was appropriate for ionic dissociation experiment. Second was to compare the dissociation of calcium and hydroxyl ions from five types of calcium hydroxide pastes (Pure calcium hydroxide paste, DT temporary dressing®, Metapaste®, Chidopex®, Metapex®) in three vehicles (aqueous, viscous and oily) and the antibacterial effect.

Each calcium hydroxide pastes was placed into 0.65ml tube with cap and then 15% polyacrylamide gel was placed onto calcium hydroxide pastes. After the gel was hardened, the tubes were filled with tridistilled water (pH 7.14) and closed with cap. The tubes were stored in 37℃, 100% incubator. The pH reading and the concentration of calcium ions were taken at 1, 4, 7, 10, and 14 days. The brain heart infusion agar plates with S. mutans and A. actinomycetemcomitans were used for antibacterial activity test. Middle of agar plate was filled with the calcium hydroxide pastes. The plates were incubated at 37℃ and observations were made to detect the zones of inhibition. These data were evaluated statistically by use of the analysis of variance and duncan test.

The results were as follows.

1. In fresh mixing state, the pH of five types of calcium hydroxide pastes were measured between 12.5 and 12.8.

2. The pH was increased in all five types of calcium hydroxide pastes compared with control group. In 14 days, Pure calcium hydroxide paste (11.45) and DT temporary dressing® (11.33) showed highest pH, followed by Metapaste® (9.49), Chidopex® (8.37) and Metapex® (7.59).

3. Calcium was higher in all five types of calcium hydroxide pastes compared with control group. In 14 days, Pure calcium hydroxide paste (137.29 mg%) and DT temporary dressing® (124.6 mg%) showed highest value, followed by Metapaste® (116.74 mg%), Chidopex® (111.84 mg%) and Metapex® (60.22 mg%).

4. The zones of bacterial inhibition were seen around all five types of calcium hydroxide pastes. Chidopex® and Metapex® groups which include iodoform were observed significantly larger zone of inhibition in A. actinomycetemcomitans compared with the other calcium hydroxide groups (p<0.05). However, Metapex® showed the least antibacterial effect on S. mutans compared with other groups (p<0.05).

The molecular sieving model was found to be acceptable in dissociation experiment of hydroxyl and calcium ions when compared with the previous tooth model study. But this model was not appropriate for the antibacterial test.

Citations

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  • A Comparison of the irrigation systems in calcium hydroxide removal
    Jae-Seung Eun, Se-Hee Park, Kyung-Mo Cho, Jin-Woo Kim
    Journal of Korean Academy of Conservative Dentistry.2009; 34(6): 508.     CrossRef
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Comparison of ultrasonic and sonic root end preparations using anaerobic bacterial leakage model
Nak-Yeon Cho, Dong-Sung Park, Hyeon-Mee Yoo, Tae-Seok Oh
J Korean Acad Conserv Dent 2002;27(5):530-534.   Published online September 30, 2002
DOI: https://doi.org/10.5395/JKACD.2002.27.5.530
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