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Research Articles
Single-session associative protocol for dentin hypersensitivity management: a 1-year randomized, blinded clinical study
Thayna Carolina Zeni, Poliana Maria de Faveri Cardoso, Rafael da Silva Vanolli, Márcio José Mendonça, Julio Katuhide Ueda, Veridiana Camilotti
Restor Dent Endod 2024;49(2):e15.   Published online March 20, 2024
DOI: https://doi.org/10.5395/rde.2024.49.e15
AbstractAbstract PDFPubReaderePub
Objectives

This study aimed to establish a single-session associative protocol for non-restorative management of dentin hypersensitivity (DH).

Materials and Methods

Twenty-four individuals with DH and a minimum sensitivity level of 4 on the visual analog scale (VAS) were selected. The study was conducted in a split-mouth design, with each participant (n = 20) having at least 1 affected tooth in all quadrants. The management protocols consisted of control group: universal adhesive, Neural Desensitizing Protocol group: 5% potassium nitrate, Mixed Desensitizing Protocol (PAM) group: 5% sodium fluoride and 5% potassium nitrate, Remineralizing Desensitizing Protocol (PDR) group: surface-partially reacted glass technology photopolymerizable varnish. Evaluations were performed immediately after application, at 1 week, 1 month, 2 months, and 12 months using the VAS sensitivity test.

Results

The scores were subjected to statistical analysis using the Friedman test (p < 0.05), Durbin-Conover test (p < 0.05), and Wilcoxon test (p < 0.05). At the 12-month evaluation, all groups showed statistically significant differences compared to the initial assessment. For the evaluation after 12 months, there was a statistically significant difference between the PAM group, the control group, and the PDR group.

Conclusions

It can be concluded that all groups were effective in controlling DH, but there were significant results in the control group and PDR group. The clinical relevance of this study is to demonstrate that the application of single-session desensitizing protocols can be effective in controlling DH for up to 12 months.

Trial Registration

Brazilian Clinical Trials Registry Identifier: RBR-4r63d7s

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Effects of eye dominance on shade matching and color perception among the dentist population
Pattnaik Kalyani, Kannan Subiksha, Amit Jena, Govind Shashirekha, Saumyakanta Mohanty, Gaurav Sharma
Restor Dent Endod 2023;48(4):e40.   Published online November 9, 2023
DOI: https://doi.org/10.5395/rde.2023.48.e40
AbstractAbstract PDFPubReaderePub
Objectives

The purpose of this study was to evaluate the influence of eye dominance on color perception, and shade matching.

Materials and Methods

A total of 104 participants were selected for the study. There were 3 groups: Group I: 3rd and 4th year dental students and interns (n = 40); Group II: postgraduates (n = 34); Group III: senior residents and faculty members (≥ 6 years of clinical experience) (n = 30). All participants were evaluated for congenital color blindness with Ishihara plates, their dominant eye with Mile's test, and their color perception with the Farnsworth-Munsell 100 hue test. The shade guide test was used for shade matching with a second corresponding set of Vitapan classical shade guides.

Results

The results of Mile’s test revealed that 60.6% were right-eye dominant and 39.4% were left-eye dominant. There was a statistically significant difference among all participants between the dominant eye and the non-dominant eye in shade matching.

Conclusions

The dominant eye has a positive effect on shade matching and the ability to match shades becomes better with an increase in clinical experience.

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Incidence of interappointment emergencies in multiple-visit root canal treatments performed with or without intracanal medicament by undergraduate students
Annemarie Baaij, Corine Mirjam Visscher, Manon Jansen, Ahmet Rifat Özok
Restor Dent Endod 2023;48(3):e31.   Published online August 14, 2023
DOI: https://doi.org/10.5395/rde.2023.48.e31
AbstractAbstract PDFPubReaderePub
Objectives

This retrospective cohort study examined the incidence of interappointment emergencies during multiple-visit molar root canal treatments conducted by undergraduate students. Treatments performed without the use of intracanal medicament were compared to treatments that incorporated calcium hydroxide as an intracanal medicament.

Materials and Methods

Interappointment emergencies, defined as instances of pain or swelling that required the patient to make an unscheduled follow-up visit, were recorded for up to 2 months after the intervention. To avoid the influence of obturation on the observed incidence of emergency visits, only unscheduled visits occurring between the start and end of the root canal treatment were included.

Results

Of the 719 patients included in this study, 77 (10.7%) were recorded as experiencing interappointment emergencies. Of these emergencies, 62% occurred within 2 weeks following the most recent intervention. In the group of patients who did not receive intracanal medicament, the incidence of interappointment emergencies was 11.9% (46 of 385 patients). In comparison, this rate was 9.3% (31 of 334 patients) among those who received calcium hydroxide as an intracanal medicament (odds ratio, 1.33; 95% confidence interval, 0.82–2.15; p = 0.249).

Conclusions

Interappointment emergencies may arise at any point during root canal treatment, but they most commonly occur within the first 2 weeks following intervention. The omission of intracanal medicament in multiple-visit molar root canal treatments, performed by undergraduate students, did not significantly increase the incidence of these emergencies.

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A 3-year retrospective study of clinical durability of bulk-filled resin composite restorations
Muhittin Ugurlu, Fatmanur Sari
Restor Dent Endod 2022;47(1):e5.   Published online December 30, 2021
DOI: https://doi.org/10.5395/rde.2022.47.e5
AbstractAbstract PDFPubReaderePub
Objectives

This study aimed to assess the clinical longevity of a bulk-fill resin composite in Class II restorations for 3-year.

Materials and Methods

Patient record files acquired from the 40 patients who were treated due to needed 2 similar sizes Class II composite restorations were used for this retrospective study. In the experimental cavity, the flowable resin composite SDR was inserted in the dentinal part as a 4 mm intermediate layer. A 2 mm coverage layer with a nano-hybrid resin composite (CeramX) was placed on SDR. The control restoration was performed by an incremental technique of 2 mm using the nano-hybrid resin composite. The restorations were blindly assessed by 2 calibrated examiners using modified United States Public Health Service criteria at baseline and 1, 2, and 3 years. The data were analyzed using non-parametric tests (p = 0.05).

Results

Eighty Class II restorations were evaluated. After 3-years, 4 restorations (5%) failed, 1 SDR + CeramX, and 3 CeramX restorations. The annual failure rate (AFR) of the restorations was 1.7%. The SDR + CeramX group revealed an AFR of 0.8%, and the CeramX group an AFR of 2.5% (p > 0.05). Regarding anatomical form and marginal adaptation, significant alterations were observed in the CeramX group after 3-years (p < 0.05). The changes in the color match were observed in each group over time (p < 0.05).

Conclusions

The use of SDR demonstrated good clinical durability in deep Class II resin composite restorations.

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Microhybrid versus nanofill composite in combination with a three step etch and rinse adhesive in occlusal cavities: five year results
Safa Tuncer, Mustafa Demirci, Evren Öztaş, Neslihan Tekçe, Ömer Uysal
Restor Dent Endod 2017;42(4):253-263.   Published online August 21, 2017
DOI: https://doi.org/10.5395/rde.2017.42.4.253
AbstractAbstract PDFPubReaderePub
Objectives

The aim of the study was to evaluate the 5-year clinical performance of occlusal carious restorations using nanofill and microhybrid composites, in combination with 3-step etch-and-rinse adhesives, in patients who were going to commence orthodontic treatment.

Materials and Methods

A total of 118 restorations for occlusal caries were conducted prior to orthodontic treatment. Occlusal restorations were performed both with Filtek Supreme XT (3M ESPE) and Filtek Z250 (3M ESPE) before beginning orthodontic treatment with fixed orthodontic bands. Restorations were clinically evaluated at baseline and at 1, 2, 3, 4, and 5-year recalls.

Results

None of the microhybrid (Filtek Z250) and nanofill (Filtek Supreme XT) composite restorations was clinically unacceptable with respect to color match, marginal discoloration, wear or loss of anatomical form, recurrent caries, marginal adaptation, or surface texture. A 100% success rate was recorded for both composite materials. There were no statistically significant differences in any of the clinical evaluation criteria between Filtek Z250 and Filtek Supreme XT restorations for each evaluation period.

Conclusions

The composite restorations showed promising clinical results relating to color matching, marginal discoloration, wear or loss of anatomical form, recurrent caries, marginal adaptation, and surface texture at the end of the 5-year evaluation period.

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Review Article
Current perspectives of bio-ceramic technology in endodontics: calcium enriched mixture cement - review of its composition, properties and applications
Shivani Utneja, Ruchika Roongta Nawal, Sangeeta Talwar, Mahesh Verma
Restor Dent Endod 2015;40(1):1-13.   Published online November 3, 2014
DOI: https://doi.org/10.5395/rde.2015.40.1.1
AbstractAbstract PDFPubReaderePub

Advancements in bio-ceramic technology has revolutionised endodontic material science by enhancing the treatment outcome for patients. This class of dental materials conciliates excellent biocompatibility with high osseoconductivity that render them ideal for endodontic care. Few recently introduced bio-ceramic materials have shown considerable clinical success over their early generations in terms of good handling characteristics. Calcium enriched mixture (CEM) cement, Endosequence sealer, and root repair materials, Biodentine and BioAggregate are the new classes of bio-ceramic materials. The aim of this literature review is to present investigations regarding properties and applications of CEM cement in endodontics. A review of the existing literature was performed by using electronic and hand searching methods for CEM cement from January 2006 to December 2013. CEM cement has a different chemical composition from that of mineral trioxide aggregate (MTA) but has similar clinical applications. It combines the biocompatibility of MTA with more efficient characteristics, such as significantly shorter setting time, good handling characteristics, no staining of tooth and effective seal against bacterial leakage.

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Research Article
Correlation between clinical clerkship achievement and objective structured clinical examination (OSCE) scores of graduating dental students on conservative dentistry
Jae-Beum Bang, Kyoung-Kyu Choi
Restor Dent Endod 2013;38(2):79-84.   Published online May 28, 2013
DOI: https://doi.org/10.5395/rde.2013.38.2.79
AbstractAbstract PDFPubReaderePub
Objectives

This study aimed to investigate the effect of clinical clerkship-associated achievements, such as performance of procedures at the student clinic, observation, and attitude towards a clerkship, on the objective structured clinical examination (OSCE) scores of dental students graduating in restorative dentistry.

Materials and Methods

The OSCEs consisted of two stations designed to assess students' clinical skills regarding cavity preparation for a class II gold inlay and a class IV composite restoration. The clerkship achievements, consisting of the number of student clinical procedures performed, observation-related OSCE, and scores of their attitudes towards a conservative dentistry clerkship, were assessed. Correlation and multiple regression analyses were conducted.

Results

The correlation coefficient between the OSCE scores for cavity preparation for a class II gold restoration and clerkship attitude scores was 0.241 (p < 0.05). Regarding a class IV composite restoration, OSCE scores showed statistically significant correlations with the observation (r = 0.344, p < 0.01) and attitude (r = 0.303, p < 0.01) scores. In a multiple regression analysis, attitudes towards a clerkship (p = 0.033) was associated with the cavity preparation for a class II gold inlay OSCE scores, while the number of procedure observations (p = 0.002) was associated with the class IV composite restoration OSCE scores.

Conclusions

The number of clinical procedures performed by students, which is an important requirement for graduation, showed no correlation with either of the OSCEs scores.

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Clinical Researchs
The effect of clinical performance on the survival estimates of direct restorations
Kyou-Li Kim, Cheol Namgung, Byeong-Hoon Cho
Restor Dent Endod 2013;38(1):11-20.   Published online February 26, 2013
DOI: https://doi.org/10.5395/rde.2013.38.1.11
AbstractAbstract PDFPubReaderePub
Objectives

In most retrospective studies, the clinical performance of restorations had not been considered in survival analysis. This study investigated the effect of including the clinically unacceptable cases according to modified United States Public Health Service (USPHS) criteria into the failed data on the survival analysis of direct restorations as to the longevity and prognostic variables.

Materials and Methods

Nine hundred and sixty-seven direct restorations were evaluated. The data of 204 retreated restorations were collected from the records, and clinical performance of 763 restorations in function was evaluated according to modified USPHS criteria by two observers. The longevity and prognostic variables of the restorations were compared with a factor of involving clinically unacceptable cases into the failures using Kaplan-Meier survival analysis and Cox proportional hazard model.

Results

The median survival times of amalgam, composite resin and glass ionomer were 11.8, 11.0 and 6.8 years, respectively. Glass ionomer showed significantly lower longevity than composite resin and amalgam. When clinically unacceptable restorations were included into the failure, the median survival times of them decreased to 8.9, 9.7 and 6.4 years, respectively.

Conclusions

After considering the clinical performance, composite resin was the only material that showed a difference in the longevity (p < 0.05) and the significantly higher relative risk of student group than professor group disappeared in operator groups. Even in the design of retrospective study, clinical evaluation needs to be included.

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Short-term clinical outcome of intentionally replanted posterior molars
Yong-Hoon Choi
J Korean Acad Conserv Dent 2011;36(1):12-18.   Published online January 31, 2011
DOI: https://doi.org/10.5395/JKACD.2011.36.1.12
AbstractAbstract PDFPubReaderePub
Objectives

This retrospective study evaluated the therapeutic effects of the intentional replantation (IR) procedure performed on the maxillary and mandibular molars of 35 patients.

Materials and Methods

For the subjects, IR was performed due to difficulties in anatomically accessing the lesions and/or close proximity to the thick cortical bone, inferior alveolar nerve, or maxillary sinus, which rendered the ordinary periradicular surgery impossible. The patients' progress was followed for a year and up to 2 years and 4 months. The success of the procedure was evaluated in terms of clinical and radiographic success (%).

Results

The results revealed the following: (a) 1 case (3%) of failed tooth extraction during IR; (b) 2 cases (6%) of extraction due to periodontal diseases and inflammatory root resorption; (c) 3 cases (9%) of normally functioning teeth in the oral cavity with minor mobility and apical root resorption, and; (d) 29 cases (82%) of normally functioning teeth without obvious problems.

Conclusions

IR was confirmed to be a reliably repeatable, predictable treatment option for those who cannot receive conventional periradicular surgery because of anatomic limitations or patient factors.

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Prognostic factors influencing clinical outcome of nonsurgical endodontic treatment
Seonah Kim
J Korean Acad Conserv Dent 2010;35(6):436-444.   Published online November 30, 2010
DOI: https://doi.org/10.5395/JKACD.2010.35.6.436
AbstractAbstract PDFPubReaderePub
Objectives

This study aimed to assess prospectively the clinical outcome of nonsurgical endodontic treatment and identify patient- and tooth-related factors, rather than treatment-related factors, that were the best predictors of this outcome.

Materials and Methods

The inception cohort comprised 441 teeth (320 patients) and 175 teeth (123 patients) were followed up for 1-2 years. Age, gender, presence of medical disease, number of canals, previous endodontic treatment, presence of sensitivity and pain, pulp vitality, swelling or sinus tract of pulpal origin on the gingiva, periapical radiolucency and tendency of unilateral bite on the affected tooth were recorded at treatment start.

Results

The outcome was classified on the basis of periapical radiolucency as healed or non healed. The overall healed rate in these cases, including nonsurgical retreatment, was 81.1%. Four tooth-related factors had a negative impact in the bivariate analysis: previous endodontic treatment, necrotic pulp, preoperative gingival swelling or sinus tract of pulpal origin, and preoperative periapical radiolucency. Stepwise logistic regression analysis including patient-, tooth-related factors and level of the root canal filling as a treatment-related factor showed that preoperative gingival lesion (odds ratio [OR]: 4.4; p = 0.005), preoperative periapical radiolucency (OR: 3.6; p = 0.011), and ≤ 1-2 mm under root filling length (OR: 9.6; p = 0.012) were significant predictors of failure.

Conclusions

A preoperative gingival lesion of pulpal origin can influence the outcome of nonsurgical endodontic treatment in addition to preoperative periapical radiolucency.

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Basic Researchs
A clinical evaluation of safety of an office bleaching gel containing 30% hydrogen peroxide
Sin-Young Kim, Je-Uk Park, Chang-Hyen Kim, Sung-Eun Yang
J Korean Acad Conserv Dent 2010;35(3):198-210.   Published online May 31, 2010
DOI: https://doi.org/10.5395/JKACD.2010.35.3.198
AbstractAbstract PDFPubReaderePub

This study evaluated the safety of an office bleaching gel (RemeWhite, Remedent Inc., Deurle, Belgium) containing 30% hydrogen peroxide. 37 volunteers were recieved office bleaching with the RemeWhite for 3 times at one visit, total 2 visits. As control group, the same gel in which hydrogen peroxide was not included was applied to 34 volunteers with the same protocol.

There was no difference between experimental group and control group using electric pulp test. In the result of gingival inflammation index and tooth sensitivity test, there was mild pain response in experimental group but it disappeared as time went by. Therefore, safety of the office bleaching gel containing 30% hydrogen peroxide was confirmed.

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A clinical evaluation of efficacy of an office bleaching gel containing 30% hydrogen peroxide
Sin-Young Kim, Je-Uk Park, Chang-Hyen Kim, Sung-Eun Yang
J Korean Acad Conserv Dent 2010;35(1):40-50.   Published online January 31, 2010
DOI: https://doi.org/10.5395/JKACD.2010.35.1.040
AbstractAbstract PDFPubReaderePub

This study evaluated the efficacy of an office bleaching gel (RemeWhite, Remedent Inc., Deurle, Belgium) containing 30% hydrogen peroxide. 31 volunteers were recieved office bleaching with the RemeWhite for 3 times at one visit, total 2 visits. As control group, the same gel in which hydrogen peroxide was not included was applied to 31 volunteers with the same protocol. The shade change (ΔE*, color difference) of 12 anterior teeth was measured using Colorimerter and Vitapan classical shade guide. The shade change of overall teeth in the experimental group was significantly greater than that in the control group which was measured using Colorimeter. There was also a significant difference between baseline and 14 weeks or 26 weeks though color rebounding phenomenon occurred as time went by. Small shade change difference can be measured accurately using Colorimeter than using Vitapan classical shade guide.

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Original Articles
Clinical study of shade improvement and safety of polymer-based pen type BlancTic Forte whitening agent containing 8.3% Carbamide peroxide
Jin-Kyung Lee, Sun-Hong Min, Sung-Tae Hong, So-Ram Oh, Shin-Hye Chung, Young-Hye Hwang, Sung-Yeop You, Kwang-Shik Bae, Seung-Ho Baek, Woo-Cheol Lee, Won-Jun Son, Kee-Yeon Kum
J Korean Acad Conserv Dent 2009;34(2):154-161.   Published online March 31, 2009
DOI: https://doi.org/10.5395/JKACD.2009.34.2.154
AbstractAbstract PDFPubReaderePub

This clinical study evaluated the whitening effect and safety of polymer based-pen type BlancTis Forte (NIBEC) containing 8.3% carbamide peroxide. Twenty volunteers used the BlancTis Forte whitening agent for 2 hours twice a day for 4 weeks. As a control, Whitening Effect Pen (LG) containing 3% hydrogen peroxide was used by 20 volunteers using the same protocol. The change in shade (ΔE*, color difference) was measured using Shadepilot™ (DeguDent) before, during, and after bleaching (2 weeks, 4 weeks, and post-bleaching 4 weeks). A clinical examination for any side effects (tooth hypersensitivity or soft tissue complications) was also performed at each check-up. The following results were obtained.

1. Both the experimental and control groups displayed a noticeable change in shade (ΔE) of over 2. No significant differences were found between the two groups (p > 0.05), implying that the two agents have a similar whitening effect.

2. The whitening effect was mainly due to changes in a and b values rather than in L value (brightness). The experimental group showed a significantly higher change in b value, thus yellow shade, than the control (p < 0.05).

3. None of the participants complained of tooth hypersensitivity or soft tissue complications, confirming the safety of both whitening agents.

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Prospective clinical evaluation of three different bonding systems in class V resin restorations with or without mechanical retention
Kyung-Wook Lee, Sae-Joon Choung, Young-Chul Han, Ho-Hyun Son, Chung-Moon Um, Myoung-Hwan Oh, Byeong-Hoon Cho
J Korean Acad Conserv Dent 2006;31(4):300-311.   Published online July 31, 2006
DOI: https://doi.org/10.5395/JKACD.2006.31.4.300
AbstractAbstract PDFPubReaderePub

The purpose of this study is to evaluate prospectively the effect of different bonding systems and retention grooves on the clinical performance of resin restorations in non-carious cervical lesions (NCCLs). Thirty-nine healthy adults who had at least 2 NCCLs in their premolar areas were included in this study. One hundred and fifty teeth were equally assigned to six groups: (A) Scotchbond Multi-Purpose (SBMP, 3M ESPE, St. Paul, MN, USA, 4th generation bonding system) without retention grooves; (B) SBMP with retention grooves; (C) BC Plus (Vericom Co., Anyang, Gyeonggido, Korea, 5th generation bonding system) without retention grooves; (D) BC Plus with retention grooves; (E) Adper Prompt (3M ESPE, Seefeld, Germany, 6th generation bonding system) without retention grooves; (F) Adper Prompt with retention grooves. All cavities were filled with a hybrid composite resin, Denfil (Vericom Co., Anyang, Gyeonggido, Korea) by one operator. Restorations were evaluated at baseline and at 6-month recall, according to the modified USPHS (United States Public Health Service) criteria. Additionally, clinical photographs were taken and epoxy resin replicas were made for SEM evaluation. At 6-month recall, there were some differences in the number of alpha ratings among the experimental groups. But, despite the differences in the number of alpha ratings, there was no significant difference among the 3 adhesive systems (p > 0.05). There was also no significant difference between the groups with or without mechanical retention (p > 0.05). Follow-ups for longer periods than 6 months are needed to verify the clinical performance of different bonding systems and retention grooves.

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A clinical evaluation of a bleaching strip containing 2.9% hydrogen peroxide
Eun-Sook Park, So-Rae Seong, Seong-Tae Hong, Ji-Eun Kim, So-Young Lee, Soo-Youn Hwang, Shin-Jae Lee, Bo-Hyoung Jin, Ho-Hyun Son, Byeong-Hoon Cho
J Korean Acad Conserv Dent 2006;31(4):269-281.   Published online July 31, 2006
DOI: https://doi.org/10.5395/JKACD.2006.31.4.269
AbstractAbstract PDFPubReaderePub

This study evaluated the effectiveness and safety of an experimental bleaching strip (Medison dental whitening strip, Samsung medical Co., Anyang, Korea) containing 2.9% hydrogen peroxide. Twenty-three volunteers used the bleaching strips for one and a half hour daily for 2 weeks. As control group, the same strips in which hydrogen peroxide was not included were used by 24 volunteers with the same protocol. The shade change (ΔE*, color difference) of twelve anterior teeth was measured using Shade Vision (X-Rite Inc., S.W. Grandville, MI, USA), Chroma Meter (Minolta Co., Ltd. Osaka, Japan) and Vitapan classical shade guide (Vita Zahnfabrik, Germany). The shade change of overall teeth in the experimental group was significantly greater than that in the control group (p < 0.05) and was easily perceivable. The change resulted from the increase of lightness (CIE L* value) and the decrease of redness (CIE a* value) and yellowness (CIE b* value). The shade change of individual tooth was greatest in canine, and smallest in central incisor. The safety of the bleaching strip was also confirmed.

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Distribution of oral pathogens in infections of endodontic origin
Seung-Yoon Kim, Ho-Young Choi, Sang-Hyuk Park, Gi-Woon Choi
J Korean Acad Conserv Dent 2003;28(4):303-313.   Published online July 31, 2003
DOI: https://doi.org/10.5395/JKACD.2003.28.4.303
AbstractAbstract PDFPubReaderePub

It has been documented that periodontopathic bacteria are also implicated in endodontic infections. 16S rDNA gene-directed PCR was to examine the prevalence of periodontopathic bacteria including Actinobacillus actinomycetemcomitans (Aa), Prevotella intermedia (Pi), Prevotella nigrescens (Pn), Porphyromonas gingivalis (Pg), Porphyromonas endodontalis (Pe), and Treponema denticola (Td) in the root canals of 36 endodontically infected teeth having apical lesions with or without clinical symptoms like pain, swelling, and fistula.

In 36 infected root canals, most frequently detected bacterial species was Pg (61.1%), followed by Td (52.8%) and Pe (38.9%).

Of 36 infected root canals, Aa was detected in 6 canals (16.7%) of the teeth, all of which showed clinical symptoms.

Of 36 infected root canals, Pi and Pn were found in 4 (13.9%) and 5 (33.3%), respectively. Notably, prevalence of Pn in the symptomatic teeth was 50.0%.

One of black-pigmented anaerobic bacteria (BPB) including Pi, Pn, Pe, and Pg was detected in all of the teeth that showed pain or especially swelling but not fistula. It was, however, found that prevalence of BPB in the asymptomatic teeth or the teeth with fistula was only 40%.

Pe and Pg were detected in the teeth regardless of the presence or absence of symptoms.

Td was detected in the teeth regardless of the presence or absence of symptoms.

High prevalence of BPB in the symptomatic teeth but low in the asymptomatic teeth suggests that BPB may play an important role in the pathogenesis of periapical lesions.

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