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Research Articles
Prevalence of salivary microbial load and lactic acid presence in diabetic and non-diabetic individuals with different dental caries stages
Monika Mohanty, Shashirekha Govind, Shakti Rath
Restor Dent Endod 2024;49(1):e4.   Published online January 12, 2024
DOI: https://doi.org/10.5395/rde.2024.49.e4
AbstractAbstract PDFPubReaderePub
Objectives

This study aims to correlate caries-causing microorganism load, lactic acid estimation, and blood groups to high caries risk in diabetic and non-diabetic individuals and low caries risk in healthy individuals.

Materials and Methods

This study includes 30 participants divided into 3 groups: Group A, High-risk caries diabetic individuals; Group B, High-risk caries non-diabetic individuals; and Group C, Low-risk caries individuals. The medical condition, oral hygiene, and caries risk assessment (American Dental Association classification and International Caries Detection and Assessment System scoring) were documented. Each individual’s 3 mL of saliva was analyzed for microbial load and lactic acid as follows: Part I: 2 mL for microbial quantity estimation using nutrient agar and blood agar medium, biochemical investigation, and carbohydrate fermentation tests; Part II: 0.5 mL for lactic acid estimation using spectrophotometric analysis. Among the selected individuals, blood group correlation was assessed. The χ2 test, Kruskal-Wallis test, and post hoc analysis were done using Dunn’s test (p < 0.05).

Results

Group A had the highest microbial load and lactic acid concentration, followed by Groups B and C. The predominant bacteria were Lactobacilli (63.00 ± 15.49) and Streptococcus mutans (76.00 ± 13.90) in saliva. Blood Group B is prevalent in diabetic and non-diabetic high-risk caries patients but statistically insignificant.

Conclusions

Diabetic individuals are more susceptible to dental caries due to high microbial loads and increased lactic acid production. These factors also lower the executing tendency of neutrophils, which accelerates microbial accumulation and increases the risk of caries in diabetic individuals.

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Can silver diamine fluoride or silver nanoparticle-based anticaries agents to affect enamel bond strength?
Jaqueline Costa Favaro, Yana Cosendey Toledo de Mello Peixoto, Omar Geha, Flaviana Alves Dias, Ricardo Danil Guiraldo, Murilo Baena Lopes, Sandrine Bittencourt Berger
Restor Dent Endod 2021;46(1):e7.   Published online January 12, 2021
DOI: https://doi.org/10.5395/rde.2021.46.e7
AbstractAbstract PDFPubReaderePub
Objectives

The aim of the current study is to investigate the effect of different anticaries agents, such as experimental agents based on silver nanoparticles (SNPs) and silver diamine fluoride (SDF), on the micro-shear bond strength (μ-SBS) of composite resin applied to intact enamel (IE) or demineralized enamel (DE).

Materials and Methods

Sixty dental enamel fragments were collected from human third molars and categorized into 6 groups (n = 10): positive control (IE), negative control (DE), IE + SDF, DE + SDF, IE + SNP and DE + SNP. Samples from DE, DE + SDF and DE + SNP groups were subjected to pH cycling; superficial microhardness test was performed to confirm demineralization. Resin composite build-ups were applied to the samples (0.75-mm diameter and 1-mm height) after the treatments (except for IE and DE groups); μ-SBS was also evaluated. Samples were analyzed under a stereomicroscope at 40× magnification to identify failure patterns. Data were subjected to one-way analysis of variance, followed by Tukey's and Dunnett's tests (p < 0.05).

Results

There was no significant difference among the IE, IE + SNP, DE + SDF, and DE + SNP groups. The IE + SDF and DE groups recorded the highest and the lowest μ-SBS values, respectively. Adhesive-type failures were the most frequent for all treatments.

Conclusions

Anticaries agents did not have a negative effect on the μ-SBS of composite resin when it was used on IE or DE.

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Case Reports
Functional and aesthetic rehabilitation in posterior tooth with bulk-fill resin composite and occlusal matrix
Luciana Fávaro Francisconi-dos-Rios, Johnny Alexandre Oliveira Tavares, Luanderson Oliveira, Jefferson Chaves Moreira, Flavia Pardo Salata Nahsan
Restor Dent Endod 2020;45(1):e9.   Published online January 3, 2020
DOI: https://doi.org/10.5395/rde.2020.45.e9
AbstractAbstract PDFPubReaderePub

The restorative procedure in posterior teeth involves clinical steps related to professional skill, especially when using the incremental technique, which may fail in the long term. A recent alternative is bulk-fill resins, which can reduce polymerization shrinkage, decreasing clinical problems such as marginal leakage, secondary caries, and fracture. This scientific study aims to report a clinical case using bulk-fill resin with an occlusal matrix. As determined in the treatment plan, an acrylic resin matrix was produced to establish an improved oral and aesthetic rehabilitation of the right mandibular first molar, which presented a carious lesion with dentin involvement. The occlusal matrix is a simple technique that maintains the original dental anatomy, showing satisfactory results regarding function and aesthetic rehabilitation.

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Management of large class II lesions in molars: how to restore and when to perform surgical crown lengthening?
Ana Belén Dablanca-Blanco, Juan Blanco-Carrión, Benjamín Martín-Biedma, Purificación Varela-Patiño, Alba Bello-Castro, Pablo Castelo-Baz
Restor Dent Endod 2017;42(3):240-252.   Published online August 3, 2017
DOI: https://doi.org/10.5395/rde.2017.42.3.240
AbstractAbstract PDFPubReaderePub

The restoration of endodontic tooth is always a challenge for the clinician, not only due to excessive loss of tooth structure but also invasion of the biological width due to large decayed lesions. In this paper, the 7 most common clinical scenarios in molars with class II lesions ever deeper were examined. This includes both the type of restoration (direct or indirect) and the management of the cavity margin, such as the need for deep margin elevation (DME) or crown lengthening. It is necessary to have the DME when the healthy tooth remnant is in the sulcus or at the epithelium level. For caries that reaches the connective tissue or the bone crest, crown lengthening is required. Endocrowns are a good treatment option in the endodontically treated tooth when the loss of structure is advanced.

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Research Articles
Comparison of two different methods of detecting residual caries
Uzay Koç Vural, Zeynep Bilge Kütük, Esra Ergin, Filiz Yalçın Çakır, Sevil Gürgan
Restor Dent Endod 2017;42(1):48-53.   Published online January 25, 2017
DOI: https://doi.org/10.5395/rde.2017.42.1.48
AbstractAbstract PDFPubReaderePub
Objectives

The aim of this study was to investigate the ability of the fluorescence-aided caries excavation (FACE) device to detect residual caries by comparing conventional methods in vivo.

Materials and Methods

A total of 301 females and 202 males with carious teeth participated in this study. The cavity preparations were done by grade 4 (Group 1, 154 teeth), grade 5 (Group 2, 176 teeth), and postgraduate (Group 3, 173 teeth) students. After caries excavation using a handpiece and hand instruments, the presence of residual caries was evaluated by 2 investigators who were previously calibrated for visual-tactile assessment with and without magnifying glasses and trained in the use of a FACE device. The tooth number, cavity type, and presence or absence of residual caries were recorded. The data were analyzed using the Chi-square test, the Fisher's Exact test, or the McNemar test as appropriate. Kappa statistics was used for calibration. In all tests, the level of significance was set at p = 0.05.

Results

Almost half of the cavities prepared were Class II (Class I, 20.9%; Class II, 48.9%; Class III, 20.1%; Class IV, 3.4%; Class V, 6.8%). Higher numbers of cavities left with caries were observed in Groups 1 and 2 than in Group 3 for all examination methods. Significant differences were found between visual inspection with or without magnifying glasses and inspection with a FACE device for all groups (p < 0.001). More residual caries were detected through inspection with a FACE device (46.5%) than through either visual inspection (31.8%) or inspection with a magnifying glass (37.6%).

Conclusions

Within the limitations of this study, the FACE device may be an effective method for the detection of residual caries.

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Elemental analysis of caries-affected root dentin and artificially demineralized dentin
Young-Hye Sung, Ho-Hyun Son, Keewook Yi, Juhea Chang
Restor Dent Endod 2016;41(4):255-261.   Published online August 19, 2016
DOI: https://doi.org/10.5395/rde.2016.41.4.255
AbstractAbstract PDFPubReaderePub
Objectives

This study aimed to analyze the mineral composition of naturally- and artificially-produced caries-affected root dentin and to determine the elemental incorporation of resin-modified glass ionomer (RMGI) into the demineralized dentin.

Materials and Methods

Box-formed cavities were prepared on buccal and lingual root surfaces of sound human premolars (n = 15). One cavity was exposed to a microbial caries model using a strain of Streptococcus mutans. The other cavity was subjected to a chemical model under pH cycling. Premolars and molars with root surface caries were used as a natural caries model (n = 15). Outer caries lesion was removed using a carbide bur and a hand excavator under a dyeing technique and restored with RMGI (FujiII LC, GC Corp.). The weight percentages of calcium (Ca), phosphate (P), and strontium (Sr) and the widths of demineralized dentin were determined by electron probe microanalysis and compared among the groups using ANOVA and Tukey test (p < 0.05).

Results

There was a pattern of demineralization in all models, as visualized with scanning electron microscopy. Artificial models induced greater losses of Ca and P and larger widths of demineralized dentin than did a natural caries model (p < 0.05). Sr was diffused into the demineralized dentin layer from RMGI.

Conclusions

Both microbial and chemical caries models produced similar patterns of mineral composition on the caries-affected dentin. However, the artificial lesions had a relatively larger extent of demineralization than did the natural lesions. RMGI was incorporated into the superficial layer of the caries-affected dentin.

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The effect of different fluoride application methods on the remineralization of initial carious lesions
Seon Mi Byeon, Min Ho Lee, Tae Sung Bae
Restor Dent Endod 2016;41(2):121-129.   Published online May 10, 2016
DOI: https://doi.org/10.5395/rde.2016.41.2.121
AbstractAbstract PDFPubReaderePub
Objectives

The purpose of this study was to assess the effect of single and combined applications of fluoride on the amount of fluoride release, and the remineralization and physical properties of enamel.

Materials and Methods

Each of four fluoride varnish and gel products (Fluor Protector, FP, Ivoclar Vivadent; Tooth Mousse Plus, TM, GC; 60 Second Gel, A, Germiphene; CavityShield, CS, 3M ESPE) and two fluoride solutions (2% sodium fluoride, N; 8% tin(ii) fluoride, S) were applied on bovine teeth using single and combined methods (10 per group), and then the amount of fluoride release was measured for 4 wk. The electron probe microanalysis and the Vickers microhardness measurements were conducted to assess the effect of fluoride application on the surface properties of bovine teeth.

Results

The amount of fluoride release was higher in combined applications than in single application (p < 0.05). Microhardness values were higher after combined applications of N with FP, TM, and CS than single application of them, and these values were also higher after combined applications of S than single application of A (p < 0.05). Ca and P values were higher in combined applications of N with TM and CS than single application of them (p < 0.05). They were also increased after combined applications of the S with A than after single application (p < 0.05).

Conclusions

Combined applications of fluoride could be used as a basis to design more effective methods of fluoride application to provide enhanced remineralization.

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How to design in situ studies: an evaluation of experimental protocols
Young-Hye Sung, Hae-Young Kim, Ho-Hyun Son, Juhea Chang
Restor Dent Endod 2014;39(3):164-171.   Published online May 13, 2014
DOI: https://doi.org/10.5395/rde.2014.39.3.164
AbstractAbstract PDFPubReaderePub
Objectives

Designing in situ models for caries research is a demanding procedure, as both clinical and laboratory parameters need to be incorporated in a single study. This study aimed to construct an informative guideline for planning in situ models relevant to preexisting caries studies.

Materials and Methods

An electronic literature search of the PubMed database was performed. A total 191 of full articles written in English were included and data were extracted from materials and methods. Multiple variables were analyzed in relation to the publication types, participant characteristics, specimen and appliance factors, and other conditions. Frequencies and percentages were displayed to summarize the data and the Pearson's chi-square test was used to assess a statistical significance (p < 0.05).

Results

There were many parameters commonly included in the majority of in situ models such as inclusion criteria, sample sizes, sample allocation methods, tooth types, intraoral appliance types, sterilization methods, study periods, outcome measures, experimental interventions, etc. Interrelationships existed between the main research topics and some parameters (outcome measures and sample allocation methods) among the evaluated articles.

Conclusions

It will be possible to establish standardized in situ protocols according to the research topics. Furthermore, data collaboration from comparable studies would be enhanced by homogeneous study designs.

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Review Article
Early caries detection using optical coherence tomography: a review of the literature
Young-Seok Park, Byeong-Hoon Cho, Seung-Pyo Lee, Won-Jun Shon
J Korean Acad Conserv Dent 2011;36(5):367-376.   Published online September 14, 2011
DOI: https://doi.org/10.5395/JKACD.2011.36.5.367
AbstractAbstract PDFPubReaderePub
Abstract

Early detection of carious lesions increases the possibility of treatment without the need for surgical intervention. Optical coherence tomography (OCT) is an emerging three-dimensional imaging technique that has been successfully used in other medical fields, such as ophthalmology for optical biopsy, and is a prospective candidate for early caries detection. The technique is based on low coherence interferometry and is advantageous in that it is non-invasive, does not use ionizing radiation, and can render three-dimensional images. A brief history of the development of this technique and its principles are discussed in this paper. There have been numerous studies on caries detection, which were mostly in vitro or ex vivo experiments. Through these studies, the feasibility of OCT for caries detection was confirmed. However, further research should be performed, including in vivo studies of OCT applications, in order to prove the clinical usefulness of this technique. In addition, some technological problems must be resolved in the near future to allow for the use of OCT in everyday practice.

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Basic Research
Elemental analysis of the fluoride varnish effects on root caries initiation
Se-Eun Park, Keewook Yi, Hae-Young Kim, Ho-Hyun Son, Juhea Chang
J Korean Acad Conserv Dent 2011;36(4):290-299.   Published online July 31, 2011
DOI: https://doi.org/10.5395/JKACD.2011.36.4.290
AbstractAbstract PDFPubReaderePub
Objectives

The usage of fluoride varnish for a moderate to low caries-risk group has not been well validated. This study aimed to evaluate the preventive and therapeutic efficacies of fluoride varnish on the initiated root caries.

Materials and Methods

Ten premolars were sectioned into quarters, further divided into two windows, one of which was painted with Fluor Protector (1,000 ppm fluoride, Ivoclar Vivadent). An initial lesion with a well-preserved surface layer was produced by pH cycling. Scanned line analysis using energy dispersive spectrometry determined the weight percentages of Ca and P in the demineralized layer. Scanning Electron microscopy and confocal laser scanning microscopy (CLSM) evaluated the varnish-applied root surfaces.

Results

The mean lesion depth (SD) was 12.3 (2.6) µm (single cycling) and 19.6 (3.8) µm (double cycling). Double cycling extended the lesion depth, but induced no more mineral loss than single cycling (p < 0.05). The mean weight percentages of Ca and P between groups with and without varnish were not significantly different (p < 0.05). A CLSM showed varnish remained within 15 µm of the surface layer.

Conclusions

When a mild acid challenge initiated root tissue demineralization, the application of low-concentration fluoride varnish did not influence the lesion depth or the mineral composition of the subsurface lesion.

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Review Article
Theory of X-ray microcomputed tomography in dental research: application for the caries research
Young-Seok Park, Kwang-Hak Bae, Juhea Chang, Won-Jun Shon
J Korean Acad Conserv Dent 2011;36(2):98-107.   Published online March 31, 2011
DOI: https://doi.org/10.5395/JKACD.2011.36.2.98
AbstractAbstract PDFPubReaderePub

Caries remains prevalent throughout modern society and is the main disease in the field of dentistry. Although studies of this disease have used diverse methodology, recently, X-ray microtomography has gained popularity as a non-destructive, 3-dimensional (3D) analytical technique, and has several advantages over the conventional methods. According to X-ray source, it is classified as monochromatic or polychromatic with the latter being more widely used due to the high cost of the monochromatic source despite some advantages. The determination of mineral density profiles based on changes in X-ray attenuation is the principle of this method and calibration and image processing procedures are needed for the better image and reproducible measurements. Using this tool, 3D reconstruction is also possible and it enables to visualize the internal structures of dental caries. With the advances in the computer technology, more diverse applications are being studied, such automated caries assessment algorithms.

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Original Articles
The effect of lactic acid concentration and ph of lactic acid buffer solutions on enamel remineralization
Jung-Won Kwon, Duk-Gyu Suh, Yun-Jung Song, Yun Lee, Chan-Young Lee
J Korean Acad Conserv Dent 2008;33(6):507-517.   Published online November 30, 2008
DOI: https://doi.org/10.5395/JKACD.2008.33.6.507
AbstractAbstract PDFPubReaderePub

There are considerable in vitro and in vivo evidences for remineralization and demineralization occurring simultaneously in incipient enamel caries. In order to "heal"the incipient dental caries, many experiments have been carried out to determine the optimal conditions for remineralization. It was shown that remineralization is affected by different pH, lactic acid concentrations, chemical composition of the enamel, fluoride concentrations, etc.

Eighty specimens from sound permanent teeth without demineralization or cracks, 0.15 mm in thickness, were immersed in lactic acid buffered demineralization solutions for 3 days. Dental caries with a surface zone and subsurface lesion were artificially produced. Groups of 10 specimens were immersed for 10 or 12 days in lactic acid buffered remineralization solutions consisting of pH 4.3 or pH 6.0, and 100, 50, 25, or 10 mM lactic acid. After demineralization and remineralization, images were taken by polarizing microscopy (x100) and micro-computed tomography. The results were obtained by observing images of the specimens and the density of the caries lesions was determined.

As the lactic acid concentration of the remineralization solutions with pH 4.3 was higher, the surface zone of the carious enamel increased and an isotropic zone of the subsurface lesion was found. However, the total decalcification depth increased at the same time.

In the remineralization solutions with pH 6.0, only the surface zone increased slightly but there was no significant change in the total decalcification depth and subsurface zone.

In the lactic acid buffer solutions with the lower pH and higher lactic acid concentration, there were dynamic changes at the deep area of the dental carious lesion.

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The influence of pH and lactic acid concentration on the formation of artificial root caries in acid buffer solution
Hyun-Suk Oh, Byoung-Duck Roh, Chan-Young Lee
J Korean Acad Conserv Dent 2007;32(1):47-60.   Published online January 31, 2007
DOI: https://doi.org/10.5395/JKACD.2007.32.1.047
AbstractAbstract PDFPubReaderePub

The purpose of this study is to compare and to evaluate the effect of pH and lactic acid concentration on the progression of artificial root caries lesion using polarizing microscope, and to evaluate the morphological changes of hydroxyapatite crystals of the demineralized area and to investigate the process of demineralization using scanning electron microscope.

Artificial root caries lesion was created by dividing specimens into 3 pH groups (pH 4.3, 5.0, 5.5), and each pH group was divided into 3 lactic acid concentration groups (25 mM, 50 mM, 100 mM). Each group was immersed in acid buffer solution for 5 days and examined. The results were as follows:

1. Under polarized microscope, the depth of lesion was more effected by the lactic acid concentration rather than the pH.

2. Under scanning electron microscope, dissolution of hydroxyapatite crystals were increased as the lactic acid concentration increased and the pH decreased.

3. Demineralized hydroxyapatite crystals showed peripheral dissolution and decreased size and number within cluster of hydroxyapatite crystals and widening of intercluster and intercrystal spaces as the pH decreased and the lactic acid concentration increased.

4. Under scanning electron microscope evaluation of the surface zone, clusters of hydroxyapatite crystals were dissolved, and dissolution and reattachment of crystals on the surface of collagen fibrils were observed as the lactic acid concentration increased.

5. Under scanning electron microscope, demineralization of dentin occurred not only independently but also with remineralization simultaneously.

In conclusion, the study showed that pH and lactic acid concentration influenced the rate of progression of the lesion in artificial root caries. Demineralization process was progressed from the surface of the cluster of hydroxyapatite crystals and the morphology of hydroxyapatite crystals changed from round or elliptical shape into irregular shape as time elapsed.

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The efficacy of chemo-mechanical removal of dentin carious lesion
Soon-Bin Lim, Kyung-Kyu Choi, Sang-Jin Park
J Korean Acad Conserv Dent 2005;30(3):149-157.   Published online May 31, 2005
DOI: https://doi.org/10.5395/JKACD.2005.30.3.149
AbstractAbstract PDFPubReaderePub

Mechanical removals in decayed teeth have been performed using drill and sharp hand instruments. These methods have some disadvantages such as pain, local anesthesia and overextended cavities. Therefore chemo-mechanical excavation of dentin carious lesions has been introduced. The purpose of this study was to evaluate the efficacy of traditional mechanical methods using burs and chemo-mechanical methods (Carisolv) of caries dentin.

Mechanical caries removal was carried with low speed round bur. Chemo-mechanical caries excavation was performed with Carisolv (Medi-team), using the Carisolv hand instruments. The mean time to remove caries with two different methods was evaluated and the data analyzed with SPSS software (ver 11.5) by t-test (p < 0.05). For histomorphometry of caries removal were also carried with mechanical or chemo-mechanical (Carisolv) methods from 20 extracted caries permanent molars. Complete caries removal was verified with a #23 sharp explorers, Caries Detector (Kuraray Co. Japan), and standard apical radiography.

1. Chemo-mechanical method was taken more times than mechanical method (1.5 fold) (p < 0.05).

2. Excavation for caries took more time for molar lesion than premolar lesion, and the least time was taken to remove the caries in incisor lesion (p < 0.05).

3. There were no significant differences to remove the caries between the maxilla and mandible (p > 0.05).

4. The remaining carious dentin was detected after the chemo-mechanical removal of the carious dentin, and no smear layer were seen after the mechanical and chemo-mechanical removal of the carious dentin.

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Microtensile bond strength of all-in-one adhesive to caries-affected dentin
Ji-Deok Moon, Jeong-Kil Park, Bock Hur, Hyeon-Cheol Kim
J Korean Acad Conserv Dent 2005;30(1):49-57.   Published online January 31, 2005
DOI: https://doi.org/10.5395/JKACD.2005.30.1.049
AbstractAbstract PDFPubReaderePub

The purpose of this study was to evaluate the effect of multiple application of all-in-one dentin adhesive system on microtensile bond strength to caries-affected dentin.

Twenty one extracted human molars with occlusal caries extending into mid-dentin were prepared by grinding the occlusal surface flat. The carious lesions were excavated with the aid of caries detector dye. The following adhesives were applied to caries-affected dentin according to manufacturer's directions; Scotchbond™ Multi-Purpose in SM group, Adper Prompt L-Pop™ 1 coat in LP1 group, 2 coats in LP2 group, 3 coats in LP3 group, Xeno® III 1 coat in XN1 group, 2 coats in XN2 group, and 3 coats in XN3 group. After application of the adhesives, a cylinder of resin-based composite was built up on the occlusal surface. Each tooth was sectioned vertically to obtain the 1 × 1 mm2 sticks. The microtensile bond strength was determined. Each specimen was observed under SEM to examine the failure mode. Data were analyzed with one-way ANOVA.

The results of this study were as follows;

1. The microtensile bond strength values were; SM (14.38 ± 2.01 MPa), LP1 (9.15 ± 1.81 MPa), LP2 (14.08 ± 1.75 MPa), LP3 (14.06 ± 1.45 MPa), XN1 (13.65 ± 1.95 MPa), XN2 (13.98 ± 1.60 MPa), XN3 (13.88 ± 1.66 MPa). LP1 was significantly lower than the other groups in bond strength (p < 0.05). All groups except LP1 were not significantly different in bond strength (p > 0.05).

2. In LP1, there were a higher number of specimens showing adhesive failure. Most specimens of all groups except LP1 showed mixed failure.

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In vivo quantitative analysis of remineralization effect of remineralization solution "R" of incipient enamel dental caries
Myung-Eun Kim, Il-young Jung, Kee-Yeon Kum, Chang-young Lee, Byoung-Duck Roh
J Korean Acad Conserv Dent 2002;27(2):175-182.   Published online March 31, 2002
DOI: https://doi.org/10.5395/JKACD.2002.27.2.175
AbstractAbstract PDFPubReaderePub

Dental caries is a chronic disease that causes the destruction of tooth structure by the interaction of plaque bacteria, food debris, and saliva.

There has been attempts to induce remineralization by supersaturating the intra-oral environment around the surface enamel, where there is incipient caries.

In this study, supersaturated remineralized solution "R" was applied to specimens with incipient enamel caries, and the quantitative ananlysis of remineralization was evaluated using microradiography. Thirty subjects volunteered to participate in this study. Removable appliances were constructed for the subjects, and the enamel specimen with incipient caries were embedded in the appliances. The subjects wore the intra-oral appliance for 15 days except while eating and sleeping.

The removable appliance were soaked in supersaturated solution "R", saline, or Senstime® to expose the specimen to those solutions three times a day, 5 minutes each time. After 15 days, microradiography was retaken to compare and evaluate remineralization.

The results were as the following:

1. The ratio of remineralized area to demineralized area was significantly higher in the supersaturated solution "R" and Senstime® than in the saline. (p<0.05)

2. Remineralization in the supersaturated buffer solution "R" occurred in the significantly deeper parts of the tooth, compared to the Senstime® group containing high concentration of fluoride.(p<0.05)

As in the above results, the remineralization effect of remineralized buffer solution "R" on incipient enamel caries has been proven. For clinical utilization, further studies on soft tissue reaction and the effect on dentin and cementum are necessary.

In conclusion compared to commercially available fluoride solution, remineralization solution "R" showed better remineralization effect on early enamel caries lesion, so it is considered as effecient solution for clinical application.

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