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Research Articles
Pomegranate extract on eroded dentin: antioxidant action, bond strength and morphology of the adhesive interface after aging
Thiago Vinícius Cortez, Nathália Mancioppi Cerqueira, Julia Adornes Gallas, Wanderley Pereira Oliveira, Silmara Aparecida Milori Corona, Aline Evangelista Souza-Gabriel
Restor Dent Endod 2024;49(1):e9.   Published online January 26, 2024
DOI: https://doi.org/10.5395/rde.2024.49.e9
AbstractAbstract PDFPubReaderePub
Objectives

This study aimed to evaluate the effect of pomegranate solution (Punica granatum) on eroded dentin through antioxidant action, shear bond strength (SBS) and interface morphology.

Materials and Methods

The 10% pomegranate peel extract was prepared by the lyophilization method. Punicalagin polyphenol was confirmed by high-performance liquid chromatography. Antioxidant activity was evaluated by capturing the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. For the SBS, 48 dentin fragments were divided into sound or eroded, and subdivided according to the pretreatment (n = 12): water or P. granatum. The surfaces were restored with self-etch adhesive and a bulk-fill resin (Ecosite; DMG). The SBS was done immediately (24 hours) and after thermal cycling + water storage (12 months). For scanning electron microscopy, 48 dentin fragments (24 sound and 24 eroded) received the same treatments as for SBS (n = 6), and they were analyzed after 24 hours and 12 months.

Results

The P. granatum had antioxidant action similar (p = 0.246) to the phenolic standard antioxidants. After 24 hours, eroded dentin had lower SBS than sound dentin (p < 0.001), regardless of the pretreatment. After 12 months, P. granatum maintained the SBS of sound dentin (13.46 ± 3.42 MPa) and eroded dentin (10.96 ± 1.90 MPa) statistically similar. The lowest values were found on eroded dentin treated with water (5.75 ± 1.65 MPa) (p < 0.001). P. granatum on eroded dentin caused peritubular demineralization and hybrid layer with resin tags.

Conclusions

The pomegranate extract had antioxidant action and preserved the adhesive interface of the eroded dentin.

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Is dentin biomodification with collagen cross-linking agents effective for improving dentin adhesion? A systematic review and meta-analysis
Julianne Coelho Silva, Edson Luiz Cetira Filho, Paulo Goberlânio de Barros Silva, Fábio Wildson Gurgel Costa, Vicente de Paulo Aragão Saboia
Restor Dent Endod 2022;47(2):e23.   Published online May 6, 2022
DOI: https://doi.org/10.5395/rde.2022.47.e23
AbstractAbstract PDFSupplementary MaterialPubReaderePub
Objectives

The aim of this investigation was to evaluate the effectiveness of collagen cross-linking agents (CCLAs) used in combination with the adhesive technique in restorative procedures.

Materials and Methods

In this systematic review, the authors followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses checklist. An electronic search was performed using PubMed, Scopus, Web of Science, Cochrane Library, LILACS, and DOSS, up to October 2020. The gray literature was also researched. Only randomized clinical trials were selected.

Results

The selection process yielded 3 studies from the 838 retrieved. The addition of CCLAs in the retention of restorations increased the number of events. The postoperative sensitivity scores and marginal adaptation scores showed no significant difference between the CCLA and control groups, and the marginal pigmentation scores showed a significant increase in the CCLA group. There were no caries events in any group throughout the evaluation period.

Conclusions

This systematic review showed that there is no clinical efficacy to justify the use of CCLAs in the protocols performed.

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Do universal adhesives promote bonding to dentin? A systematic review and meta-analysis
Ali A. Elkaffas, Hamdi H. H. Hamama, Salah H. Mahmoud
Restor Dent Endod 2018;43(3):e29.   Published online June 18, 2018
DOI: https://doi.org/10.5395/rde.2018.43.e29
AbstractAbstract PDFPubReaderePub
Objectives

The aims of this study were to conduct a systematic review of the microtensile bond strength (µTBS) of multi-mode adhesives to dentin and to perform a meta-analysis to assess the significance of differences in the µTBS of one of the most commonly used universal adhesives (Scotchbond Universal, 3M ESPE) depending on whether the etch-and-rinse or self-etch mode was used.

Materials and Methods

An electronic search was performed of MEDLINE/PubMed, ScienceDirect, and EBSCOhost. Laboratory studies that evaluated the µTBS of multi-mode adhesives to dentin using either the etch-and-rinse or self-etch mode were selected. A meta-analysis was conducted of the reviewed studies to quantify the differences in the µTBS of Scotchbond Universal adhesive.

Results

Only 10 studies fulfilled the inclusion criteria for the systematic review. Extensive variation was found in the restorative materials, testing methodologies, and failure mode in the reviewed articles. Furthermore, variation was also observed in the dimensions of the microtensile testing beams. The meta-analysis showed no statistically significant difference between the etch-and-rinse and self-etch modes for Scotchbond Universal adhesive (p > 0.05).

Conclusions

Multi-mode ‘universal’ adhesives can achieve substantial bonding to dentin, regardless of the used modes (either etch-and-rinse or self-etch).

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Effects of solvent volatilization time on the bond strength of etch-and-rinse adhesive to dentin using conventional or deproteinization bonding techniques
José Aginaldo de Sousa Júnior, Márcia Luciana Carregosa Santana, Fabricio Eneas Diniz de Figueiredo, André Luis Faria-e-Silva
Restor Dent Endod 2015;40(3):202-208.   Published online March 17, 2015
DOI: https://doi.org/10.5395/rde.2015.40.3.202
AbstractAbstract PDFPubReaderePub
Objectives

This study determined the effect of the air-stream application time and the bonding technique on the dentin bond strength of adhesives with different solvents. Furthermore, the content and volatilization rate of the solvents contained in the adhesives were also evaluated.

Materials and Methods

Three adhesive systems with different solvents (Stae, SDI, acetone; XP Bond, Dentsply De Trey, butanol; Ambar, FGM, ethanol) were evaluated. The concentrations and evaporation rates of each adhesive were measured using an analytical balance. After acid-etching and rinsing, medium occlusal dentin surfaces of human molars were kept moist (conventional) or were treated with 10% sodium hypochlorite for deproteinization. After applying adhesives over the dentin, slight air-stream was applied for 10, 30 or 60 sec. Composite cylinders were built up and submitted to shear testing. The data were submitted to ANOVA and Tukey's test (α = 0.05).

Results

Stae showed the highest solvent content and Ambar the lowest. Acetone presented the highest evaporation rate, followed by butanol. Shear bond strengths were significantly affected only by the factors of 'adhesive' and 'bonding technique' (p < 0.05), while the factor 'duration of air-stream' was not significant. Deproteinization of dentin increased the bond strength (p < 0.05). Stae showed the lowest bond strength values (p < 0.05), while no significant difference was observed between XP Bond and Ambar.

Conclusions

Despite the differences in content and evaporation rate of the solvents, the duration of air-stream application did not affect the bond strength to dentin irrespective of the bonding technique.

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Original Articles
The effects of dentin bonding agent thickness on stress distribution of composite-tooth interface : Finite element method
Sang-Il Park, Yemi Kim, Byoung-Duk Roh
J Korean Acad Conserv Dent 2009;34(5):442-449.   Published online September 30, 2009
DOI: https://doi.org/10.5395/JKACD.2009.34.5.442
AbstractAbstract PDFPubReaderePub

The aim of this study was to examine that thick dentin bonding agent application or low modulus composite restoration could reduce stresses on dentin bonding agent layer.

A mandibular first premolar with abfraction lesion was modeled by finite element method. The lesion was restored by different composite resins with variable dentin bonding agent thickness (50µm, 100µm, 150µm). 170N of occlusal loading was applied buccally or lingually. Von Mises stress on dentin bonding agent layer were measured.

When thickness of dentin bonding agent was increased von Mises stresses at dentin bonding agent were decreased in both composites. Lower elastic modulus composite restoration showed decreased von Mises stresses. On root dentin margin more stresses were generated than enamel margin.

For occlusal stress relief at dentin boning agent layer to applicate thick dentin bonding agent or to choose low elastic modulus composite is recommended.

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The influence of cavity configuration on the microtensile bond strength between composite resin and dentin
Yemi Kim, Jeong-won Park, Chan young Lee, Yoon jung Song, Deok Kyu Seo, Byoung-Duck Roh
J Korean Acad Conserv Dent 2008;33(5):472-480.   Published online September 30, 2008
DOI: https://doi.org/10.5395/JKACD.2008.33.5.472
AbstractAbstract PDFPubReaderePub

This study was conducted to evaluate the influence of the C-factor on the bond strength of a 6th generation self-etching system by measuring the microtensile bond strength of four types of restorations classified by different C-factors with an identical depth of dentin.

Eighty human molars were divided into four experimental groups, each of which had a C-factor of 0.25, 2, 3 or 4. Each group was then further divided into four subgroups based on the adhesive and composite resin used. The adhesives used for this study were AQ Bond Plus (Sun Medical, Japan) and Xeno III (DENTSPLY, Germany). And composite resins used were Fantasista (Sun Medical, Japan) and Ceram-X mono (DENTSPLY, Germany).

The results were then analyzed using one-way ANOVA, a Tukey's test, and a Pearson's correlation test and were as follows.

There was no significant difference among C-factor groups with the exception of groups of Xeno III and Ceram-X mono (p < 0.05).

There was no significant difference between any of the adhesives and composite resins in groups with C-factor 0.25, 2 and 4.

There was no correlation between the change in C-factor and microtensile bond strength in the Fantasista groups.

It was concluded that the C-factor of cavities does not have a significant effect on the microtensile bond strength of the restorations when cavities of the same depth of dentin are restored using composite resin in conjunction with the 6th generation self-etching system.

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Influence of adhesive application on shear bond strength of the resin cement to indirect resin composite
Mi-Hae Song, Su-Jung Park, Hyun-Gu Cho, Yun-Chan Hwang, Won-Mann Oh, In-Nam Hwang
J Korean Acad Conserv Dent 2008;33(5):419-427.   Published online September 30, 2008
DOI: https://doi.org/10.5395/JKACD.2008.33.5.419
AbstractAbstract PDFPubReaderePub

This study analyzed the influence of dental adhesive/primer on the bond strength between indirect resin composite and the resin cement.

Seventy disc specimens of indirect resin composite (Tescera Dentin, Bisco) were fabricated. And bonding area of all specimens were sandblasted and silane treated for one minute. The resin cements were used with or without application of adhesive/primer to bonding area of indirect resin restoration: Variolink-II (Ivoclar-Vivadent): Exite DSC, Panavia-F (Kuraray): ED-Primer, RelyX Unicem (3M ESPE): Single-Bond, Duolink (Bisco): One-step, Mulitlink (Ivoclar-Vivadent): Multilinh Primer.

Shear bond strength was measured by Instron universal testing machine.

Adhesive application improved shear bond strength (p < 0.05). But Variolink II and Panavia-F showed no statistically significant difference according to the adhesive application.

With the above results, when resin inlay is luted by resin cement it seems that application of dental adhesive/primer is necessary in order to improve the bond strength.

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Shear bond strength of dentin bonding agents cured with a Plasma Arc curing light
Youngchul Kwon, Sun-Young Kim, Sae-Joon Chung, Young-Chul Han, In-Bog Lee, Ho-Hyun Son, Chung-Moon Um, Byeong-Hoon Cho
J Korean Acad Conserv Dent 2008;33(3):213-223.   Published online May 31, 2008
DOI: https://doi.org/10.5395/JKACD.2008.33.3.213
AbstractAbstract PDFPubReaderePub

The objective of this study was to compare dentin shear bond strength (DSBS) of dentin bonding agents (DBAs) cured with a plasma arc (PAC) light curing unit (LCU) and those cured with a light emitting diode (LED) LCU. Optical properties were also analyzed for Elipar freelight 2 (3M ESPE); LED LCU, Apollo 95E (DMT Systems); PAC LCU and VIP Junior (Bisco); Halogen LCU. The DBAs used for DSBS test were Scotchbond Multipurpose (3M ESPE), Singlebond 2 (3M ESPE) and Clearfil SE Bond (Kuraray). After DSBS testing, fractured specimens were analyzed for failure modes with SEM.

The total irradiance and irradiance between 450 nm and 490 nm of the LCUs were different. LED LCU showed narrow spectral distribution around its peak at 462 nm whereas PAC and Halogen LCU showed a broad spectrum. There were no significant differences in mean shear bond strength among different LCUs (P > 0.05) but were significant differences among different DBAs (P < 0.001)

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A study on fractural behavior of dentin-resin interface
Gil-Joo Ryu, Gi-Woon Choi, Sang-Jin Park, Kyung-Kyu Choi
J Korean Acad Conserv Dent 2007;32(3):208-221.   Published online May 31, 2007
DOI: https://doi.org/10.5395/JKACD.2007.32.3.208
AbstractAbstract PDFPubReaderePub

The fracture toughness test is believed as a clinically relevant method for assessing the fracture resistance of the dentinal restoratives. The objectives of this study were to measure the fracture toughness (K1C) and microtensile bond strength of dentin-resin composite interface and compare their relationship for their use in evaluation of the integrity of the dentin-resin bond.

A minimum of six short-rod specimens for fracture toughness test and fifteen specimens for microtensile bond strength test was fabricated for each group of materials used. After all specimens storing for 24 hours in distilled water at 37℃, they were tensile-loaded with an EZ tester universal testing machin. Statistical analysis was performed using ANOVA and Tukey's test at the 95% confidence level, Pearson's coefficient was used to verify the correlation between the mean of fracture toughness and microtensile bond strength. FE-SEM was employed on fractured surface to describe the crack propagation.

Fracture toughness value of Clearfil SE Bond (SE) was the highest, followed by Adper Single Bond 2 (SB), OptiBond Solo (OB), ONE-STEP PLUS (OS), ScotchBond Multi-purpose (SM) and there was significant difference between SE and other 4 groups (p < 0.05). There were, however, no significant difference among SB, OB, OS, SM (p > 0.05). Microtensile bond strength of SE was the highest, followed by SB, OB, SM, OS and OS only showed significant lower value (p < 0.05). There was no correlation between fracture toughness and microtensile bond strength values. FE-SEM examination revealed that dentin bonding agent showed different film thickness and different failure pattern according to the film thickness.

From the limited results of this study, it was noted that there was statistically no correlation between K1C and µTBS. We can conclude that for obtaining the reliability of bond strength test of dentin bonding agent, we must pay more attention to the test procedure and its profound scrutiny.

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The effect of cavity wall property on the shear bond strength test using iris method
Dong-Hwan Kim, Ji-Hyun Bae, Byeong-Hoon Cho, In-Bog Lee, Seung-Ho Baek, Hyun-Mi Ryu, Ho-Hyun Son, Chung-Moon Um, Hyuck-Choon Kwon
J Korean Acad Conserv Dent 2004;29(2):170-176.   Published online March 31, 2004
DOI: https://doi.org/10.5395/JKACD.2004.29.2.170
AbstractAbstract PDFPubReaderePub
Objectives

In the unique metal iris method, the developing interfacial gap at the cavity floor resulting from the cavity wall property during polymerizing composite resin might affect the nominal shear bond strength values. The aim of this study is to evaluate that the iris method reduces the cohesive failure in the substrates and the cavity wall property effects on the shear bond strength tests using iris method.

Materials and Methods

The occlusal dentin of 64 extracted human molars were randomly divided into 4 groups to simulate two different levels of cavity wall property (metal and dentin iris) and two different materials (ONE-STEP® and ALL-BOND® 2) for each wall property. After positioning the iris on the dentin surface, composite resin was packed and light-cured. After 24 hours the shear bond strength was measured at a crosshead speed of 0.5 mm/min. Fracture analysis was performed using a microscope and SEM. The data was analyzed statistically by a two-way ANOVA and t-test.

Results

The shear bond strength with metal iris was significant higher than those with dentin iris (p = 0.034). Using ONE-STEP®, the shear bond strength with metal iris was significant higher than those with dentin iris (p = 0.005), but not in ALL-BOND® 2 (p = 0.774). The incidence of cohesive failure was very lower than other shear bond strength tests that did not use iris method.

Conclusions

The iris method may significantly reduce the cohesive failures in the substrates. According to the bonding agent systems, the shear bond strength was affected by the cavity wall property.

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Comparison of shear bond strength of different bonding systems on bleached enamel
Kwang-Keun Kim, Jeong-Won Park
J Korean Acad Conserv Dent 2004;29(1):30-35.   Published online January 31, 2004
DOI: https://doi.org/10.5395/JKACD.2004.29.1.030
PDFPubReaderePub
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Dentin bond strength of bonding agents cured with Light Emitting Diode
Sun-Young Kim, In-Bog Lee, Byeong-Hoon Cho, Ho-Hyun Son, Mi-Ja Kim, Chang-In Seok, Chung-Moon Um
J Korean Acad Conserv Dent 2004;29(6):504-514.   Published online January 14, 2004
DOI: https://doi.org/10.5395/JKACD.2004.29.6.504
AbstractAbstract PDFPubReaderePub
ABSTRACT

This study compared the dentin shear bond strengths of currently used dentin bonding agents that were irradiated with an LED (Elipar FreeLight, 3M-ESPE) and a halogen light (VIP, BISCO). The optical characteristics of two light curing units were evaluated. Extracted human third molars were prepared to expose the occlusal dentin and the bonding procedures were performed under the irradiation with each light curing unit. The dentin bonding agents used in this study were Scotchbond Multipurpose (3M ESPE), Single Bond (3M ESPE), One-Step (Bisco), Clearfil SE bond (Kuraray), and Adper Prompt (3M ESPE). The shear test was performed by employing the design of a chisel-on-iris supported with a Teflon wall. The fractured dentin surface was observed with SEM to determine the failure mode.

The spectral appearance of the LED light curing unit was different from that of the halogen light curing unit in terms of maximum peak and distribution. The LED LCU (maximum peak in 465 ㎚) shows a narrower spectral distribution than the halogen LCU (maximum peak in 487 ㎚). With the exception of the Clearfil SE bond (P < 0.05), each 4 dentin bonding agents showed no significant difference between the halogen light-cured group and the LED light-cured group in the mean shear bond strength (P > 0.05).

The results can be explained by the strong correlation between the absorption spectrum of cam-phoroquinone and the narrow emission spectrum of LED.

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Effect of dentinal tubules orientation on penetration pattern of dentin adhesives using confocal laser scanning microscopy
Dong-Jun Kim, Yun-Chan Hwang, Sun-Ho Kim, Won-Mann Oh, In-Nam Hwang
J Korean Acad Conserv Dent 2003;28(5):392-401.   Published online September 30, 2003
DOI: https://doi.org/10.5395/JKACD.2003.28.5.392
AbstractAbstract PDFPubReaderePub

The purpose of this study was to evaluate the penetration pattern of dentin adhesives according to the orientation of dentinal tubules with confocal laser scanning microscopy. Specimens having perpendicular, parallel and oblique surface to dentinal tubules were fabricated. The primer of dentin adhesives (ALL BOND® 2, CLEARFIL™ SE BOND and PQ1) was mixed with fluorescent material, rhodamine B isothiocyanate (Aldrich Chem. CO., Milw., USA). It was applied to the specimens according to the instructions of manufactures. The specimens were covered with composite resin (Estelite, shade A2) and then cut to a thickness of 500 µm with low speed saw (Isomet™, Buehler, USA). The adhesive pattern of dentin adhesives were observed by fluorescence image using confocal laser scanning microscopy.

The results were as follows.

For the groups with tubules perpendicular to bonded surface, funnel shape of resin tag was observed in all specimen. However, resin tags were more prominent in phosphoric acid etching system (ALL BOND® 2 and PQ1) than self etching system (CLEARFIL™ SE BOND).

For the groups with tubules parallel to bonded surface, rhodamine-labeled primer penetrated into peritubular dentin parallel to the orientation of dentinal tubules. But rhodamine-labeled primer of PQ1 diffused more radially into surrounding intertubular dentin than other dentin adhesive systems.

For the groups with tubules oblique to bonded surface, resin tags appeared irregular and discontinuous. But they penetrated deeper into dentinal tubules than other groups.

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