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Microbial profile of asymptomatic and symptomatic teeth with primary endodontic infections by pyrosequencing
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Sang-Min Lim, Tae-Kwon Lee, Eun-Jeong Kim, Jun-Hong Park, Yoon Lee, Kwang-Shik Bae, Kee-Yeon Kum
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J Korean Acad Conserv Dent 2011;36(6):498-505. Published online November 30, 2011
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DOI: https://doi.org/10.5395/JKACD.2011.36.6.498
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Abstract
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Objectives
The purpose of this in vivo study was to investigate the microbial diversity in symptomatic and asymptomatic canals with primary endodontic infections by using GS FLX Titanium pyrosequencing.
Materials and Methods
Sequencing was performed on 6 teeth (symptomatic, n = 3; asymptomatic, n = 3) with primary endodontic infections. Amplicons from hypervariable region of the small-subunit ribosomal RNA gene were generated by polymerized chain reaction (PCR), and sequenced by means of the GS FLX Titanium pyrosequencing.
Results
On average, 10,639 and 45,455 16S rRNA sequences for asymptomatic and symptomatic teeth were obtained, respectively. Based on Ribosomal Database Project Classifier analysis, pyrosequencing identified the 141 bacterial genera in 13 phyla. The vast majority of sequences belonged to one of the seven phyla: Actinobacteria, Bacteroidetes, Firmicutes, Fusobacteria, Proteobacteria, Spirochetes, and Synergistetes. In genus level, Pyramidobacter, Streptococcus, and Leptotrichia constituted about 50% of microbial profile in asymptomatic teeth, whereas Neisseria, Propionibacterium, and Tessaracoccus were frequently found in symptomatic teeth (69%). Grouping the sequences in operational taxonomic units (3%) yielded 450 and 1,997 species level phylotypes in asymptomatic and symptomatic teeth, respectively. The total bacteria counts were significantly higher in symptomatic teeth than that of asymptomatic teeth (p < 0.05).
Conclusions
GS FLX Titanium pyrosequencing could reveal a previously unidentified high bacterial diversity in primary endodontic infections.
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Citations
Citations to this article as recorded by 
- Root Canal Microbiome Associated With Asymptomatic Apical Periodontitis as Determined by High-Throughput Sequencing
Rodrigo Rodrigues Amaral, Tiago Braga, José F. Siqueira, Isabela N. Rôças, Caio Tavora Coelho da Costa Rachid, Anna Gabriella Guimarães Oliveira, Maria Ilma de Souza Côrtes, Robert Mattew Love Journal of Endodontics.2022; 48(4): 487. CrossRef - A critical analysis of research methods and experimental models to study the root canal microbiome
José F. Siqueira, Isabela N. Rôças International Endodontic Journal.2022; 55(S1): 46. CrossRef - Oral microbiomes in children with asthma and dental caries
Sergey V. Cherkasov, Larisa Yu. Popova, Tatyana V. Vivtanenko, Rimma R. Demina, Yuri A. Khlopko, Alexander S. Balkin, Andrey O. Plotnikov Oral Diseases.2019; 25(3): 898. CrossRef - Insights into the human oral microbiome
Digvijay Verma, Pankaj Kumar Garg, Ashok Kumar Dubey Archives of Microbiology.2018; 200(4): 525. CrossRef - Solving the etiology of dental caries
Aurea Simón-Soro, Alex Mira Trends in Microbiology.2015; 23(2): 76. CrossRef - Present status and future directions in endodontic microbiology
José F. Siqueira, Isabela N. Rôças Endodontic Topics.2014; 30(1): 3. CrossRef - Application of high-throughput sequencing in understanding human oral microbiome related with health and disease
Hui Chen, Wen Jiang Frontiers in Microbiology.2014;[Epub] CrossRef
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Comparison of gene expression profiles of human dental pulp cells treated with mineral trioxide aggregate and calcium hydroxide
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Yong-Beom Kim, Won-Jun Shon, Woocheol Lee, Kee-Yeon Kum, Seung-Ho Baek, Kwang-Shik Bae
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J Korean Acad Conserv Dent 2011;36(5):397-408. Published online September 14, 2011
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DOI: https://doi.org/10.5395/JKACD.2011.36.5.397
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Abstract
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- Abstract
Objectives:
This study investigated changes in gene expressions concerning of differentiation, proliferation, mineralization and inflammation using Human-8 expression bead arrays when white Mineral Trioxide Aggregate and calcium hydroxide-containing cement were applied in vitro to human dental pulp cells (HDPCs).
Materials and Methods:
wMTA (white ProRoot MTA, Dentsply) and Dycal (Dentsply Caulk) in a Teflon tube (inner diameter 10 mm, height 1 mm) were applied to HDPCs. Empty tube-applied HDPCs were used as negative control. Total RNA was extracted at 3, 6, 9 and 24 hr after wMTA and Dycal application. The results of microarray were confirmed by reverse transcriptase polymerase chain reaction.
Results:
Out of the 24,546 genes, 43 genes (e.g., BMP2, FOSB, THBS1, EDN1, IL11, COL10A1, TUFT1, HMOX1) were up-regulated greater than two-fold and 25 genes (e.g., SMAD6, TIMP2, DCN, SOCS2, CEBPD, KIAA1199) were down-regulated below 50% by wMTA. Two hundred thirty nine genes (e.g., BMP2, BMP6, SMAD6, IL11, FOS, VEGFA, PlGF, HMOX1, SOCS2, CEBPD, KIAA1199) were up-regulated greater than two-fold and 358 genes (e.g., EDN1, FGF) were down-regulated below 50% by Dycal.
Conclusions:
Both wMTA and Dycal induced changes in gene expressions related with differentiation and proliferation of pulp cells. wMTA induced changes in gene expressions related with mineralization, and Dycal induced those related with angiogenesis. The genes related with inflammation were more expressed by Dycal than by wMTA. It was confirmed that both wMTA and Dycal were able to induce gene expression changes concerned with the pulp repair in different ways.
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Citations
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- Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells
Min-Seock Seo, Kyung-Gyun Hwang, Hyongbum Kim, Seung-Ho Baek Restorative Dentistry & Endodontics.2012; 37(3): 142. CrossRef
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Matrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth
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Su-Jung Shin, Woocheol Lee, Jae-Il Lee, Seung-Ho Baek, Kee-Yeon Kum, Won-Jun Shon, Kwang-Shik Bae
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J Korean Acad Conserv Dent 2011;36(3):196-202. Published online May 31, 2011
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DOI: https://doi.org/10.5395/JKACD.2011.36.3.196
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Abstract
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Objectives
The aim of this study was to investigate levels of matrix metalloproteinase-8 (MMP-8) and substance P (SP) in root canal exudates during root canal treatment (RCT) of nonvital, painful teeth.
Materials and Methods
Patients scheduled for nonsurgical RCT were prospectively selected; the study was performed after obtaining informed consent from the patients and was approved by the Institutional Review Board for Clinical Research of Gangnam Severance Hospital, Yonsei University (3-2008-0118). Canal exudates samples were collected using sterilized paper points from teeth scheduled for RCT across three different time periods. MMP-8 and SP levels were measured using enzyme-linked immunosorbent assay (ELISA). Data were analyzed using a mixed model analysis and the Pearson correlation analysis (p < 0.05).
Results
MMP-8 and SP levels in GCF were decreased during RCT (p < 0.0001), and they showed a weak positive correlation to each other (p < 0.05). Patients'subjective pain levels and the response from percussion test were significantly related to SP level.
Conclusions
This study demonstrated that periradicular inflammation endodontic origin can elevate SP and MMP-8 levels in root canal exudates. Interestingly, SP level of canal exudates showed a possibility of being used as an indicator of pain due to periapical pathosis.
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Gene expression profiling in human dental pulp cells treated with mineral trioxide aggregate
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Yong-Beom Kim, Won-Jun Shon, WooCheol Lee, Kee-Yeon Kum, Seung-Ho Baek, Kwang-Shik Bae
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J Korean Acad Conserv Dent 2010;35(3):152-163. Published online May 31, 2010
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DOI: https://doi.org/10.5395/JKACD.2010.35.3.152
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Abstract
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This study investigated the changes in gene expression when mineral trioxide aggregate (MTA) was applied in vitro to human dental pulp cells (HDPCs). MTA in a teflon tube (diameter 10 mm, height 2 mm) was applied to HDPCs. Empty tube-applied HDPCs were used as negative control. For microarray analysis, total RNA was extracted at 6, 24, and 72 hrs after MTA application. The results were confirmed selectively by performing reverse transcriptase polymerase chain reaction for genes that showed changes of more than two-fold or less than half. Of the 24,546 genes, 109 genes were up-regulated greater than two-fold (e.g., FOSB, THBS1, BHLHB2, EDN1, IL11, FN1, COL10A1, and TUFT1) and 69 genes were down-regulated below 50% (e.g., SMAD6 and DCN). These results suggest that MTA, rather than being a bio-inert material, may have potential to affect the proliferation and differentiation of pulp cells in various ways.
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Citations
Citations to this article as recorded by 
- Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells
Min-Seock Seo, Kyung-Gyun Hwang, Hyongbum Kim, Seung-Ho Baek Restorative Dentistry & Endodontics.2012; 37(3): 142. CrossRef - Comparison of gene expression profiles of human dental pulp cells treated with mineral trioxide aggregate and calcium hydroxide
Yong-Beom Kim, Won-Jun Shon, Woocheol Lee, Kee-Yeon Kum, Seung-Ho Baek, Kwang-Shik Bae Journal of Korean Academy of Conservative Dentistry.2011; 36(5): 397. CrossRef
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The relationship of radiographic lesion size and characteristics to diagnosis of periapical cysts and granulomas
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Ho-Sik Choi, Woo-Cheol Lee, Won-Jun Shon, Kee-Yeon Kum, Kwang-Shik Bae, Seung-Ho Baek
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J Korean Acad Conserv Dent 2010;35(1):24-29. Published online January 31, 2010
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DOI: https://doi.org/10.5395/JKACD.2010.35.1.024
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Abstract
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The purpose of this study was to find out the relationship of radiographic lesion size, gender, age of patients and radiographic character to the diagnosis of periapical cyst and granuloma.
The data was collected from 187 periapical lesions of 167 patients who undergone apical surgery at Department of Conservative Dentistry, Seoul National University Dental Hospital from 2003 to 2005. The lesion were surgically removed and send for biopsy to the Oral Pathology Laboratory. From the initial radiograph, lesion size was calculated using PiViewSTAR® (INFINITT, Korea) program. The obtained data were statistically evaluated using SPSS (p < 0.05).
The result were as followings:
From 187 biopsy samples, the incidence of periapical cyst was 28.34% and granuloma was 65.24%.
There was a significant correlation between periapical cyst and the size of radiographic lesion (p < 0.01).
There were no significant correlations between age, gender, location of lesion and the final diagnosis (p > 0.05).
There was a significant correlation between the non-demarcation of the lesion and the incidence of periapical granuloma (p < 0.01).
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The role of Type 2 Diabetes as a predisposing risk factor on the pulpo-periapical pathogenesis: review article
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Jin-Hee Kim, Kwang-shik Bae, Deog-Gyu Seo, Sung-Tae Hong, Yoon Lee, Sam-Pyo Hong, Kee-Yeon Kum
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J Korean Acad Conserv Dent 2009;34(3):169-176. Published online May 31, 2009
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DOI: https://doi.org/10.5395/JKACD.2009.34.3.169
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Abstract
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Diabetes Mellitus (DM) is a syndrome accompanied with the abnormal secretion or function of insulin, a hormone that plays a vital role in controlling the blood glucose level (BGL). Type 1and 2 DM are most common form and the prevalence of the latter is recently increasing. The aim of this article was to assess whether Type 2 DM could act as a predisposing risk factor on the pulpo-periapical pathogenesis. Previous literature on the pathologic changes of blood vessels in DM was thoroughly reviewed. Furthermore, a histopathologic analysis of artificially-induced periapical specimens obtained from Type 2 diabetic and DM-resistant rats was compared. Histopathologic results demonstrate that the size of periapical bone destruction was larger and the degree of pulpal inflammation was more severe in diabetic rats, indicating that Type 2 DM itself can be a predisposing risk factor that makes the host more susceptible to pulpal infection. The possible reasons may be that in diabetic state the lumen of pulpal blood vessels are thickened by atheromatous deposits, and microcirculation is hindered. The function of polymorphonuclear leukocyte is also impaired and the migration of immune cells is blocked, leading to increased chance of pulpal infection. Also, lack of collateral circulation of pulpal blood vessels makes the pulp more susceptible to infection. These decrease the regeneration capacity of pulpal cells or tissues, delaying the healing process. Therefore, when restorative treatment is needed in Type 2 DM patients, dentists should minimize irritation to the pulpal tissue un der control of BGL.
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Citations
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- Pulp necrosis following luxated injury to teeth in a patient with uncontrolled type II diabetes mellitus: a case report
Haneol Shin, Seung-Jong Lee, Il-Young Jung, Chan-Young Lee Restorative Dentistry & Endodontics.2012; 37(1): 61. CrossRef
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IN VITRO EVALUATION OF CLEANING EFFICACY OF VARIOUS IRRIGATION METHODS IN MANDIBULAR MOLARS
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So-Young Lee, Won-Jun Son, WooCheol Lee, Kee-Yeon Kum, Kwang-Shik Bae, Seung-Ho Baek
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J Korean Acad Conserv Dent 2009;34(3):215-222. Published online January 14, 2009
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DOI: https://doi.org/10.5395/JKACD.2009.34.3.215
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- Abstract
The aim of this in vitro study was to evaluate the cleaning efficacy of various irrigation methods in the mandibular mesial roots. The forty five mesial root canals were shaped by Profile .06 instruments to apical size #30 and irrigated with 5 ml of 3.5% NaOCl. The teeth were divided into 3 groups and irrigated finally for 1 minute; Group 1: syringe irrigation, Group 2: ultrasonic irrigation, Group 3: RinsEndo irrigation.
After histological processing, the cross sections of apical 1, 3, and 5 mm level were examined with an optical microscope. The cleanliness values of canals and isthmuses were calculated and analyzed by Mann-Whitney U test.
There were no significant differences in both canal and isthmus cleanliness between syringe irrigation and ultrasonic irrigation except 5 mm level of isthmus.
RinsEndo irrigation had significantly higher canal cleanliness values than syringe irrigation at 1 mm and 3 mm levels (p < 0.05). Also, RinsEndo irrigation had significantly higher isthmus cleanliness values than syringe irrigation at all levels evaluated (p < 0.05).
There were no statistical differences in both canal and isthmus cleanliness between ultrasonic irrigation and RinsEndo irrigation except 3 mm level of canal. From this study, RinsEndo irrigation can be useful as an additional irrigation procedure.
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Citations
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- Multivariate analysis of the cleaning efficacy of different final irrigation techniques in the canal and isthmus of mandibular posterior teeth
Yeon-Jee Yoo, WooCheol Lee, Hyeon-Cheol Kim, Won-Jun Shon, Seung-Ho Baek Restorative Dentistry & Endodontics.2013; 38(3): 154. CrossRef - Review of root canal irrigant delivery techniques and devices
Yeon-Jee Yoo, Su-Jeong Shin, Seung-Ho Baek Journal of Korean Academy of Conservative Dentistry.2011; 36(3): 180. CrossRef
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Clinical study of shade improvement and safety of polymer-based pen type BlancTic Forte whitening agent containing 8.3% Carbamide peroxide
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Jin-Kyung Lee, Sun-Hong Min, Sung-Tae Hong, So-Ram Oh, Shin-Hye Chung, Young-Hye Hwang, Sung-Yeop You, Kwang-Shik Bae, Seung-Ho Baek, Woo-Cheol Lee, Won-Jun Son, Kee-Yeon Kum
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J Korean Acad Conserv Dent 2009;34(2):154-161. Published online March 31, 2009
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DOI: https://doi.org/10.5395/JKACD.2009.34.2.154
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Abstract
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This clinical study evaluated the whitening effect and safety of polymer based-pen type BlancTis Forte (NIBEC) containing 8.3% carbamide peroxide. Twenty volunteers used the BlancTis Forte whitening agent for 2 hours twice a day for 4 weeks. As a control, Whitening Effect Pen (LG) containing 3% hydrogen peroxide was used by 20 volunteers using the same protocol. The change in shade (ΔE*, color difference) was measured using Shadepilot™ (DeguDent) before, during, and after bleaching (2 weeks, 4 weeks, and post-bleaching 4 weeks). A clinical examination for any side effects (tooth hypersensitivity or soft tissue complications) was also performed at each check-up. The following results were obtained.
1. Both the experimental and control groups displayed a noticeable change in shade (ΔE) of over 2. No significant differences were found between the two groups (p > 0.05), implying that the two agents have a similar whitening effect.
2. The whitening effect was mainly due to changes in a and b values rather than in L value (brightness). The experimental group showed a significantly higher change in b value, thus yellow shade, than the control (p < 0.05).
3. None of the participants complained of tooth hypersensitivity or soft tissue complications, confirming the safety of both whitening agents.
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Citations
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- Surface Damage and Bleaching Effect according to the Application Type of Home Tooth Bleaching Applicants
Na-Yeoun Tak, Do-Seon Lim, Hee-Jung Lim, Im-Hee Jung Journal of Dental Hygiene Science.2020; 20(4): 252. CrossRef - Efficacy of a self - applied paint - on whitening gel combined with wrap
Soo-Yeon Kim, Jae-Hyun Ahn, Ji-Young Kim, Jin-Woo Kim, Se-Hee Park, Kyung-Mo Cho Journal of Dental Rehabilitation and Applied Science.2018; 34(3): 175. CrossRef
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Ingredients and cytotoxicity of MTA and 3 kinds of Portland cements
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Seok-Woo Chang, Hyun-Mi Yoo, Dong Sung Park, Tae-Seok Oh, Kwang-Shik Bae
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J Korean Acad Conserv Dent 2008;33(4):369-376. Published online July 31, 2008
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DOI: https://doi.org/10.5395/JKACD.2008.33.4.369
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Abstract
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The aim of this study was to compare the compositions and cytotoxicity of white ProRoot MTA (white mineral trioxide aggregate) and 3 kinds of Portland cements. The elements, simple oxides and phase compositions of white MTA (WMTA), gray Portland cement (GPC), white Portland cement (WPC) and fast setting cement (FSC) were measured by inductively coupled plasma atomic emission spectrometry (ICP-AES), X-ray fluorescence spectrometry (XRF) and X-ray diffractometry (XRD). Agar diffusion test was carried out to evaluate the cytotoxicity of WMTA and 3 kinds of Portland cements.
The results showed that WMTA and WPC contained far less magnesium (Mg), iron (Fe), manganese (Mn), and zinc (Zn) than GPC and FSC. FSC contained far more aluminum oxide (Al2O3) than WMTA, GPC, and WPC. WMTA, GPC, WPC and FSC were composed of main phases, such as tricalcicium silicate (3CaO·SiO2), dicalcium silicate (2CaO·SiO2), tricalcium aluminate (3CaO·Al2O3), and tetracalcium aluminoferrite (4CaO·Al2O3·Fe2O3). The significance of the differences in cellular response between WMTA, GPC, WPC and FSC was statistically analyzed by Kruskal-Wallis Exact test with Bonferroni's correction. The result showed no statistically significant difference (p > 0.05).
WMTA, GPC, WPC and FSC showed similar compositions. However there were notable differences in the content of minor elements, such as aluminum (Al), magnesium, iron, manganese, and zinc. These differences might influence the physical properties of cements.
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Citations
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- Development of Multi-functional Composite Cement with Strength Improvement Using Disposable Waste Masks
Jong-Won Chung, Hyun-Kyoung Yang Journal of Power System Engineering.2022; 26(3): 31. CrossRef - The effects of mineral trioxide aggregate on osteo/odontogenic potential of mesenchymal stem cells: a comprehensive and systematic literature review
Danial Babaki, Sanam Yaghoubi, Maryam M. Matin Biomaterial Investigations in Dentistry.2020; 7(1): 175. CrossRef - Remineralization of demineralized dentin using a dual analog system
Neha Saxena, Stefan Habelitz, Grayson W. Marshall, Laurie B. Gower Orthodontics & Craniofacial Research.2019; 22(S1): 76. CrossRef - Chemical analysis and biological properties of two different formulations of white portland cements
Hany Mohamed Aly Ahmed, Norhayati Luddin, Thirumulu Ponnuraj Kannan, Khairani Idah Mokhtar, Azlina Ahmad Scanning.2016; 38(4): 303. CrossRef - In vitrocytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assay
Sedigheh Khedmat, Somayyeh Dehghan, Jamshid Hadjati, Farimah Masoumi, Mohammad Hossein Nekoofar, Paul Michael Howell Dummer Restorative Dentistry & Endodontics.2014; 39(3): 149. CrossRef - Conservative approach of a symptomatic carious immature permanent tooth using a tricalcium silicate cement (Biodentine): a case report
Cyril Villat, Brigitte Grosgogeat, Dominique Seux, Pierre Farge Restorative Dentistry & Endodontics.2013; 38(4): 258. CrossRef - Chemical characteristics of mineral trioxide aggregate and its hydration reaction
Seok-Woo Chang Restorative Dentistry & Endodontics.2012; 37(4): 188. CrossRef - Physical and chemical properties of experimental mixture of mineral trioxide aggregate and glass ionomer cement
Yu-Na Jeong, So-Young Yang, Bum-Jun Park, Yeong-Joon Park, Yun-Chan Hwang, In-Nam Hwang, Won-Mann Oh Journal of Korean Academy of Conservative Dentistry.2010; 35(5): 344. CrossRef - Biocompatibility of bioaggregate cement on human pulp and periodontal ligament (PDL) derived cells
Choo-Ryung Chung, Euiseong Kim, Su-Jung Shin Journal of Korean Academy of Conservative Dentistry.2010; 35(6): 473. CrossRef - Physical properties of novel composite using Portland cement for retro-filling material
Sang-Jin Lee, Ok-In Cho, Jiwan Yum, Jeong-Kil Park, Bock Hur, Hyeon-Cheol Kim Journal of Korean Academy of Conservative Dentistry.2010; 35(6): 445. CrossRef - A bioactivity study of Portland cement mixed with β-glycerophosphosphate on human pulp cell
Young-Hwan Oh, Young-Joo Jang, Yong-Bum Cho Journal of Korean Academy of Conservative Dentistry.2009; 34(5): 415. CrossRef - Comparison of biocompatibility of four root perforation repair materials
Min-Kyung Kang, In-Ho Bae, Jeong-Tae Koh, Yun-Chan Hwang, In-Nam Hwang, Won-Mann Oh Journal of Korean Academy of Conservative Dentistry.2009; 34(3): 192. CrossRef - Effects of condensation techniques and canal sizes on the microleakage of orthograde MTA apical plug in simulated canals
Deuk-Lim Nam, Jeong-Kil Park, Bock Hur, Hyeon-Cheol Kim Journal of Korean Academy of Conservative Dentistry.2009; 34(3): 208. CrossRef
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Anterior esthetic improvement through orthodontic extrusive remodeling and single-unit implantation in a fractured upper lateral incisor with alveolar bone loss: A case report
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Soo-Youn Hwang, Won-Jun Shon, Young-Chul Han, Kwang-Shik Bae, Seung-Ho Back, WooCheol Lee, Kee-Yeon Kum
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J Korean Acad Conserv Dent 2008;33(1):39-44. Published online January 31, 2008
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DOI: https://doi.org/10.5395/JKACD.2008.33.1.039
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Abstract
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The treatment of esthetic areas with single-tooth implants represents a new challenge for the clinician. In 1993, a modification of the forced eruption technique, called "orthodontic extrusive remodelling," was proposed as a way to augment both soft- and hard-tissue profiles at potential implant sites. This case report describes augmentation of the coronal soft and hard tissues around a fractured maxillary lateral incisor associated with alveolar bone loss, which was achieved by forced orthodontic extrusion before implant placement. Through these procedures we could reconstruct esthetics and function in a hopeless tooth diagnosed with subgingival root fracture by trauma.
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The effect of mineral trioxide aggregate on the production of growth factors and cytokine by human periodontal ligament fibroblasts
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Ji-Yoon Kwon, Sung-Sam Lim, Seung-Ho Baek, Kwang-Shik Bae, Myung-Hoe Kang, Woocheol Lee
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J Korean Acad Conserv Dent 2007;32(3):191-197. Published online May 31, 2007
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DOI: https://doi.org/10.5395/JKACD.2007.32.3.191
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Mineral trioxide aggregate (MTA) would influence healing of periapical tissues by modulating the production of growth factors and cytokines from PDL fibroblasts, however, the studies are insufficient. Therefore, the purpose of this study was to monitor the expression of transforming growth factor-beta1 (TGF-β1), fibroblast growth factor-2 (FGF-2), and interleukin-6 (IL-6) from PDL fibroblasts in the presence of MTA. The human PDL fibroblasts were seeded onto the set MTA or IRM at a level of 1 × 105 cells per unit well, and further incubated for 6, 12, 24, and 48 hours. The levels of TGF-β1, FGF-2, and IL-6 from the supernatant were measured by enzyme-linked immunosorbent assay (ELISA). The data were analyzed using one-way ANOVA. The level of TGF-β1 was down-regulated when the cells were grown in the presence of MTA except at 6 hours. The levels of FGF-2 release were significantly suppressed when PDL fibroblasts were grown in the presence of MTA or IRM at all time intervals (p < 0.05). The expressions of IL-6 from MTA treated cells were comparable to those of untreated control cells throughout the observation periods. We presume that this material inhibits the stimulatory function of growth factors on granulation tissue formation and in turn, it promotes the healing process modulated by other bone-remodeling cells.
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Citations
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- Osteo/odontogenic Differentiation of Human Mesenchymal Stem Cells with Platelet-rich Plasma and Mineral Trioxide Aggregate
Shanthi Vanka, Amit Vanka, Sandeep Kumar Vishwakarma, Manohar K Bhat, Othman Wali, Aleem A Khan The Journal of Contemporary Dental Practice.2019; 20(10): 1171. CrossRef - The effect of several root-end filling materials on MG63 osteoblast-like cells
Jeong-Ho Lee, Won-Jun Shon, WooCheol Lee, Seung-Ho Baek Journal of Korean Academy of Conservative Dentistry.2010; 35(3): 222. CrossRef - Biocompatibility of experimental mixture of mineral trioxide aggregate and glass ionomer cement
Min-Jae Oh, Yu-Na Jeong, In-Ho Bae, So-Young Yang, Bum-Jun Park, Jeong-Tae Koh, Yun-Chan Hwang, In-Nam Hwang, Won-Mann Oh Journal of Korean Academy of Conservative Dentistry.2010; 35(5): 359. CrossRef - Biocompatibility of bioaggregate cement on human pulp and periodontal ligament (PDL) derived cells
Choo-Ryung Chung, Euiseong Kim, Su-Jung Shin Journal of Korean Academy of Conservative Dentistry.2010; 35(6): 473. CrossRef - Effects of condensation techniques and canal sizes on the microleakage of orthograde MTA apical plug in simulated canals
Deuk-Lim Nam, Jeong-Kil Park, Bock Hur, Hyeon-Cheol Kim Journal of Korean Academy of Conservative Dentistry.2009; 34(3): 208. CrossRef
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Measurement of thermal expansion characteristic of root canal filling materials: Gutta-percha and Resilon
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Kyung-A Jeon, In-Bog Lee, Kwang-Shik Bae, Woo-Cheol Lee, Seung-Ho Baek
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J Korean Acad Conserv Dent 2006;31(5):344-351. Published online September 30, 2006
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DOI: https://doi.org/10.5395/JKACD.2006.31.5.344
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The purpose of this study was to evaluate the thermal expansion characteristics of injectable thermoplasticized gutta-perchas and a Resilon. The materials investigated are Obtura gutta-percha, Diadent gutta-percha, E&Q Gutta-percha Bar and Epiphany (Resilon).
The temperature at the heating chamber orifice of an Obtura II syringe and the extruded gutta-percha from the tip of both 23- and 20-gauge needle was determined using a Digital thermometer. A cylindrical ceramic mold was fabricated for thermal expansion test, which was 27 mm long, with an internal bore diameter of 3 mm and an outer diameter of 10 mm. The mold was filled with each experimental material and barrel ends were closed with two ceramic plunger. The samples in ceramic molds were heated in a dilatometer over the temperature range from 25℃ to 75℃. From the change of specimen length as a function of temperature, the coefficients of thermal expansion were determined.
There was no statistical difference between four materials in the thermal expansion in the range from 35℃ to 55℃ (p > 0.05). However, Obtura Gutta-percha showed smaller thermal expansion than Diadent and Metadent ones from 35℃ to 75℃ (p < 0.05). The thermal expansion of Epiphany was similar to those of the other gutta-percha groups.
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Cytotoxicity and genotoxicity of newly developed calcium phosphate-based root canal sealers
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Hee-Jung Kim, Seung-Ho Baek, Kwang-Shik Bae
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J Korean Acad Conserv Dent 2006;31(1):36-49. Published online January 31, 2006
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DOI: https://doi.org/10.5395/JKACD.2006.31.1.036
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Abstract
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The purpose of this study was to compare the cytotoxicity by MTT test and genotoxicity by Ames test of new calcium phosphate-based root canal sealers (CAPSEAL I, CAPSEAL II) with commercially available resin-based sealers (AH 26, AH Plus), zinc oxide eugenol-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT), calcium hydroxide-based sealer (Sealapex), and tricalcium phosphate based sealers (Sankin Apatite Root Canal Sealer I, II, III).
According to this study, the results were as follows:
The extracts of freshly mixed group showed higher toxicity than those of 24 h set group in MTT assay (p < 0.001).
CAPSEAL I and CAPSEAL II were less cytotoxic than AH 26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT, Sealapex and SARCS II in freshly mixed group (p < 0.01).
AH 26 in freshly mixed group showed mutagenicity to TA98 and TA100 with and without S9 mix and AH Plus extracts also were mutagenic to TA100 with and without S9 mix.
Tubliseal EWT, Pulp Canal Sealer EWT and Sealapex in freshly mixed group were mutagenic to TA100 with S9 mix.
Among those of 24 h set groups, the extracts of SARCS II were mutagenic to TA98 with and without S9 mix and AH 26 showed mutagenic effects to TA98 with S9 mix.
No mutagenic effect of CAPSEAL I and CAPSEAL II was detected.
There is no statistically significant difference between CAPSEAL I and CAPSEAL II at MTT assay and Ames test in both freshly mixed group and 24 h set group.
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- In vitrocytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assay
Sedigheh Khedmat, Somayyeh Dehghan, Jamshid Hadjati, Farimah Masoumi, Mohammad Hossein Nekoofar, Paul Michael Howell Dummer Restorative Dentistry & Endodontics.2014; 39(3): 149. CrossRef - A comparative evaluation of cytotoxicity of root canal sealers: anin vitrostudy
Gautam Pyarelal Badole, Manjusha Madhukar Warhadpande, Ganesh Kothiramji Meshram, Rakesh Namdeoraoji Bahadure, Shubha Gopal Tawani, Gopal Tawani, Shital Gautam Badole Restorative Dentistry & Endodontics.2013; 38(4): 204. CrossRef - Comparison of biocompatibility of four root perforation repair materials
Min-Kyung Kang, In-Ho Bae, Jeong-Tae Koh, Yun-Chan Hwang, In-Nam Hwang, Won-Mann Oh Journal of Korean Academy of Conservative Dentistry.2009; 34(3): 192. CrossRef
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Cytotoxicity of resin-based root canal sealer, adseal
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Hee-Jung Kim, Seung-Ho Baek, Woo-Cheol Lee, Han-Soo Park, Kwang-Shik Bae
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J Korean Acad Conserv Dent 2004;29(6):498-503. Published online November 30, 2004
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DOI: https://doi.org/10.5395/JKACD.2004.29.6.498
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Abstract
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The properties of ideal root canal sealers include the ability of sealing the total root canal system and no toxic effects to periradicular tissues. Cytotoxicity test using cell culture is a common screening method for evaluation of the biocompatibility of root canal sealers. The purpose of this study was to investigate the cytotoxic effect of newly developed resin-based sealer (Adseal 1, 2, and 3) comparing with those commercial resin-based sealers (AH26 and AH Plus), ZOE-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT) and calcium hydroxide based sealer (Sealapex). An indirect contact test of cytotoxicity by agar diffusion was performed according to the international standard ISO 10993-5. L929 fibroblast cells were incubated at 37℃ in humidified 5% CO2-containing air atmosphere. The freshly mixed test materials were inserted into glass rings of internal diameter 5 mm and height 5 mm placed on the agar. After the 24 hrs incubation period, the decolorization zones around the test materials were assessed using an inverted microscope with a calibrated screen. A Decolorization Index was determined for each specimen. Adseal 1, 2, and 3 did not exert any cytotoxic effects, whereas AH26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT, and Sealapex produced mild cytotoxicity.
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Citations
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- Cytotoxicity Comparison of Sure-seal root and Adseal Sealers on mouse fibroblast Cells:Invitro study
Azam haddadikohsar, Mohammad shokrzade, Marjan Fallah, Fatemeh Shakeri journal of research in dental sciences.2024; 21(1): 46. CrossRef - Antimicrobial efficacy of Kerr pulp canal sealer (EWT) in combination with 10% amoxicillin on Enterococcus faecalis: A confocal laser scanning microscopic study
Madhureema De Sarkar, Kundabala Mala, Suchitra Shenoy Mala, Shama Prasada Kabekkodu, Srikant Natarajan, Neeta Shetty, Priyanka Madhav Kamath, Manuel Thomas F1000Research.2023; 12: 725. CrossRef - Antimicrobial efficacy of Kerr pulp canal sealer (EWT) in combination with 10% amoxicillin on Enterococcus faecalis: A confocal laser scanning microscopic study
Madhureema De Sarkar, Kundabala Mala, Suchitra Shenoy Mala, Shama Prasada Kabekkodu, Srikant Natarajan, Neeta Shetty, Priyanka Madhav Kamath, Manuel Thomas F1000Research.2023; 12: 725. CrossRef - Cytotoxicity and Genotoxicity of Epoxy Resin-Based Root Canal Sealers before and after Setting Procedures
Mijoo Kim, Marc Hayashi, Bo Yu, Thomas K. Lee, Reuben H. Kim, Deuk-won Jo Life.2022; 12(6): 847. CrossRef - Biocompatibility and Bioactivity of Four Different Root Canal Sealers in Osteoblastic Cell Line MC3T3-El
Nu-Ri Jun, Sun-Kyung Lee, Sang-Im Lee Journal of Dental Hygiene Science.2021; 21(4): 243. CrossRef
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The effect of Treponema denticola immunoinhibitory protein on cytokine expression in T cells
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Sang-Yup Lee, Won-Jun Shon, WooCheol Lee, Seung-Ho Baek, Kwang-Shik Bae, SungSam Lim
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J Korean Acad Conserv Dent 2004;29(5):479-484. Published online September 30, 2004
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DOI: https://doi.org/10.5395/JKACD.2004.29.5.479
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Abstract
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Immunoinhibitory protein extracted from sonicated Treponema denticola have been shown to suppress cell cycle progression of human lymphocytes. To study in detail about the effect of this microorganism on the function of lymphocytes, we investigated the levels of Interleukin-2 (IL-2) and Interleukin-4 (IL-4) production by T lymphocytes before and after the addition of 12.5 µg/ml T. denticola sonicated extracts. In this study, levels of IL-2 and IL-4 produced from T cells pretreated with sonicated extracts were evaluated by using the quantitative sandwich enzyme immunoassay technique. In response to phytohemagglutinin (PHA) stimulation, T cell produced increased levels of IL-2 and IL-4. However, the expressions of both cytokines were significantly inhibited when PHA activated-T cells were pre-exposed to sonicated T. denticola extracts (p < 0.05). These findings suggest that the T. denticola sonicated extracts induced-immunosuppression in Th1 and Th2 cell functions could be a part of the pathogenic mechanism of the endodontic failure associated with this microorganism.
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- TLR5 activation induces expression of the pro‐inflammatory mediator Urokinase Plasminogen Activator via NF‐κB and MAPK signalling pathways in human dental pulp cells
H.‐S. Hwang, J.‐W. Kim, S.‐H. Oh, J. H. Song, J.‐W. Yang, Y. Zang, Y.‐H. Kim, S.‐E. Lee, Y.‐C. Hwang, J.‐T. Koh International Endodontic Journal.2019; 52(10): 1479. CrossRef
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Evaluation of the cytotoxicity of calcium phosphate root canal sealers
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Jee-Hyun Lee, Seung-Ho Baek, Kwang-Shik Bae
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J Korean Acad Conserv Dent 2003;28(4):295-302. Published online July 31, 2003
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DOI: https://doi.org/10.5395/JKACD.2003.28.4.295
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- BIOCERAMIC-BASED ROOT CANAL SEALERS
L Somolová, Z Zapletalová, M Rosa, B Novotná, I Voborná, Y Morozova Česká stomatologie a praktické zubní lékařství.2021; 121(4): 116. CrossRef - In vitrocytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assay
Sedigheh Khedmat, Somayyeh Dehghan, Jamshid Hadjati, Farimah Masoumi, Mohammad Hossein Nekoofar, Paul Michael Howell Dummer Restorative Dentistry & Endodontics.2014; 39(3): 149. CrossRef - Chronic maxillary sinusitis caused by root canal overfilling of Calcipex II
Jin-Woo Kim, Kyung-Mo Cho, Se-Hee Park, Soh-Ra Park, Sang-Shin Lee, Suk-Keun Lee Restorative Dentistry & Endodontics.2014; 39(1): 63. CrossRef
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In vivo study on the biocompatibility of new resin-based root canal sealers
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Yong-Beom Kim, Seung-Ho Baek, Kwang-Shik Bae
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J Korean Acad Conserv Dent 2002;27(2):122-134. Published online March 31, 2002
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DOI: https://doi.org/10.5395/JKACD.2002.27.2.122
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- Effect of Different Sealers on the Cytocompatibility and Osteogenic Potential of Human Periodontal Ligament Stem Cells: An In Vitro Study
Shehabeldin Saber, Shereen Raafat, Mohamed Elashiry, Ahmed El-Banna, Edgar Schäfer Journal of Clinical Medicine.2023; 12(6): 2344. CrossRef - Cytotoxicity of resin-based root canal sealer, adseal
Hee-Jung Kim, Seung-Ho Baek, Woo-Cheol Lee, Han-Soo Park, Kwang-Shik Bae Journal of Korean Academy of Conservative Dentistry.2004; 29(6): 498. CrossRef
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