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- Basic Research Matrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth
- Su-Jung Shin, DDS, MS1, Woocheol Lee, DDS, PhD2, Jae-Il Lee, DDS, MDS, PhD3, Seung-Ho Baek, DDS, PhD2, Kee-Yeon Kum, DDS, PhD2, Won-Jun Shon, DDS, PhD2, Kwang-Shik Bae, DDS, MDS, PhD2
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Journal of Korean Academy of Conservative Dentistry 2011;36(3):196-202.
DOI: https://doi.org/10.5395/JKACD.2011.36.3.196
Published online: May 31, 2011
1Department of Conservative Dentistry, College of Dentistry, Yonsei University, Seoul, Korea.
2Department of Conservative Dentistry, Dental Research Institute, Seoul, Korea.
3Department of Oral Pathology, School of Dentistry, Seoul National University, Seoul, Korea.
- Correspondence to Kwang-Shik Bae, DDS, MDS, PhD. Professor, Department of Conservative Dentistry, Seoul National University School of Dentistry and Dental Research Institute, 28-2 Yeongeon-dong, Jongro-gu, Seoul, Korea 110-749. TEL, +82-2-2072-2651; FAX, +82-2-764-3859; baeks@snu.ac.kr
• Received: March 10, 2011 • Revised: April 4, 2011 • Accepted: April 4, 2011
Copyright © 2011 Korean Academy of Conservative Dentistry
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Abstract
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Objectives The aim of this study was to investigate levels of matrix metalloproteinase-8 (MMP-8) and substance P (SP) in root canal exudates during root canal treatment (RCT) of nonvital, painful teeth.
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Materials and Methods Patients scheduled for nonsurgical RCT were prospectively selected; the study was performed after obtaining informed consent from the patients and was approved by the Institutional Review Board for Clinical Research of Gangnam Severance Hospital, Yonsei University (3-2008-0118). Canal exudates samples were collected using sterilized paper points from teeth scheduled for RCT across three different time periods. MMP-8 and SP levels were measured using enzyme-linked immunosorbent assay (ELISA). Data were analyzed using a mixed model analysis and the Pearson correlation analysis (p < 0.05).
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Results MMP-8 and SP levels in GCF were decreased during RCT (p < 0.0001), and they showed a weak positive correlation to each other (p < 0.05). Patients'subjective pain levels and the response from percussion test were significantly related to SP level.
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Conclusions This study demonstrated that periradicular inflammation endodontic origin can elevate SP and MMP-8 levels in root canal exudates. Interestingly, SP level of canal exudates showed a possibility of being used as an indicator of pain due to periapical pathosis.
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XML DownloadMatrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth
Figure 1
The amount of MMP-8 and SP from individual samples.
Figure 2
Corrected estimates and standard errors of MMP-8 and SP levels in the experimental group. A mixed model was used for adjusting patients' pain level.
Figure 3
Scatter plot of MMP-8 and SP at each period. (a) At first visit, (b) At second visit, (c) At third visit.
Figure 1
Figure 2
Figure 3
Matrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth
