Restorative Dentistry & Endodontics

Research Articles

Bone repair in defects filled with AH Plus sealer and different concentrations of MTA: a study in rat tibiae: Jessica Emanuella Rocha Paz, Priscila Oliveira Costa, Albert Alexandre Costa Souza, Ingrid Macedo de Oliveira, Lucas Fernandes Falcão, Carlos Alberto Monteiro Falcão, Maria Ângela Area Leão Ferraz, Lucielma Salmito Soares Pinto; Restor Dent Endod 2021;46(4):e48. Published online September 2, 2021; DOI: https://doi.org/10.5395/rde.2021.46.e48

Objectives

This study aimed to evaluate the effects on bone repair of different concentrations of mineral trioxide aggregate (MTA) added to AH Plus.

Materials and Methods

Bone tissue reactions were evaluated in 30 rats (Rattus norvegicus) after 7 and 30 days. In the AH + MTA10, AH + MTA20, and AH + MTA30 groups, defects in the tibiae were filled with AH Plus with MTA in proportions of 10%, 20% and 30%, respectively; in the MTA-FILL group, MTA Fillapex was used; and in the control group, no sealer was used. The samples were histologically analyzed to assess bone union and maturation. The Kruskal-Wallis and Mann-Whitney tests were performed for multiple pairwise comparisons (p ≤ 0.05).

Results

At the 7-day time point, AH + MTA10 was superior to MTA-FILL with respect to bone union, and AH + MTA20 was superior to MTA-FILL with respect to bone maturity (p < 0.05). At the 30-day time point, both the AH + MTA10 and AH + MTA20 experimental sealers were superior not only to MTA-FILL, but also to AH + MTA30 with respect to both parameters (p < 0.05). The results of the AH + MTA10 and AH + MTA20 groups were superior to those of the control group for both parameters and experimental time points (p < 0.05).

Conclusions

The results suggest the potential benefit of using a combination of these materials in situations requiring bone repair.

Citations

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Analysis of the cytotoxicity and bioactivity of CeraSeal, BioRoot™ and AH Plus® sealers in pre-osteoblast lineage cells
Luciano Aparecido de Almeida-Junior, Giuliana de Campos Chaves Lamarque, Henry Herrera, Maya Fernanda Manfrin Arnez, Francine Lorencetti-Silva, Raquel Assed Bezerra Silva, Léa Assed Bezerra Silva, Francisco Wanderley Garcia Paula-Silva
BMC Oral Health.2024;[Epub] CrossRef
A Review of the research methods and progress of biocompatibility evaluation of root canal sealers
Xiliang Yang, Tianxia Zheng, Nuoya Yang, Zihan Yin, Wuliang Wang, Yuhong Bai
Australian Endodontic Journal.2023; 49(S1): 508. CrossRef
Effect of Vitapex Combined with AH-Plus Paste on Inflammation in Middle-Aged and Elderly Patients with Periodontal-Endodontic Disease
Rong Hu, Fulan Zhang, Xiangyu Guo, Youren Jing, Xiaowan Lin, Liping Tian, Min Tang
Computational and Mathematical Methods in Medicine.2022; 2022: 1. CrossRef

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Effects of radiation therapy on the dislocation resistance of root canal sealers applied to dentin and the sealer-dentin interface: a pilot study: Pallavi Yaduka, Rubi Kataki, Debosmita Roy, Lima Das, Shachindra Goswami; Restor Dent Endod 2021;46(2):e22. Published online March 29, 2021; DOI: https://doi.org/10.5395/rde.2021.46.e22

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Objectives

This study evaluated and compared the effects of radiation therapy on the dislocation resistance of AH Plus and BioRoot RCS applied to dentin and the sealer-dentin interface.

Materials and Methods

Thirty single-rooted teeth were randomly assigned to 2 groups (n = 15 each): AH Plus (Dentsply DeTrey) and BioRoot RCS (Septodont). Each group was subdivided into control and experimental groups. The experimental group was subjected to a total radiation dose of 60 Gy. The root canals of all samples were cleaned, shaped, and obturated using the single-cone technique. Dentin slices (1 mm) were sectioned from each root third for the push-out test and scanning electron microscopy (SEM) was done to examine the sealer-dentin interface. The failure mode was determined using stereomicroscopy. Bond strength data were analyzed by the independent t-test, 1-way analysis of variance, and the Tukey post hoc test (α = 0.05).

Results

Significantly lower bond strength was observed in irradiated teeth than non-irradiated teeth in the AH Plus group (p < 0.05). The BioRoot RCS group showed no significant reduction in bond strength after irradiation (p > 0.05) and showed a higher post-irradiation bond strength (209.92 ± 172.26 MPa) than the AH Plus group. SEM revealed slightly larger gap-containing regions in irradiated specimens from both groups.

Conclusions

The dislocation resistance of BioRoot RCS was not significantly changed by irradiation and was higher than that of AH Plus. BioRoot RCS may be the sealer of choice for root canal treatment in patients undergoing radiation therapy.

Citations

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Impact of radiation therapy regimen on the dislodgement resistance of endodontic sealers: A micro push-out test
Marcos Testa Magoga, Rafaela Lourdes de Sousa, Luiz Carlos Lima Dias-Junior, Rayssa Sabino-Silva, Mariana Comparotto Minamisako, Paulo Marcelo Rodrigues, Vicente Ribeiro Netto, Ricardo Machado, Cleonice da Silveira Teixeira, Lucas da Fonseca Roberti Garci
International Journal of Adhesion and Adhesives.2025; 136: 103894. CrossRef
Evaluation of the root dentin bond strength and intratubular biomineralization of a premixed calcium aluminate-based hydraulic bioceramic endodontic sealer
Yu-Na Lee, Min-Kyeong Kim, Hee-Jin Kim, Mi-Kyung Yu, Kwang-Won Lee, Kyung-San Min
Journal of Oral Science.2024; 66(2): 96. CrossRef
Effects of radiotherapy dose and application time on the load-to-failure values of teeth filled with different sealers
Ozgun Gulderen, Esma Saricam, Sedef Gökhan Açikgöz, Yılmaz Tezcan
BMC Oral Health.2024;[Epub] CrossRef
Ultrasonic activation of the endodontic sealer enhances its intratubular penetration and bond strength to irradiated root dentin
Luana Duart Jordani, Amanda Freitas da Rosa, Luiz Carlos de Lima Dias-Junior, Julia Menezes Savaris, Mariana Comparotto Minamisako, Luciano Roberto da Silva, Marcio Toshio Umeda Takashima, Eduardo Antunes Bortoluzzi, Cleonice da Silveira Teixeira, Lucas d
Odontology.2024; 112(3): 917. CrossRef
Effect of the timing of primary endodontic treatment and dosage of radiation therapy on the filling material removal
Bruna Venzke Fischer, Luiz Carlos de Lima Dias‐Junior, Mariana Comparotto Minamisako, Cristiane Maria Almeida, Luciano Roberto da Silva, Eduardo Antunes Bortoluzzi, Cleonice da Silveira Teixeira, Lucas da Fonseca Roberti Garcia
Australian Endodontic Journal.2024; 50(2): 321. CrossRef
Does radiation therapy affect adhesion of tricalcium silicate cements to root dentin?
Lochan KHULLAR, Nidambur Vasudev BALLAL, Tan Fırat EYÜBOĞLU, Mutlu ÖZCAN
Journal of Applied Oral Science.2023;[Epub] CrossRef
Effect of the timing of radiation therapy on the push‐out strength of resin cement to root dentine
Patrícia da Agostim Cancelier, Renata Gondo Machado, Júlia Menezes Savaris, Eduardo Antunes Bortoluzzi, Cleonice da Silveira Teixeira, Mariana Comparotto Minamisako, Paulo Marcelo Rodrigues, Vicente Ribeiro Netto, Kamile Leonardi Dutra‐Horstmann, Lucas da
Australian Endodontic Journal.2023; 49(S1): 122. CrossRef
Influence of irrigation and laser assisted root canal disinfection protocols on dislocation resistance of a bioceramic sealer
Ivona Bago, Ana Sandrić, Katarina Beljic-Ivanovic, Boris Pažin
Photodiagnosis and Photodynamic Therapy.2022; 40: 103067. CrossRef
Influence of 2% chlorhexidine on the dislodgement resistance of AH plus, bioroot RCS, and GuttaFlow 2 sealer to dentin and sealer-dentin interface
Debosmita Roy, Rubi Kataki, Lima Das, Khushboo Jain
Journal of Conservative Dentistry.2022; 25(6): 642. CrossRef

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Flow characteristics and alkalinity of novel bioceramic root canal sealers: Anastasios Katakidis, Konstantinos Sidiropoulos, Elisabeth Koulaouzidou, Christos Gogos, Nikolaos Economides; Restor Dent Endod 2020;45(4):e42. Published online August 18, 2020; DOI: https://doi.org/10.5395/rde.2020.45.e42

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Objective

This study aimed to examine the physical properties (pH and flow) of 2 novel bioceramic sealers.

Materials and Methods

The tested sealers were a calcium hydroxide sealer (Sealapex) and 2 bioceramic sealers (BioRoot RCS and TotalFill BC Sealer). Flow measurements were conducted according to ISO 6876/2012, with a press method of 0.05 mL of sealer. The pH of fresh samples was tested immediately after manipulation, while set samples were stored for 3 times the recommended setting time. The predetermined time intervals ranged from 3 minutes to 24 hours for fresh samples and from 10 minutes to 7 days and 4 weeks for the set samples. Analysis of variance was performed, with p = 0.05 considered indicating significance.

Results

The mean flow values were 26.99 mm for BioRoot, 28.19 for Sealapex, and 30.8 mm for TotalFill BC Sealer, satisfying the ISO standard. In the set samples, BioRoot RCS had higher pH values at 24 hours to 1 week after immersion in distilled water. At 2 weeks, both bioceramic sealers had similar pH values, greater than that of Sealapex. In the fresh samples, the bioceramic sealers had significantly higher initial pH values than Sealapex (p < 0.05). At 24 hours post-immersion, all sealers showed an alkaline pH, with the highest pH observed for TotalFill.

Conclusions

The TotalFill BC Sealer demonstrated the highest flow. The bioceramic sealers initially presented higher alkaline activity than the polymeric calcium hydroxide sealer. However, at 3 and 4 weeks post-immersion, all sealers had similar pH values.

Citations

Citations to this article as recorded by

Physicochemical properties of AH plus bioceramic sealer, Bio-C Sealer, and ADseal root canal sealer
Tamer M. Hamdy, Manar M. Galal, Amira Galal Ismail, Shehabeldin Saber
Head & Face Medicine.2024;[Epub] CrossRef
Characterization and Assessment of Physical Properties of 3 Single Syringe Hydraulic Cement–based Sealers
Veksina Raman, Josette Camilleri
Journal of Endodontics.2024; 50(3): 381. CrossRef
The Impact of Silver Nanoparticles on Dentinal Tubule Penetration of Endodontic Bioceramic Sealer
Sundus Bukhary, Sarah Alkahtany, Amal Almohaimede, Nourah Alkhayatt, Shahad Alsulaiman, Salma Alohali
Applied Sciences.2024; 14(24): 11639. CrossRef
Influence of root canal moisture on the penetration of TotalFill bioceramic sealer into the dentinal tubules: A confocal laser scanning microscopy study
Archika M Singh, Tarek M Elsewify, Walid S El-Sayed, Husam H Nuawafleh, Ranya F Elemam, Bassem M Eid
Saudi Endodontic Journal.2024; 14(2): 187. CrossRef
Unusual Canal Morphology in Mandibular Premolars With Two Distal and One Mesial Canal: A Case Series
Jinesh A, Sanjana Jayakumar Nair, Saurabh Gupta, Harsh Chansoria, Gaurav Rawat
Cureus.2024;[Epub] CrossRef
A scientometric, bibliometric, and thematic map analysis of hydraulic calcium silicate root canal sealers
Anastasios Katakidis, Konstantinos Kodonas, Anastasia Fardi, Christos Gogos
Restorative Dentistry & Endodontics.2023;[Epub] CrossRef
Thermal, chemical and physical analysis of VDW.1Seal, Fill Root ST, and ADseal root canal sealers
Shehabeldin Saber, Manar M. Galal, Amira Galal Ismail, Tamer M. Hamdy
Scientific Reports.2023;[Epub] CrossRef
α-tricalcium phosphate/fluorapatite-based cement - promising dental root canal filling material
Abdul Kazuz, Zeljko Radovanovic, Djordje Veljovic, Vesna Kojic, Dimitar Jakimov, Tamara Vlajic-Tovilovic, Vesna Miletic, Rada Petrovic, Djordje Janackovic
Processing and Application of Ceramics.2022; 16(1): 22. CrossRef

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A comparative evaluation of cytotoxicity of root canal sealers: an in vitro study: Gautam Pyarelal Badole, Manjusha Madhukar Warhadpande, Ganesh Kothiramji Meshram, Rakesh Namdeoraoji Bahadure, Shubha Gopal Tawani, Gopal Tawani, Shital Gautam Badole; Restor Dent Endod 2013;38(4):204-209. Published online November 12, 2013; DOI: https://doi.org/10.5395/rde.2013.38.4.204

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Objectives

The objective of this in vitro study was to evaluate and compare the cytotoxicity of four different root canal sealers i.e. Apexit Plus (Ivoclar Vivadent), Endomethasone N (Septodont), AH-26 (Dentsply) and Pulpdent Root Canal Sealer (Pulpdent), on a mouse fibroblast cell line (L929).

Materials and Methods

Thirty two discs for each sealer (5 mm in diameter and 2 mm in height) were fabricated in Teflon mould. The sealer extraction was made in cell culture medium (Dulbecco's Modified Eagle's Medium, DMEM) using the ratio 1.25 cm²/mL between the surface of the sealer samples and the volume of medium in a shaker incubator. Extraction of each sealer was obtained at 24 hr, 7th day, 14th day, and one month of interval. These extracts were incubated with L929 cell line and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was done. Two-way ANOVA for interaction effects between sealer and time and Post-hoc multiple comparison using Tukey's test across all the 16 different groups were used for statistical analysis.

Results

Apexit Plus root canal sealer was significantly less toxic than other sealers (p < 0.05) and showed higher cellular growth than control. Endomethasone N showed mild cytotoxicity. AH-26 showed severe toxicity which became mild after one month while Pulpdent Root Canal Sealer showed severe to moderate toxicity.

Conclusions

Apexit Plus was relatively biocompatible sealer as compared to other three sealers which were cytotoxic at their initial stages, however, they became biocompatible with time.

Citations

Citations to this article as recorded by

The Evolution of In Vitro Toxicity Assessment Methods for Oral Cavity Tissues—From 2D Cell Cultures to Organ-on-a-Chip
Alexandra Jităreanu, Luminița Agoroaei, Ioana-Cezara Caba, Florina-Daniela Cojocaru, Liliana Vereștiuc, Mădălina Vieriu, Ioana Mârțu
Toxics.2025; 13(3): 195. CrossRef
Comparative analysis of the inflammatory response of human gingival fibroblasts to NeoSEALER Flo and CeraSeal bioceramic sealers: an in vitro study
Sarah Salah Gaafar, Abdel Rahman O. El Mekkawi, Rehab Ali Farag, Mohamed H. A. Gadelmawla, Ahmad Mostafa Hussein Mohamad Hussein, Mohamed Sayed, Mohammad Rayyan, Doaa Gamal AbdelMouez Basta
BMC Oral Health.2025;[Epub] CrossRef
Cytotoxicity and Cell Viability of Two Bioactive Root Canal Sealers, Mineral Trioxide Aggregate, and BioRoot Root Canal Sealer: An In Vitro Study
Emmanuel Samson, Lata B Gangurde, Jaiprakash R Rathod, Pradnya S Jadhav, Sangeeta Ambhore, Pranav S Jadhav
CODS - Journal of Dentistry.2023; 14(2): 57. CrossRef
Human Gingival Fibroblasts Response to Different Endodontic Sealers: An In Vitro Study
Rita Noites, Inês Tavares, Miguel Cardoso, Isabel M. Carreira, Maria Bartolomeu, Ana S. Duarte, Ilda P. Ribeiro
Applied Sciences.2023; 13(19): 10976. CrossRef
Antibacterial and Cytotoxicity of Root Canal Sealer with the Addition of Chitosan Nanoparticle at Various Concentrations
Diatri Nari Ratih, Ema Mulyawati, Rika Kurnia Santi, Yulita Kristanti
European Journal of Dentistry.2023; 17(02): 398. CrossRef
Transforaminal and systemic diffusion of an active agent from a zinc oxide eugenol-based endodontic sealer containing hydrocortisone—in an in vivo model
Davy Aubeux, Anne Valot-Salengro, Gaelle Gautier, Arnaud Malet, Fabienne Pérez
Clinical Oral Investigations.2020; 24(12): 4395. CrossRef
PEGylated curcumin-loaded nanofibrous mats with controlled burst release through bead knot-on-spring design
Mahdi Saeed, Hamid Mirzadeh, Mojgan Zandi, Jalal Barzin
Progress in Biomaterials.2020; 9(4): 175. CrossRef
A New Calcium Silicate-Based Root Canal Dressing: Physical and Chemical Properties, Cytotoxicity and Dentinal Tubule Penetration
Natália Villa, Vanessa Valgas Dos Santos, Ubirajara Maciel da Costa, Aline Teixeira Mendes, Pedro Henrique Marks Duarte, Ricardo Abreu da Rosa, Jefferson Ricardo Pereira, Marcus Vinícius Reis Só
Brazilian Dental Journal.2020; 31(6): 598. CrossRef
Evaluation of the Antimicrobial Efficacy of Herbal Extracts Added to Root Canal Sealers of Different Bases: An In Vitro Study
Abhay M Tripathi, Minarani T Devi, Sonali K Kalra, Ujjala Ghoshal
International Journal of Clinical Pediatric Dentistry.2019; 12(5): 398. CrossRef
Endodontic-related inferior alveolar nerve injuries: A review and a therapeutic flow chart
R. Castro, M. Guivarc'h, J.M. Foletti, J.H. Catherine, C. Chossegros, L. Guyot
Journal of Stomatology, Oral and Maxillofacial Surgery.2018; 119(5): 412. CrossRef
Comparison between direct contact and extract exposure methods for PFO cytotoxicity evaluation
Girish K. Srivastava, Maria L. Alonso-Alonso, Ivan Fernandez-Bueno, Maria T. Garcia-Gutierrez, Fernando Rull, Jesús Medina, Rosa M. Coco, J. Carlos Pastor
Scientific Reports.2018;[Epub] CrossRef
Novel endodontic sealers induce cell cytotoxicity and apoptosis in a dose-dependent behavior and favorable response in mice subcutaneous tissue
L. A. B. Silva, L. U. Azevedo, A. Consolaro, F. Barnett, Y. Xu, R. A. Battaglino, P. S. Cañadas, Katharina Morant Holanda de Oliveira, R. A. B. Silva
Clinical Oral Investigations.2017; 21(9): 2851. CrossRef
Designing and fabrication of curcumin loaded PCL/PVA multi-layer nanofibrous electrospun structures as active wound dressing
Seyed Mahdi Saeed, Hamid Mirzadeh, Mojgan Zandi, Jalal Barzin
Progress in Biomaterials.2017; 6(1-2): 39. CrossRef

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