Restorative Dentistry & Endodontics

Volume 39 (3); August 2014

Research Articles

In vitro cytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assay: Sedigheh Khedmat, Somayyeh Dehghan, Jamshid Hadjati, Farimah Masoumi, Mohammad Hossein Nekoofar, Paul Michael Howell Dummer; Restor Dent Endod 2014;39(3):149-154. Published online April 30, 2014; DOI: https://doi.org/10.5395/rde.2014.39.3.149

Objectives

This study was performed to evaluate the cytotoxicity of four calcium silicate-based endodontic cements at different storage times after mixing.

Materials and Methods

Capillary tubes were filled with Biodentine (Septodont), Calcium Enriched Mixture (CEM cement, BioniqueDent), Tech Biosealer Endo (Tech Biosealer) and ProRoot MTA (Dentsply Tulsa Dental). Empty tubes and tubes containing Dycal were used as negative and positive control groups respectively. Filled capillary tubes were kept in 0.2 mL microtubes and incubated at 37℃. Each material was divided into 3 groups for testing at intervals of 24 hr, 7 day and 28 day after mixing. Human monocytes were isolated from peripheral blood mononuclear cells and cocultered with 24 hr, 7 day and 28 day samples of different materials for 24 and 48 hr. Cell viability was evaluated using an MTT assay.

Results

In all groups, the viability of monocytes significantly improved with increasing storage time regardless of the incubation time (p < 0.001). After 24 hr of incubation, there was no significant difference between the materials regarding monocyte viability. However, at 48 hr of incubation, ProRoot MTA and Biodentine were less cytotoxic than CEM cement and Biosealer (p < 0.01).

Conclusions

Biodentine and ProRoot MTA had similar biocompatibility. Mixing ProRoot MTA with PBS in place of distilled water had no effect on its biocompatibility. Biosealer and CEM cement after 48 hr of incubation were significantly more cytotoxic to on monocyte cells compared to ProRoot MTA and Biodentine.

Citations

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Comprehensive review of composition, properties, clinical applications, and future perspectives of calcium-enriched mixture (CEM) cement: a systematic analysis
Saeed Asgary, Mahtab Aram, Mahta Fazlyab
BioMedical Engineering OnLine.2024;[Epub] CrossRef
Biocompatibility of mineral trioxide aggregate and biodentine as root-end filling materials: an in vivo study
Mohamed Nabeel, Ashraf M. Abu-Seida, Abeer A. Elgendy, Hossam M. Tawfik
Scientific Reports.2024;[Epub] CrossRef
Apoptotic effects of biodentine, calcium-enriched mixture (CEM) cement, ferric sulfate, and mineral trioxide aggregate (MTA) on human mesenchymal stem cells isolated from the human pulp of exfoliated deciduous teeth
Bahareh NAZEMI SALMAN, Mahshid MOHEBBI RAD, Ehsan SABURI
Minerva Dental and Oral Science.2024;[Epub] CrossRef
Evaluation of the Effects of Retro-Cavity Preconditioning with or Without Ethylenediaminetetraacetic Acid on Root Surface pH and Dislodgement Resistance of NeoMTA2 and Mineral Trioxide Aggregate Flow Retro-Fills: An Ex Vivo Investigation
Sedigheh Khedmat, Seyyed Ali Abaee, Hadi Assadian, Antonio Signore, Stefano Benedicenti
Journal of Functional Biomaterials.2024; 16(1): 3. CrossRef
Bone morphogenetic proteins in biomineralization of two endodontic restorative cements
Tamara A. Souza, Mirna M. Bezerra, Paulo G. B. Silva, José J. N. Costa, Rayssa F. L. A. Carneiro, Janice O. F. Barcelos, Bruno C. Vasconcelos, Hellíada V. Chaves
Journal of Biomedical Materials Research Part B: Applied Biomaterials.2021; 109(3): 348. CrossRef
Cytotoxicity and Bioactivity of Mineral Trioxide Aggregate and Bioactive Endodontic Type Cements: A Systematic Review
Uma Dixit, Rucha Shivajirao Bhise Patil, Rupanshi Parekh
International Journal of Clinical Pediatric Dentistry.2021; 14(1): 30. CrossRef
Physicochemical and Biological Properties of Mg-Doped Calcium Silicate Endodontic Cement
Kyung-Hyeon Yoo, Yong-Il Kim, Seog-Young Yoon
Materials.2021; 14(8): 1843. CrossRef
Comparative evaluation of the effect of cold ceramic and MTA-Angelus on cell viability, attachment and differentiation of dental pulp stem cells and periodontal ligament fibroblasts: an in vitro study
Sedigheh Khedmat, Pegah Sarraf, Ehsan Seyedjafari, Parisa Sanaei-rad, Faranak Noori
BMC Oral Health.2021;[Epub] CrossRef
Cytotoxicity of universal dental adhesive systems: Assessment in vitro assays on human gingival fibroblasts
Stefano Pagano, Guido Lombardo, Stefania Balloni, Maria Bodo, Stefano Cianetti, Antonella Barbati, Azadeh Montaseri, Lorella Marinucci
Toxicology in Vitro.2019; 60: 252. CrossRef
The effect of desiccation on water sorption, solubility and hygroscopic volumetric expansion of dentine replacement materials
Ruba Mustafa, Ruwaida Z. Alshali, Nick Silikas
Dental Materials.2018; 34(8): e205. CrossRef
Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0℃/2 MPa Condition up to One Week: In Vivo MTT Method
Sun Mi Jang, Sin-Yeon Cho, Eui-Seong Kim, Il-Young Jung, Seung Jong Lee
Journal of Korean Dental Science.2016; 9(1): 1. CrossRef
Cytotoxic effects of one‐step self‐etching adhesives on an odontoblast cell line
Yoon Lee, So‐Youn An, Yoon‐Jung Park, Frank H. Yu, Joo‐Cheol Park, Deog‐Gyu Seo
Scanning.2016; 38(1): 36. CrossRef
Influence of Biodentine® - A Dentine Substitute - On Collagen Type I Synthesis in Pulp Fibroblasts In Vitro
Frangis Nikfarjam, Kim Beyer, Anke König, Matthias Hofmann, Manuel Butting, Eva Valesky, Stefan Kippenberger, Roland Kaufmann, Detlef Heidemann, August Bernd, Nadja Nicole Zöller, Dimitrios Karamichos
PLOS ONE.2016; 11(12): e0167633. CrossRef
Evaluation of cytotoxicity and gelatinases activity in 3T3 fibroblast cell by root repair materials
Varol Basak, Tuna Elif Bahar, Karsli Emine, Kasimoglu Yelda, Koruyucu Mine, Seymen Figen, Nurten Rustem
Biotechnology & Biotechnological Equipment.2016; 30(5): 984. CrossRef
In VitroCytotoxicity of Calcium Silicate-Based Endodontic Cement as Root-End Filling Materials
Selen Küçükkaya, Mehmet Ömer Görduysus, Naciye Dilara Zeybek, Sevda Fatma Müftüoğlu
Scientifica.2016; 2016: 1. CrossRef
Cytotoxic effects of mineral trioxide aggregate, calcium enrichedmixture cement, Biodentine and octacalcium pohosphate onhuman gingival fibroblasts
Eshagh A. Saberi, Narges Farhadmollashahi, Faroogh Ghotbi, Hamed Karkeabadi, Roholla Havaei
Journal of Dental Research, Dental Clinics, Dental Prospects.2016; 10(2): 75. CrossRef
Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials
Diana María Escobar-García, Eva Aguirre-López, Verónica Méndez-González, Amaury Pozos-Guillén
BioMed Research International.2016; 2016: 1. CrossRef
Cytotoxicity and osteogenic potential of silicate calcium cements as potential protective materials for pulpal revascularization
Eduardo A. Bortoluzzi, Li-na Niu, Chithra D. Palani, Ahmed R. El-Awady, Barry D. Hammond, Dan-dan Pei, Fu-cong Tian, Christopher W. Cutler, David H. Pashley, Franklin R. Tay
Dental Materials.2015; 31(12): 1510. CrossRef

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Temperature changes under demineralized dentin during polymerization of three resin-based restorative materials using QTH and LED units: Sayed-Mostafa Mousavinasab, Maryam Khoroushi, Mohammadreza Moharreri, Mohammad Atai; Restor Dent Endod 2014;39(3):155-163. Published online May 13, 2014; DOI: https://doi.org/10.5395/rde.2014.39.3.155

Abstract PDF PubReader ePub

Objectives

Light-curing of resin-based materials (RBMs) increases the pulp chamber temperature, with detrimental effects on the vital pulp. This in vitro study compared the temperature rise under demineralized human tooth dentin during light-curing and the degrees of conversion (DCs) of three different RBMs using quartz tungsten halogen (QTH) and light-emitting diode (LED) units (LCUs).

Materials and Methods

Demineralized and non-demineralized dentin disks were prepared from 120 extracted human mandibular molars. The temperature rise under the dentin disks (n = 12) during the light-curing of three RBMs, i.e. an Ormocer-based composite resin (Ceram. X, Dentsply DeTrey), a low-shrinkage silorane-based composite (Filtek P90, 3M ESPE), and a giomer (Beautifil II, Shofu GmbH), was measured with a K-type thermocouple wire. The DCs of the materials were investigated using Fourier transform infrared spectroscopy.

Results

The temperature rise under the demineralized dentin disks was higher than that under the non-demineralized dentin disks during the polymerization of all restorative materials (p < 0.05). Filtek P90 induced higher temperature rise during polymerization than Ceram.X and Beautifil II under demineralized dentin (p < 0.05). The temperature rise under demineralized dentin during Filtek P90 polymerization exceeded the threshold value (5.5℃), with no significant differences between the DCs of the test materials (p > 0.05).

Conclusions

Although there were no significant differences in the DCs, the temperature rise under demineralized dentin disks for the silorane-based composite was higher than that for dimethacrylate-based restorative materials, particularly with QTH LCU.

Citations

Citations to this article as recorded by

Effect of Shade and Light Curing Mode on the Degree of Conversion of Silorane-Based and Methacrylate-Based Resin Composites
Sayed-Mostafa Mousavinasab, Mohammad Atai, Negar Salehi, Arman Salehi
SSRN Electronic Journal.2024;[Epub] CrossRef
Pulp chamber temperature rise in light-cure bonding of brackets with and without primer, in intact versus restored teeth
Gabriela Cenci SCHMITZ, Fernanda de Souza HENKIN, Mauricio MEZOMO, Mariana MARQUEZAN, Gabriela BONACINA, Maximiliano Schünke GOMES, Eduardo Martinelli Santayana de LIMA
Dental Press Journal of Orthodontics.2023;[Epub] CrossRef
In Vivo Pulp Temperature Changes During Class V Cavity Preparation and Resin Composite Restoration in Premolars
DC Zarpellon, P Runnacles, C Maucoski, DJ Gross, U Coelho, FA Rueggeberg, CAG Arrais
Operative Dentistry.2021; 46(4): 374. CrossRef

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How to design in situ studies: an evaluation of experimental protocols: Young-Hye Sung, Hae-Young Kim, Ho-Hyun Son, Juhea Chang; Restor Dent Endod 2014;39(3):164-171. Published online May 13, 2014; DOI: https://doi.org/10.5395/rde.2014.39.3.164

Abstract PDF PubReader ePub

Objectives

Designing in situ models for caries research is a demanding procedure, as both clinical and laboratory parameters need to be incorporated in a single study. This study aimed to construct an informative guideline for planning in situ models relevant to preexisting caries studies.

Materials and Methods

An electronic literature search of the PubMed database was performed. A total 191 of full articles written in English were included and data were extracted from materials and methods. Multiple variables were analyzed in relation to the publication types, participant characteristics, specimen and appliance factors, and other conditions. Frequencies and percentages were displayed to summarize the data and the Pearson's chi-square test was used to assess a statistical significance (p < 0.05).

Results

There were many parameters commonly included in the majority of in situ models such as inclusion criteria, sample sizes, sample allocation methods, tooth types, intraoral appliance types, sterilization methods, study periods, outcome measures, experimental interventions, etc. Interrelationships existed between the main research topics and some parameters (outcome measures and sample allocation methods) among the evaluated articles.

Conclusions

It will be possible to establish standardized in situ protocols according to the research topics. Furthermore, data collaboration from comparable studies would be enhanced by homogeneous study designs.

Citations

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What is the effectiveness of titanium tetrafluoride to prevent or treat dental caries and tooth erosion? A systematic review
Ana Beatriz Chevitarese, Karla Lorene de França Leite, Guido A. Marañón-Vásquez, Danielle Masterson, Matheus Pithon, Lucianne Cople Maia
Acta Odontologica Scandinavica.2022; 80(6): 441. CrossRef
Effect of fluoride group on dental erosion associated or not with abrasion in human enamel: A systematic review with network metanalysis
Bruna Machado da Silva, Daniela Rios, Gerson Aparecido Foratori-Junior, Ana Carolina Magalhães, Marília Afonso Rabelo Buzalaf, Silvia De Carvalho Sales Peres, Heitor Marques Honório
Archives of Oral Biology.2022; 144: 105568. CrossRef
Multimodal Human and Environmental Sensing for Longitudinal Behavioral Studies in Naturalistic Settings: Framework for Sensor Selection, Deployment, and Management
Brandon M Booth, Karel Mundnich, Tiantian Feng, Amrutha Nadarajan, Tiago H Falk, Jennifer L Villatte, Emilio Ferrara, Shrikanth Narayanan
Journal of Medical Internet Research.2019; 21(8): e12832. CrossRef
Evaluation of an antibacterial orthodontic adhesive incorporated with niobium-based bioglass: an in situ study
Felipe Weidenbach DEGRAZIA, Aline Segatto Pires ALTMANN, Carolina Jung FERREIRA, Rodrigo Alex ARTHUR, Vicente Castelo Branco LEITUNE, Susana Maria Werner SAMUEL, Fabrício Mezzomo COLLARES
Brazilian Oral Research.2019;[Epub] CrossRef
A Review of the Common Models Used in Mechanistic Studies on Demineralization-Remineralization for Cariology Research
Ollie Yiru Yu, Irene Shuping Zhao, May Lei Mei, Edward Chin-Man Lo, Chun-Hung Chu
Dentistry Journal.2017; 5(2): 20. CrossRef
Effects of rinsing with arginine bicarbonate and urea solutions on initial enamel lesions in situ
Y Yu, X Wang, C Ge, B Wang, C Cheng, Y‐H Gan
Oral Diseases.2017; 23(3): 353. CrossRef
The cariogenicity of commercial infant formulas: a systematic review
S. F. Tan, H. J. Tong, X. Y. Lin, B. Mok, C. H. Hong
European Archives of Paediatric Dentistry.2016; 17(3): 145. CrossRef
In situ antibiofilm effect of glass-ionomer cement containing dimethylaminododecyl methacrylate
Jin Feng, Lei Cheng, Xuedong Zhou, Hockin H.K. Xu, Michael D. Weir, Markus Meyer, Hans Maurer, Qian Li, Matthias Hannig, Stefan Rupf
Dental Materials.2015; 31(8): 992. CrossRef

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Effect of 38% carbamide peroxide on the microleakage of silorane-based versus methacrylate-based composite restorations: Sedighe Sadat Hashemi Kamangar, Maryam Ghavam, Nazanin Mahinfar, Seyed Jalal Pourhashemi; Restor Dent Endod 2014;39(3):172-179. Published online May 13, 2014; DOI: https://doi.org/10.5395/rde.2014.39.3.172

Abstract PDF PubReader ePub: Objectives
This study aimed to assess the effect of 38% carbamide peroxide on the microleakage of class V cavities restored with either a silorane-based composite or two methacrylate-based composites.
Materials and Methods
A total of 96 class V cavities were prepared on the buccal surface of extracted human teeth with both enamel and dentin margins and were randomly assigned into three groups of Filtek P90 (3M-ESPE) + P90 system adhesive (3M-ESPE)(group A), Filtek Z250 (3M-ESPE) + Adper Prompt L-Pop (3M-ESPE)(group B) and Filtek Z350XT (3M-ESPE) + Adper Prompt L-Pop (group C). Half of the teeth were randomly underwent bleaching (38% carbamide peroxide, Day White, Discus Dental, applying for 15 min, twice a day for 14 day) while the remaining half (control) were not bleached. Dye penetration was measured following immersion in basic fuchsine. Data were statistically analyzed using Kruskal-Wallis and Mann-Whitney U tests at a level of 0.05.
Results
No significant differences were found between composites in the control groups in enamel (p = 0.171) or dentin (p = 0.094) margins. After bleaching, microleakage of Z250 (in enamel [p = 0.867] or dentin [p = 0.590] margins) and Z350 (in enamel [p = 0.445] or dentin [p = 0.591] margins) did not change significantly, but the microleakage of P90 significantly increased in both enamel (p = 0.042) and dentin (p = 0.002) margins.
Conclusions
No significant differences were noted between the bleached and control subgroups of two methacrylate-based composites in enamel or dentin margins. Microleakage of silorane-based composite significantly increased after bleaching.

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Enamel pretreatment with Er:YAG laser: effects on the microleakage of fissure sealant in fluorosed teeth: Mahtab Memarpour, Nasrin Kianimanesh, Bahareh Shayeghi; Restor Dent Endod 2014;39(3):180-186. Published online May 22, 2014; DOI: https://doi.org/10.5395/rde.2014.39.3.180

Abstract PDF PubReader ePub

Objectives

The purpose of this in vitro study was to evaluate the microleakage and penetration of fissure sealant in permanent molar teeth with fluorosis after pretreatment of the occlusal surface.

Materials and Methods

A total of 120 third molars with mild dental fluorosis were randomly divided into 6 groups (n = 20). The tooth surfaces were sealed with an unfilled resin fissure sealant (FS) material. The experimental groups included: 1) phosphoric acid etching (AE) + FS (control); 2) AE + One-Step Plus (OS, Bisco) + FS; 3) bur + AE + FS; 4) bur + AE + OS + FS; 5) Er:YAG laser + AE + FS; and 6) Er:YAG laser + AE + OS + FS. After thermocycling, the teeth were immersed in 0.5% fuchsin and sectioned. Proportions of mircoleakage (PM) and unfilled area (PUA) were measured by digital microscope.

Results

Overall, there were significant differences among all groups in the PM (p = 0.00). Group 3 showed the greatest PM, and was significantly different from groups 2 to 6 (p < 0.05). Group 6 showed the lowest PM. Pretreatment with Er:YAG with or without adhesive led to less PM than bur pretreatment. There were no significant differences among groups in PUA.

Conclusions

Conventional acid etching provided a similar degree of occlusal seal in teeth with fluorosis compared to those pretreated with a bur or Er:YAG laser. Pretreatment of pits and fissures with Er:YAG in teeth with fluorosis may be an alternative method before fissure sealant application.

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Comparative Evaluation of Penetrative and Adaptive Properties of Unfilled and Filled Resin-Based Sealants When Placed using Conventional acid Etching, Lasing, and Fissurotomy Bur Technique of Enamel Preparation
Poonam Ramrao Shingare, Vishwas Chaugule, Neha Pankey, Pallavi Kakade
Contemporary Clinical Dentistry.2022; 13(4): 349. CrossRef
Laser Tooth Preparation for Pit and Fissure Sealing
Yair Schwimmer, Nurit Beyth, Diana Ram, Eitan Mijiritsky, Esti Davidovich
International Journal of Environmental Research and Public Health.2020; 17(21): 7813. CrossRef
The clinical effects of laser preparation of tooth surfaces for fissure sealants placement: a systematic review and meta-analysis
Yunhan Zhang, Yan Wang, Yandi Chen, Yang Chen, Qiong Zhang, Jing Zou
BMC Oral Health.2019;[Epub] CrossRef
Effect of laser preparation on adhesion of a self‐adhesive flowable composite resin to primary teeth
Mahtab Memarpour, Fereshteh Shafiei, Faranak Razmjoei, Nasrin Kianimanesh
Microscopy Research and Technique.2016; 79(4): 334. CrossRef

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The effects of bone morphogenetic protein-2 and enamel matrix derivative on the bioactivity of mineral trioxide aggregate in MC3T3-E1cells: Youngdan Jeong, Wonkyung Yang, Hyunjung Ko, Miri Kim; Restor Dent Endod 2014;39(3):187-194. Published online June 19, 2014; DOI: https://doi.org/10.5395/rde.2014.39.3.187

Abstract PDF PubReader ePub

Objectives

The effects of bone morphogenetic protein-2 (BMP-2) and enamel matrix derivative (EMD) respectively with mineral trioxide aggregate (MTA) on hard tissue regeneration have been investigated in previous studies. This study aimed to compare the osteogenic effects of MTA/BMP-2 and MTA/EMD treatment in MC3T3-E1 cells.

Materials and Methods

MC3T3-E1 cells were treated with MTA (ProRoot, Dentsply), BMP-2 (R&D Systems), EMD (Emdogain, Straumann) separately and MTA/BMP-2 or MTA/EMD combination. Mineralization was evaluated by staining the calcium deposits with alkaline phosphatase (ALP, Sigma-Aldrich) and Alizarin red (Sigma-Aldrich). The effects on the osteoblast differentiation were evaluated by the expressions of osteogenic markers, including ALP, bone sialoprotein (BSP), osteocalcin (OCN), osteopontin (OPN) and osteonectin (OSN), as determined by reverse-transcription polymerase chain reaction analysis (RT-PCR, AccuPower PCR, Bioneer).

Results

Mineralization increased in the BMP-2 and MTA/BMP-2 groups and increased to a lesser extent in the MTA/EMD group but appeared to decrease in the MTA-only group based on Alizarin red staining. ALP expression largely decreased in the EMD and MTA/EMD groups based on ALP staining. In the MTA/BMP-2 group, mRNA expression of OPN on day 3 and BSP and OCN on day 7 significantly increased. In the MTA/EMD group, OSN and OCN gene expression significantly increased on day 7, whereas ALP expression decreased on days 3 and 7 (p < 0.05).

Conclusions

These results suggest the MTA/BMP-2 combination promoted more rapid differentiation in MC3T3-E1 cells than did MTA/EMD during the early mineralization period.

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Elucidating epigenetic mechanisms governing odontogenic differentiation in dental pulp stem cells: an in-depth exploration
Lei Huang, Xuan Chen, Xiaoxia Yang, Yinchun Zhang, Yiyun Liang, Xiaoling Qiu
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Evaluation of the genotoxicity, cytotoxicity, and bioactivity of calcium silicate-based cements
Merve Esen, Yeliz Guven, Mehmet Fatih Seyhan, Handan Ersev, Elif Bahar Tuna-Ince
BMC Oral Health.2024;[Epub] CrossRef
GelMA‐based hydrogel biomaterial scaffold: A versatile platform for regenerative endodontics
Lei Huang, Xuan Chen, XiaoXia Yang, Yinchun Zhang, Xiaoling Qiu
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Md. Niaj Morshed, Reshmi Akter, Imran Mahmud, Ah-Yeong Gwon, Jin Woo Jeang, Yeong-Geun Lee, Dae Won Park, Deok Chun Yang, Yeon Ju Kim, Se-Chan Kang
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Da Eun Lee, Kun Hee Park, Joo-Hyun Hong, Seon Hee Kim, Ki-Moon Park, Ki Hyun Kim
Archives of Pharmacal Research.2023; 46(9-10): 771. CrossRef
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Reshmi Akter, Vinothini Boopathi, Muhammad Awais, Juha Park, Byoung Man Kong, Se-Woung Oh, Ji-Hyung Oh, Jong Chan Ahn, Deok Chun Yang
ACS Food Science & Technology.2023; 3(11): 1975. CrossRef
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Chang‐Hwan Seong, Norika Chiba, Mardiyantoro Fredy, Joji Kusuyama, Kiyohide Ishihata, Toshiro Kibe, Muhammad Subhan Amir, Ryohei Tada, Tomokazu Ohnishi, Norifumi Nakamura, Tetsuya Matsuguchi
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Mijoo Kim, Marc Hayashi, Bo Yu, Thomas K. Lee, Reuben H. Kim, Deuk-Won Jo
Polymers.2022; 14(12): 2315. CrossRef
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Xuming Yang, Donggang Mou, Qunying Yu, Jimei Zhang, Ying Xiong, Zhimin Zhang, Shan Xing
Annals of Anatomy - Anatomischer Anzeiger.2022; 239: 151819. CrossRef
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Ebrahim Patel, Priyamvada Pradeep, Pradeep Kumar, Yahya E. Choonara, Viness Pillay
Journal of Biomedical Materials Research Part B: Applied Biomaterials.2020; 108(1): 201. CrossRef
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Nina Bono, Paolo Tarsini, Gabriele Candiani
Journal of Applied Biomaterials & Functional Materials.2019;[Epub] CrossRef
An assessment of the overexpression of BMP‐2 in transfected human osteoblast cells stimulated by mineral trioxide aggregate and Biodentine
E. M. Rodrigues, A. L. Gomes‐Cornélio, A. Soares‐Costa, L. P. Salles, M. Velayutham, C. Rossa‐Junior, J. M. Guerreiro‐Tanomaru, M. Tanomaru‐Filho
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Hyung-Mun Yun, Seok-Woo Chang, Kyung-Ran Park, Lan Herr, Eun-Cheol Kim
Journal of Endodontics.2016; 42(2): 269. CrossRef
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Seok-Woo Chang, Ji-Youn Kim, Mi-Joo Kim, Ga-Hyun Kim, Jin-Kyu Yi, Deok-Won Lee, Kee-Yeon Kum, Eun-Cheol Kim
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Toyonobu Maeda, Atsuko Suzuki, Satoshi Yuzawa, Yuh Baba, Yuichi Kimura, Yasumasa Kato
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Comparative efficacy of photo-activated disinfection and calcium hydroxide for disinfection of remaining carious dentin in deep cavities: a clinical study: Sidhartha Sharma, Ajay Logani, Naseem Shah; Restor Dent Endod 2014;39(3):195-200. Published online June 26, 2014; DOI: https://doi.org/10.5395/rde.2014.39.3.195

Abstract PDF PubReader ePub

Objectives

To comparatively evaluate the efficacy of photo-activated disinfection (PAD), calcium hydroxide (CH) and their combination on the treatment outcome of indirect pulp treatment (IPT).

Materials and Methods

Institutional ethical clearance and informed consent of the patients were taken. The study was also registered with clinical registry of India. Sixty permanent molars exhibiting deep occlusal carious lesion in patients with the age range of 18 - 22 yr were included. Clinical and radiographic evaluation and set inclusion and exclusion criteria's were followed. Gross caries excavation was accomplished. In group I (n = 20) PAD was applied for sixty seconds. In group II (n = 20), CH was applied to the remaining carious dentin, while in group III (n = 20), PAD application was followed by CH placement. The teeth were permanently restored. They were clinically and radiographically followed-up at 45 day, 6 mon and 12 mon. Relative density of the remaining affected dentin was measured by 'Radiovisiography (RVG) densitometric' analysis.

Results

Successful outcome with an increase in radiographic grey values were observed in all three groups. However, on inter-group comparison, this change was not significant (p > 0.05).

Conclusions

PAD and CH both have equal disinfection efficacy in the treatment of deep carious dentin. PAD alone is as effective for treatment of deep carious lesion as calcium hydroxide and hence can be used as an alternative to CH. They can be used independently in IPT, since combining both does not offer any additional therapeutic benefits.

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Clinical and Radiographic Evaluation between Conventional Mineral Trioxide Aggregate and Gel-based Mineral Trioxide Aggregate in Indirect Pulp Therapy: A Randomized Clinical Trial
Yusuf Chunawala, BK Vanishree, Supriya S Dighe, Rooposhi Saha
International Journal of Clinical Pediatric Dentistry.2025; 17(12): 1383. CrossRef
Potentialities of photoactivated disinfection in dentistry
E.I. Utkina, M.A. Gorbatova, A.M. Grjibovski, L.N. Gorbatova, A.A. Simakova
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Clinical and radiographic evaluation of diode laser and chemical disinfection in comparison to selective caries removal in management of patients with deep carious lesions
Mohamed Bahgat AbdelHamid, Ahmed Fawzy Abo Elezz, Ola M. Ibrahim Fahmy
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Comparative analysis of physicochemical properties of root perforation sealer materials: Maura Cristiane Gonçales Orçati Dorileo, Fábio Luis Miranda Pedro, Matheus Coelho Bandeca, Orlando Aguirre Guedes, Ricardo Dalla Villa, Alvaro Henrique Borges; Restor Dent Endod 2014;39(3):201-209. Published online June 30, 2014; DOI: https://doi.org/10.5395/rde.2014.39.3.201

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Objectives

This study evaluated the solubility, dimensional alteration, pH, electrical conductivity, and radiopacity of root perforation sealer materials.

Materials and Methods

For the pH test, the samples were immersed in distilled water for different periods of time. Then, the samples were retained in plastic recipients, and the electrical conductivity of the solution was measured. The solubility, dimensional alteration, and radiopacity properties were evaluated according to Specification No. 57 of the American National Standards Institute/American Dental Association (ANSI/ADA). Statistical analyses were carried out using analysis of variance (ANOVA) and Tukey's test at a significance level of 5%. When the sample distribution was not normal, a nonparametric ANOVA was performed with a Kruskal-Wallis test (α = 0.05).

Results

The results showed that white structural Portland cement (PC) had the highest solubility, while mineral trioxide aggregate (MTA)-based cements, ProRoot MTA (Dentsply-Tulsa Dental) and MTA BIO (Ângelus Ind. Prod.), had the lowest values. MTA BIO showed the lowest dimensional alteration values and white PC presented the highest values. No differences among the tested materials were observed in the the pH and electrical conductivity analyses. Only the MTA-based cements met the ANSI/ADA recommendations regarding radiopacity, overcoming the three steps of the aluminum step wedge.

Conclusions

On the basis of these results, we concluded that the values of solubility and dimensional alteration of the materials were in accordance with the ANSI/ADA specifications. PCs did not fulfill the ANSI/ADA requirements regarding radiopacity. No differences were observed among the materials with respect to the pH and electrical conductivity analyses.

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Chemical and in vivo analyses of calcium silicate‐based materials in bone and connective tissues
Ana Cristina Padilha Janini, Lauter Eston Pelepenko, Brenda Fornazaro Moraes, Victor Augusto Benedicto dos Santos, Matheus Barros‐Costa, Isabela Alvarenga Maciel dos Santos, Fábio Roberto de Souza Batista, Juliana de Aguiar Silveira Meira, Mariza Akemi Ma
International Endodontic Journal.2025; 58(3): 484. CrossRef
Physicochemical and antibacterial properties of ZnO/chitosan-modified mineral trioxide aggregate composites
Mariyam Mariyam, Siti Sunarintyas, Leny Yuliatun, Dyah Irnawati, Adhi Dwi Hatmanto, Nuryono Nuryono
Case Studies in Chemical and Environmental Engineering.2024; 9: 100749. CrossRef
Portland Cement: An Overview as a Root Repair Material
Shahriar Shahi, Elaheh Fakhri, Hamidreza Yavari, Solmaz Maleki Dizaj, Sara Salatin, Khadijeh Khezri, Victor Feitosa
BioMed Research International.2022;[Epub] CrossRef
A micro-computed tomographic study using a novel test model to assess the filling ability and volumetric changes of bioceramic root repair materials
Fernanda Ferrari Esteves Torres, Jader Camilo Pinto, Gabriella Oliveira Figueira, Juliane Maria Guerreiro-Tanomaru, Mario Tanomaru-Filho
Restorative Dentistry & Endodontics.2021;[Epub] CrossRef
COMPARISON OF MINERAL TRIOXIDE AGGREGATE, ENDOSEQUENCE ROOT REPAIR MATERIAL, AND BIODENTINE USED FOR REPAIRING ROOT PERFORATIONS: A SYSTEMATIC REVIEW
Faisal ALGHAMDİ, Esraa ALJAHDALİ
Cumhuriyet Dental Journal.2019; 22(4): 469. CrossRef
Effects of the addition of nanoparticulate calcium carbonate on setting time, dimensional change, compressive strength, solubility and pH of MTA
A. Bernardi, E. A. Bortoluzzi, W. T. Felippe, M. C. S. Felippe, W. S. Wan, C. S. Teixeira
International Endodontic Journal.2017; 50(1): 97. CrossRef
The use of a biocompatible cement in endodontic surgery. A randomized clinical trial 1
Sérgio Ribeiro da Silva, José Dias da Silva Neto, Taylor Brandão Schnaider, Daniela Francescato Veiga, Neil Ferreira Novo, Marcos Mesquita Filho, Lydia Masako Ferreira
Acta Cirurgica Brasileira.2016; 31(6): 422. CrossRef
Evaluation of Sealing Effect and Working Time of Root Canal Filling MTA Materials
Hyojin Kim, Youngjin Kim, Soonhyeun Nam, Kwon Taeyub, Hyunjung Kim
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY.2016; 43(2): 129. CrossRef
Portland cement versus MTA as a root-end filling material. A pilot study
Sérgio Ribeiro da Silva, José Dias da Silva Neto, Daniela Francescato Veiga, Taylor Brandão Schnaider, Lydia Masako Ferreira
Acta Cirurgica Brasileira.2015; 30(2): 160. CrossRef

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Prevalence of referral reasons and clinical symptoms for endodontic referrals: Seonah Kim; Restor Dent Endod 2014;39(3):210-214. Published online June 23, 2014; DOI: https://doi.org/10.5395/rde.2014.39.3.210

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Objectives

To investigate the prevalence of different primary reasons for endodontic referrals and the clinical symptoms of the referred cases.

Materials and Methods

Clinical data of total endodontic treatment cases (1,014 teeth) including endodontic referral cases (224 teeth) between January 1, 2010 and December 31, 2012, at Kangdong Sacred Heart Hospital, were investigated retrospectively. The one major reason for referral, the clinical symptoms, and the resulting treatment procedures of referral cases were recorded. The percentages of clinical symptoms of the endodontic referral cases and the total endodontic treatment cases were compared by χ² test for each symptom.

Results

Persistent pain was the most frequent reason for endodontic referral (29.5%), followed by presence of gingival swelling and sinus tract (24.1%), and apical radiolucency (12.9%). Referrals in cases involving endodontic difficulties such as canal calcification, broken instruments, post, perforation, and resorption were less than 5.0%, respectively. The percentages of four major clinical symptoms of pain, apical radiolucency, previous endodontic treatment, and gingival swelling and sinus tract were significantly higher in the endodontic referral cases than those in the total endodontic cases (p = 0.001). Among the included referral cases, 72.8% were treated with nonsurgical endodontic treatment only. Teeth other than the referred teeth were diagnosed as the origin of the problem in 5.8% of the referrals.

Conclusions

The high prevalence of pain, apical radiolucency, previous treatment, and gingival swelling and sinus tract in endodontic referral cases suggest that these symptoms may be what general practitioners consider to be difficult and refer to endodontists.

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VARIABLES AFFECTING PATIENT REFERRALS FROM GENERAL DENTAL PRACTITIONERS TO ENDODONTISTS
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Su-Jin Jeon, Soo-Jeong Hwang, Min-Seock Seo
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D. Sebring, H. Dimenäs, S. Engstrand, T. Kvist
International Endodontic Journal.2017; 50(7): 629. CrossRef

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Case Reports

Mental nerve paresthesia secondary to initiation of endodontic therapy: a case report: Syed Mukhtar-Un-Nisar Andrabi, Sharique Alam, Afaf Zia, Masood Hasan Khan, Ashok Kumar; Restor Dent Endod 2014;39(3):215-219. Published online May 8, 2014; DOI: https://doi.org/10.5395/rde.2014.39.3.215

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Whenever endodontic therapy is performed on mandibular posterior teeth, damage to the inferior alveolar nerve or any of its branches is possible. Acute periapical infection in mandibular posterior teeth may also sometimes disturb the normal functioning of the inferior alveolar nerve. The most common clinical manifestation of these insults is the paresthesia of the inferior alveolar nerve or mental nerve paresthesia. Paresthesia usually manifests as burning, prickling, tingling, numbness, itching or any deviation from normal sensation. Altered sensation and pain in the involved areas may interfere with speaking, eating, drinking, shaving, tooth brushing and other events of social interaction which will have a disturbing impact on the patient. Paresthesia can be short term, long term or even permanent. The duration of the paresthesia depends upon the extent of the nerve damage or persistence of the etiology. Permanent paresthesia is the result of nerve trunk laceration or actual total nerve damage. Paresthesia must be treated as soon as diagnosed to have better treatment outcomes. The present paper describes a case of mental nerve paresthesia arising after the start of the endodontic therapy in left mandibular first molar which was managed successfully by conservative treatment.

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Neeta Patel, Akshayraj Langaliya, Shikha Kanodia, Aravind Kumbhar, Aastha Buch, Aarshvi Shah, Himani Bhatt, Drashti Panchal, Sharan Shah, Jinali Shah, Darko Macan
Case Reports in Dentistry.2021; 2021: 1. CrossRef
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Michał Puciło, Mariusz Lipski, Magdalena Sroczyk-Jaszczyńska, Aleksandra Puciło, Alicja Nowicka
Surgical and Radiologic Anatomy.2020; 42(5): 529. CrossRef
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R. Castro, M. Guivarc'h, J.M. Foletti, J.H. Catherine, C. Chossegros, L. Guyot
Journal of Stomatology, Oral and Maxillofacial Surgery.2018; 119(5): 412. CrossRef
Neuropathy of Trigeminal Nerve Branches After Oral and Maxillofacial Treatment
Jimoh Olubanwo Agbaje, Elke Van de Casteele, Marjolein Hiel, Ciska Verbaanderd, Ivo Lambrichts, Constantinus Politis
Journal of Maxillofacial and Oral Surgery.2016; 15(3): 321. CrossRef
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Umut Demetoglu, Gokhan Ozkan, Hasan Onur Simsek
Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology.2016; 28(3): 267. CrossRef

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Esthetic rehabilitation of single anterior edentulous space using fiber-reinforced composite: Hyeon Kim, Min-Ju Song, Su-Jung Shin, Yoon Lee, Jeong-Won Park; Restor Dent Endod 2014;39(3):220-225. Published online May 19, 2014; DOI: https://doi.org/10.5395/rde.2014.39.3.220

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A fiber-reinforced composite (FRC) fixed prosthesis is an innovative alternative to a traditional metal restoration, as it is a conservative treatment method. This case report demonstrates a detailed procedure for restoring a missing anterior tooth with an FRC. A 44-year-old woman visited our department with an avulsed tooth that had fallen out on the previous day and was completely dry. This tooth was replanted, but it failed after one year. A semi-direct technique was used to fabricate a FRC fixed partial prosthesis for its replacement. The FRC framework and the pontic were fabricated using a duplicated cast model and nanofilled composite resin. Later on, interproximal contact, tooth shape, and shade were adjusted at chairside. This technique not only enables the clinician to replace a missing tooth immediately after extraction for minimizing esthetic problems, but it also decreases both tooth reduction and cost.

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Anterior provisional fixed partial dentures: A finite element analysis
Nouf Almeganni, Rotana Abulaban, Ghada Naguib, Mohamed Tharwat, Hani M. Nassar
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Gözde YALÇIN, Asude Dilek NALBANT
Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi.2022; : 1. CrossRef
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A New Technique for Direct Fabrication of Fiber-Reinforced Composite Bridge: A Long-Term Clinical Observation
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Ana Todorović, Danica Popović, Igor Djordjević, Vojkan Lazić
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Sun Mi Jang, Sin-Yeon Cho, Eui-Seong Kim, Il-Young Jung, Seung Jong Lee
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Mateus Rodrigues Tonetto, Milton Carlos Kuga, Fausto Frizzera, Matheus Coelho Bandeca, Shilpa H Bhandi, Célia Regina Maio Pinzan-Vercelino, Monica Barros da Silva, Kamila Figueiredo Pereira
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An esthetic appliance for the management of crown-root fracture: a case report: Sang-Min Jeon, Kang-Hee Lee, Bock-Young Jung; Restor Dent Endod 2014;39(3):226-229. Published online May 22, 2014; DOI: https://doi.org/10.5395/rde.2014.39.3.226

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Orthodontic extrusion is usually performed by means of a fixed orthodontic appliance that utilizes arch wire attached to adjacent teeth and transfers the desired force by elastic from the wire to the root. However, clinicians often encounter cases where the bonding required for tooth traction is not possible because the adjacent teeth have been restored with ceramic or veneer. The purpose of this case report is to describe a modified orthodontic extrusion appliance that is useful when conventional orthodontic treatment is not possible. The modified appliance was fabricated using an artificial tooth, clear plastic sheeting, and a braided fiber-reinforced composite strip that covered adjacent teeth without bonding. It satisfied the esthetic and functional needs of the patient and established the optimal biologic width.

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Esthetic enhancement of a traumatized anterior tooth with a combination of forced eruption and tooth alignment: a case report
So-Hee Kang, Jung-Hong Ha, Myoung-Uk Jin, Sung-Kyo Kim, Young-Kyung Kim
Restorative Dentistry & Endodontics.2016; 41(3): 210. CrossRef

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The use of platelet rich plasma in the treatment of immature tooth with periapical lesion: a case report: Günseli Güven Polat, Ceren Yıldırım, Özlem Martı Akgün, Ceyhan Altun, Didem Dinçer, Cansel Köse Özkan; Restor Dent Endod 2014;39(3):230-234. Published online June 2, 2014; DOI: https://doi.org/10.5395/rde.2014.39.3.230

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This study describes the treatment of an immature permanent tooth with periapical lesion which was treated with regenerative approach using platelet rich plasma (PRP). The root canal of immature human permanent tooth with periapical lesion was gently debrided of necrotic tissue and disinfected with 2.5% NaOCl, and then medicated with triple antibiotic paste comprised of ciprofloxacin, metronidazole, and tetracycline. When the tooth was asymptomatic, PRP and mineral trioxide aggregate (MTA) were placed into the root canal. Six months after PRP treatment, radiographical examination revealed resolution of the radiolucency and progressive thickening of the root wall and apical closure. Our findings suggest that PRP can be used for the treatment of immature permanent teeth with periapical lesion, as part of a regenerative endodontic treatment procedure.

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Open Lecture on Statistics

Statistical notes for clinical researchers: Nonparametric statistical methods: 1. Nonparametric methods for comparing two groups: Hae-Young Kim; Restor Dent Endod 2014;39(3):235-239. Published online May 20, 2014; DOI: https://doi.org/10.5395/rde.2014.39.3.235

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Rotational stability of endodontic motors: Se-Hee Park; Restor Dent Endod 2014;39(3):240-240. Published online May 20, 2014; DOI: https://doi.org/10.5395/rde.2014.39.3.240

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