Restorative Dentistry & Endodontics

Miri Kim

6 Articles

Effects of the cathepsin K inhibitor with mineral trioxide aggregate cements on osteoclastic activity: Hee-Sun Kim, Soojung Kim, Hyunjung Ko, Minju Song, Miri Kim; Restor Dent Endod 2019;44(2):e17. Published online April 23, 2019; DOI: https://doi.org/10.5395/rde.2019.44.e17

Objectives

Root resorption is an unexpected complication after replantation procedures. Combining anti-osteoclastic medicaments with retrograde root filling materials may avert this resorptive activity. The purpose of this study was to assess effects of a cathepsin K inhibitor with calcium silicate-based cements on osteoclastic activity.

Methods

MC3T3-E1 cells were cultured for biocompatibility analyses. RAW 264.7 cells were cultured in the presence of the receptor activator of nuclear factor-kappa B and lipopolysaccharide, followed by treatment with Biodentine (BIOD) or ProRoot MTA with or without medicaments (Odanacatib [ODN], a cathepsin inhibitor and alendronate, a bisphosphonate). After drug treatment, the cell counting kit-8 assay and Alizarin red staining were performed to evaluate biocompatibility in MC3T3-E1 cells. Reverse-transcription polymerase chain reaction, tartrate-resistant acid phosphatase (TRAP) staining and enzyme-linked immunosorbent assays were performed in RAW 264.7 cells to determine the expression levels of inflammatory cytokines, interleukin (IL)-1β, IL-6, tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2). Data were analyzed by one-way analysis of variance and Tukey's post hoc test (p < 0.05).

Results

Biocompatibility results showed that there were no significant differences among any of the groups. RAW 264.7 cells treated with BIOD and ODN showed the lowest levels of TNF-α and PGE2. Treatments with BIOD + ODN were more potent suppressors of inflammatory cytokine expression (p < 0.05).

Conclusion

The cathepsin K inhibitor with calcium silicate-based cement inhibits osteoclastic activity. This may have clinical application in preventing inflammatory root resorption in replanted teeth.

Citations

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Root-filling materials for endodontic surgery: biological and clinical aspects
Andreas Koutroulis, Vasileios Kapralos, Dag Ørstavik, Pia Titterud Sunde
Biomaterial Investigations in Dentistry.2024; 11: 115. CrossRef
Effect of intra‐alveolar delivery of Frondoside A on inflammatory response of delayed tooth replantation
Lar Herr, Ju Ri Ye, Sang Wook Kang, Sang Tae Ro, Yong Kwon Chae, Ko Eun Lee, Mi Sun Kim, Myeong Kwan Jih, Chunui Lee, Sung Chul Choi, Ok Hyung Nam
Dental Traumatology.2024; 40(2): 178. CrossRef
Bone-targeting PLGA derived lipid drug delivery system ameliorates bone loss in osteoporotic ovariectomized rats
Youyun Zeng, Yiding Shen, Shuyi Wu, Lei Cai, Zhen Wang, Kexin Cai, Jiating Shen, Kendrick Hii Ru Yie, Hualin Zhang, Lihua Xu, Jinsong Liu
Materials & Design.2022; 221: 110967. CrossRef

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Cytotoxicity and biocompatibility of Zirconia (Y-TZP) posts with various dental cements: Hyeongsoon Shin, Hyunjung Ko, Miri Kim; Restor Dent Endod 2016;41(3):167-175. Published online May 30, 2016; DOI: https://doi.org/10.5395/rde.2016.41.3.167

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Objectives

Endodontically treated teeth with insufficient tooth structure are often restored with esthetic restorations. This study evaluated the cytotoxicity and biological effects of yttria partially stabilized zirconia (Y-TZP) blocks in combination with several dental cements.

Materials and Methods

Pairs of zirconia cylinders with medium alone or cemented with three types of dental cement including RelyX U200 (3M ESPE), FujiCEM 2 (GC), and Panavia F 2.0 (Kuraray) were incubated in medium for 14 days. The cytotoxicity of each supernatant was determined using 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays on L929 fibroblasts and MC3T3-E1 osteoblasts. The levels of interleukin-6 (IL-6) mRNA were evaluated by reverse transcription polymerase chain reaction (RT-PCR), and IL-6 protein was evaluated by enzyme-linked immunosorbent assays (ELISA). The data were analyzed using one-way ANOVA and Tukey post-hoc tests. A p < 0.05 was considered statistically significant.

Results

The MTT assays showed that MC3T3-E1 osteoblasts were more susceptible to dental cements than L929 fibroblasts. The resin based dental cements increased IL-6 expression in L929 cells, but reduced IL-6 expression in MC3T3-E1 cells.

Conclusions

Zirconia alone or blocks cemented with dental cement showed acceptable biocompatibilities. The results showed resin-modified glass-ionomer based cement less produced inflammatory cytokines than other self-adhesive resin-based cements. Furthermore, osteoblasts were more susceptible than fibroblasts to the biological effects of dental cement.

Citations

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Digital light processing 3D printing of yttria stabilized zirconia ceramics: microstructures, characterizations, and cytocompatibility
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3D printing of ceramics: Advantages, challenges, applications, and perspectives
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Pune Nina Paqué, Mutlu Özcan
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Afida Jemat, Masfueh Razali, Yuichi Otsuka, Mariyam Jameelah Ghazali
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Tianyu Yu, Xiaolong Zhu, Hongwei Yu, Pan Wu, Chun Li, Xiaoxiao Han, Mingjun Chen
Journal of Manufacturing Processes.2023; 94: 120. CrossRef
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Mohammad Asaduzzaman Chowdhury, Nayem Hossain, Md. Golam Mostofa, Md. Riyad Mia, Md. Tushar, Md. Masud Rana, Md. Helal Hossain
Heliyon.2023; 9(1): e12711. CrossRef
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Elia Marin, Giada Bassi, Orion Yoshikawa, Francesco Boschetto, Wenliang Zhu, Arianna Rossi, Alex Lanzutti, Huaizhong Xu, Monica Montesi, Silvia Panseri, Giuseppe Pezzotti
Journal of Materials Science.2023; 58(27): 11218. CrossRef
Nano-β-tricalcium phosphate incorporated root dentin adhesive in the bonding interface of yttria-stabilized tetragonal zirconia polycrystalline post
Amal S. Al-Qahtani, Huda I. Tulbah, Mashael Binhasan, Mai M. Alhamdan, Imran Farooq, Fahim Vohra, Tariq Abduljabbar
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Shunv Ying, Song Chen, Siyuan Wang, Lingli Xu, Xiaofeng Wang, Fuming He, Wei Liu
BMC Oral Health.2022;[Epub] CrossRef
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Comparison of Regular and Speed Sintering on Low-Temperature Degradation and Fatigue Resistance of Translucent Zirconia Crowns for Implants: An In Vitro Study
Suchada Kongkiatkamon, Chaimongkon Peampring
Journal of Functional Biomaterials.2022; 13(4): 281. CrossRef
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Hui Sun, Kefeng Gao, Zhe Yi, Chengwei Han, Zengqian Liu, Qiang Wang, Qing Zhou, Zhefeng Zhang
Frontiers in Bioengineering and Biotechnology.2022;[Epub] CrossRef
Characterisation of Selected Materials in Medical Applications
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Polymers.2022; 14(8): 1526. CrossRef
Adhesive bond integrity of Y‐TZP post with calcium fluoride infiltrated resin dentin adhesive: An SEM, EDX, FTIR and micro‐Raman study
Eman M. AlHamdan, Samar Al‐Saleh, Mohammad H. AlRefeai, Imran Farooq, Eisha Abrar, Fahim Vohra, Tariq Abduljabbar
Surface and Interface Analysis.2021; 53(11): 956. CrossRef
Additive Manufacturing (3D PRINTING) Methods and Applications in Dentistry
Elif DEMİRALP, Gülsüm DOĞRU, Handan YILMAZ
Clinical and Experimental Health Sciences.2021; 11(1): 182. CrossRef
Interleukin-1β activity in gingival crevicular fluid of abutment teeth with temporary fixed restorations versus final fixed restorations: Prospective observational study
Amal Abdallah A. Abo-Elmagd, Dina Sabry, Ebtehal Mohammed
The Saudi Dental Journal.2021; 33(6): 322. CrossRef
Change in the Microhardness of Composite Ceramics at the CaO–ZrO2/CaO–ZrO2 + Al2O3 Interface
A. A. Dmitrievskiy, D. G. Zhigacheva, G. V. Grigoriev, P. N. Ovchinnikov
Journal of Surface Investigation: X-ray, Synchrotron and Neutron Techniques.2021; 15(S1): S137. CrossRef
Cytotoxicity of two self-adhesive flowable composites on bovine dental pulp-derived cells
Firdevs KAHVECİOĞLU, Türkay KÖLÜŞ, Fatma SAĞ GÜNGÖR, Hayriye Esra ÜLKER
Journal of Health Sciences and Medicine.2021; 4(2): 209. CrossRef
Cytotoxicity and biocompatibility of high mol% yttria containing zirconia
Gulsan Ara Sathi Kazi, Ryo Yamagiwa
Restorative Dentistry & Endodontics.2020;[Epub] CrossRef
Cytotoxic effects of different self-adhesive resin cements: Cell viability and induction of apoptosis
Soner Şişmanoğlu, Mustafa Demirci, Helmut Schweikl, Gunes Ozen-Eroglu, Esin Cetin-Aktas, Serap Kuruca, Safa Tuncer, Neslihan Tekce
The Journal of Advanced Prosthodontics.2020; 12(2): 89. CrossRef
Effects of nano-zirconia fillers conditioned with phosphate ester monomers on the conversion and mechanical properties of Bis-GMA- and UDMA-based resin composites
Jiaxue Yang, Jiadi Shen, Xinyi Wu, Feng He, Haifeng Xie, Chen Chen
Journal of Dentistry.2020; 94: 103306. CrossRef
Effect of APTES- or MPTS-Conditioned Nanozirconia Fillers on Mechanical Properties of Bis-GMA-Based Resin Composites
Jiaxue Yang, Mengyuan Liao, Gaoying Hong, Shiqi Dai, Jiadi Shen, Haifeng Xie, Chen Chen
ACS Omega.2020; 5(50): 32540. CrossRef
In Vitro Cytotoxicity of Self-Adhesive Dual-Cured Resin Cement Polymerized Beneath Three Different Cusp Inclinations of Zirconia
Chang-Yuan Zhang, Yi-Ling Cheng, Xin-Wen Tong, Hao Yu, Hui Cheng
BioMed Research International.2019; 2019: 1. CrossRef
Investigations on the corrosion behaviour and biocompatibility of magnesium alloy surface composites AZ91D-ZrO2 fabricated by friction stir processing
R. Vaira Vignesh, R. Padmanaban, M. Govindaraju, G. Suganya Priyadharshini
Transactions of the IMF.2019; 97(5): 261. CrossRef
Microwave assisted synthesis and antimicrobial activity of Fe3O4-doped ZrO2 nanoparticles
M. Imran, Saira Riaz, Ifra Sanaullah, Usman Khan, Anjum N. Sabri, Shahzad Naseem
Ceramics International.2019; 45(8): 10106. CrossRef
Additive manufacturing of ceramics for dental applications: A review
Raquel Galante, Celio G. Figueiredo-Pina, Ana Paula Serro
Dental Materials.2019; 35(6): 825. CrossRef
Reinforcement of dental resin composite via zirconium hydroxide coating and phosphate ester monomer conditioning of nano-zirconia fillers
Xinyi Wu, Shiqi Dai, Ying Chen, Feng He, Haifeng Xie, Chen Chen
Journal of the Mechanical Behavior of Biomedical Materials.2019; 94: 32. CrossRef
Effects of TiO2 on microstructural, mechanical properties and in-vitro bioactivity of plasma sprayed yttria stabilised zirconia coatings for dental application
A. Jemat, M.J. Ghazali, M. Razali, Y. Otsuka, A. Rajabi
Ceramics International.2018; 44(4): 4271. CrossRef
In vitro evaluation of cytotoxicity of different self-adhesive resin cements
Necla Demir, Firdevs Kahvecioğlu, Muhammet Karcı, Hayriye Esra Ülker, Nuray Günaydın
Acta Odontologica Turcica.2017;[Epub] CrossRef

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The effects of bone morphogenetic protein-2 and enamel matrix derivative on the bioactivity of mineral trioxide aggregate in MC3T3-E1cells: Youngdan Jeong, Wonkyung Yang, Hyunjung Ko, Miri Kim; Restor Dent Endod 2014;39(3):187-194. Published online June 19, 2014; DOI: https://doi.org/10.5395/rde.2014.39.3.187

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Objectives

The effects of bone morphogenetic protein-2 (BMP-2) and enamel matrix derivative (EMD) respectively with mineral trioxide aggregate (MTA) on hard tissue regeneration have been investigated in previous studies. This study aimed to compare the osteogenic effects of MTA/BMP-2 and MTA/EMD treatment in MC3T3-E1 cells.

Materials and Methods

MC3T3-E1 cells were treated with MTA (ProRoot, Dentsply), BMP-2 (R&D Systems), EMD (Emdogain, Straumann) separately and MTA/BMP-2 or MTA/EMD combination. Mineralization was evaluated by staining the calcium deposits with alkaline phosphatase (ALP, Sigma-Aldrich) and Alizarin red (Sigma-Aldrich). The effects on the osteoblast differentiation were evaluated by the expressions of osteogenic markers, including ALP, bone sialoprotein (BSP), osteocalcin (OCN), osteopontin (OPN) and osteonectin (OSN), as determined by reverse-transcription polymerase chain reaction analysis (RT-PCR, AccuPower PCR, Bioneer).

Results

Mineralization increased in the BMP-2 and MTA/BMP-2 groups and increased to a lesser extent in the MTA/EMD group but appeared to decrease in the MTA-only group based on Alizarin red staining. ALP expression largely decreased in the EMD and MTA/EMD groups based on ALP staining. In the MTA/BMP-2 group, mRNA expression of OPN on day 3 and BSP and OCN on day 7 significantly increased. In the MTA/EMD group, OSN and OCN gene expression significantly increased on day 7, whereas ALP expression decreased on days 3 and 7 (p < 0.05).

Conclusions

These results suggest the MTA/BMP-2 combination promoted more rapid differentiation in MC3T3-E1 cells than did MTA/EMD during the early mineralization period.

Citations

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GelMA‐based hydrogel biomaterial scaffold: A versatile platform for regenerative endodontics
Lei Huang, Xuan Chen, XiaoXia Yang, Yinchun Zhang, Xiaoling Qiu
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Md. Niaj Morshed, Reshmi Akter, Imran Mahmud, Ah-Yeong Gwon, Jin Woo Jeang, Yeong-Geun Lee, Dae Won Park, Deok Chun Yang, Yeon Ju Kim, Se-Chan Kang
Life.2024; 15(1): 41. CrossRef
Anti-osteoporosis effects of triterpenoids from the fruit of sea buckthorn (Hippophae rhamnoides) through the promotion of osteoblast differentiation in mesenchymal stem cells, C3H10T1/2
Da Eun Lee, Kun Hee Park, Joo-Hyun Hong, Seon Hee Kim, Ki-Moon Park, Ki Hyun Kim
Archives of Pharmacal Research.2023; 46(9-10): 771. CrossRef
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Mijoo Kim, Marc Hayashi, Bo Yu, Thomas K. Lee, Reuben H. Kim, Deuk-Won Jo
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Nerve growth factor promotes osteogenic differentiation of MC3T3-E1 cells via BMP-2/Smads pathway
Xuming Yang, Donggang Mou, Qunying Yu, Jimei Zhang, Ying Xiong, Zhimin Zhang, Shan Xing
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Kun Hee Park, Joo-Hyun Hong, Seon-Hee Kim, Jin-Chul Kim, Ki Hyun Kim, Ki-Moon Park
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Ebrahim Patel, Priyamvada Pradeep, Pradeep Kumar, Yahya E. Choonara, Viness Pillay
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Nina Bono, Paolo Tarsini, Gabriele Candiani
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An assessment of the overexpression of BMP‐2 in transfected human osteoblast cells stimulated by mineral trioxide aggregate and Biodentine
E. M. Rodrigues, A. L. Gomes‐Cornélio, A. Soares‐Costa, L. P. Salles, M. Velayutham, C. Rossa‐Junior, J. M. Guerreiro‐Tanomaru, M. Tanomaru‐Filho
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Combined Effects of Growth Hormone and Mineral Trioxide Aggregate on Growth, Differentiation, and Angiogenesis in Human Dental Pulp Cells
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Combined effects of mineral trioxide aggregate and human placental extract on rat pulp tissue and growth, differentiation and angiogenesis in human dental pulp cells
Seok-Woo Chang, Ji-Youn Kim, Mi-Joo Kim, Ga-Hyun Kim, Jin-Kyu Yi, Deok-Won Lee, Kee-Yeon Kum, Eun-Cheol Kim
Acta Odontologica Scandinavica.2016; 74(4): 298. CrossRef
Mineral trioxide aggregate induces osteoblastogenesis via Atf6
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Tissue response of Pro-Root® MTA with rhBMP-2 in pulpotomized rat teeth: Kyungtae Park, Wonkyung Yang, Hyunjung Ko, Miri Kim; J Korean Acad Conserv Dent 2007;32(5):403-410. Published online September 30, 2007; DOI: https://doi.org/10.5395/JKACD.2007.32.5.403

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The purpose of this study was to investigate whether rhBMP-2 (BMP2) could induce synergistic effect with Pro-Root® MTA (MTA) in pulpotomized teeth in the rats. Healthy upper first molars from thirty-two, 10 weeks old, Sprague-Dawley rats were used for this investigation. The molars were exposed with round bur, and light pressure was applied with sterilized cotton to control hemorrhage. 1.2 grams of MTA cement was placed in right first molars as a control group. In left first molars, 1 µg of BMP2 was additionally placed on exposed pulps with MTA. All cavities were back-filled with light-cured glass-ionomer cements. The rats were sacrificed after 2 weeks and 7 weeks, respectively. Then histologic sections were made and assessed by light microscopy. Data were statistically analyzed via student t-test with SPSSWIN 12.0 program (p < 0.05).

Inflammation observed in 2 weeks groups were severe compared to the 7 weeks groups. But the differences were not statistically significant. BMP2-addition groups had less inflammation than MTA groups in both periods, though these differences were also not statistically significant. In conclusion, the combination of BMP2 and MTA showed no differences with MTA only for pulpotomy of rat teeth.

Citations

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A bioactivity study of Portland cement mixed with β-glycerophosphosphate on human pulp cell
Young-Hwan Oh, Young-Joo Jang, Yong-Bum Cho
Journal of Korean Academy of Conservative Dentistry.2009; 34(5): 415. CrossRef

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The Effect of Estrogen Deficiency on Rat Pulpodentinal Complex: Miri Kim, Won-Kyung Yang, Jin Baek, Jong-Jin Kim, Won-Kyung Kim, Young-Kyoo Lee; J Korean Acad Conserv Dent 2005;30(5):402-408. Published online January 14, 2005; DOI: https://doi.org/10.5395/JKACD.2005.30.5.402

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Abstract

The purpose of this study was to investigate the effects of estrogen deficiency on pulpodentinal complex of tooth in ovariectomized rats. Thirty female Sprague-Dawley rats, 10 weeks old, were used. Rats were grouped into two groups. One group (n = 15) was subjected to sham surgery (SHAM) and the other group (n = 15) was ovariectomized bilaterally (OVX). Animals were sacrificed 12 weeks later, and their mandibular molars and associated periodontal supporting tissues were dissected out, and fixed in 10% buffered formalin. For comparison of groups, immunostained for osteonectin. Histomorphometrical measurement of change of teeth was performed using an image analysis system and paired t-test was used and the level of significance for overall differences was set at p < 0.05.

In immunostaining of osteonectin, they were significantly different from each other. The predentin thickness in OVX rats was wider than in SHAM rats. And in SHAM rats, osteonectin was more specifically stained in predentin areas than in OVX rats. These results indicate that estrogen deficiency increased the unmineralized predentin areas and decreased osteonectin content in pulpal tissues in rats. If our result is applicable to human studies, odotoblast is affected by estrogen deficiency.

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Tissue reactions to implanted intracanal medicaments in rats: Miri Kim, Byunghyun Kim, Wonkyung Kim; J Korean Acad Conserv Dent 2004;29(1):23-29. Published online January 31, 2004; DOI: https://doi.org/10.5395/JKACD.2004.29.1.023

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