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- Original Article Histological evaluation of direct pulp capping with DSP-derived synthetic peptide in beagle dog
- Jae-Hoon Kim1, Jun-Bae Hong1, Bum-Soon Lim2, Byeong-Hoon Cho1,*
-
J Korean Acad Conserv Dent 2009;34(2):-129.
DOI: https://doi.org/10.5395/JKACD.2009.34.2.120
Published online: January 14, 2009
1Department of Conservative Dentistry, School of Dentistry, Seoul National University
2Department of Dental Biomaterials Science, School of Dentistry, Seoul National University
- *Corresponding Author: Byeong-Hoon Cho, Department of Conservative Dentistry, School of Dentistry, Seoul National University, 28 Yeongeon-dong, Jongro-gu, Seoul, 110-749, Korea Tel: +82-2-2072-3514 Fax: +82-2-2072-3859, E-mail: chobh@snu.ac.kr
• Received: November 28, 2008 • Revised: December 15, 2008 • Accepted: March 3, 2009
Copyright © 2009 The Korean Academy of Conservative Dentistry
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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- Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide
Yoon Lee, Yeoung-Hyun Park, Dong-Seol Lee, You-Mi Seo, Ji-Hyun Lee, Joo-Hwang Park, Han-Wool Choung, So-Hyun Park, Won Shon, Joo-Cheol Park
Materials.2020; 13(20): 4618. CrossRef - Pulp response of beagle dog to direct pulp capping materials: Histological study
Ji-Hyun Bae, Young-Gyun Kim, Pil-Young Yoon, Byeong-Hoon Cho, Yong-Hoon Choi
Journal of Korean Academy of Conservative Dentistry.2010; 35(1): 5. CrossRef
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XML DownloadHistological evaluation of direct pulp capping with DSP-derived synthetic peptide in beagle dog
Figure 1. Pulp capping with PEP at 2 weeks. Although the pulp capping material was impacted into the pulp, newly formed hard tissue barrier was observed. Incisal portion of the pulp from the impacted site (left portion in the figure) showed degeneration of the pulp.
Figure 2. Pulp capping with CH at 2 weeks. Newly formed hard tissue barrier was observed and odontoblast-like cells arranged beneath it. Although hemorrhage was seen, a few inflammatory cells were found.
Figure 3. Pulp capping with PEP+CH at 2 weeks. Hard tissue formation was rare. Inflammatory cells infiltrated around the impacted capping material.
Figure 4. Pulp capping with WMTA at 2 weeks. Among the dentin debris, scattered hard tissue was formed. Infiltration of inflammatory cells under odontoblast cell layer and dilatation of blood vessels were observed.
Figure 5. Pulp capping with PEP at 1 month. Many inflammatory cells infiltrated around the exposed site. There was no hard tissue formation even around the dentin debris.
Figure 6. Pulp capping with CH at 1 month. The thickness of hard tissue increased, compared with that of 2 weeks. In newly formed hard tissue, dentinal tubule-like structures were observed.
Figure 7. Pulp capping with PEP+CH at 1 month. Newly formed hard tissue grew mainly around the fragments of dentin. Dilatation of blood vessels was observed.
Figure 8. Pulp capping with WMTA at 1 month. Newly formed hard tissue grew into a complete bridge. They include the fragments of dentin that were pushed into the pulp during cavity preparation.
Figure 9. Pulp capping with PEP at 3 months. Inflammatory response persisted at the exposed site. The newly formed hard tissue were related to the dentin debris.
Figure 10. Pulp capping with CH at 3 months. There was a complete dentin bridge and normal pulp tissue organization beneath the exposed site. There were vacuole-like structure, cells and dentin chips inside a dentin bridge.
Figure 11. Pulp capping with PEP+CH at 3 months. No hard tissue formation was observed. Inflammatory response of the pulp persisted.
Figure 12. Pulp capping with WMTA at 3 months. There was a thick hard tissue barrier, that did not include a cell body and had no tubular structure, across the exposed site.
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Histological evaluation of direct pulp capping with DSP-derived synthetic peptide in beagle dog
| Group | Experimental period |
||
|---|---|---|---|
| 2 weeks | 1 month | 3 months | |
| PEP | 6 | 6 | 6 |
| CH | 6 | 6 | 6 |
| PEP+CH | 6 | 6 | 6 |
| WMTA | 6 | 6 | 6 |
| Grade | Characterization |
|---|---|
| 0 | None or a few scattered inflammatory cells present in the pulpal area corresponding to pulp |
| exposure, characteristic of normal tissue | |
| 1 | Slight inflammatory cell infiltration with polymorphonuclear or mononuclear leukocytes |
| 2 | Moderate inflammatory cell infiltration involving the coronal pulp |
| 3 | Severe inflammatory cell infiltration involving the coronal pulp or abscess present |
| Grade | Characterization |
|---|---|
| 0 | Absent |
| 1 | Modest hard tissue deposition beneath the exposed area |
| 2 | Moderate hard tissue deposition beneath the exposed area |
| 3 | Heavy hard tissue deposition beneath the exposed area, appearing as a complete dentin bridge |
| Group | Experimental period | Number of specimens | Inflammatory response |
Hard tissue formation |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | 0 | 1 | 2 | 3 | |||
| PEP | 2 weeks | 6 | 0 | 3 | 1 | 2 | 4 | 1 | 1 | 0 |
| 1 month | 6 | 0 | 2 | 2 | 2 | 6 | 0 | 0 | 0 | |
| 3 months | 5 | 0 | 3 | 2 | 0 | 4 | 1 | 0 | 0 | |
| CH | 2 weeks | 5 | 1 | 2 | 2 | 0 | 0 | 2 | 2 | 1 |
| 1 month | 6 | 4 | 1 | 1 | 0 | 1 | 1 | 1 | 3 | |
| 3 months | 6 | 2 | 2 | 2 | 0 | 2 | 0 | 2 | 2 | |
| PEP+CH | 2 weeks | 6 | 2 | 1 | 1 | 2 | 4 | 0 | 2 | 0 |
| 1 month | 6 | 1 | 4 | 1 | 0 | 2 | 3 | 1 | 0 | |
| 3 months | 6 | 1 | 1 | 1 | 3 | 5 | 1 | 0 | 0 | |
| WMTA | 2 weeks | 4 | 0 | 3 | 1 | 0 | 1 | 3 | 0 | 0 |
| 1 month | 6 | 3 | 2 | 0 | 1 | 1 | 1 | 2 | 2 | |
| 3 months | 5 | 4 | 1 | 0 | 0 | 2 | 1 | 0 | 2 | |
Table 1. Number of teeth and the experimental periods
Table 2. Criteria for grading inflammatory cell response
Table 3. Criteria for grading hard tissue formation
Table 4. Inflammatory responses and hard tissue formation with 4 experimental pulp capping materials at 3 observation intervals
