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Original Article Development of nano-fluid movement measuring device and its application to hydrodynamic analysis of dentinal fluid
In-Bog Lee, Min-Ho Kim, Sun-Young Kim, Juhea Chang, Byung-Hoon Cho, Ho-Hyun Son, Seung-Ho Back
2008;33(2):-147.
DOI: https://doi.org/10.5395/JKACD.2008.33.2.141
Published online: March 31, 2008

Department of Conservative Dentistry, School of Dentistry, Seoul National University, Korea.

Corresponding Author: In-Bog Lee. Department of Conservative Dentistry, School of Dentistry, Seoul National University, 275-1 Yeongeon-Dong, Jongno-Gu, Seoul 110-768, Korea. Tel: 82-2-2072-3953, Fax: 82-2-2072-3859, inboglee@snu.ac.kr
• Received: February 5, 2008   • Revised: March 3, 2008   • Accepted: March 8, 2008

Copyright © 2008 Korean Academy of Conservative Dentistry

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  • This study was aimed to develop an instrument for real-time measurement of fluid conductance and to investigate the hydrodynamics of dentinal fluid. The instrument consisted of three parts; (1) a glass capillary and a photo sensor for detection of fluid movement, (2) a servo-motor, a lead screw and a ball nut for tracking of fluid movement, (3) a rotary encoder and software for data processing.
    To observe the blocking effect of dentinal fluid movement, oxalate gel and self-etch adhesive agent were used. BisBlock (Bisco) and Clearfil SE Bond (Kuraray) were applied to the occlusal dentin surface of extracted human teeth. Using this new device, the fluid movement was measured and compared between before and after each agent was applied.
    The instrument was able to measure dentinal fluid movement with a high resolution (0.196 nL) and the flow occurred with a rate of 0.84 to 15.2 nL/s before treatment. After BisBlock or Clearfil SE Bond was used, the fluid movement was decreased by 39.8 to 89.6%.
  • Keywords: Dentinal tubule; Water conductance; Dentin hypersensitivity; Oxalate; Self etching adhesive; Hydrodynamics
Figure 1-a
Schematic diagram of the nano-flow measurement device.
jkacd-33-141-g001.jpg
Figure 1-b
Circuit diagram of photo sensor and servo amplifier.
jkacd-33-141-g002.jpg
Figure 2
Representative curves of dentinal fluid flow before and after application of BisBlock (a) or Clearfil SE bond (b).
jkacd-33-141-g003.jpg
Table 1
Water flow (nL) during 300 s and mean flow rate (nL/s) through dentin before and after application of BisBlock or Clearfil SE bond on the exposed dentin surface
jkacd-33-141-i001.jpg
  • 1. Roberson TM, Heymann HO, Swift EJ. Art and science of operative dentistry. 2002;4th edi. Mosby; 14-62.
  • 2. Summitt JB, Robbins JW, Schwartz RS. Fundamentals of operative dentistry. 2001;2nd edi. Quintessence publishing co, Inc; 8-22.
  • 3. Brännström M. Dentin and pulp in restorative dentistry. 1981;Wolfe medical.
  • 4. Hirata K, Nakashima M, Sekine I, Mukouyama Y, Kimura K. Dentinal fluid movement associated with loading of restorations. J Dent Res. 1991;70: 975-978.ArticlePubMedPDF
  • 5. Orchardson R, Cadden SW. An update on the physiology of the dentin-pulp complex. Dent Update. 2001;28: 200-209.ArticlePubMed
  • 6. Brännstorm M, Lindén L, Johnson G. Movement of dentinal and pulpal fluid caused by clinical procedures. J Dent Res. 1968;5: 679-682.
  • 7. Pashley DH, Derkson GD, Tao L, Kalathoor S. The effect of a multi-step dentin bonding system on dentin permeability. Dent Mater. 1988;4: 60-63.PubMed
  • 8. Sauro S, Pashley DH, Montanari , Chersoni S, Carvalho RM, Toledano M, Osorio R, Tay FR, Prati C. Effect of simulated pulpal pressure on dentin permeability and adhesion of self-etch adhesives. Dent Mater. 2007;23: 705-713.ArticlePubMed
  • 9. Paphangkorakit J, Osborn JW. The effect of normal occlusal forces on fluid movement through human dentin in vitro. Arch Oral Biol. 2000;45: 1033-1041.PubMed
  • 10. De La Macorra JC, Escribano NI. Comparison of two methods to measure permeability of dentin. J Biomed Mater Res. 2002;63: 531-534.ArticlePubMed
  • 11. Ozok AR, Wu MK, ten Cate JM, Wesselink PR. Effect of dentinal fluid composition on dentin demineralization in vitro. J Dent Res. 2004;83: 849-853.ArticlePubMedPDF
  • 12. Gregoire G, Joniot S, Guignes P, Millas A. Dentin permeability: self etching and one bottle dentin systems. J Prosthet Dent. 2003;90: 42-49.PubMed
  • 13. Pashley DH, Matthews WG, Zhang Y, Johnson M. Fluid shifts across human dentin in vitro in response to hydrodynamic stimuli. Arch Oral Biol. 1996;41: 1065-1072.PubMed
  • 14. Ciucchi B, Bouillaguet S, Holz J, Pashley D. Dentinal fluid dynamics in human teeth, In vivo. J Endod. 1995;21: 191-193.ArticlePubMed
  • 15. Kolker JL, Vargas MA, Armstrong SR, Dawson DV. Effect of desensitizing agents on dentin permeability and dentin tubule occlusion. J Adhes Dent. 2002;4: 211-221.PubMed
  • 16. Ratih DN, Palamara JEA, Messer HH. Dentinal fluid flow and cuspal displacement in response to resin composite restorative procedures. Dent Mater. 2007;23: 1405-1411.ArticlePubMed
  • 17. Ratih DN, Palamara JEA, Messer HH. Temperature change, dentinal fluid flow and cuspal displacement during resin composite restoration. J Oral Rehab. 2007;34: 693-701.Article
  • 18. Ciucchi B, Bouillaguet S, Delaloye M. Volume of the internal gap formed under composite restorations in vitro. J Dent. 1997;25: 305-312.ArticlePubMed
  • 19. Martin RL, Brackett WW, Loushine RJ, Rockman RA, Ferrari M, Pashley DH, Tay FR. Sealing properties of mineral trioxide aggregate orthograde apical plugs and root fillings in an in vitro apexification model. J Endod. 2007;33: 272-275.ArticlePubMed
  • 20. Beneson W, Harris JW, Stocken H, Lutz H. Handbook of physics. 2006;Springer; 153-210.
  • 21. Breschi L, Mazzoni A, Ruggeri A, Cadenaro M, Linarda RD, Dorigo EDS. Dental adhesion review: Aging and stability of bonded interface. Dent Mater. 2008;24: 90-101.PubMed
  • 22. Tay FR, Pashley DH. Water treeing-a potential mechanism for degradation of dentin adhesives. Am J Dent. 2003;16: 6-12.PubMed

Tables & Figures

Figure 1-a
Schematic diagram of the nano-flow measurement device.
jkacd-33-141-g001.jpg
Figure 1-b
Circuit diagram of photo sensor and servo amplifier.
jkacd-33-141-g002.jpg
Figure 2
Representative curves of dentinal fluid flow before and after application of BisBlock (a) or Clearfil SE bond (b).
jkacd-33-141-g003.jpg
Table 1
Water flow (nL) during 300 s and mean flow rate (nL/s) through dentin before and after application of BisBlock or Clearfil SE bond on the exposed dentin surface
jkacd-33-141-i001.jpg

REFERENCES

  • 1. Roberson TM, Heymann HO, Swift EJ. Art and science of operative dentistry. 2002;4th edi. Mosby; 14-62.
  • 2. Summitt JB, Robbins JW, Schwartz RS. Fundamentals of operative dentistry. 2001;2nd edi. Quintessence publishing co, Inc; 8-22.
  • 3. Brännström M. Dentin and pulp in restorative dentistry. 1981;Wolfe medical.
  • 4. Hirata K, Nakashima M, Sekine I, Mukouyama Y, Kimura K. Dentinal fluid movement associated with loading of restorations. J Dent Res. 1991;70: 975-978.ArticlePubMedPDF
  • 5. Orchardson R, Cadden SW. An update on the physiology of the dentin-pulp complex. Dent Update. 2001;28: 200-209.ArticlePubMed
  • 6. Brännstorm M, Lindén L, Johnson G. Movement of dentinal and pulpal fluid caused by clinical procedures. J Dent Res. 1968;5: 679-682.
  • 7. Pashley DH, Derkson GD, Tao L, Kalathoor S. The effect of a multi-step dentin bonding system on dentin permeability. Dent Mater. 1988;4: 60-63.PubMed
  • 8. Sauro S, Pashley DH, Montanari , Chersoni S, Carvalho RM, Toledano M, Osorio R, Tay FR, Prati C. Effect of simulated pulpal pressure on dentin permeability and adhesion of self-etch adhesives. Dent Mater. 2007;23: 705-713.ArticlePubMed
  • 9. Paphangkorakit J, Osborn JW. The effect of normal occlusal forces on fluid movement through human dentin in vitro. Arch Oral Biol. 2000;45: 1033-1041.PubMed
  • 10. De La Macorra JC, Escribano NI. Comparison of two methods to measure permeability of dentin. J Biomed Mater Res. 2002;63: 531-534.ArticlePubMed
  • 11. Ozok AR, Wu MK, ten Cate JM, Wesselink PR. Effect of dentinal fluid composition on dentin demineralization in vitro. J Dent Res. 2004;83: 849-853.ArticlePubMedPDF
  • 12. Gregoire G, Joniot S, Guignes P, Millas A. Dentin permeability: self etching and one bottle dentin systems. J Prosthet Dent. 2003;90: 42-49.PubMed
  • 13. Pashley DH, Matthews WG, Zhang Y, Johnson M. Fluid shifts across human dentin in vitro in response to hydrodynamic stimuli. Arch Oral Biol. 1996;41: 1065-1072.PubMed
  • 14. Ciucchi B, Bouillaguet S, Holz J, Pashley D. Dentinal fluid dynamics in human teeth, In vivo. J Endod. 1995;21: 191-193.ArticlePubMed
  • 15. Kolker JL, Vargas MA, Armstrong SR, Dawson DV. Effect of desensitizing agents on dentin permeability and dentin tubule occlusion. J Adhes Dent. 2002;4: 211-221.PubMed
  • 16. Ratih DN, Palamara JEA, Messer HH. Dentinal fluid flow and cuspal displacement in response to resin composite restorative procedures. Dent Mater. 2007;23: 1405-1411.ArticlePubMed
  • 17. Ratih DN, Palamara JEA, Messer HH. Temperature change, dentinal fluid flow and cuspal displacement during resin composite restoration. J Oral Rehab. 2007;34: 693-701.Article
  • 18. Ciucchi B, Bouillaguet S, Delaloye M. Volume of the internal gap formed under composite restorations in vitro. J Dent. 1997;25: 305-312.ArticlePubMed
  • 19. Martin RL, Brackett WW, Loushine RJ, Rockman RA, Ferrari M, Pashley DH, Tay FR. Sealing properties of mineral trioxide aggregate orthograde apical plugs and root fillings in an in vitro apexification model. J Endod. 2007;33: 272-275.ArticlePubMed
  • 20. Beneson W, Harris JW, Stocken H, Lutz H. Handbook of physics. 2006;Springer; 153-210.
  • 21. Breschi L, Mazzoni A, Ruggeri A, Cadenaro M, Linarda RD, Dorigo EDS. Dental adhesion review: Aging and stability of bonded interface. Dent Mater. 2008;24: 90-101.PubMed
  • 22. Tay FR, Pashley DH. Water treeing-a potential mechanism for degradation of dentin adhesives. Am J Dent. 2003;16: 6-12.PubMed

Citations

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    Development of nano-fluid movement measuring device and its application to hydrodynamic analysis of dentinal fluid
    J Korean Acad Conserv Dent. 2008;33(2):141-147.   Published online March 31, 2008
    DOI: https://doi.org/10.5395/JKACD.2008.33.2.141
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Development of nano-fluid movement measuring device and its application to hydrodynamic analysis of dentinal fluid
Image Image Image
Figure 1-a Schematic diagram of the nano-flow measurement device.
Figure 1-b Circuit diagram of photo sensor and servo amplifier.
Figure 2 Representative curves of dentinal fluid flow before and after application of BisBlock (a) or Clearfil SE bond (b).

Figure 1-a

Figure 1-b

Figure 2

Development of nano-fluid movement measuring device and its application to hydrodynamic analysis of dentinal fluid

Water flow (nL) during 300 s and mean flow rate (nL/s) through dentin before and after application of BisBlock or Clearfil SE bond on the exposed dentin surface
Table 1 Water flow (nL) during 300 s and mean flow rate (nL/s) through dentin before and after application of BisBlock or Clearfil SE bond on the exposed dentin surface

Table 1

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