The effect of canal filling with gutta-percha or resilon on Enterococcus faecalis in bovine dentinal tubules

Sang-Wook Jee; Euiseong Kim; Il-Young Jung; Yun-Jung Yoo

doi:10.5395/JKACD.2005.30.5.385

Articles

Page Path: HOME > Restor Dent Endod > Volume 30(5); 2005 > Article

Original Article The effect of canal filling with gutta-percha or resilon on Enterococcus faecalis in bovine dentinal tubules: Sang-Wook Jee¹, Euiseong Kim¹, Il-Young Jung¹, Yun-Jung Yoo²; Journal of Korean Academy of Conservative Dentistry 2005;30(5):385-392.
DOI: https://doi.org/10.5395/JKACD.2005.30.5.385
Published online: September 30, 2005

¹Department of Conservative Dentistry, Yonsei University, Korea.

²Department of Oral Biology, College of Dentistry, Yonsei University, Korea.

Corresponding author: Euiseong Kim. Department of Conservative Dentistry, College of Dentistry, Yonsei University, Sinchon-dong 134, Seodaemun-gu, Seoul, Korea 120-752. Tel: 82-2-2228-8700, andyendo@yumc.yonsei.ac.kr

• Received: February 22, 2005 • Revised: May 24, 2005 • Accepted: June 13, 2005

19 Views
0 Download

prev next

Full Article

Download PDF

Abstract
REFERENCES

Abstract

The purpose of this study was to observe the effect of canal filling on the bacteria left in the dentinal tubules and to compare the sealing ability between Gutta-percha and Resilon. The bovine dentin block models were prepared. E. faecalis was inoculated to dentin blocks and incubated. The dentin blocks were divided into 5 groups.

Group 1 was the negative control. Group 2 was the positive control. Group 3 was filled with ZOE based sealer and Gutta-percha, Group 4 with resin based sealer and Gutta-percha, and Group 5 with resin based sealer and Resilon. After 24 hour, the blocks were incubated at 37℃ for 1, 2, 3 and 4 weeks on BHI agar plates.

The internal dentin portion of the blocks was removed using ISO 027, 029, 031, 035 round burs and the dentin chips were incubated at 37℃ for 24 hour. Following incubation, the optical density of the medium was measured. The data were statistically analysed using repeated measures ANOVA and one-way ANOVA.

The results were as follows,

1. There was statistically significant reduction in the number of E. faecalis of the group where dentinal tubules were completely sealed with nail varnish in comparison with the groups obturated with gutta-percha or resilon (p < 0.05).

2. In group 5, the number of E. faecalis in the dentinal tubules decreased significantly with time (p < 0.05), whereas in Group 3 and 4, there was no reduction in its number (p > 0.05).

3. Under the conditions of this experiment, E. faecalis survived up to 4 weeks after obturation with gutta-percha or resilon (p > 0.05).
Keywords: Enterococcus faecalis; Resilon; Root canal sealing effect

REFERENCES

1. Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Pathol. 1965;20: 340-349.Article PubMed
2. Möller AJ, Fabricius L, Dahlen G, Ohman AE, Heyden G. Influence on periapical tissues of indigenous oral bacteria and necrotic pulp tissue in monkeys. Scand J Dent Res. 1981;89: 475-484.Article PubMed
3. Sundqvist G. Bacteriological studies of nectrotic dental pulps. Umea Univ Odontol Diss. 1976;7: 1-94 Thesis. Ref Type: Generic.
4. Byström A, Sundqvist G. Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy. Scand J Dent Res. 1981;89: 321-328.Article PubMed
5. Abou-Rass M, Piccinino MV. The effectiveness of four clinical irrigation methods on the removal of root canal debris. Oral Surg Oral Med Oral Pathol. 1982;54: 323-328.PubMed
6. Byström A, Claesson R, Sundqvist G. The antibacterial effect of camphorated paramonochlorophenol, camphorated phenol and calcium hydroxide in the treatment of infectedroot canals. Endod Dent Traumatol. 1985;1: 170-175.Article PubMed
7. Sjögren U, Figdor D, Spangberg L, Sundqvist G. The antimicrobial effect of calcium hydroxide as a short-term intracanal dressing. Int Endod J. 1991;24: 119-125.Article PubMed
8. Chong BS, Pitt Ford TR. The role of intracanal medication in root canal treatment. Int Endod J. 1992;25: 97-106.Article PubMed
9. Walton RE, Torabinejad M. Principles and Practice of Endodontics. 2002;3rd ed. W.B. Saunders company; 232-234.
10. Peters LB, van Winkelhoff AJ, Buijs JF, Wesselink PR. Effects of instrumentation, irrigation and dressing with calcium hydroxide on infection in pulpless teeth with periapical bone lesions. Int Endod J. 2002;35: 13-21.Article PubMed
11. Haapasalo HK, Siren EK, Waltimo TM, Orstavik D, Haapasalo MP. Inactivation of local root canal medicaments by dentine: an in vitro study. Int Endod J. 2000;33: 126-131.Article PubMed
12. Portenier I, Haapasalo H, Rye A, Waltimo T, Orstavik D, Haapasalo M. Inactivation of root canal medicaments by dentine, hydroxylapatite and bovine serum albumin. Int Endod J. 2001;34: 184-188.Article PubMed PDF
13. Ørstavik D, Haapasalo M. Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules. Endod Dent Traumatol. 1990;6: 142-149.Article PubMed
14. Estrela C, Pimenta FC, Ito IY, Bammann LL. Antimicrobial evaluation of calcium hydroxide in infected dentinal tubules. J Endod. 1999;25: 416-418.Article PubMed
15. Molander A, Reit C, Dahlen G, Kvist T. Microbiological status of root-filled teeth with apical periodontitis. Int Endod J. 1998;31: 1-7.Article PubMed
16. Sundqvist G, Figdor D. Microbiological analysis of teeth with failed endodontic treatment and the outcome of conservative retreatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;85: 86-93.PubMed
17. Evans M, Davies JK, Sundqvist G, Figdor D. Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide. Int Endod J. 2002;35: 221-228.Article PubMed
18. Kim SK, Kim YO. Influence of calcium hydroxide intracanal medication on apical seal. Int Endod J. 2002;35: 623-628.PubMed
19. Soltanoff W. A comparative study of the single-visit and the multiple-visit edodontic procedure. J Endod. 1978;4: 278-281.PubMed
20. Oliet S. Single-visit endodontics: a clinical study. J Endod. 1983;9: 147-152.Article PubMed
21. Peters LB, Wesselink PR. Periapical healing of endodontically treated teeth in one and two visits obturated in the presence or absence of detectable microorganisms. Int Endod J. 2002;35: 660-667.Article PubMed
22. Moorer WR, Genet JM. Evidence for antibacterial activity of endodontic gutta-percha cones. Oral Surg Oral Med Oral Pathol. 1982;53: 503-507.Article PubMed
23. Heling I, Chandler NP. The antimicrobial effect within dentinal tubules of four root canal sealers. J Endod. 1996;22: 257-259.Article PubMed
24. Kaplan AE, Picca M, Gonzalez MI, Macchi RL, Molgatini SL. Antimicrobial effect of six endodontic sealers: an in vitro evaluation. Endod Dent Traumatol. 1999;15: 42-45.Article PubMed
25. Fuss Z, Charniaque O, Pilo R, Weiss E. Effect of various mixing ratios on antibacterial properties and hardness of endodontic sealers. J Endod. 2000;26: 519-522.Article PubMed
26. Siqueira JF Jr, Favieri A, Gahyva SM, Moraes SR, Lima KC, Lopes HP. Antimicrobial activity and flow rate of newer and established root canal sealers. J Endod. 2000;26: 274-277.Article PubMed
27. Saleh IM, Ruyter IE, Haapasalo M, Orstavik D. Survival of Enterococcus faecalis in infected dentinal tubules after root canal filling with different root canal sealers in vitro. Int Endod J. 2004;37: 193-198.Article PubMed
28. Peters LB, Wesselink PR, Moorer WR. The fate and the role of bacteria left in root dentinal tubules. Int Endod J. 1995;28: 95-99.Article PubMed
29. Weiger R, Rosendahl R, Lost C. Influence of calcium hydroxide intracanal dressings on the prognosis of teeth with endodontically induced periapical lesions. Int Endod J. 2000;33: 219-226.Article PubMed
30. Swanson K, Madison S. An evaluation of coronal microleakage in endodontically treated teeth. Part I. Time periods. J Endod. 1987;13: 56-59.PubMed
31. Torabinejad M, Ung B, Kettering JD. In vitro bacterial penetration of coronally unsealed endodontically treated teeth. J Endod. 1990;16: 566-569.Article PubMed
32. Khayat A, Lee SJ, Torabinejad M. Human saliva penetration of coronally unsealed obturated root canals. J Endod. 1993;19: 458-461.Article PubMed
33. Magura ME, Kafrawy AH, Brown CE Jr, Newton CW. Human saliva coronal microleakage in obturated root canals: an in vitro study. J Endod. 1991;17: 324-331.Article PubMed
34. Gish SP, Drake DR, Walton RE, Wilcox L. bacterial penetration through obturated canals following post preparation. J Am Dent Assoc. 1994;125: 1369-1372.PubMed
35. Ingle JI. Endodontics. 2002;4th ed. Hamilton: BC Decker Inc; 752-753.
36. Tidmarsh BG. Acid-cleansed and resin-sealed root canals. J Endod. 1978;4: 117-121.Article PubMed
37. Leonard JE, Gutmann JL, Guo IY. Apical and coronal seal of roots obturated with a dentine bonding agent and resin. Int Endod J. 1996;29: 76-83.Article PubMed
38. Mannocci F, Ferrari M. Apical seal of roots obturated with laterally condensed gutta-percha, epoxy resin cement, and dentin bonding agent. J Endod. 1998;24: 41-44.Article PubMed
39. Mounce R, Glassman G. Bonded endodontic obturation: Another quantum leap forward for endodontics. Oral Health. 2004;94(6):13-22.
40. Shipper G, Orstavik D, Teixeira FB, Trope M. An evaluation of microbial leakage in roots filled with a thermoplastic synthetic polymer-based root canal filling material (Resilon). J Endod. 2004;30: 342-347.Article PubMed
41. Teixeira FB, Teixeira EC, Thompson JY, Trope M. Fracture resistance of roots endodontically treated with a new resin filling material. J Am Dent Assoc. 2004;135: 646-652.Article PubMed
42. Hata G, Kawazoe S, Toda T, Weine FS. Sealing ability of Thermafil with and without sealer. J Endod. 1992;18: 322-326.Article PubMed
43. Orstavik D. Weight loss of endodontic sealers, cements and pastes in water. Scand J Dent Res. 1983;91: 316-319.Article PubMed
44. Peters DD. Two-year in vitro solubility evaluation of four Gutta-percha sealer obturation techniques. J Endod. 1986;12: 139-145.Article PubMed
45. Tronstad L, Barnett F, Flax M. Solubility and biocompatibility of calcium hydroxide-containing root canal sealers. Endod Dent Traumatol. 1988;4: 152-159.Article PubMed
46. Kontakiotis EG, Wu MK, Wesselink PR. Effect of sealer thickness on long-term sealing ability: a 2-year follow-up study. Int Endod J. 1997;30: 307-312.Article PubMed
47. Wu MK, Ozok AR, Wesselink PR. Sealer distribution in root canals obturated by three techniques. Int Endod J. 2000;33: 340-345.PubMed
48. Nair PN, Sjogren U, Krey G, Kahnberg KE, Sundqvist G. Intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscopic follow-up study. J Endod. 1990;16: 580-588.Article PubMed

Figure 1

Dentin disk block.

Figure 2

Schematic illustration of incubating dentin block on BHI agar.

Figure 3

The change of optical density with time.

^*significant difference statistically (p < 0.05).

Table 1

Experimental groups by canal filling materials (n = 32)

Table 2

E. faecalis left in dentinal tubules

Data were expressed as optical density of culture media of dentin chip.

Data description form: mean (s.d.).

^*: p-value by repeated measures ANOVA with time.

^†: statistically significant by repeated measures ANOVA.

^**: p-value by one-way ANOVA among groups at given experimental time.

a, b: multiple comparison using tukey method.

Tables & Figures

REFERENCES

Citations

Citations to this article as recorded by

ePub Link

Cite

CITE: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format: