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Research Article
Cytotoxicity of newly developed pozzolan cement and other root-end filling materials on human periodontal ligament cell
Minju Song, Tae-Sun Yoon, Sue-Youn Kim, Euiseong Kim
Restor Dent Endod 2014;39(1):39-44.   Published online January 20, 2014
DOI: https://doi.org/10.5395/rde.2014.39.1.39
AbstractAbstract PDFPubReaderePub
Objectives

The purpose of this study was to evaluate in vitro cytotoxicity of the pozzolan cement and other root-end filling materials using human periodontal ligament cell.

Materials and Methods

Endocem (Maruchi), white ProRoot MTA (Dentsply), white Angelus MTA (Angelus), and Super EBA (Bosworth Co.) were tested after set completely in an incubator at 37℃ for 7 days, Endocem was tested in two ways: 1) immediately after mixing (fresh specimens) and 2) after setting completely like other experimental materials. The methods for assessment included light microscopic examination, cell counting and WST-1 assay on human periodontal ligament cell.

Results

In the results of microscopic examination and cell counting, Super EBA showed significantly lower viable cell than any other groups (p < 0.05). As the results of WST-1 assay, compared with untreated control group, there was no significant cell viability of the Endocem group. However, the fresh mixed Endocem group had significantly less cell viability. The cells exposed to ProRoot MTA and Angelus MTA showed the highest viability, whereas the cells exposed to Super EBA displayed the lowest viability (p < 0.05).

Conclusions

The cytotoxicity of the pozzolan cement (Endocem) was comparable with ProRoot MTA and Angelus MTA. Considering the difficult manipulation and long setting time of ProRoot MTA and Angelus MTA, Endocem can be used as the alternative of retrofilling material.

Citations

Citations to this article as recorded by  
  • Effects of Three Retrograde Filling Materials on Production of Inflammatory Cytokines and Resorbing Mediators
    Samaneh Arab, Marjan Bahraminasab, Masoumeh Motamedi, Jamshid Hadjati, Alaviye Vahid
    Journal of Microbiota.2024;[Epub]     CrossRef
  • Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material
    Kazumasa Murata, Ayako Washio, Takahiko Morotomi, Thira Rojasawasthien, Shoichiro Kokabu, Chiaki Kitamura
    Nanomaterials.2021; 11(7): 1828.     CrossRef
  • Cell migration and osteo/odontogenesis stimulation of iRoot FS as a potential apical barrier material in apexification
    Y. Liu, X. M. Liu, J. Bi, S. Yu, N. Yang, B. Song, X. Chen
    International Endodontic Journal.2020; 53(4): 467.     CrossRef
  • Biocompatibility of Biodentine™ ® with Periodontal Ligament Stem Cells: In Vitro Study
    Duaa Abuarqoub, Nazneen Aslam, Hanan Jafar, Zakariya Abu Harfil, Abdalla Awidi
    Dentistry Journal.2020; 8(1): 17.     CrossRef
  • A micro-computed tomographic study of remaining filling materials of two bioceramic sealers and epoxy resin sealer after retreatment
    KyungJae Kim, Da Vin Kim, Sin-Young Kim, SungEun Yang
    Restorative Dentistry & Endodontics.2019;[Epub]     CrossRef
  • Comparison of Gap Volume after Retrofilling Using 4 Different Filling Materials: Evaluation by Micro–computed Tomography
    Sue Youn Kim, Hyeon-Cheol Kim, Su-Jung Shin, Euiseong Kim
    Journal of Endodontics.2018; 44(4): 635.     CrossRef
  • Anti-inflammatory and Mineralization Effects of ProRoot MTA and Endocem MTA in Studies of Human and Rat Dental Pulps In Vitro and In Vivo
    Do-Hee Kim, Ji-Hyun Jang, Bin-Na Lee, Hoon-Sang Chang, In-Nam Hwang, Won-Mann Oh, Sun-Hun Kim, Kyung-San Min, Jeong-Tae Koh, Yun-Chan Hwang
    Journal of Endodontics.2018; 44(10): 1534.     CrossRef
  • Effects of Three Calcium Silicate Cements on Inflammatory Response and Mineralization-Inducing Potentials in a Dog Pulpotomy Model
    Chung-Min Kang, Jiwon Hwang, Je Seon Song, Jae-Ho Lee, Hyung-Jun Choi, Yooseok Shin
    Materials.2018; 11(6): 899.     CrossRef
  • Cytocompatibility of Biodentine and iRoot FS with human periodontal ligament cells: an in vitro study
    T. Luo, J. Liu, Y. Sun, Y. Shen, L. Zou
    International Endodontic Journal.2018; 51(7): 779.     CrossRef
  • Biological response of commercially available different tricalcium silicate-based cements and pozzolan cement
    Serhat Köseoğlu, Tuğba Pekbağryank, Ebru Kucukyilmaz, Mehmet Sağlam, Sukru Enhos, Ayşe Akgün
    Microscopy Research and Technique.2017; 80(9): 994.     CrossRef
  • Biological efficacy of two mineral trioxide aggregate (MTA)-based materials in a canine model of pulpotomy
    Myeongyeon LEE, Chung-Min KANG, Je Seon SONG, Yooseok SHIN, Seunghye KIM, Seong-Oh KIM, Hyung-Jun CHOI
    Dental Materials Journal.2017; 36(1): 41.     CrossRef
  • Cytotoxicities and genotoxicities of cements based on calcium silicate and of dental formocresol
    Hyunjung Ko, Youngdan Jeong, Miri Kim
    Mutation Research/Genetic Toxicology and Environmental Mutagenesis.2017; 815: 28.     CrossRef
  • A Randomized Controlled Study of Mineral Trioxide Aggregate and Super Ethoxybenzoic Acid as Root-end Filling Materials in Endodontic Microsurgery: Long-term Outcomes
    Sunil Kim, Minju Song, Su-Jung Shin, Euiseong Kim
    Journal of Endodontics.2016; 42(7): 997.     CrossRef
  • Effects of two fast-setting calcium-silicate cements on cell viability and angiogenic factor release in human pulp-derived cells
    Chooryung J. Chung, Euiseong Kim, Minju Song, Jeong-Won Park, Su-Jung Shin
    Odontology.2016; 104(2): 143.     CrossRef
  • Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials
    Diana María Escobar-García, Eva Aguirre-López, Verónica Méndez-González, Amaury Pozos-Guillén
    BioMed Research International.2016; 2016: 1.     CrossRef
  • In Vitro Cytotoxicity Evaluation of Three Root-End Filling Materials in Human Periodontal Ligament Fibroblasts
    Hernán Coaguila-Llerena, Abraham Vaisberg, Zulema Velásquez-Huamán
    Brazilian Dental Journal.2016; 27(2): 187.     CrossRef
  • Dynamic intratubular biomineralization following root canal obturation with pozzolan‐based mineral trioxide aggregate sealer cement
    Yeon‐Jee Yoo, Seung‐Ho Baek, Kee‐Yeon Kum, Won‐Jun Shon, Kyung‐Mi Woo, WooCheol Lee
    Scanning.2016; 38(1): 50.     CrossRef
  • A Randomized Controlled Study of the Use of ProRoot Mineral Trioxide Aggregate and Endocem as Direct Pulp Capping Materials
    Minju Song, Minji Kang, Hyeon-Cheol Kim, Euiseong Kim
    Journal of Endodontics.2015; 41(1): 11.     CrossRef
  • Comparative Analysis of Selected Physicochemical Properties of Pozzolan Portland and MTA-Based Cements
    Maura Cristiane Gonçales Orçati Dorileo, Ricardo Dalla Villa, Orlando Aguirre Guedes, Andreza Maria Fábio Aranha, Alex Semenoff-Segundo, Matheus Coelho Bandeca, Alvaro Henrique Borges
    International Scholarly Research Notices.2014; 2014: 1.     CrossRef
  • Surgical endodontics: past, present, and future
    James L. Gutmann
    Endodontic Topics.2014; 30(1): 29.     CrossRef
  • Comparative analysis of physicochemical properties of root perforation sealer materials
    Maura Cristiane Gonçales Orçati Dorileo, Fábio Luis Miranda Pedro, Matheus Coelho Bandeca, Orlando Aguirre Guedes, Ricardo Dalla Villa, Alvaro Henrique Borges
    Restorative Dentistry & Endodontics.2014; 39(3): 201.     CrossRef
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Basic Research
The effect of several root-end filling materials on MG63 osteoblast-like cells
Jeong-Ho Lee, Won-Jun Shon, WooCheol Lee, Seung-Ho Baek
J Korean Acad Conserv Dent 2010;35(3):222-228.   Published online May 31, 2010
DOI: https://doi.org/10.5395/JKACD.2010.35.3.222
AbstractAbstract PDFPubReaderePub

The purpose of this study was to compare mineral trioxide aggregate (MTA; Dentsply, Tulsa Dental, Tulsa, OK, USA), which is widely used as root-end filling material, with DiaRoot BioAggregate (DB; Innovative BioCaramix Inc, Vancouver, BC, Canada), newly developed product, by using MG63 osteoblast-like cells. MTA, DB, and Intermediate Restorative Material (IRM; Dentsply Caulk, Milford, DE, USA) were used for root-end filling material while tissue culture plastic was used for control group. Each material was mixed and, the mixtures were left to set for 24 hours. MG63 cells were seeded to each group and then they were cultured for attachment for 4 hours. Following the attachment of cells to the root-end filling material, early cellular response was observed. After another 12 hours'culture, the level of attachment between cells and material was observed and in order to identify the effect of each material to bone formation, transforming growth factor beta1 (TGFβ1) and osteocalin (OC) were estimated by using enzyme-linked immunosorbent assay (ELISA), and the amount of alkaline phosphatase (ALP) was also measured. The data were analyzed using one-way ANOVA. As a result, only at OC and the number of cells which were attached to materials, there was no statistical difference between MTA and DB. At other items, there was statistically significant difference in all groups. Although DB has not shown exactly the same cellular response like that of MTA, the number of attached cells shows that biocompatibility of the material and OC indicates bone formation rate. Therefore, if DB is used for root end filling material, it is expected to lead to similar results to MTA.

Citations

Citations to this article as recorded by  
  • Comparative analysis of physicochemical properties of root perforation sealer materials
    Maura Cristiane Gonçales Orçati Dorileo, Fábio Luis Miranda Pedro, Matheus Coelho Bandeca, Orlando Aguirre Guedes, Ricardo Dalla Villa, Alvaro Henrique Borges
    Restorative Dentistry & Endodontics.2014; 39(3): 201.     CrossRef
  • Biocompatibility of root-end filling materials: recent update
    Payal Saxena, Saurabh Kumar Gupta, Vilas Newaskar
    Restorative Dentistry & Endodontics.2013; 38(3): 119.     CrossRef
  • 178 View
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  • 2 Crossref
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Original Articles
Apical microleakage of MTA with 4-META/MMA & TBB resin as a root-end filling material
Jin-Cheol Kim, Mi-Ri Kim, Hyun-Jung Ko, Won-Kyung Yang
J Korean Acad Conserv Dent 2009;34(4):371-376.   Published online July 31, 2009
DOI: https://doi.org/10.5395/JKACD.2009.34.4.371
AbstractAbstract PDFPubReaderePub

We evaluated in vitro microleakage of Mineral Trioxide Aggregate (MTA) powder with 4-methacryloxyethyl trimellitate anhydride (4-META) / methyl methacrylate (MMA) & tri-n-butylborane (TBB) resin as a retrograde filling material by using methylene blue dye method.

Fifty-two single rooted, extracted teeth were instrumented and obturated with gutta percha and AH plus sealer. The apical 3mm of each root was resected and 3mm deep ultrasonic root end preparation was done. External surface of roots was coated with nail varnish. Prepared teeth were randomly divided into five groups; Negative control: completely covered with nail varnish; Positive control: coated with nail varnish except for apical foramen; Group 1 (retrofilled with Portland cement); Group 2 (retrofilled with MTA); Group 3 (retrofilled with MTA powder mixed with 4-META/MMA & TBB resin). Immediately after completion of root-end filling, all specimens were submerged in methylene blue dye for 72 hours in 37℃ incubator. The roots were longitudinally sectioned and measured for extent of dye penetration by three different examiners under microscope (×10). The results were statistically analyzed using one way ANOVA and Turkey's HSD test. No leakage was evident in negative control and complete leakage in positive control group. Group 3 showed significantly less leakage than group 1 and 2 (p < 0.01). There was no significant difference between group 1 and 2 (p > 0.01).

It was concluded that MTA powder with 4-META/MMA & TBB resin was excellent in reducing initial apical microleakage.

Citations

Citations to this article as recorded by  
  • Characteristics of novel root-end filling material using epoxy resin and Portland cement
    Sang-Jin Lee, Jin Chung, Hee-Sam Na, Eun-Joo Park, Hyo-Jin Jeon, Hyeon-Cheol Kim
    Clinical Oral Investigations.2013; 17(3): 1009.     CrossRef
  • Sealing Ability of Three Different Materials Used as Retrograde Filling
    Ji-Hoon Park, Seung-Bok Kang, Yong-Hoon Choi, Ji-Hyun Bae
    Journal of Korean Dental Science.2012; 5(2): 60.     CrossRef
  • Physical properties of novel composite using Portland cement for retro-filling material
    Sang-Jin Lee, Ok-In Cho, Jiwan Yum, Jeong-Kil Park, Bock Hur, Hyeon-Cheol Kim
    Journal of Korean Academy of Conservative Dentistry.2010; 35(6): 445.     CrossRef
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A comparative study on radiopacity of canal filling and retrograde root-end filling materials
Yong-Sang Kim, Seo-Kyong Kim, Yun-Chan Hwang, In-Nam Hwang, Won-Mann Oh
J Korean Acad Conserv Dent 2008;33(2):107-114.   Published online March 31, 2008
DOI: https://doi.org/10.5395/JKACD.2008.33.2.107
AbstractAbstract PDFPubReaderePub

This study was performed to assess the radiopacity of a variety of canal filling and retrograde root-end filling materials according to the specification concerning root canal obturation materials.

Ten materials including Gutta-percha pellets, amalgam, Fuji II LC, Dyract® AP, Super EBA®, IRM®, AH 26®, Sealapex™, Tubli-Seal™ and dentin were evaluated in this study. In the first part, densitometric reading of an each step of aluminum step wedge on occlusal film were performed at 60 kVp (0.2, 0.3, 0.4 s), 70 kVp (0.2, 0.3, 0.33 s) to decide appropriate voltage and exposure time. In the second part, ten specimens which are 5 mm in diameter and 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mm in thickness, were fabricated from each material studied. The specimens were radiographed simultaneously with an aluminum step wedge under decided condition (60 kVp, 0.2 s). The mean radiographic density values of the materials were transformed into radiopacity expressed equivalent thickness of aluminum (mm Al).

The following results were obtained.

Among the various conditions including 0.2 s, 0.3 s, 0.4 s at 60 kVp and 0.2 s, 0.3 s, 0.33 s at 70 kVp, the appropriate voltage and exposure time that meet the requirement of density from 0.5 to 2.0 was 0.2 s at 60 kVp.

All of the materials in this study had greater radiopacity than the minimun level recommended by ISO No. 4049 standards.

Most of the materials had greater radiopacity than 3 mm Al requirement of ANSI/ADA specification No. 57 (2000) and ISO No. 6876 (2001) standards except for Fuji II LC and Dyract.

It suggests that all experimental canal filling and retrograde root-end filling materials have a sufficient radiopacity that meet the requirement concerning root canal obturation materials except for Fuji II LC and Dyract.

Citations

Citations to this article as recorded by  
  • Evaluation of prognosis related to compliance with supportive periodontal treatment in patients with chronic periodontitis: a clinical retrospective study
    Jong-Bin Lee, Hye-Jung Shin, Dae-Yeob Kim, Eun-Kyoung Pang
    Journal of Periodontal & Implant Science.2019; 49(2): 76.     CrossRef
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Spectrophotometric evaluation of sealing effects of several root-end filling materials
Jin-Gyu Yi, Sang-Jin Park, Kyung-Kyu Choi, Gi-Woon Choi
J Korean Acad Conserv Dent 2003;28(6):449-456.   Published online November 30, 2003
DOI: https://doi.org/10.5395/JKACD.2003.28.6.449
AbstractAbstract PDFPubReaderePub

The purpose of this study is to evaluate the sealing effect of several root-end filling materials using spectrophotometric analysis. 180 single root teeth with one canal were instrumented and canal filled. Root resected and root end preparation was made. Teeth were randomly classified to 5 experimental group(MTA, EBA, IRM, TCP, ZOE) and 1 control group according to root-end filling material MTA group used PRO ROOT MTA, EBA group used Super EBA, TCP group used NEW APATITE LINER TYPE II main component of which is α-tricalcium phosphate(TCP). According to manufacture's instruction experimental material was mixed and retrfilled. After 2% methylene blue solution penetration absorbance for each test sample was measured with spectrophotometer (JASCO UV-530, Japan).

The mean absorbance of control and experimental group was as follows;

MTA : 0.092, IRM : 0.226, Super EBA : 0.255, ZOE : 0.374, Control : 0.425, TCP : 0.501 and the result analyzed by Turkey test at P=0.05 level.

Conclusions of this study are as follows;

The absorbance increase in follwing sequence MTA, IRM, Super EBA, ZOE, Control, TCP.

MTA showed the least leakage but was not significant with IRM or Super EBA and was significant with control or TCP(p<0.05).

TCP had the most leakage and was not significant with control group.

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