Restorative Dentistry & Endodontics

Research Article

Biocompatibility of two experimental scaffolds for regenerative endodontics: Dephne Jack Xin Leong, Frank C. Setzer, Martin Trope, Bekir Karabucak; Restor Dent Endod 2016;41(2):98-105. Published online March 28, 2016; DOI: https://doi.org/10.5395/rde.2016.41.2.98

Objectives

The biocompatibility of two experimental scaffolds for potential use in revascularization or pulp regeneration was evaluated.

Materials and Methods

One resilient lyophilized collagen scaffold (COLL), releasing metronidazole and clindamycin, was compared to an experimental injectable poly(lactic-co-glycolic) acid scaffold (PLGA), releasing clindamycin. Human dental pulp stem cells (hDPSCs) were seeded at densities of 1.0 × 10⁴, 2.5 × 10⁴, and 5.0 × 10⁴. The cells were investigated by light microscopy (cell morphology), MTT assay (cell proliferation) and a cytokine (IL-8) ELISA test (biocompatibility).

Results

Under microscope, the morphology of cells coincubated for 7 days with the scaffolds appeared healthy with COLL. Cells in contact with PLGA showed signs of degeneration and apoptosis. MTT assay showed that at 5.0 × 10⁴ hDPSCs, COLL demonstrated significantly higher cell proliferation rates than cells in media only (control, p < 0.01) or cells co-incubated with PLGA (p < 0.01). In ELISA test, no significant differences were observed between cells with media only and COLL at 1, 3, and 6 days. Cells incubated with PLGA expressed significantly higher IL-8 than the control at all time points (p < 0.01) and compared to COLL after 1 and 3 days (p < 0.01).

Conclusions

The COLL showed superior biocompatibility and thus may be suitable for endodontic regeneration purposes.

Citations

Citations to this article as recorded by

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Parisa Noohi, Mohammad J. Abdekhodaie, Mohammad H. Nekoofar, Kerstin M. Galler, Paul M. H. Dummer
International Endodontic Journal.2022; 55(12): 1277. CrossRef
In vitro performance of a nanobiocomposite scaffold containing boron-modified bioactive glass nanoparticles for dentin regeneration
Reza Moonesi Rad, Engin Pazarçeviren, Elif Ece Akgün, Zafer Evis, Dilek Keskin, Sıla Şahin, Ayşen Tezcaner
Journal of Biomaterials Applications.2019; 33(6): 834. CrossRef
Biological effects of silk fibroin 3D scaffolds on stem cells from human exfoliated deciduous teeth (SHEDs)
M. Collado-González, M. P. Pecci-Lloret, D. García-Bernal, S. Aznar-Cervantes, R. E. Oñate-Sánchez, J. M. Moraleda, J. L. Cenis, F. J. Rodríguez-Lozano
Odontology.2018; 106(2): 125. CrossRef
Pulp Regeneration Concepts for Nonvital Teeth: From Tissue Engineering to Clinical Approaches
Valérie Orti, Pierre-Yves Collart-Dutilleul, Sofía Piglionico, Orsolya Pall, Frédéric Cuisinier, Ivan Panayotov
Tissue Engineering Part B: Reviews.2018; 24(6): 419. CrossRef
Investigation of Human Dental Pulp Cells on a Potential Injectable Poly(lactic-co-glycolic acid) Microsphere Scaffold
Huiru Zou, Guanhua Wang, Fang Song, Xudong Shi
Journal of Endodontics.2017; 43(5): 745. CrossRef
Biocompatibility of hydrogel-based scaffolds for tissue engineering applications
Sheva Naahidi, Mousa Jafari, Megan Logan, Yujie Wang, Yongfang Yuan, Hojae Bae, Brian Dixon, P. Chen
Biotechnology Advances.2017; 35(5): 530. CrossRef

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Case Reports

Treatment of non-vital immature teeth with amoxicillin-containing triple antibiotic paste resulting in apexification: Hyon-Beom Park, Bin-Na Lee, Yun-Chan Hwang, In-Nam Hwang, Won-Mann Oh, Hoon-Sang Chang; Restor Dent Endod 2015;40(4):322-327. Published online August 28, 2015; DOI: https://doi.org/10.5395/rde.2015.40.4.322

Abstract PDF PubReader ePub

A recent treatment option for non-vital immature teeth in young patients is revascularization with triple antibiotic paste (TAP). However, tooth discoloration was reported with the use of conventional minocycline-containing TAP. In this case report, amoxicillin-containing TAP was used for revascularization of non-vital immature teeth to prevent tooth discoloration. At the 1 yr follow up, the teeth were asymptomatic on clinical examination and showed slight discoloration of the crown due to mineral trioxide aggregate (MTA) filling rather than amoxicillin-containing TAP. Radiographic examination revealed complete resolution of the periapical radiolucency, and closed apex with obvious periodontal ligament space. However, the root growth was limited, and the treatment outcome was more like apexification rather than revascularization. These results may be due to unstable blood clot formation which could not resist the condensation force of MTA filling, whether or not a collagen matrix was in place. These cases showed that although revascularization was not successful, apexification could be expected, resulting in the resolution of the periapical radiolucency and the closure of the apex. Therefore, it is worthwhile attempting revascularization of non-vital immature teeth in young patients.

Citations

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Comparative evaluation of effect of modified triple antibiotic paste and calcium hydroxide as intracanal medicament on microhardness of root dentin: An in vitro study
Aparna Palekar, Piyush Mantri, Minal Awinashe, Basawaraj Biradar, Mukund Singh
Endodontology.2024;[Epub] CrossRef
Healing of Large Endodontic Lesions Using Long-Term Application of a New Combination of Triple Antibiotics: A Series of Cases
Saeed Asgary, Ardavan Parhizkar, Maria Beatriz Duarte Gavião
Case Reports in Dentistry.2023; 2023: 1. CrossRef
Comparative Evaluation of Two Antibiotic Pastes for Root Canal Disinfection
Sadhna Sharma, Urvashi Bhushan, Mridula Goswami, CP Baveja
International Journal of Clinical Pediatric Dentistry.2022; 15(S1): S12. CrossRef
Regenerative Endodontics as the Future Treatment of Immature Permanent Teeth
Justyna Zbańska, Katarzyna Herman, Piotr Kuropka, Maciej Dobrzyński
Applied Sciences.2021; 11(13): 6211. CrossRef
Antimicrobial efficacy of triple antibiotic paste in teeth with primary endodontic infection: A systematic review
Rhythm Bains, Aseem P. Tikku, Promila Verma, Pragya Pandey
Asian Journal of Oral Health and Allied Sciences.2021; 11: 2. CrossRef
Effectiveness of MTA apical plug in dens evaginatus with open apices
Khoa Van Pham, Thu Anh Tran
BMC Oral Health.2021;[Epub] CrossRef
Lesion Sterilization and Tissue Repair: A Literature Review
Ankit Rawat, Jyoti Nagpal, Shreeya Mehta, Divya Vyas, Abhishek Kumar, Fathima Amal
Journal of Research and Advancement in Dentistry.2021; 12(3): 6. CrossRef
Spectrophotometric assessment of Tooth discoloration induced by various Antibiotic pastes
Ravi Gupta, Radhika Kewalramani, Dishant Patel
Research Journal of Pharmacy and Technology.2021; : 1979. CrossRef
Comparative evaluation of calcium release of the apical plugs formed by mineral trioxide aggregate, Biodentine, and EndoSequence root repair material with and without 2% triple antibiotic powder: An in vitro study
PoojaNitin Mapara, ND Shashikiran, Sachin Gugawad, Namrata Gaonkar, Savita Hadakar, Swapnil Taur, Dhanshri Khade
Journal of Indian Society of Pedodontics and Preventive Dentistry.2020; 38(2): 132. CrossRef
Effect of triple antibiotic loaded apatitic nanocarriers on Enterococcus faecalis biofilm – An In vitro study
S. Nagarathinam, V. Sujatha, K. Madhumathi, S. Mahalaxmi, P.Pranav Vanajassun, T.S.Sampath Kumar
Journal of Drug Delivery Science and Technology.2019; 51: 499. CrossRef
Coronal tooth discoloration induced by regenerative endodontic treatment using different scaffolds and intracanal coronal barriers: a 6-month ex vivo study
Noushin Shokouhinejad, Hassan Razmi, Maryam Farbod, Marzieh Alikhasi, Josette Camilleri
Restorative Dentistry & Endodontics.2019;[Epub] CrossRef
Triple antibiotic paste: momentous roles and applications in endodontics: a review
Ardavan Parhizkar, Hanieh Nojehdehian, Saeed Asgary
Restorative Dentistry & Endodontics.2018;[Epub] CrossRef
Mineral trioxide aggregate and other bioactive endodontic cements: an updated overview – part II: other clinical applications and complications
M. Torabinejad, M. Parirokh, P. M. H. Dummer
International Endodontic Journal.2018; 51(3): 284. CrossRef
Alternative to Avoid Tooth Discoloration after Regenerative Endodontic Procedure: A Systematic Review
Luciane Geanini Pena dos Santos, Luiz Alexandre Chisini, Camila Guerner Springmann, Beatriz Dulcineia Mendes de Souza, Fernanda Geraldo Pappen, Flávio Fernando Demarco, Mara Cristina Santos Felippe, Wilson Tadeu Felippe
Brazilian Dental Journal.2018; 29(5): 409. CrossRef
Regenerative Endodontic Treatment or Mineral Trioxide Aggregate Apical Plug in Teeth with Necrotic Pulps and Open Apices: A Systematic Review and Meta-analysis
Mahmoud Torabinejad, Ali Nosrat, Prashant Verma, Oyoyo Udochukwu
Journal of Endodontics.2017; 43(11): 1806. CrossRef
Revascularization in Immature Permanent Teeth with Necrotic Pulp and Apical Pathology: Case Series
López Carmen, Mendoza Asunción, Solano Beatriz, Yáñez-Vico Rosa
Case Reports in Dentistry.2017; 2017: 1. CrossRef

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Apexogenesis and revascularization treatment procedures for two traumatized immature permanent maxillary incisors: a case report: Maryam Forghani, Iman Parisay, Amir Maghsoudlou; Restor Dent Endod 2013;38(3):178-181. Published online August 23, 2013; DOI: https://doi.org/10.5395/rde.2013.38.3.178

Abstract PDF PubReader ePub

Traumatic injuries to an immature permanent tooth may result in cessation of dentin deposition and root maturation. Endodontic treatment is often complicated in premature tooth with an uncertain prognosis. This article describes successful treatment of two traumatized maxillary central incisors with complicated crown fracture three months after trauma. The radiographic examination showed immature roots in maxillary central incisors of a 9-year-old boy with a radiolucent lesion adjacent to the right central incisor. Apexogenesis was performed for the left central incisor and revascularization treatment was considered for the right one. In 18-month clinical and radiographic follow-up both teeth were asymptomatic, roots continued to develop, and periapical radiolucency of the right central incisor healed. Considering the root development of these contralateral teeth it can be concluded that revascularization is an appropriate treatment method in immature necrotic teeth.

Citations

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Mineral Trioxide Aggregate Apexogenesis: A Systematic Review
Basmah M. Ageel, Omar A. El Meligy, Sarah M. A. Quqandi
Journal of Pharmacy and Bioallied Sciences.2023; 15(Suppl 1): S11. CrossRef
Advances in Research on Stem Cell-Based Pulp Regeneration
Hua-Nien Lee, Cheng Liang, Li Liao, Wei-Dong Tian
Tissue Engineering and Regenerative Medicine.2021; 18(6): 931. CrossRef
Different Approaches to the Regeneration of Dental Tissues in Regenerative Endodontics
Anna M. Krupińska, Katarzyna Skośkiewicz-Malinowska, Tomasz Staniowski
Applied Sciences.2021; 11(4): 1699. CrossRef
A comparison of MTA and Biodentine as medicaments for pulpotomy in traumatized anterior immature permanent teeth: A randomized clinical trial
Gihan Mohamed Abuelniel, Monty Singh Duggal, Nihal Kabel
Dental Traumatology.2020; 36(4): 400. CrossRef
Bone, Periodontal and Dental Pulp Regeneration in Dentistry: A Systematic Scoping Review
Luiz Alexandre Chisini, Marcus Cristian Muniz Conde, Guillermo Grazioli, Alissa Schmidt San Martin, Rodrigo Varella de Carvalho, Letícia Regina Morello Sartori, Flávio Fernando Demarco
Brazilian Dental Journal.2019; 30(2): 77. CrossRef
Regenerative in endodontics: how, when and where
AL Ahmar Rima, Bassam Sanaa, Salloum Sarah, El Husseini Hassan, AL Ahmar Rima
Journal of Dental Health, Oral Disorders & Therapy.2018; 9(6): 531. CrossRef
Influence of Apical Diameter on the Outcome of Regenerative Endodontic Treatment in Teeth with Pulp Necrosis: A Review
Yanjun Fang, Xinhuan Wang, Jingjing Zhu, Chaonan Su, Ying Yang, Liuyan Meng
Journal of Endodontics.2018; 44(3): 414. CrossRef
Alternative to Avoid Tooth Discoloration after Regenerative Endodontic Procedure: A Systematic Review
Luciane Geanini Pena dos Santos, Luiz Alexandre Chisini, Camila Guerner Springmann, Beatriz Dulcineia Mendes de Souza, Fernanda Geraldo Pappen, Flávio Fernando Demarco, Mara Cristina Santos Felippe, Wilson Tadeu Felippe
Brazilian Dental Journal.2018; 29(5): 409. CrossRef
Retreatment of failed regenerative endodontic of orthodontically treated immature permanent maxillary central incisor: a case report
Musaed Fahad Al-Tammami, Saad A. Al-Nazhan
Restorative Dentistry & Endodontics.2017; 42(1): 65. CrossRef
Pulp Revascularization: A Literature Review
Pollyana Rodrigues de Souza Araújo, Luciano Barreto Silva, Alexandrino Pereira dos Santos Neto, José Alcides Almeida de Arruda, Pâmella Recco Álvares, Ana Paula Veras Sobral, Severino Alves Júnior, Jair Carneiro Leão, Rodivan Braz da Silva, Gerhilde Callo
The Open Dentistry Journal.2017; 10(1): 48. CrossRef
Rejeneratif Pulpa Tedavilerine Genel Bir Bakış
Elif Sungurtekin Ekçi
Cumhuriyet Dental Journal.2017; 19(3): 238. CrossRef
Regenerative Endodontics
Kristina Feigin, Bonnie Shope
Journal of Veterinary Dentistry.2017; 34(3): 161. CrossRef
A scoping review of root canal revascularization: relevant aspects for clinical success and tissue formation
M. C. M. Conde, L. A. Chisini, R. Sarkis‐Onofre, H. S. Schuch, J. E. Nör, F. F. Demarco
International Endodontic Journal.2017; 50(9): 860. CrossRef
Histologic Findings of a Human Immature Revascularized/Regenerated Tooth with Symptomatic Irreversible Pulpitis
Chufang Peng, Yuming Zhao, Wenjun Wang, Yuan Yang, Man Qin, Lihong Ge
Journal of Endodontics.2017; 43(6): 905. CrossRef
Regenerative Endodontic Treatment or Mineral Trioxide Aggregate Apical Plug in Teeth with Necrotic Pulps and Open Apices: A Systematic Review and Meta-analysis
Mahmoud Torabinejad, Ali Nosrat, Prashant Verma, Oyoyo Udochukwu
Journal of Endodontics.2017; 43(11): 1806. CrossRef
A Review of Tooth Discoloration after Regenerative Endodontic Therapy
Bill Kahler, Giampiero Rossi-Fedele
Journal of Endodontics.2016; 42(4): 563. CrossRef
Regenerative Endodontic Therapy: A Data Analysis of Clinical Protocols
Evangelos G. Kontakiotis, Christos G. Filippatos, Giorgos N. Tzanetakis, Anastasia Agrafioti
Journal of Endodontics.2015; 41(2): 146. CrossRef
Is revascularization of immature permanent teeth an effective and reproducible technique?
Yu‐Po Chen, Maria del Mar Jovani‐Sancho, Chirag C. Sheth
Dental Traumatology.2015; 31(6): 429. CrossRef
A review of the regenerative endodontic treatment procedure
Bin-Na Lee, Jong-Wook Moon, Hoon-Sang Chang, In-Nam Hwang, Won-Mann Oh, Yun-Chan Hwang
Restorative Dentistry & Endodontics.2015; 40(3): 179. CrossRef
Mineral trioxide aggregate upregulates odonto/osteogenic capacity of bone marrow stromal cells from craniofacial bones via JNK and ERK MAPK signalling pathways
Y. Wang, J. Li, W. Song, J. Yu
Cell Proliferation.2014; 47(3): 241. CrossRef
The use of platelet rich plasma in the treatment of immature tooth with periapical lesion: a case report
Günseli Güven Polat, Ceren Yıldırım, Özlem Martı Akgün, Ceyhan Altun, Didem Dinçer, Cansel Köse Özkan
Restorative Dentistry & Endodontics.2014; 39(3): 230. CrossRef
Levels of Evidence for the Outcome of Regenerative Endodontic Therapy
Evangelos G. Kontakiotis, Christos G. Filippatos, Anastasia Agrafioti
Journal of Endodontics.2014; 40(8): 1045. CrossRef
Platelet rich fibrin - a novel acumen into regenerative endodontic therapy
Kavita Hotwani, Krishna Sharma
Restorative Dentistry & Endodontics.2014; 39(1): 1. CrossRef
Adult stem cell-based apexogenesis
Yao Li
World Journal of Methodology.2014; 4(2): 99. CrossRef

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Review Article

Pulp tissue regeneration and root formation of permanent teeth with pulpal/periapical deseases: Yeon-Jee Yoo, Seung-Ho Baek, Ho-Hyun Son; J Korean Acad Conserv Dent 2010;35(4):238-245. Published online July 31, 2010; DOI: https://doi.org/10.5395/JKACD.2010.35.4.238

Abstract PDF PubReader ePub

Numerous cases about additional growth of roots or pulp tissue regeneration by using various intracanal medicaments in immature permanent teeth with periapical or pulpal disease have been reported. The underlying mechanism has not been clearly delineated, but it has been widely accepted that undifferentiated mesenchymal cells and stem cells are involved. Moreover, the growth and deposition of osteoid or cementoid tissues have been observed in regenerated pulp and roots. This new and non-invasive treatment has brightened the future of endodontics, and enlarged the vision of regenerative root canal treatment with multi-potent stem cells and various tissue engineering techniques.

Citations

Citations to this article as recorded by

Platelet rich fibrin - a novel acumen into regenerative endodontic therapy
Kavita Hotwani, Krishna Sharma
Restorative Dentistry & Endodontics.2014; 39(1): 1. CrossRef

160 View
1 Download
1 Crossref

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