Restorative Dentistry & Endodontics

Research Articles

Critical evaluation of fracture strength testing for endodontically treated teeth: a finite element analysis study: Emel Uzunoglu-Özyürek, Selen Küçükkaya Eren, Oğuz Eraslan, Sema Belli; Restor Dent Endod 2019;44(2):e15. Published online April 18, 2019; DOI: https://doi.org/10.5395/rde.2019.44.e15

Objectives

The aim of this study was to investigate whether the diameter and direction of the plunger and simulation of the periodontal ligament (PDL) affected the stress distribution in endodontically treated premolars.

Methods

A fracture strength test was simulated via finite element analysis. A base model was set up, and the following parameters were modified: plunger diameter (3 mm vs. 6 mm), plunger direction (vertical vs. 135° angular to the central fossa), and PDL simulation. The analysis was conducted using the CosmosWorks structural analysis program, and the results are presented in terms of von Mises stresses.

Results

The smaller plunger increased the stresses at the contact area of the crown, but the plunger diameter had no effect on the stress distribution within the root. An angular plunger direction increased stresses within the root, as well as at the buccal cusp of the crown, compared with the vertical direction. Simulation of the PDL caused higher stress accumulation, especially in the cervical region of the root.

Conclusions

The plunger diameter had no effect on the stress distribution in the roots, whereas the plunger direction and PDL simulation did affect the stress distribution. More stringent standards can be established by taking such parameters into account when performing fracture testing in future studies.

Citations

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Assessment of Stress Distribution with 3 Taper Design Preparation of Root Canal Using Finite Element Analysis
Tejasree Rathod, G. Durgabhavani, Pudu Tirupathi, Nusrath Parveen, Yelloji Paramesh, Prabhakar Dharavattu
Journal of Pharmacy and Bioallied Sciences.2024; 16(Suppl 1): S112. CrossRef
The impact of the filling technique with two sealers in bulk or associated with gutta-percha on the fatigue behavior and failure patterns of endodontically treated teeth
Isabella Marian Lena, Luiza Colpo Chiaratti, Rafaela Oliveira Pilecco, Renan Vaz Machry, João Paulo Mendes Tribst, Cornelis Johannes Kleverlaan, Gabriel Kalil Rocha Pereira, Renata Dornelles Morgental
PeerJ.2024; 12: e18221. CrossRef
Stronger than Ever: Multifilament Fiberglass Posts Boost Maxillary Premolar Fracture Resistance
Naji Kharouf, Eugenio Pedullà, Gianluca Plotino, Hamdi Jmal, Mohammed-El-Habib Alloui, Philippine Simonis, Patrice Laquerriere, Valentina Macaluso, Dina Abdellatif, Raphaël Richert, Youssef Haikel, Davide Mancino
Journal of Clinical Medicine.2023; 12(8): 2975. CrossRef
Neural network approach to evaluate the physical properties of dentin
Mohammad Ali Saghiri, Ali Mohammad Saghiri, Elham Samadi, Devyani Nath, Julia Vakhnovetsky, Steven M. Morgano
Odontology.2023; 111(1): 68. CrossRef
Modelling and evaluating periodontal ligament mechanical behaviour and properties: A scoping review of current approaches and limitations
Enaiyat Ghani Ovy, Dan L. Romanyk, Carlos Flores Mir, Lindsey Westover
Orthodontics & Craniofacial Research.2022; 25(2): 199. CrossRef
FEAr no more! Finite element analysis in orthodontics
Shilpa Chawla, Shailesh Deshmukh
Journal of the International Clinical Dental Research Organization.2022; 14(1): 6. CrossRef
Influence of Methodological Variables on Fracture Strength Tests Results of Premolars with Different Number of Residual Walls. A Systematic Review with Meta-Analysis
Carlo Gaeta, Crystal Marruganti, Emanuele Mignosa, Giovanni Franciosi, Edoardo Ferrari, Simone Grandini
Dentistry Journal.2021; 9(12): 146. CrossRef

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White mineral trioxide aggregate mixed with calcium chloride dihydrate: chemical analysis and biological properties: Hany Mohamed Aly Ahmed, Norhayati Luddin, Thirumulu Ponnuraj Kannan, Khairani Idah Mokhtar, Azlina Ahmad; Restor Dent Endod 2017;42(3):176-187. Published online April 17, 2017; DOI: https://doi.org/10.5395/rde.2017.42.3.176

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Objectives

This study aimed to evaluate the chemical and biological properties of fast-set white mineral trioxide aggregate (FS WMTA), which was WMTA combined with calcium chloride dihydrate (CaCl₂·2H₂O), compared to that of WMTA.

Materials and Methods

Surface morphology, elemental, and phase analysis were examined using scanning electron microscope (SEM), energy dispersive X-ray microanalysis (EDX), and X-ray diffraction (XRD), respectively. The cytotoxicity and cell attachment properties were evaluated on human periodontal ligament fibroblasts (HPLFs) using methyl-thiazol-diphenyltetrazolium (MTT) assay and under SEM after 24 and 72 hours, respectively.

Results

Results showed that the addition of CaCl₂·2H₂O to WMTA affected the surface morphology and chemical composition. Although FS WMTA exhibited a non-cytotoxic profile, the cell viability values of this combination were lesser than WMTA, and the difference was significant in 7 out of 10 concentrations at the 2 time intervals (p < 0.05). HPLFs adhered over the surface of WMTA and at the interface, after 24 hours of incubation. After 72 hours, there were increased numbers of HPLFs with prominent cytoplasmic processes. Similar findings were observed with FS WMTA, but the cells were not as confluent as with WMTA.

Conclusions

The addition of CaCl₂·2H₂O to WMTA affected its chemical properties. The favorable biological profile of FS WMTA towards HPLFs may have a potential impact on its clinical application for repair of perforation defects.

Citations

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The effect of three additives on properties of mineral trioxide aggregate cements: a systematic review and meta-analysis of in vitro studies
Behnam Bolhari, Faranak Noori, Hadi Assadian, Amir Raee, Sholeh Ghabraei, Ahmad-Reza Shamshiri, Artak Heboyan
BMC Oral Health.2024;[Epub] CrossRef
Evaluation of sorption and solubility of materials based on calcium aluminate
Renata Josipovic, Violeta Petrovic, Marijana Popovic-Bajic, Irena Kuzmanovic-Radman, Mirjana Umicevic-Davidovic, Aleksandra Djeri, Slavoljub Zivkovic
Stomatoloski glasnik Srbije.2023; 70(1): 26. CrossRef
Chitosan-Based Accelerated Portland Cement Promotes Dentinogenic/Osteogenic Differentiation and Mineralization Activity of SHED
Hasan Subhi, Adam Husein, Dasmawati Mohamad, Nik Rozainah Nik Abdul Ghani, Asma-Abdullah Nurul
Polymers.2021; 13(19): 3358. CrossRef
Chemical modification of MTA and CEM cement to decrease setting time and improve bioactivity properties by adding alkaline salts
Faeze Jamali Zavare, Hanieh Nojehdehian, Maryam Moezizadeh, Mehdi Daneshpooya
Journal of Dental Research, Dental Clinics, Dental Prospects.2020; 14(1): 1. CrossRef
Biological effects of acid-eroded MTA Repair HP and ProRoot MTA on human periodontal ligament stem cells
Mar Collado-González, Sergio López-García, David García-Bernal, Ricardo E. Oñate-Sánchez, Christopher J. Tomás-Catalá, Jose M. Moraleda, Adrián Lozano, Leopoldo Forner, Francisco J. Rodríguez-Lozano
Clinical Oral Investigations.2019; 23(10): 3915. CrossRef
Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer
Sergio López-García, Miguel R. Pecci-Lloret, Julia Guerrero-Gironés, María P. Pecci-Lloret, Adrián Lozano, Carmen Llena, Francisco Javier Rodríguez-Lozano, Leopoldo Forner
Materials.2019; 12(19): 3087. CrossRef
Evaluation of changes in ion release and biological properties of NeoMTA‐Plus and Endocem‐MTA exposed to an acidic environment
F. J. Rodríguez‐Lozano, M. Collado‐González, S. López‐García, D. García‐Bernal, J. M. Moraleda, A. Lozano, L. Forner, L. Murcia, R. E. Oñate‐Sánchez
International Endodontic Journal.2019; 52(8): 1196. CrossRef

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Effects of proanthocyanidin, a crosslinking agent, on physical and biological properties of collagen hydrogel scaffold: Yoorina Choi, Hee-Jin Kim, Kyung-San Min; Restor Dent Endod 2016;41(4):296-303. Published online October 4, 2016; DOI: https://doi.org/10.5395/rde.2016.41.4.296

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Objectives

The purpose of the present study was to evaluate the effects of proanthocyanidin (PAC), a crosslinking agent, on the physical properties of a collagen hydrogel and the behavior of human periodontal ligament cells (hPDLCs) cultured in the scaffold.

Materials and Methods

Viability of hPDLCs treated with PAC was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The physical properties of PAC treated collagen hydrogel scaffold were evaluated by the measurement of setting time, surface roughness, and differential scanning calorimetry (DSC). The behavior of the hPDLCs in the collagen scaffold was evaluated by cell morphology observation and cell numbers counting.

Results

The setting time of the collagen scaffold was shortened in the presence of PAC (p < 0.05). The surface roughness of the PAC-treated collagen was higher compared to the untreated control group (p < 0.05). The thermogram of the crosslinked collagen exhibited a higher endothermic peak compared to the uncrosslinked one. Cells in the PAC-treated collagen were observed to attach in closer proximity to one another with more cytoplasmic extensions compared to cells in the untreated control group. The number of cells cultured in the PAC-treated collagen scaffolds was significantly increased compared to the untreated control (p < 0.05).

Conclusions

Our results showed that PAC enhanced the physical properties of the collagen scaffold. Furthermore, the proliferation of hPDLCs cultured in the collagen scaffold crosslinked with PAC was facilitated. Conclusively, the application of PAC to the collagen scaffold may be beneficial for engineering-based periodontal ligament regeneration in delayed replantation.

Citations

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A highly biocompatible CE-crosslinked collagen implant with exceptional anti-calcification and collagen regeneration capabilities for aging skin rejuvenation
Qi Wang, Huiyu Yan, Linyan Yao, Wenhua Li, Jianxi Xiao
Journal of Materials Chemistry B.2024; 12(18): 4467. CrossRef
Dexamethasone release from hyaluronic acid microparticle and proanthocyanidin-gelatin hydrogel in sciatic tissue regeneration
Kazem Javanmardi, Hamideh Shahbazi, Ava Soltani Hekmat, Mehdi Khanmohammadi, Arash Goodarzi
Journal of Materials Science: Materials in Medicine.2024;[Epub] CrossRef
New Materials Based on Collagen and Taxifolin Derivatives: Production and Properties
Yu. V. Shatalin, M. I. Kobyakova, V. S. Shubina
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Yu. V. Shatalin, M. I. Kobyakova, V. S. Shubina
Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology.2023; 17(S1): S85. CrossRef
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Wedja Timóteo Vieira, Meuris Gurgel Carlos da Silva, Laura de Oliveira Nascimento, Melissa Gurgel Adeodato Vieira
International Journal of Biological Macromolecules.2023; 246: 125558. CrossRef
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Michael B. Scott, Amy K. Styring, James S. O. McCullagh
Pathogens.2022; 11(7): 770. CrossRef
Advances of Hydrogel Therapy in Periodontal Regeneration—A Materials Perspective Review
Maoxue Li, Jiaxi Lv, Yi Yang, Guoping Cheng, Shujuan Guo, Chengcheng Liu, Yi Ding
Gels.2022; 8(10): 624. CrossRef
Collagen stabilization by natural cross-linkers: A qualitative and quantitative FTIR study on ultra-thin dentin collagen model
Rong WANG, Tyler STANLEY, Xiaomei YAO, Hang LIU, Yong WANG
Dental Materials Journal.2022; 41(3): 440. CrossRef
Cross-Linking Agents for Electrospinning-Based Bone Tissue Engineering
Dong-Jin Lim
International Journal of Molecular Sciences.2022; 23(10): 5444. CrossRef
Dense lamellar scaffold, biomimetically inspired, for reverse cardiac remodeling: Effect of proanthocyanidins and glutaraldehyde
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Journal of Dispersion Science and Technology.2021; 42(2): 248. CrossRef
The effect of the cross-linker ratio used in gellan gum biomaterial synthesis on biomineralization
Serbülent TÜRK, Burak ÜNLÜ, Mahmut ÖZACAR
Bulletin of Biotechnology.2021; 2(2): 27. CrossRef
The recent advances in scaffolds for integrated periodontal regeneration
Hyun Nyun Woo, Young Joon Cho, Solaiman Tarafder, Chang H. Lee
Bioactive Materials.2021; 6(10): 3328. CrossRef
Plant based cross-linkers for tissue engineering applications
Abhishek Indurkar, Ashish Pandit, Ratnesh Jain, Prajakta Dandekar
Journal of Biomaterials Applications.2021; 36(1): 76. CrossRef
Plant-based biomaterials in tissue engineering
Abhishek Indurkar, Ashish Pandit, Ratnesh Jain, Prajakta Dandekar
Bioprinting.2021; 21: e00127. CrossRef
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Huijuan Tang, Andrell Hosein, Monica Mattioli-Belmonte
Materials Science and Engineering: C.2021; 120: 111760. CrossRef
Adsorption of Gold Ions onto Sericin and Alginate Particles Chemically Crosslinked by Proanthocyanidins: a Complete Fixed-Bed Column Study
Nilza Tatiane das Graças Santos, Richard Landers, Meuris Gurgel Carlos da Silva, Melissa Gurgel Adeodato Vieira
Industrial & Engineering Chemistry Research.2020; 59(1): 318. CrossRef
Proanthocyanidin as a crosslinking agent for fibrin, collagen hydrogels and their composites with decellularized Wharton’s-jelly-extract for tissue engineering applications
Elham Hasanzadeh, Narges Mahmoodi, Arefeh Basiri, Faezeh Esmaeili Ranjbar, Zahra Hassannejad, Somayeh Ebrahimi-Barough, Mahmoud Azami, Jafar Ai, Vafa Rahimi-Movaghar
Journal of Bioactive and Compatible Polymers.2020; 35(6): 554. CrossRef
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Natural biopolymer‐based hydrogels for use in food and agriculture
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Novel Biomedical Applications of Crosslinked Collagen
Lisha Gu, Tiantian Shan, Yu-xuan Ma, Franklin R. Tay, Lina Niu
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Stomatologiya.2018; 97(6): 78. CrossRef

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Basic Researchs

The effect of tumor necrosis factor (TNF)-α to induce matrix metalloproteinase (MMPs) from the human dental pulp, gingival, and periodontal ligament cells: Eun-Mi Rhim, Sang-Hyuk Park, Duck-Su Kim, Sun-Young Kim, Kyoung-Kyu Choi, Gi-Woon Choi; J Korean Acad Conserv Dent 2011;36(1):26-36. Published online January 31, 2011; DOI: https://doi.org/10.5395/JKACD.2011.36.1.26

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Objectives

In the present study, three kinds of tissues cells (pulp, gingiva, and periodontal ligament) were investigated if those cells express MMP and TIMP when they were stimulated with neuropeptides (substance P, CGRP) or proinflammatory cytokine, TNF-α.

Materials and Methods

The cells cultured from human dental pulp (PF), gingiva (GF) and periodontal ligament were (PDLF) stimulated with Mock, SP, TNF-α, and CGRP for 24 hrs and 48 hrs. for an RNase protection assay and Enzyme Linked Immunosorbent Assay.

Cells (PF, GF and PDLF) seeded in 100 mm culture dish were stimulated with SP (10^-5, 10^-8 M) or only with medium (Mock stimulation) for 4hrs and for 24 hrs for RNase Protection Assay, and they were stimulated with CGRP (10^-5 M) and TNF-α (2 ng/mL) for 24 hrs and with various concentraion of TNF-α (2, 10, and 100 ng/mL) for Rnase Protection Assay with a human MMP-1 probe set including MMP 1, 2, 8, 7, 8, 9, 12, and TIMP 2, 3.

In addition, cells (PF, GF and PDLF) were stimulated with Mock and various concentraion of TNF-α (2, 10, and 100 ng/mL) for 24 hrs and with TNF-α (10 ng/mL) for 48 hrs, and the supernatents from the cells were collected for Enzyme Linked Immunosorbent Assay (ELISA) for MMP-1 and MMP-13.

Results

The expression of MMPs in PF, GF, PDLF after stimulation with SP and CGRP were not changed compared with Mock stimulation for 4 hrs and 24 hrs. The expression of MMP-1, -12, -13 24 hrs after stimulation with TNF-α were upregulated, however the expression of TIMP-3 in PF, GF, PDLF after stimulation with TNF-α were downregulated. TNF-α (2 ng/mL, 10 ng/mL, 100 ng/mL) increased MMP-1 and MMP-12 expression in PF dose dependently for 24 hrs.

Conclusions

TNF-α in the area of inflammation may play an important role in regulating the remodeling of dentin, cementum, and alveolar bone.

Citations

Citations to this article as recorded by

Anti‐Inflammatory Effects of Melatonin and 5‐Methoxytryptophol on Lipopolysaccharide‐Induced Acute Pulpitis in Rats
Fatma Kermeoğlu, Umut Aksoy, Abdullah Sebai, Gökçe Savtekin, Hanife Özkayalar, Serkan Sayıner, Ahmet Özer Şehirli, Shuai CHEN
BioMed Research International.2021;[Epub] CrossRef
Cross-Talk between Ciliary Epithelium and Trabecular Meshwork Cells In-Vitro: A New Insight into Glaucoma
Natalie Lerner, Elie Beit-Yannai, Wayne Iwan Lee Davies
PLoS ONE.2014; 9(11): e112259. CrossRef

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Biocompatibility of bioaggregate cement on human pulp and periodontal ligament (PDL) derived cells: Choo-Ryung Chung, Euiseong Kim, Su-Jung Shin; J Korean Acad Conserv Dent 2010;35(6):473-478. Published online November 30, 2010; DOI: https://doi.org/10.5395/JKACD.2010.35.6.473

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Objectives

This study was performed to investigate the biocompatibility of newly introduced Bioaggregate on human pulp and PDL cells.

Materials and Methods

Cells were collected from human pulp and PDL tissue of extracted premolars. Cell culture plate was coated either with Bioaggregate or white MTA, then the same number of cells were poured to cell culture dishes. Cell attachment and growth was examined under a phase microscope after 1,3 and 7 days of seeding. Cell viability was measured and the data was analyzed using Student t-test and one way ANOVA.

Results

Both types of cells used in this study were well attached and grew healthy on Bioaggregate and MTA coated culture dishes. No cell inhibition zone was observed in Bioaggregate group. There was no statistical difference of viable cells between bioaggreagte and MTA groups.

Conclusions

Bioaggregate appeared to be biocompatible compared with white MTA on human pulp and PDL cells.

Citations

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Evaluation of bioactivity, biocompatibility, and antibacterial properties of tricalcium silicate bone cement modified with wollastonite/ fluorapatite glass and glass-ceramic
H.K. Abd El-Hamid, A.M. Fayad, R.L. Elwan
Ceramics International.2024; 50(14): 25322. CrossRef
Influence of insulin on the healing of exposed dental pulp after pulp capping: An experimental study in a dog model
Mokhtar A. Al‐Anesi, Ashraf M. Abu‐Seida, Salma H. El Ashry, Abeer H. Mahran, Ehab S. Abd‐Elhamid
Special Care in Dentistry.2021; 41(1): 49. CrossRef
ROOT END FILLING MATERIALS – A REVIEW
Bynagari Chandra Shekar, Veerendra Uppin, Madhu Pujar
GLOBAL JOURNAL FOR RESEARCH ANALYSIS.2021; : 5. CrossRef
Effects of two fast-setting calcium-silicate cements on cell viability and angiogenic factor release in human pulp-derived cells
Chooryung J. Chung, Euiseong Kim, Minju Song, Jeong-Won Park, Su-Jung Shin
Odontology.2016; 104(2): 143. CrossRef
Cytotoxicity and physical properties of tricalcium silicate-based endodontic materials
Young-Eun Jang, Bin-Na Lee, Jeong-Tae Koh, Yeong-Joon Park, Nam-Eok Joo, Hoon-Sang Chang, In-Nam Hwang, Won-Mann Oh, Yun-Chan Hwang
Restorative Dentistry & Endodontics.2014; 39(2): 89. CrossRef
Biocompatibility of root-end filling materials: recent update
Payal Saxena, Saurabh Kumar Gupta, Vilas Newaskar
Restorative Dentistry & Endodontics.2013; 38(3): 119. CrossRef

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The evaluation of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure: Jin-Ho Chung, Jin Kim, Seong-Ho Choi, Eui-Seong Kim, Jiyong Park, Seung-Jong Lee; J Korean Acad Conserv Dent 2010;35(4):285-294. Published online July 31, 2010; DOI: https://doi.org/10.5395/JKACD.2010.35.4.285

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The purpose of this study was to evaluate the viability of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure by means of MTT assay, WST-1 assay. 12 teeth of Sprague-Dawley white female rats of 4 week-old were used for each group.

Both side of the first and second maxillary molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were group 1 (Immediate extraction), group 2 (Slow freezing under pressure of 3 MPa), group 3 (Slow freezing under pressure of 2 MPa), group 4 (Slow freezing under no additional pressure), group 5 (Rapid freezing in liquid nitrogen under pressure of 2 MPa), group 6 (Rapid freezing in liquid nitrogen under no additional pressure), group 7 (low-temperature preservation at 0℃ under pressure of 2 MPa), group 8 (low-temperature preservation at 0℃ under no additional pressure), group 9 (low-temperature preservation at -5℃ under pressure of 90 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in 37℃ water bath, then MTT assay, WST-1 assay were processed. One way ANOVA and Tukey HSD method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization.

In both MTT and WST-1 assay, group 7 (0℃/2 MPa) showed higher viability of periodontal ligament cells than other group (2-6, 8) and this was statistically significant (p < 0.05), but showed lower viability than group 1, immediate extraction group (no statistical significance).

By the results of this study, low-temperature preservation at 0℃ under pressure of 2 MPa suggest the possibility for long term preservation of teeth.

Citations

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Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0℃/2 MPa Condition up to One Week: In Vivo MTT Method
Sun Mi Jang, Sin-Yeon Cho, Eui-Seong Kim, Il-Young Jung, Seung Jong Lee
Journal of Korean Dental Science.2016; 9(1): 1. CrossRef

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Original Articles

The comparison of gene expression from human dental pulp cells and periodontal ligament cells: Hyoun So, Sang-Hyuk Park, Gi-Woon Choi; J Korean Acad Conserv Dent 2009;34(5):430-441. Published online September 30, 2009; DOI: https://doi.org/10.5395/JKACD.2009.34.5.430

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The purpose of this study was to characterize functional distinction between human dental pulp cells(PC) and periodontal ligament cells(PDLC) using cDNA microarray assay and to confirm the results of the microarray assay using RT-PCR. 3 genes out of 51 genes which were found to be more expressed(>2 fold) in PC were selected, and 3 genes out of 19 genes which were found to be more expressed(>2 fold) in PDLC were selected for RT-PCR as well.

According to this study, the results were as follows:

1. From the microarray assay, 51 genes were more expressed (2 fold) from PC than PDLC.

2. RT-PCR confirmed that ITGA4 and TGF β2 were more expressed in PC than in PDLC.

3. From the microarray assay, 19 genes were more expressed (2 fold) from PDLC than PC.

4. RT-PCR confirmed that LUM, WISP1, and MMP1 were more expressed in PDLC than in PC.

From the present study, different expression of the genes between the PC and PDLC were characterized to show the genes which play an important role in dentinogenesis were more expressed from PC than PDLC, while the genes which were related with collagen synthesis were more expressed from PDLC than PC.

Citations

Citations to this article as recorded by

Gene expression profiling in human dental pulp cells treated with mineral trioxide aggregate
Yong-Beom Kim, Won-Jun Shon, WooCheol Lee, Kee-Yeon Kum, Seung-Ho Baek, Kwang-Shik Bae
Journal of Korean Academy of Conservative Dentistry.2010; 35(3): 152. CrossRef

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THE EFFICACY OF PROGRAMMED CRYO-PRESERVATION UNDER PRESSURE IN RAT PERIODONTAL LIGAMENT CELLS: Young-Eun Lee, Eui-Seong Kim, Jin Kim, Seung-Hoon Han, Seung-Jong Lee; J Korean Acad Conserv Dent 2009;34(4):356-363. Published online January 14, 2009; DOI: https://doi.org/10.5395/JKACD.2009.34.4.356

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Abstract

The purpose of this study was to evaluate the viability of periodontal ligament cells in rat teeth using slow cryo-preservation method under pressure by means of MTT assay and WST-1 assay. Eighteen teeth of Sprague-Dawley white female rats of 4 week-old were used for each group.

Both sides of the first and second maxillary molars were extracted as atraumatically as possible under Tiletamine anesthesia. The experimental groups were group 1 (Immediate control), group 2 (Cold preservation at 4°C for 1 week), group 3 (Slow freezing), group 4 (Slow freezing under pressure of 3 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in 37°C water bath, then MTT assay and WST-1 assay were processed. One way ANOVA and Tukey method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization.

In both MTT and WST-1 assay, group 4 showed significantly higher viability of periodontal ligament cells than group 2 and 3 (p < 0.05), but showed lower viability than immediate control group.

By the results of this study, slow cryo-preservation method under pressure suggests the possibility for long term cryo-preservation of the teeth.

Citations

Citations to this article as recorded by

Effects of Slow Programmable Cryopreservation on Preserving Viability of the Cultured Periodontal Ligament Cells from Human Impacted Third Molar
Jin-Woo Kim, Tae-Yi Kim, Ye-mi Kim, Eun-Kyoung Pang, Sun-Jong Kim
Journal of Korean Dental Science.2015; 8(2): 57. CrossRef
The evaluation of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure
Jin-Ho Chung, Jin Kim, Seong-Ho Choi, Eui-Seong Kim, Jiyong Park, Seung-Jong Lee
Journal of Korean Academy of Conservative Dentistry.2010; 35(4): 285. CrossRef
Comparison of viability of oral epithelial cells stored by different freezing methods
Do-Young Baek, Seung-Jong Lee, Han-Sung Jung, EuiSeong Kim
Journal of Korean Academy of Conservative Dentistry.2009; 34(6): 491. CrossRef

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Evaluation of the viability of periodontal ligament cell in rat teeth using slow cryopreservation method with magnetic field: Hyun-Jung Ahn, Eui-Seong Kim, Jin Kim, Duck-Won Kim, Ki-Yeol Kim, Chan-Young Lee, Seung-Jong Lee; J Korean Acad Conserv Dent 2008;33(4):332-340. Published online July 31, 2008; DOI: https://doi.org/10.5395/JKACD.2008.33.4.332

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The purpose of this study was to evaluate the viability of periodontal ligament cell in rat teeth using slow cryopreservation method with magnetic field through MTT assay and TUNEL test. For each group, 12 teeth of 4 weeks old white female Sprague-Dawley rat were used for MTT assay, and 6 teeth in TUNEL test. The Maxillary left and right, first and second molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were group1 (immediately extraction), group 2 (cold preservation at 4℃ for 1 week), group 3 (rapid cryopreservation in liquid nitrogen), group 4 (slow cryopreservation with magnetic field of 1 G), and group 5 (slow cryopreservation). F medium was used as preservation medium and 10% DMSO as cryoprotectant. After preservation and thawing, the MTT assay and TUNEL test were processed. One way ANOVA and Scheffe method were performed at the 95% level of confidence. The value of optical density obtained after MTT analysis was divided by the value of eosin staining for tissue volume standardization. In both MTT assay and TUNEL test, it had showed no significant difference among group 3, 4, and 5. And group 3 had showed higher viability of periodontal ligament cell than group 2.

From this study, slow cryopreservation method with magnetic field can be used as one of cryopreservation methods.

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The evaluation of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure
Jin-Ho Chung, Jin Kim, Seong-Ho Choi, Eui-Seong Kim, Jiyong Park, Seung-Jong Lee
Journal of Korean Academy of Conservative Dentistry.2010; 35(4): 285. CrossRef
Comparison of viability of oral epithelial cells stored by different freezing methods
Do-Young Baek, Seung-Jong Lee, Han-Sung Jung, EuiSeong Kim
Journal of Korean Academy of Conservative Dentistry.2009; 34(6): 491. CrossRef
The efficacy of programmed cryo-preservation under pressure in rat periodontal ligament cells
Young-Eun Lee, Eui-Seong Kim, Jin Kim, Seung-Hoon Han, Seung-Jong Lee
Journal of Korean Academy of Conservative Dentistry.2009; 34(4): 356. CrossRef

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Evaluation of the radiopacity and cytotoxicity of resinous root canal sealers: Chang-Kyu Kim, Hyun-Wook Ryu, Hoon-Sang Chang, Byung-Do Lee, Kyung-San Min, Chan-Ui Hong; J Korean Acad Conserv Dent 2007;32(5):419-425. Published online September 30, 2007; DOI: https://doi.org/10.5395/JKACD.2007.32.5.419

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The aim of this study was to evaluate the radiopacity and cytotoxicity of three resin-based (AH 26, EZ fill and AD Seal), a zinc oxide-eugenol-based (ZOB Seal), and a calcium hydroxide-based (Sealapex) root canal sealers. Specimens, 10 mm in diameter and 1 mm in thickness, were radiographed simultaneously with an aluminum step wedge using occlusal films, according to ISO 6876/2001 standards. Radiographs were digitized, and the radiopacity of sealers was compared to the different thicknesses of the aluminum step wedge, using the Scion image software. Using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the cytotoxicity of each material was determined in immortalized human periodontal ligament (IPDL) cells.

The results demonstrated that EZ fill was the most radiopaque sealer, while Sealapex was the least radiopaque (p < 0.05). AH 26, AD Seal and ZOB Seal presented intermediate radiopacity values. All the materials evaluated, except for Sealapex, presented the minimum radiopacity required by ISO standards. The cell viabilities of resin-based root canal sealers were statistically higher than that of other type of root canal sealers through the all experimental time. Further, EZ fill showed statistically lower cell viability in 24 and 48 hours compared to AD Seal and in 72 hours compared to all other resin-based root canal sealers. However, there was no correlation between the radiopacity and cytotoxicity of three resin-based root canals sealers (p > 0.05).

These results indicate that resin-based root canal sealer is more biocompatible and has advantage in terms of radiopacity.

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A Comparative Evaluation of Two Commonly Used GP Solvents on Different Epoxy Resin-based Sealers: An In Vitro Study
Sakshi Tyagi, Ekta Choudhary, Rajat Chauhan, Ashish Choudhary
International Journal of Clinical Pediatric Dentistry.2020; 13(1): 35. CrossRef
Evaluation of softening ability of Xylene & Endosolv-R on three different epoxy resin based sealers within 1 to 2 minutes - anin vitrostudy
Pratima Ramakrishna Shenoi, Gautam Pyarelal Badole, Rajiv Tarachand Khode
Restorative Dentistry & Endodontics.2014; 39(1): 17. CrossRef
A comparative evaluation of cytotoxicity of root canal sealers: anin vitrostudy
Gautam Pyarelal Badole, Manjusha Madhukar Warhadpande, Ganesh Kothiramji Meshram, Rakesh Namdeoraoji Bahadure, Shubha Gopal Tawani, Gopal Tawani, Shital Gautam Badole
Restorative Dentistry & Endodontics.2013; 38(4): 204. CrossRef
Evaluation of radiopacity and discriminability of various fiber reinforced composite posts
Eun-Hye Lee, Hang-Moon Choi, Se-Hee Park, Jin-Woo Kim, Kyung-Mo Cho
Journal of Korean Academy of Conservative Dentistry.2010; 35(3): 188. CrossRef
A comparative study on radiopacity of root canal sealers
Tae-Min Kim, Seo-Kyoung Kim, In-Nam Hwang, Yun-Chan Hwang, Byung-Cheol Kang, Suk-Ja Yoon, Jae-Seo Lee, Won-Mann Oh
Journal of Korean Academy of Conservative Dentistry.2009; 34(1): 61. CrossRef
A comparative study on radiopacity of canal filling and retrograde root-end filling materials
Yong-Sang Kim, Seo-Kyong Kim, Yun-Chan Hwang, In-Nam Hwang, Won-Mann Oh
Journal of Korean Academy of Conservative Dentistry.2008; 33(2): 107. CrossRef

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Evaluation of periodontal ligament cell viability in rat teeth according to various extra-oral dry storage times using MTT assay: In-Soo Jeon, Eui-Seong Kim, Jin Kim, Seung-Jong Lee; J Korean Acad Conserv Dent 2006;31(5):398-408. Published online September 30, 2006; DOI: https://doi.org/10.5395/JKACD.2006.31.5.398

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The purpose of this study was to verify the usefulness of MTT analysis as a tool of measurement of the periodontal ligament cell viability from the extracted rat molar.

A total of 80 Sprague-Dawley white female rat of 4 week-old with a body weight of 100 grams were used. The maxillary left and right, first and second molars were extracted under Ketamine anesthesia. Twenty-four teeth of each group (divided as five groups depending upon the time-lapse after extraction such as immediate, 10, 20, 40 and 60 minutes) were immersed in 200 µl of MTT solution (0.5 mg/ml) and processed for optical density measurements . Another 10 teeth of each group were treated as same as above and sectioned at 10 µm for microscopic examination.

All measurements values were divided by the value of hematoxylin-eosin staining which represented the volume of each corresponding samples. Immediate and 10 minute groups showed highest MTT values followed by 20, 40, and 60 minutes consecutively. Statistical significance (p < 0.05) existed between all groups except in immediate versus 10 minute groups and 40 versus 60 minutes. Histological findings also showed similar findings with MTT results in crystal shape and crystal numbers between the experimental groups.

These data indicate that in vivo MTT analysis may be of value for evaluation of the periodontal ligament cell viability without time- consuming cell culturing processes.

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Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0℃/2 MPa Condition up to One Week: In Vivo MTT Method
Sun Mi Jang, Sin-Yeon Cho, Eui-Seong Kim, Il-Young Jung, Seung Jong Lee
Journal of Korean Dental Science.2016; 9(1): 1. CrossRef

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Evaluation of periodontal ligament cell viability in rat teeth after frozen preservation using in-vivo MTT assay: Jae-Wook Kim, Eui-Sung Kim, Jin Kim, Seung-Jong Lee; J Korean Acad Conserv Dent 2006;31(3):192-202. Published online May 31, 2006; DOI: https://doi.org/10.5395/JKACD.2006.31.3.192

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The purpose of this study was to examine the viability of PDL cells in rat molars by using in vivo MTT assay, which was used to compare fast cryopreservation group by liquid nitrogen (-196℃) with 4℃ cold preservation group.

A total of 74 Sprague-Dawley white female rats of 4 week-old with a body weight of 100 grams were used. The maxillary left and right, first and second molars were extracted as atraumatically as possible under ketamine anesthesia.

Ten teeth of each group were divided as six experimental groups depending upon the preservation. Cryopreservation groups were Group 1 (5% DMSO 6% HES in F medium), Group 2 (10% DMSO in F medium), Group 3 (5% DMSO 6% HES in Viaspan®), Group 4 (10% DMSO in Viaspan®) which were cryopreserved for 1 week and cold preservation groups were Group 5 (F medium), Group 6 (Viaspan®) at 4℃ for 1 week. Immediate extraction group was used as a control. After preservation and thawing, the in vivo MTT assay was processed. Two way ANOVA and Duncan's Multiple Range Test was performed at the 95% level of confidence. Another 2 teeth of each group were treated as the same manner and frozen sections 10 µm thick for microscopic observation.

The value of optical density obtained after in vivo MTT analysis was divided by the value of eosin staining for tissue volume standardization. Group 1, 2 had significantly higher optical density than Group 3 and 4 which had the lowest OD value. Group 6 had higher OD value than in Group 5 (P < 0.05). Histological findings of periodontal ligament cell, after being stained with MTT solution were consistent with the in vivo MTT assay results.

In this study, the groups which were frozen with DMSO as a cryoprotectant and the groups with F medium showed the best results.

Citations

Citations to this article as recorded by

Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0℃/2 MPa Condition up to One Week: In Vivo MTT Method
Sun Mi Jang, Sin-Yeon Cho, Eui-Seong Kim, Il-Young Jung, Seung Jong Lee
Journal of Korean Dental Science.2016; 9(1): 1. CrossRef
Comparative study on survival rate of human gingival fibroblasts stored in different storage media
Hee Su Lee, You Sun Lim
Journal of Korean society of Dental Hygiene.2012; 12(4): 733. CrossRef
The evaluation of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure
Jin-Ho Chung, Jin Kim, Seong-Ho Choi, Eui-Seong Kim, Jiyong Park, Seung-Jong Lee
Journal of Korean Academy of Conservative Dentistry.2010; 35(4): 285. CrossRef
Comparison of viability of oral epithelial cells stored by different freezing methods
Do-Young Baek, Seung-Jong Lee, Han-Sung Jung, EuiSeong Kim
Journal of Korean Academy of Conservative Dentistry.2009; 34(6): 491. CrossRef
The efficacy of programmed cryo-preservation under pressure in rat periodontal ligament cells
Young-Eun Lee, Eui-Seong Kim, Jin Kim, Seung-Hoon Han, Seung-Jong Lee
Journal of Korean Academy of Conservative Dentistry.2009; 34(4): 356. CrossRef

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The effect of neuropeptides on secretion of Interleukin-8 (IL-8): Kyung-Jun Kim, Sang-Hyuk Park, Kyoung-Kyu Choi, Sang-Jin Park; J Korean Acad Conserv Dent 2006;31(3):153-160. Published online May 31, 2006; DOI: https://doi.org/10.5395/JKACD.2006.31.3.153

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We investigated the secretion of Interleukin-8 (IL-8) from ginviva and periodontal ligament stimulated with Substance P (SP) and Calcitonin Gene-related Peptide (CGRP). Gingiva (GF), periodontal ligament (PDLF) and pulp (PF) tissues were collected from extracted intact 3rd molars.

Cultured cells were stimulated with different concentrations of SP for 4 hrs, and stimulated with SP, CGRP and Tumor Necrosis Factor-α (TNF-α) for 8 hrs. Then RNase Protection Assay was carried out. ELISA was performed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using student t-test with significance of P < 0.05.

According to this study, the results were as follows:

IL-8 mRNA was detected in all type of cells studied (PF, GF and PDLF).

IL-8 mRNA expression was not increased after stimulating 4 hrs with SP (10^-5M) and SP (10^-8M) compared with Mock stimulation in all type of cells studied.

IL-8 mRNA expression was not increased after stimulating 8 hrs with SP (10^-4M) and CGRP (10^-6M) compared with Mock stimulation in all type of cells studied.

TNF-α(2 ng/ml) increased the expression of IL-8 mRNA in all kind of cells studied.

The secretion of IL-8 from GF was increased 8 hrs after the stimulation with CGRP (10^-6M) (p < 0.05).

The secretion of IL-8 from PDLF was increased 8 hrs after the stimulation with SP (10^-4M) (p < 0.05).

Calcitonin Gene-related Peptide (CGRP) increased Interleukin-8 (IL-8) which plays an important role in chemotaxis of neutrophil in Calcitonin Gene-related Peptide (CGRP) gingival tissue, whereas Substance P increased the secretion of IL-8 from periodontal ligament.

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