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The comparison of gene expression from human dental pulp cells and periodontal ligament cells
Hyoun So, Sang-Hyuk Park, Gi-Woon Choi
J Korean Acad Conserv Dent 2009;34(5):430-441.   Published online September 30, 2009
DOI: https://doi.org/10.5395/JKACD.2009.34.5.430
AbstractAbstract PDFPubReaderePub

The purpose of this study was to characterize functional distinction between human dental pulp cells(PC) and periodontal ligament cells(PDLC) using cDNA microarray assay and to confirm the results of the microarray assay using RT-PCR. 3 genes out of 51 genes which were found to be more expressed(>2 fold) in PC were selected, and 3 genes out of 19 genes which were found to be more expressed(>2 fold) in PDLC were selected for RT-PCR as well.

According to this study, the results were as follows:

1. From the microarray assay, 51 genes were more expressed (2 fold) from PC than PDLC.

2. RT-PCR confirmed that ITGA4 and TGF β2 were more expressed in PC than in PDLC.

3. From the microarray assay, 19 genes were more expressed (2 fold) from PDLC than PC.

4. RT-PCR confirmed that LUM, WISP1, and MMP1 were more expressed in PDLC than in PC.

From the present study, different expression of the genes between the PC and PDLC were characterized to show the genes which play an important role in dentinogenesis were more expressed from PC than PDLC, while the genes which were related with collagen synthesis were more expressed from PDLC than PC.

Citations

Citations to this article as recorded by  
  • Gene expression profiling in human dental pulp cells treated with mineral trioxide aggregate
    Yong-Beom Kim, Won-Jun Shon, WooCheol Lee, Kee-Yeon Kum, Seung-Ho Baek, Kwang-Shik Bae
    Journal of Korean Academy of Conservative Dentistry.2010; 35(3): 152.     CrossRef
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Selective detection of viable Enterococcus faecalis using propidium monoazide in combination with real-time PCR
Sinyoung Kim, Seungjong Lee, Euiseong Kim, Deoggyu Seo, Yoonjung Song, Ilyoung Jung
J Korean Acad Conserv Dent 2008;33(6):537-544.   Published online November 30, 2008
DOI: https://doi.org/10.5395/JKACD.2008.33.6.537
AbstractAbstract PDFPubReaderePub

Polymerase chain reaction (PCR) can detect bacteria more rapidly than conventional plate counting. However DNA-based assays cannot distinguish between viable and dead cells due to persistence of DNA after cells have lost their vitality. Recently, propidium monoazide (PMA) treatment has been introduced. The purpose of this study is to evaluate the applicability of the PMA treatment and real-time PCR method for cell counting in comparison with plate counting and to evaluate the antibacterial efficacy of 2% CHX on E. faecalis using PMA treatment in combination with real-time PCR.

Firstly, to elucidate the relationship between the proportion of viable cells and the real-time PCR signals after PMA treatment, mixtures with different ratios of viable and dead cells were used. Secondly, relative difference of viable cells using PMA treatment in combination with real-time PCR was compared with CFU by plate counting. Lastly, antibacterial efficacy of 2% CHX on E. faecalis was measured using PMA treatment in combination with real-time PCR.

The results were as follows :

Ct value increased with decreasing proportion of viable E. faecalis.

There was correlation between viable cells measured by real-time PCR after PMA treatment and CFU by plate counting until Optical density (OD) value remains under 1.0. However, viable cells measured by real-time PCR after PMA treatment have decreased at 1.5 of OD value while CFU kept increasing.

Relative difference of viable E. faecalis decreased more after longer application of 2% CHX.

Citations

Citations to this article as recorded by  
  • Effects of sodium hypochlorite on the potential infectivity of human norovirus GII .4 using propidium monoazide with RT‐qPCR and quality assessments in Manila clams ( Ruditapes phili
    Min Gyu Song, Eun Bi Jeon, Ji Yoon Kim, Shin Young Park
    Journal of Food Processing and Preservation.2022;[Epub]     CrossRef
  • Effect of temperature and medium on the viability ofMycobacterium lepraeduring long term-storage
    Jin-Ho Park, Yun-Ji Kim, Jong-Pill Kim
    Korean Leprosy Bulletin.2019; 52(1): 41.     CrossRef
  • In Vivo Quantitative Evaluation of Live and Dead Bacteria in Root Canal Infection by Using Propidium Monoazide with Real-Time PCR
    Sin-Young Kim, Yooseok Shin, Chan-Young Lee, Il-Young Jung
    Journal of Endodontics.2013; 39(11): 1359.     CrossRef
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Detection of methicillin or vancomycin-resistant Staphylococcus aureus from dental hospital
Jung-Hee Min, Soon-Nang Park, Ho-Keel Hwang, Jung-Beum Min, Hwa-Sook Kim, Joong-Ki Kook
J Korean Acad Conserv Dent 2007;32(2):102-110.   Published online March 31, 2007
DOI: https://doi.org/10.5395/JKACD.2007.32.2.102
AbstractAbstract PDFPubReaderePub

The purpose of this study was to obtain the basic information for the improvement of dental environment by investigating the presence of methicillin- or vancomycin-resistant Staphylococcus aureus (MRSA or VRSA) isolated from dental health care workers (DHCWs) and environment of the Chosun University Dental Hospital (CUDH) and a private dental clinic (control group). Staphylococcus aureus (S. aureus) was isolated from anterior nares of 42 DHCWs and 38 sites, unit chairs, x-ray devices, computers, etc., at 10 departments of the CUDH and 20 DHCWs and 11 sites at the private dental clinic. S. aureus was isolated on mannitol salt agar plate and confirmed by PCR with S. aureus species-specific primer. Antimicrobial susceptibility test of clinical isolates of S. aureus against several antibiotics including methicillin (oxacillin) was performed by investigating minimum inhibitory concentration (MIC) using broth microdilution assay. In addition, PCR was performed to detect the methicillin- or vancomycin-resistant gene. The data showed that one strain of S. aureus was isolated from DHCWs of the CUDH and three strains of S. aureus was isolated from 3 samples of the private dental clinic, respectively. All of the isolates from the CUDH and the private dental clinic had resistance to penicillin G, amoxicillin and vancomycin and susceptibility to oxacillin and ciprofloxacin. The S. aureus strains were already obtained the resistance to penicillin G and amoxicillin. These results suggest that two dental clinics were under relatively safe environment.

Citations

Citations to this article as recorded by  
  • Survey of Staphylococcus epidermidis Contamination on the Hands of Dental Hygienists and Equipment Surface of Dental Clinics
    Seol-Hee Kim
    Journal of Dental Hygiene Science.2017; 17(6): 472.     CrossRef
  • Antimicrobial susceptibility and pathogenic genes ofStaphylococcus aureusisolated from the oral cavity of patients with periodontitis
    Ga-Yeon Kim, Chong Heon Lee
    Journal of Periodontal & Implant Science.2015; 45(6): 223.     CrossRef
  • A Study Regarding Bacterial Contamination of Surfaces in Dental Offices
    Kyoung-Ok Yun, Hye-Young Kim
    Korean Journal of Clinical Laboratory Science.2015; 47(4): 279.     CrossRef
  • A Study on Bacterial Concentrations in Dental Offices
    Kyoung-Ok Yun, Hee-Jin Park, Bu-Soon Son
    Korean Journal of Environmental Health Sciences.2014; 40(6): 469.     CrossRef
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Assessment of sterilization effect and the alteration of surface texture and physical properties of gutta-percha cone after short-term chemical disinfection
Nan-Sim Pang, Il-Young Jung, Yoon-Jung Yu, Kee-Yeon Kum
J Korean Acad Conserv Dent 2006;31(2):133-140.   Published online March 31, 2006
DOI: https://doi.org/10.5395/JKACD.2006.31.2.133
AbstractAbstract PDFPubReaderePub

The purposes of this study were firstly to identify the microbial species on gutta-percha (GP) cones exposed at clinics using polymerase chain reaction, and secondly to evaluate the short-term sterilization effect of three chemical disinfectants. It also evaluated the alteration of surface texture and physical properties of GP cones after 5-min soaking into three chemical disinfectants. 150 GP cones from two endodontic departments were randomly selected for microbial detection using PCR assay with universal primer. After inoculation on the sterilized GP cones with the same microorganism identified by PCR assay, they were soaked in three chemical disinfectants: 5% NaOCl, 2% Chlorhexidine, and ChloraPrep for 1, 5, 10, and 30 minutes. The sterilization effect was evaluated by turbidity and subculture. The change of surface textures using a scanning electron microscope and the tensile strength and elongation rate of the GP cones were measured using an Instron 5500 (Canton). Statistical analysis was performed.

Four bacterial species were detected in 29 GP cones (19.4%), and all the species belonged to the genus Staphylococcus. All chemical disinfectants were effective in sterilization with just 1 minute soaking. On the SEM picture of NaOCl-soaked GP cone, a cluster of cuboidal crystals was seen on the cone surface. The tensile strength of NaOCl-soaked group was significantly higher than the other groups (p < 0.05). Also, all disinfectants significantly increased the elongation rate of GP cones compared to the fresh GP cone (p < 0.05). Present data demonstrate that three chemical disinfectants are useful for rapid sterilization of GP cone just before obturation.

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Distribution of oral pathogens in infections of endodontic origin
Seung-Yoon Kim, Ho-Young Choi, Sang-Hyuk Park, Gi-Woon Choi
J Korean Acad Conserv Dent 2003;28(4):303-313.   Published online July 31, 2003
DOI: https://doi.org/10.5395/JKACD.2003.28.4.303
AbstractAbstract PDFPubReaderePub

It has been documented that periodontopathic bacteria are also implicated in endodontic infections. 16S rDNA gene-directed PCR was to examine the prevalence of periodontopathic bacteria including Actinobacillus actinomycetemcomitans (Aa), Prevotella intermedia (Pi), Prevotella nigrescens (Pn), Porphyromonas gingivalis (Pg), Porphyromonas endodontalis (Pe), and Treponema denticola (Td) in the root canals of 36 endodontically infected teeth having apical lesions with or without clinical symptoms like pain, swelling, and fistula.

In 36 infected root canals, most frequently detected bacterial species was Pg (61.1%), followed by Td (52.8%) and Pe (38.9%).

Of 36 infected root canals, Aa was detected in 6 canals (16.7%) of the teeth, all of which showed clinical symptoms.

Of 36 infected root canals, Pi and Pn were found in 4 (13.9%) and 5 (33.3%), respectively. Notably, prevalence of Pn in the symptomatic teeth was 50.0%.

One of black-pigmented anaerobic bacteria (BPB) including Pi, Pn, Pe, and Pg was detected in all of the teeth that showed pain or especially swelling but not fistula. It was, however, found that prevalence of BPB in the asymptomatic teeth or the teeth with fistula was only 40%.

Pe and Pg were detected in the teeth regardless of the presence or absence of symptoms.

Td was detected in the teeth regardless of the presence or absence of symptoms.

High prevalence of BPB in the symptomatic teeth but low in the asymptomatic teeth suggests that BPB may play an important role in the pathogenesis of periapical lesions.

Citations

Citations to this article as recorded by  
  • Isolation of Propionibacterium acnes among the microbiota of primary endodontic infections with and without intraoral communication
    Sadia Ambreen Niazi, Hana Suleiman Al Kharusi, Shanon Patel, Kenneth Bruce, David Beighton, Federico Foschi, Francesco Mannocci
    Clinical Oral Investigations.2016; 20(8): 2149.     CrossRef
  • Antimicrobial Activity of Isothiocyanates (ITCs) Extracted from Horseradish (Armoracia rusticana) Root against Oral Microorganisms
    HO-WON PARK, KYU-DUCK CHOI, IL-SHIK SHIN
    Biocontrol Science.2013; 18(3): 163.     CrossRef
  • Microbial profile of asymptomatic and symptomatic teeth with primary endodontic infections by pyrosequencing
    Sang-Min Lim, Tae-Kwon Lee, Eun-Jeong Kim, Jun-Hong Park, Yoon Lee, Kwang-Shik Bae, Kee-Yeon Kum
    Journal of Korean Academy of Conservative Dentistry.2011; 36(6): 498.     CrossRef
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Identification of putative pathogens in acute endodontic infections by PCR based on 16S rDNA
Jee-Hoon Kim, So Young Yoo, Sun-A Lim, Joong-Ki Kook, Sang-Soo Lim, Seul-Hee Park, Ho-Keel Hwang
J Korean Acad Conserv Dent 2003;28(2):178-183.   Published online March 31, 2003
DOI: https://doi.org/10.5395/JKACD.2003.28.2.178
AbstractAbstract PDFPubReaderePub

The purpose of this study was to investigate the frequency of 7 putative pathogens in endodontic infections. The specimens were collected from infected pulpal tissue of patients who were referred for root canal treatment to the department of conservative dentistry, Chosun University. Samples were collected aseptically using a barbed broach and a paper point. The cut barbed broaches and paper points were transferred to an eppendorf tube containing 500 ml of 1 X PBS. DNAs were extracted from the samples by direct DNA extraction method using lysis buffer (0.5% EDTA, 1% Triton X-100). Identification of 7 putative pathogens was performed by PCR based on 16S rDNA. The target species were as follows: Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Bacteroides forsythus, Actinobacillus actinomycetemcomitans, and Treponema denticola. Our data revealed that the prevalence of P. endodontalis was found in 88.6% (39/54), P. gingivalis 52.3% (23/44), P. nigrescens 18.2% (8/44), P. intermedia 15.9% (7/44), B. forsythus 18.2% (8/44), A. actinomycetemcomitans 2.3% (1/44), T. denticola 25% (11/44) of the samples. The high prevalence of P. endodontalis and P. gingivalis suggests that they may play an important role in the etiology of endodontic infections.

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