Restorative Dentistry & Endodontics

Seung-Hoon Han

2 Articles

Micro-CT evaluation of internal adaptation in resin fillings with different dentin adhesives: Seung-Hoon Han, Sung-Ho Park; Restor Dent Endod 2014;39(1):24-31. Published online January 20, 2014; DOI: https://doi.org/10.5395/rde.2014.39.1.24

Objectives

The purpose of present study was to evaluate the internal adaptation of composite restorations using different adhesive systems.

Materials and Methods

Typical class I cavities were prepared in 32 human third molars. The teeth were divided into the following four groups: 3-step etch-and-rinse, 2-step etch-and-rinse, 2-step self-etch and 1-step self-etch system were used. After the dentin adhesives were applied, composite resins were filled and light-cured in two layers. Then, silver nitrate solution was infiltrated, and all of the samples were scanned by micro-CT before and after thermo-mechanical load cycling. For each image, the length to which silver nitrate infiltrated, as a percentage of the whole pulpal floor length, was calculated (%SP). To evaluate the internal adaptation using conventional method, the samples were cut into 3 pieces by two sectioning at an interval of 1 mm in the middle of the cavity and they were dyed with Rhodamine-B. The cross sections of the specimens were examined by stereomicroscope. The lengths of the parts where actual leakage was shown were measured and calculated as a percentage of real leakage (%RP). The values for %SP and %RP were compared.

Results

After thermo-mechanical loading, all specimens showed significantly increased %SP compared to before thermo-mechanical loading and 1-step self-etch system had the highest %SP (p < 0.05). There was a tendency for %SP and %RP to show similar microleakage percentage depending on its sectioning.

Conclusions

After thermo-mechanical load cycling, there were differences in internal adaptation among the groups using different adhesive systems.

Citations

Citations to this article as recorded by

Non-Destructive In Vitro Evaluation of an Internal Adaptation of Recent Pulp-Capping Materials in Permanent Teeth Using OCT and Micro-CT
Ahmed Y. Alzahrani, Amani A. Al Tuwirqi, Nada O. Bamashmous, Turki A. Bakhsh, Eman A. El Ashiry
Children.2023; 10(8): 1318. CrossRef
Internal Adaptation of Cusp-weakened Class I Preparations Restored with Bulk-fill, Bi-layered, and Incremental Restorative Techniques: A Micro-CT Analysis
DH Floriani, RN Rached, SA Ignácio, EM Souza
Operative Dentistry.2022; 47(5): 527. CrossRef
An in vitro micro-CT assessment of bioactive restorative materials interfacial adaptation to dentin
Priyanka Angadala, Jyothi Mandava, Ravichandra Ravi, KoteswarRao Hanumanthu, Prasanthi Penmatsa, Hema Pulidindi
Dental Research Journal.2022; 19(1): 56. CrossRef
Tomographic Evaluation of the Internal Adaptation for Recent Calcium Silicate‐Based Pulp Capping Materials in Primary Teeth
A. A. Al Tuwirqi, E. A. El Ashiry, A. Y. Alzahrani, N. Bamashmous, T. A. Bakhsh, Iole Vozza
BioMed Research International.2021;[Epub] CrossRef
Micro-computed tomography in preventive and restorative dental research: A review
Mehrsima Ghavami-Lahiji, Reza Tayefeh Davalloo, Gelareh Tajziehchi, Paria Shams
Imaging Science in Dentistry.2021; 51(4): 341. CrossRef
Validation of a method of quantifying 3D leakage in dental restorations
Fabio A.P. Rizzante, Rana A.F. Sedky, Adilson Y. Furuse, Sorin Teich, Sérgio K. Ishikiriama, Gustavo Mendonça
The Journal of Prosthetic Dentistry.2020; 123(6): 839. CrossRef
Comparison of micro-CT and conventional dye penetration for microleakage assessment after different aging conditions
Rayssa Ferreira Zanatta, Annette Wiegand, Christian Dullin, Alessandra Bühler Borges, Carlos Rocha Gomes Torres, Marta Rizk
International Journal of Adhesion and Adhesives.2019; 89: 161. CrossRef
Comparison of Internal Adaptation of Bulk-fill and Increment-fill Resin Composite Materials
FS Alqudaihi, NB Cook, KE Diefenderfer, MC Bottino, JA Platt
Operative Dentistry.2019; 44(1): E32. CrossRef
Effects of occlusal cavity configuration on 3D shrinkage vectors in a flowable composite
Dalia Kaisarly, Moataz El Gezawi, Guangyun Lai, Jian Jin, Peter Rösch, Karl-Heinz Kunzelmann
Clinical Oral Investigations.2018; 22(5): 2047. CrossRef
Bonding Strategies of Resin Cement to Er, Cr:YSGG Lased Dentin: Micro-CT Evaluation and Microshear Bond Strength Testing
Gökçe Meriç, Simge Taşar, Kaan Orhan
The International Journal of Artificial Organs.2016; 39(2): 72. CrossRef
Calcium hypochlorite as a dentin deproteinization agent: Microleakage, scanning electron microscopy and elemental analysis
Michele Bortoluzzi de Conto Ferreira, Bruno Carlini Júnior, Daniel Galafassi, Delton Luiz Gobbi
Microscopy Research and Technique.2015; 78(8): 676. CrossRef

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THE EFFICACY OF PROGRAMMED CRYO-PRESERVATION UNDER PRESSURE IN RAT PERIODONTAL LIGAMENT CELLS: Young-Eun Lee, Eui-Seong Kim, Jin Kim, Seung-Hoon Han, Seung-Jong Lee; J Korean Acad Conserv Dent 2009;34(4):356-363. Published online January 14, 2009; DOI: https://doi.org/10.5395/JKACD.2009.34.4.356

Abstract PDF PubReader ePub

Abstract

The purpose of this study was to evaluate the viability of periodontal ligament cells in rat teeth using slow cryo-preservation method under pressure by means of MTT assay and WST-1 assay. Eighteen teeth of Sprague-Dawley white female rats of 4 week-old were used for each group.

Both sides of the first and second maxillary molars were extracted as atraumatically as possible under Tiletamine anesthesia. The experimental groups were group 1 (Immediate control), group 2 (Cold preservation at 4°C for 1 week), group 3 (Slow freezing), group 4 (Slow freezing under pressure of 3 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in 37°C water bath, then MTT assay and WST-1 assay were processed. One way ANOVA and Tukey method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization.

In both MTT and WST-1 assay, group 4 showed significantly higher viability of periodontal ligament cells than group 2 and 3 (p < 0.05), but showed lower viability than immediate control group.

By the results of this study, slow cryo-preservation method under pressure suggests the possibility for long term cryo-preservation of the teeth.

Citations

Citations to this article as recorded by

Effects of Slow Programmable Cryopreservation on Preserving Viability of the Cultured Periodontal Ligament Cells from Human Impacted Third Molar
Jin-Woo Kim, Tae-Yi Kim, Ye-mi Kim, Eun-Kyoung Pang, Sun-Jong Kim
Journal of Korean Dental Science.2015; 8(2): 57. CrossRef
The evaluation of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure
Jin-Ho Chung, Jin Kim, Seong-Ho Choi, Eui-Seong Kim, Jiyong Park, Seung-Jong Lee
Journal of Korean Academy of Conservative Dentistry.2010; 35(4): 285. CrossRef
Comparison of viability of oral epithelial cells stored by different freezing methods
Do-Young Baek, Seung-Jong Lee, Han-Sung Jung, EuiSeong Kim
Journal of Korean Academy of Conservative Dentistry.2009; 34(6): 491. CrossRef

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