Restorative Dentistry & Endodontics

Sang-Min Lim

6 Articles

Antifungal effects of synthetic human β-defensin 3-C15 peptide: Sang-Min Lim, Ki-Bum Ahn, Christine Kim, Jong-Won Kum, Hiran Perinpanayagam, Yu Gu, Yeon-Jee Yoo, Seok Woo Chang, Seung Hyun Han, Won-Jun Shon, Woocheol Lee, Seung-Ho Baek, Qiang Zhu, Kee-Yeon Kum; Restor Dent Endod 2016;41(2):91-97. Published online March 17, 2016; DOI: https://doi.org/10.5395/rde.2016.41.2.91

Objectives

The purpose of this ex vivo study was to compare the antifungal activity of a synthetic peptide consisting of 15 amino acids at the C-terminus of human β-defensin 3 (HBD3-C15) with calcium hydroxide (CH) and Nystatin (Nys) against Candida albicans (C. albicans) biofilm.

Materials and Methods

C. albicans were grown on cover glass bottom dishes or human dentin disks for 48 hr, and then treated with HBD3-C15 (0, 12.5, 25, 50, 100, 150, 200, and 300 µg/mL), CH (100 µg/mL), and Nys (20 µg/mL) for 7 days at 37℃. On cover glass, live and dead cells in the biomass were measured by the FilmTracer Biofilm viability assay, and observed by confocal laser scanning microscopy (CLSM). On dentin, normal, diminished and ruptured cells were observed by field-emission scanning electron microscopy (FE-SEM). The results were subjected to a two-tailed t-test, a one way analysis variance and a post hoc test at a significance level of p = 0.05.

Results

C. albicans survival on dentin was inhibited by HBD3-C15 in a dose-dependent manner. There were fewer aggregations of C. albicans in the groups of Nys and HBD3-C15 (≥ 100 µg/mL). CLSM showed C. albicans survival was reduced by HBD3-C15 in a dose dependent manner. Nys and HBD3-C15 (≥ 100 µg/mL) showed significant fungicidal activity compared to CH group (p < 0.05).

Conclusions

Synthetic HBD3-C15 peptide (≥ 100 µg/mL) and Nys exhibited significantly higher antifungal activity than CH against C. albicans by inhibiting cell survival and biofilm.

Citations

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Harnessing antimicrobial peptides in endodontics
Xinzi Kong, Vijetha Vishwanath, Prasanna Neelakantan, Zhou Ye
International Endodontic Journal.2024; 57(7): 815. CrossRef
Human β-defensins and their synthetic analogs: Natural defenders and prospective new drugs of oral health
Mumian Chen, Zihe Hu, Jue Shi, Zhijian Xie
Life Sciences.2024; 346: 122591. CrossRef
Candida albicans Virulence Factors and Pathogenicity for Endodontic Infections
Yeon-Jee Yoo, A Reum Kim, Hiran Perinpanayagam, Seung Hyun Han, Kee-Yeon Kum
Microorganisms.2020; 8(9): 1300. CrossRef
Innate Inspiration: Antifungal Peptides and Other Immunotherapeutics From the Host Immune Response
Derry K. Mercer, Deborah A. O'Neil
Frontiers in Immunology.2020;[Epub] CrossRef
Human salivary proteins and their peptidomimetics: Values of function, early diagnosis, and therapeutic potential in combating dental caries
Kun Wang, Xuedong Zhou, Wei Li, Linglin Zhang
Archives of Oral Biology.2019; 99: 31. CrossRef
Endodontic biofilms: contemporary and future treatment options
Yeon-Jee Yoo, Hiran Perinpanayagam, Soram Oh, A-Reum Kim, Seung-Hyun Han, Kee-Yeon Kum
Restorative Dentistry & Endodontics.2019;[Epub] CrossRef
Bioactive Peptides Against Fungal Biofilms
Karen G. N. Oshiro, Gisele Rodrigues, Bruna Estéfani D. Monges, Marlon Henrique Cardoso, Octávio Luiz Franco
Frontiers in Microbiology.2019;[Epub] CrossRef
Anticandidal Potential of Stem Bark Extract from Schima superba and the Identification of Its Major Anticandidal Compound
Chun Wu, Hong-Tan Wu, Qing Wang, Guey-Horng Wang, Xue Yi, Yu-Pei Chen, Guang-Xiong Zhou
Molecules.2019; 24(8): 1587. CrossRef
Synthetic Human β Defensin-3-C15 Peptide in Endodontics: Potential Therapeutic Agent in Streptococcus gordonii Lipoprotein-Stimulated Human Dental Pulp-Derived Cells
Yeon-Jee Yoo, Hiran Perinpanayagam, Jue-Yeon Lee, Soram Oh, Yu Gu, A-Reum Kim, Seok-Woo Chang, Seung-Ho Baek, Kee-Yeon Kum
International Journal of Molecular Sciences.2019; 21(1): 71. CrossRef
Candida Infections and Therapeutic Strategies: Mechanisms of Action for Traditional and Alternative Agents
Giselle C. de Oliveira Santos, Cleydlenne C. Vasconcelos, Alberto J. O. Lopes, Maria do S. de Sousa Cartágenes, Allan K. D. B. Filho, Flávia R. F. do Nascimento, Ricardo M. Ramos, Emygdia R. R. B. Pires, Marcelo S. de Andrade, Flaviane M. G. Rocha, Cristi
Frontiers in Microbiology.2018;[Epub] CrossRef
Perspectives for clinical use of engineered human host defense antimicrobial peptides
María Eugenia Pachón-Ibáñez, Younes Smani, Jerónimo Pachón, Javier Sánchez-Céspedes
FEMS Microbiology Reviews.2017; 41(3): 323. CrossRef
The synthetic human beta-defensin-3 C15 peptide exhibits antimicrobial activity against Streptococcus mutans, both alone and in combination with dental disinfectants
Ki Bum Ahn, A. Reum Kim, Kee-Yeon Kum, Cheol-Heui Yun, Seung Hyun Han
Journal of Microbiology.2017; 55(10): 830. CrossRef
Antibiofilm peptides against oral biofilms
Zhejun Wang, Ya Shen, Markus Haapasalo
Journal of Oral Microbiology.2017; 9(1): 1327308. CrossRef
Humanβ-Defensin 3 Reduces TNF-α-Induced Inflammation and Monocyte Adhesion in Human Umbilical Vein Endothelial Cells
Tianying Bian, Houxuan Li, Qian Zhou, Can Ni, Yangheng Zhang, Fuhua Yan
Mediators of Inflammation.2017; 2017: 1. CrossRef
Antifungal Effects of Synthetic Human Beta-defensin-3-C15 Peptide on Candida albicans –infected Root Dentin
Yeon-Jee Yoo, Ikyung Kwon, So-Ram Oh, Hiran Perinpanayagam, Sang-Min Lim, Ki-Bum Ahn, Yoon Lee, Seung-Hyun Han, Seok-Woo Chang, Seung-Ho Baek, Qiang Zhu, Kee-Yeon Kum
Journal of Endodontics.2017; 43(11): 1857. CrossRef
A 15-amino acid C-terminal peptide of beta-defensin-3 inhibits bone resorption by inhibiting the osteoclast differentiation and disrupting podosome belt formation
Ok-Jin Park, Jiseon Kim, Ki Bum Ahn, Jue Yeon Lee, Yoon-Jeong Park, Kee-Yeon Kum, Cheol-Heui Yun, Seung Hyun Han
Journal of Molecular Medicine.2017; 95(12): 1315. CrossRef

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Effect of acidic solutions on the microhardness of dentin and set OrthoMTA and their cytotoxicity on murine macrophage: Soram Oh, Hiran Perinpanayagam, Yoon Lee, Jae-Won Kum, Yeon-Jee Yoo, Sang-Min Lim, Seok Woo Chang, Won-Jun Shon, Woocheol Lee, Seung-Ho Baek, Kee-Yeon Kum; Restor Dent Endod 2016;41(1):12-21. Published online December 1, 2015; DOI: https://doi.org/10.5395/rde.2016.41.1.12

Abstract PDF PubReader ePub

Objectives

To evaluate the effects of three acids on the microhardness of set mineral trioxide aggregate (MTA) and root dentin, and cytotoxicity on murine macrophage.

Materials and Methods

OrthoMTA (BioMTA) was mixed and packed into the human root dentin blocks of 1.5 mm diameter and 5 mm height. Four groups, each of ten roots, were exposed to 10% citric acid (CA), 5% glycolic acid (GA), 17% ethylenediaminetetraacetic acid (EDTA), and saline for five minutes after setting of the OrthoMTA. Vickers surface microhardness of set MTA and dentin was measured before and after exposure to solutions, and compared between groups using one-way ANOVA with Tukey test. The microhardness value of each group was analyzed using student t test. Acid-treated OrthoMTA and dentin was examined by scanning electron microscope (SEM). Cell viability of tested solutions was assessed using WST-8 assay and murine macrophage.

Results

Three test solutions reduced microhardness of dentin. 17% EDTA demonstrated severe dentinal erosion, significantly reduced the dentinal microhardness compared to 10% CA (p = 0.034) or 5% GA (p = 0.006). 10% CA or 5% GA significantly reduced the surface microhardness of set MTA compared to 17% EDTA and saline (p < 0.001). Acid-treated OrthoMTA demonstrated microporous structure with destruction of globular crystal. EDTA exhibited significantly more cellular toxicity than the other acidic solutions at diluted concentrations (0.2, 0.5, 1.0%).

Conclusions

Tested acidic solutions reduced microhardness of root dentin. Five minute's application of 10% CA and 5% GA significantly reduced the microhardness of set OrthoMTA with lower cellular cytotoxicity compared to 17% EDTA.

Citations

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Impact of calcium hydroxide and 2-hydroxyisocaproic acid on the microhardness of root dentine: an in vitro study
Nandini T. Niranjan, Protim Ghosh Dastidar, Raghavendra Penukonda, Galvin Sim Siang Lin, Roopa Babannavar, Arun Jaysheel, Harshada Pattar
Odontology.2024; 112(3): 711. CrossRef
Evaluation of the Effect of Chitosan-Based Irrigation Solutions on the Bond Strength of Mineral Trioxide Aggregate to Bulk-Fill Composite
Arzu Şahin Mantı, Bağdagül Helvacıoğlu Kıvanç
Journal of Functional Biomaterials.2024; 15(12): 370. CrossRef
Effect of Various Acid Solutions as an Aid in Removing the OrthoMTA-Based Root Canal Filling
Naveen Chhabra, Abhishek Parolia
Materials.2023; 16(13): 4535. CrossRef
Effect of Glycolic Acid, Maleic Acid, and EDTA in the Removal of Smear Layer from Root Canal Dentin
Tarini Mullick, Nidambur Vasudev Ballal
Pesquisa Brasileira em Odontopediatria e Clínica Integrada.2023;[Epub] CrossRef
A comparative evaluation of the effect of various chelating agents on the microhardness of root canal dentin: An in vitro study
Mineet Kaul, Zinnie Nanda, Kranthikumar Reddy, Rahul Deore, Divya Mandlecha, Esha Jaiswal
Endodontology.2023; 35(3): 234. CrossRef
Calcium hydroxide and niobium pentoxide treatment effects before MTA placement
Kolli Sankeerthana, Kittappa Karthikeyan, Sekar Mahalaxmi
Australian Endodontic Journal.2023; 49(1): 48. CrossRef
Calcium silicate and calcium aluminate cements for dentistry reviewed
Carolyn Primus, James L. Gutmann, Franklin R. Tay, Anna B. Fuks
Journal of the American Ceramic Society.2022; 105(3): 1841. CrossRef
Influence of Acidic Environmental Conditions on Push-Out Bonding Strength of Four Calcium Silicate-Based Materials to Root Dentin
Beliz Özel, Raif Erişen, Boonlert Kukiattrakoon
International Journal of Dentistry.2022; 2022: 1. CrossRef
The effects of sodium hypochlorite and ethylenediaminetetraacetic acid on the microhardness of Mineral Trioxide Aggregate and TotalFill Bioceramic Putty
Jacklyn H.R. Chu, Kalie Y. Chia, Alexander L. Qui, Alex Moule, William N. Ha
Australian Endodontic Journal.2020; 46(1): 33. CrossRef
Pre-application of dentin bonding agent prevents discoloration caused by mineral trioxide aggregate
Yoo-Lim Choi, Young-Eun Jang, Bom Sahn Kim, Jin-Woo Kim, Yemi Kim
BMC Oral Health.2020;[Epub] CrossRef
Glycolic acid as the final irrigant in endodontics: Mechanical and cytotoxic effects
Yuri Dal Bello, Hisadora Fracaro Porsch, Ana Paula Farina, Matheus Albino Souza, Emmanuel João Nogueira Leal Silva, Ana Karina Bedran-Russo, Doglas Cecchin
Materials Science and Engineering: C.2019; 100: 323. CrossRef
Carbohydrate-electrolyte drinks exhibit risks for human enamel surface loss
Mary Anne Sampaio de Melo, Vanara Florêncio Passos, Juliana Paiva Marques Lima, Sérgio Lima Santiago, Lidiany Karla Azevedo Rodrigues
Restorative Dentistry & Endodontics.2016; 41(4): 246. CrossRef

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Microbial profile of asymptomatic and symptomatic teeth with primary endodontic infections by pyrosequencing: Sang-Min Lim, Tae-Kwon Lee, Eun-Jeong Kim, Jun-Hong Park, Yoon Lee, Kwang-Shik Bae, Kee-Yeon Kum; J Korean Acad Conserv Dent 2011;36(6):498-505. Published online November 30, 2011; DOI: https://doi.org/10.5395/JKACD.2011.36.6.498

Abstract PDF PubReader ePub

Objectives

The purpose of this in vivo study was to investigate the microbial diversity in symptomatic and asymptomatic canals with primary endodontic infections by using GS FLX Titanium pyrosequencing.

Materials and Methods

Sequencing was performed on 6 teeth (symptomatic, n = 3; asymptomatic, n = 3) with primary endodontic infections. Amplicons from hypervariable region of the small-subunit ribosomal RNA gene were generated by polymerized chain reaction (PCR), and sequenced by means of the GS FLX Titanium pyrosequencing.

Results

On average, 10,639 and 45,455 16S rRNA sequences for asymptomatic and symptomatic teeth were obtained, respectively. Based on Ribosomal Database Project Classifier analysis, pyrosequencing identified the 141 bacterial genera in 13 phyla. The vast majority of sequences belonged to one of the seven phyla: Actinobacteria, Bacteroidetes, Firmicutes, Fusobacteria, Proteobacteria, Spirochetes, and Synergistetes. In genus level, Pyramidobacter, Streptococcus, and Leptotrichia constituted about 50% of microbial profile in asymptomatic teeth, whereas Neisseria, Propionibacterium, and Tessaracoccus were frequently found in symptomatic teeth (69%). Grouping the sequences in operational taxonomic units (3%) yielded 450 and 1,997 species level phylotypes in asymptomatic and symptomatic teeth, respectively. The total bacteria counts were significantly higher in symptomatic teeth than that of asymptomatic teeth (p < 0.05).

Conclusions

GS FLX Titanium pyrosequencing could reveal a previously unidentified high bacterial diversity in primary endodontic infections.

Citations

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Root Canal Microbiome Associated With Asymptomatic Apical Periodontitis as Determined by High-Throughput Sequencing
Rodrigo Rodrigues Amaral, Tiago Braga, José F. Siqueira, Isabela N. Rôças, Caio Tavora Coelho da Costa Rachid, Anna Gabriella Guimarães Oliveira, Maria Ilma de Souza Côrtes, Robert Mattew Love
Journal of Endodontics.2022; 48(4): 487. CrossRef
A critical analysis of research methods and experimental models to study the root canal microbiome
José F. Siqueira, Isabela N. Rôças
International Endodontic Journal.2022; 55(S1): 46. CrossRef
Oral microbiomes in children with asthma and dental caries
Sergey V. Cherkasov, Larisa Yu. Popova, Tatyana V. Vivtanenko, Rimma R. Demina, Yuri A. Khlopko, Alexander S. Balkin, Andrey O. Plotnikov
Oral Diseases.2019; 25(3): 898. CrossRef
Insights into the human oral microbiome
Digvijay Verma, Pankaj Kumar Garg, Ashok Kumar Dubey
Archives of Microbiology.2018; 200(4): 525. CrossRef
Solving the etiology of dental caries
Aurea Simón-Soro, Alex Mira
Trends in Microbiology.2015; 23(2): 76. CrossRef
Present status and future directions in endodontic microbiology
José F. Siqueira, Isabela N. Rôças
Endodontic Topics.2014; 30(1): 3. CrossRef
Application of high-throughput sequencing in understanding human oral microbiome related with health and disease
Hui Chen, Wen Jiang
Frontiers in Microbiology.2014;[Epub] CrossRef

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Clinical assessment of whitening efficacy and safety of in-office tooth whitening system containing 15% hydrogen peroxide with or without light activation: Young-Suk Noh, Young-Jee Rho, Yeon-Jee Yoo, Hyang-Ok Lee, Sang-Min Lim, Hyun-Jeong Kweon, Yeun Kim, Seong-Yeon Park, Hee-Young Yoon, Jung-Hyun Lee, Chan-Hee Lee, So-Ram Oh, Kee-Yeon Kum; J Korean Acad Conserv Dent 2011;36(4):306-312. Published online July 31, 2011; DOI: https://doi.org/10.5395/JKACD.2011.36.4.306

Abstract PDF PubReader ePub

Objectives

This clinical study evaluated the effect of light activation on the whitening efficacy and safety of in-office bleaching system containing 15% hydrogen peroxide gel.

Materials and Methods

Thirty-three volunteers were randomly treated with (n = 17, experimental group) or without light activation (n = 16, control group), using Zoom2 white gel (15% H₂O₂, Discus Dental) for a total treatment time of 45 min. Visual and instrumental color measurements were obtained using Vitapan Classical shade guide and Shadepilot (DeguDent) at screening test, after bleaching, and 1 month and 3 month after bleaching. Data were analyzed using t-test, repeated measure ANOVA, and chi-squared test.

Results

Zoom2 white gel produced significant shade changes in both experimental and control group when pre-treatment shade was compared with that after bleaching. However, shade difference between two groups was not statistically significant (p > 0.05). Tooth shade relapse was not detected at 3 months after bleaching. The incidence of transient tooth sensitivity was 39.4%, with being no differences between two groups.

Conclusions

The application of light activation with Zoom2 white gel system neither achieved additional whitening effects nor showed more detrimental influences.

Citations

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Effect of preference drinks on tooth color reduction after tooth bleaching: A 12-momth follow-up study
Hyo Jin Goo, Min Jeong Cho, Yun Sook Jung, Ji Hye Kim, Fan Dong, Keun Bae Song
Journal of Korean Academy of Oral Health.2016; 40(1): 55. CrossRef
Development of a Piezoelectric Ultrasonic Tooth-whitening Apparatus
Young-Jin Lee, Jong-Hoo Paik, Jeong-Bae Lee, Seung-Jae Choi
Transactions on Electrical and Electronic Materials.2013; 14(5): 268. CrossRef

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Analysis of para-chloroaniline after chemical interaction between alexidine and sodium hypochlorite using mass spectrometry: A preliminary study: Hyeon-Sik Kim, Seung-Hyun Han, Soram Oh, Sang-Min Lim, Yu Gu, Kee-Yeon Kum; J Korean Acad Conserv Dent 2010;35(4):295-301. Published online July 31, 2010; DOI: https://doi.org/10.5395/JKACD.2010.35.4.295

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The purposes of this study were firstly to investigate the any formation of precipitate after interaction between ALX and NaOCL and secondarily to analyze the PCA formation by using time of flight secondary ion mass (TOF-SIM) spectrometry. Mass spectrometry analysis was performed for the mixture of 0.5% ALX and 5.25% NaOCl. As controls, 2.5% CHX with 5.25% NaOCl and 1% PCA solutions were used. Any formation of precipitates in 10 tested solutions was evaluated by naked eye. Results of mass spectrum showed that the typical peak of PCA was not detected in mixed solution of ALX and NaOCl, whereas CHX/NaOCl mixture showed the same peak that found in the PCA spectrum. Precipitate formation was only observed in CHX/NaOCL mixture. The present TOF-SIM spectrometry results indicated that ALX can be a useful root canal irrigant combined with NaOCl during canal instrumentation. Further study is necessary to confirm the antimicrobial effect of ALX against endodontic pathogen before its clinical application as an endodontic irrigant.

Citations

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Chemical Interaction of Alexidine and Sodium Hypochlorite
Hyeon-Sik Kim, Qiang Zhu, Seung-Ho Baek, Il-Young Jung, Won-Jun Son, Seok-Woo Chang, Woocheol Lee, Yu Gu, Yoon Lee, Sung-Tae Hong, Kwang-Shik Bae, Ji-Woong Kim, Kun Cho, Kee-Yeon Kum
Journal of Endodontics.2012; 38(1): 112. CrossRef

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Effect of cross-sectional area of 6 nickel-titanium rotary instruments on the fatigue fracture under cyclic flexural stress: A fractographic analysis: Soo-Youn Hwang, So-Ram Oh, Yoon Lee, Sang-Min Lim, Kee-Yeon Kum; J Korean Acad Conserv Dent 2009;34(5):424-429. Published online September 30, 2009; DOI: https://doi.org/10.5395/JKACD.2009.34.5.424

Abstract PDF PubReader ePub: This study aimed to assess the influence of different cross-sectional area on the cyclic fatigue fracture of Ni-Ti rotary files using a fatigue tester incorporating cyclical axial movement. Six brands of Ni-Ti rotary files (ISO 30 size with .04 taper) of 10 each were tested: Alpha system (KOMET), HeroShaper (MicroMega), K3 (SybronEndo), Mtwo (VDW), NRT (Mani), and ProFile (Dentsply). A fatigue-tester (Denbotix) was designed to allow cyclic tension and compressive stress on the tip of the instrument. Each file was mounted on a torque controlled motor (Aseptico) using a 1:20 reduction contra-angle and was rotated at 300 rpm with a continuous, 6 mm axial oscillating motion inside an artificial steel canal. The canal had a 60° angle and a 5 mm radius of curvature. Instrument fracture was visually detected and the time until fracture was recorded by a digital stop watch. The data were analyzed statistically. Fractographic analysis of all fractured surfaces was performed to determine the fracture modes using a scanning electron microscope. Cross-sectional area at 3 mm from the tip of 3 unused Ni-Ti instruments for each group was calculated using Image-Pro Plus (Imagej 1.34n, NIH). Results showed that NRT and ProFile had significantly longer time to fracture compared to the other groups (p < .05). The cross-sectional area was not significantly associated with fatigue resistance. Fractographycally, all fractured surfaces demonstrated a combination of ductile and brittle fracture. In conclusion, there was no significant relationship between fatigue resistance and the cross-sectional area of Ni-Ti instruments under experimental conditions.

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