Restorative Dentistry & Endodontics

Gautam Pyarelal Badole

2 Articles

Evaluation of softening ability of Xylene & Endosolv-R on three different epoxy resin based sealers within 1 to 2 minutes - an in vitro study: Pratima Ramakrishna Shenoi, Gautam Pyarelal Badole, Rajiv Tarachand Khode; Restor Dent Endod 2014;39(1):17-23. Published online January 20, 2014; DOI: https://doi.org/10.5395/rde.2014.39.1.17

Objectives

This study evaluated the efficacy of Endosolv-R and Xylene in softening epoxy resin based sealer after 1 to 2 min exposure.

Materials and Methods

Sixty Teflon molds (6 mm × 1.5 mm in inner diameter and depth) were equally divided into 3 groups of 20 each. AH 26 (Dentsply/De Trey), AH Plus (Dentsply/De Trey), Adseal (Meta-Biomed) were manipulated and placed in the molds allotted to each group and allowed to set at 37℃ in 100% humidity for 2 wk. Each group was further divided into 2 subgroups according to the solvents used, i.e. Xylene (Lobachemie) and Endosolv-R (Septodont). Specimens in each subgroup were exposed to respective solvents for 1 and 2 min and the corresponding Vicker's microhardness (HV) was assessed. Data was analysed by Mauchly's test and two-way analysis of variance (ANOVA) with repeated measures, and one-way ANOVA.

Results

Initial hardness was significantly different among the three sealers with AH Plus having the greatest and Adseal having the least. After 2 min, Xylene softened AH Plus and Adseal sealer to 11% and 25% of their initial microhardness, respectively (p < 0.001), whereas AH 26 was least affected, maintaining 89.4% of its initial microhardness. After 2 min, Endosolv-R softened AH 26, AH Plus and Adseal to 12.7, 5.6 and 8.1% of their initial microhardness, respectively (p < 0.001).

Conclusions

Endosolv-R was a significantly more effective short term softener for all the tested sealers after 2 min whereas Xylene was an effective short term softener against AH plus and Adseal but less effective against AH 26.

Citations

Citations to this article as recorded by

Influência do protocolo de remoção de resíduos de cimentos à base de resina epóxi sobre a interface de adesão com o adesivo universal, utilizado na estratégia condiciona-e-lava
Paulo Firmino Da Costa Neto, Mariana Bena Gelio, Elisângela Maria Pereira De Souza, Jardel Camilo do Carmo Monteiro, Adirson Jorge Júnior, Thais Piragine Leandrin, José Roberto Cury Saad, Milton Carlos Kuga
Cuadernos de Educación y Desarrollo.2023; 15(5): 4802. CrossRef
Comparative evaluation of the effectiveness of various solvents on dissolving efficacy of gutta percha- An in vitro- study
Syed Manzoor Ul Haq Bukhari, Sheeeban Rashid, Rahil Bhat
International Dental Journal of Student's Research.2023; 11(3): 121. CrossRef
Bonding effects of cleaning protocols and time-point of acid etching on dentin impregnated with endodontic sealer
Tatiane Miranda Manzoli, Joissi Ferrari Zaniboni, João Felipe Besegato, Flávia Angélica Guiotti, Andréa Abi Rached Dantas, Milton Carlos Kuga
Restorative Dentistry & Endodontics.2022;[Epub] CrossRef
Dissolving efficacy of xylene on epoxy resin-based and bioceramic-based root canal sealers
Cindy Willie
Scientific Dental Journal.2022; 6(1): 32. CrossRef
A comparative evaluation of removal of gutta percha using two retreatment file system: An in vitro study
Shruthi Mary Sunil, Balakrishnan Rajkumar, Vishesh Gupta, Akanksha Bhatt, Pragyan Paliwal
IP Indian Journal of Conservative and Endodontics.2020; 5(2): 53. CrossRef
Use of Resin Solvent as a Facilitator for Removal of Resin Composite Restoratives by Influencing their Mechanical Properties: Is this Possible?
Inas A Elghandour
Journal of Operative Dentistry & Endodontics.2019; 4(1): 1. CrossRef
Effect of the calcium silicate‐based sealer removal protocols and time‐point of acid etching on the dentin adhesive interface
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Persistence of Residues after Endodontic Retreatment related to the Obturation Technique and to the Solvent
Matheus Coelho Bandéca, Milton C Kuga, Keren CF Jordão-Basso, Mateus R Tonetto, Arturo J Aranda-Garcia, Fernando A Vázquez-Garcia, Suellen NL Lima, Jardel C do Carmo Monteiro
World Journal of Dentistry.2017; 8(1): 41. CrossRef
Retreatability of different endodontic sealers using chemical solvents
M.F. Obeid, M.M. Nagy
Tanta Dental Journal.2015; 12(4): 286. CrossRef
Do the Sealer Solvents Used Affect Apically Extruded Debris in Retreatment?
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A comparative evaluation of cytotoxicity of root canal sealers: an in vitro study: Gautam Pyarelal Badole, Manjusha Madhukar Warhadpande, Ganesh Kothiramji Meshram, Rakesh Namdeoraoji Bahadure, Shubha Gopal Tawani, Gopal Tawani, Shital Gautam Badole; Restor Dent Endod 2013;38(4):204-209. Published online November 12, 2013; DOI: https://doi.org/10.5395/rde.2013.38.4.204

Abstract PDF PubReader ePub

Objectives

The objective of this in vitro study was to evaluate and compare the cytotoxicity of four different root canal sealers i.e. Apexit Plus (Ivoclar Vivadent), Endomethasone N (Septodont), AH-26 (Dentsply) and Pulpdent Root Canal Sealer (Pulpdent), on a mouse fibroblast cell line (L929).

Materials and Methods

Thirty two discs for each sealer (5 mm in diameter and 2 mm in height) were fabricated in Teflon mould. The sealer extraction was made in cell culture medium (Dulbecco's Modified Eagle's Medium, DMEM) using the ratio 1.25 cm²/mL between the surface of the sealer samples and the volume of medium in a shaker incubator. Extraction of each sealer was obtained at 24 hr, 7th day, 14th day, and one month of interval. These extracts were incubated with L929 cell line and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was done. Two-way ANOVA for interaction effects between sealer and time and Post-hoc multiple comparison using Tukey's test across all the 16 different groups were used for statistical analysis.

Results

Apexit Plus root canal sealer was significantly less toxic than other sealers (p < 0.05) and showed higher cellular growth than control. Endomethasone N showed mild cytotoxicity. AH-26 showed severe toxicity which became mild after one month while Pulpdent Root Canal Sealer showed severe to moderate toxicity.

Conclusions

Apexit Plus was relatively biocompatible sealer as compared to other three sealers which were cytotoxic at their initial stages, however, they became biocompatible with time.

Citations

Citations to this article as recorded by

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Comparative analysis of the inflammatory response of human gingival fibroblasts to NeoSEALER Flo and CeraSeal bioceramic sealers: an in vitro study
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Emmanuel Samson, Lata B Gangurde, Jaiprakash R Rathod, Pradnya S Jadhav, Sangeeta Ambhore, Pranav S Jadhav
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Human Gingival Fibroblasts Response to Different Endodontic Sealers: An In Vitro Study
Rita Noites, Inês Tavares, Miguel Cardoso, Isabel M. Carreira, Maria Bartolomeu, Ana S. Duarte, Ilda P. Ribeiro
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Antibacterial and Cytotoxicity of Root Canal Sealer with the Addition of Chitosan Nanoparticle at Various Concentrations
Diatri Nari Ratih, Ema Mulyawati, Rika Kurnia Santi, Yulita Kristanti
European Journal of Dentistry.2023; 17(02): 398. CrossRef
Transforaminal and systemic diffusion of an active agent from a zinc oxide eugenol-based endodontic sealer containing hydrocortisone—in an in vivo model
Davy Aubeux, Anne Valot-Salengro, Gaelle Gautier, Arnaud Malet, Fabienne Pérez
Clinical Oral Investigations.2020; 24(12): 4395. CrossRef
PEGylated curcumin-loaded nanofibrous mats with controlled burst release through bead knot-on-spring design
Mahdi Saeed, Hamid Mirzadeh, Mojgan Zandi, Jalal Barzin
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A New Calcium Silicate-Based Root Canal Dressing: Physical and Chemical Properties, Cytotoxicity and Dentinal Tubule Penetration
Natália Villa, Vanessa Valgas Dos Santos, Ubirajara Maciel da Costa, Aline Teixeira Mendes, Pedro Henrique Marks Duarte, Ricardo Abreu da Rosa, Jefferson Ricardo Pereira, Marcus Vinícius Reis Só
Brazilian Dental Journal.2020; 31(6): 598. CrossRef
Evaluation of the Antimicrobial Efficacy of Herbal Extracts Added to Root Canal Sealers of Different Bases: An In Vitro Study
Abhay M Tripathi, Minarani T Devi, Sonali K Kalra, Ujjala Ghoshal
International Journal of Clinical Pediatric Dentistry.2019; 12(5): 398. CrossRef
Endodontic-related inferior alveolar nerve injuries: A review and a therapeutic flow chart
R. Castro, M. Guivarc'h, J.M. Foletti, J.H. Catherine, C. Chossegros, L. Guyot
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Comparison between direct contact and extract exposure methods for PFO cytotoxicity evaluation
Girish K. Srivastava, Maria L. Alonso-Alonso, Ivan Fernandez-Bueno, Maria T. Garcia-Gutierrez, Fernando Rull, Jesús Medina, Rosa M. Coco, J. Carlos Pastor
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Novel endodontic sealers induce cell cytotoxicity and apoptosis in a dose-dependent behavior and favorable response in mice subcutaneous tissue
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