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How do imaging protocols affect the assessment of root-end fillings?
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Fernanda Ferrari Esteves Torres, Reinhilde Jacobs, Mostafa EzEldeen, Karla de Faria-Vasconcelos, Juliane Maria Guerreiro-Tanomaru, Bernardo Camargo dos Santos, Mário Tanomaru-Filho
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Restor Dent Endod 2022;47(1):e2. Published online December 15, 2021
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DOI: https://doi.org/10.5395/rde.2022.47.e2
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Abstract
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This study investigated the impact of micro-computed tomography (micro-CT)-based voxel size on the analysis of material/dentin interface voids and thickness of different endodontic cements. Materials and MethodsFollowing root-end resection and apical preparation, maxillary premolars were filled with mineral trioxide aggregate (MTA), Biodentine, and intermediate restorative material (IRM) (n = 24). The samples were scanned using micro-CT (SkyScan 1272; Bruker) and the cement/dentin interface and thickness of materials were evaluated at voxel sizes of 5, 10, and 20 µm. Analysis of variance and the Tukey test were conducted, and the degree of agreement between different voxel sizes was evaluated using the Bland and Altman method (p < 0.05). ResultsAll materials showed an increase in thickness from 5 to 10 and 20 µm (p < 0.05). When evaluating the interface voids, materials were similar at 5 µm (p > 0.05), while at 10 and 20 µm Biodentine showed the lowest percentage of voids (p < 0.05). A decrease in the interface voids was observed for MTA and IRM at 20 µm, while Biodentine showed differences among all voxel sizes (p < 0.05). The Bland-Altman plots for comparisons among voxel sizes showed the largest deviations when comparing images between 5 and 20 µm. ConclusionsVoxel size had an impact on the micro-CT evaluation of thickness and interface voids of endodontic materials. All cements exhibited an increase in thickness and a decrease in the void percentage as the voxel size increased, especially when evaluating images at 20 µm.
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Citations
Citations to this article as recorded by 
- Marginal Adaptation and Porosity of a Novel MTA Brand Applied as Root-End Filling Material: A Micro-CT Study
Yaneta Kouzmanova, Ivanka Dimitrova Applied Sciences.2024; 14(7): 2758. CrossRef - Supplementary methods for filling material removal: A systematic review and meta-analysis of micro-CT imaging studies
Bruna Venzke Fischer, Taynara Santos Goulart, Filipe Colombo Vitali, Diego Leonardo de Souza, Cleonice da Silveira Teixeira, Lucas da Fonseca Roberti Garcia Journal of Dentistry.2024; 151: 105445. CrossRef
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A micro-computed tomographic study using a novel test model to assess the filling ability and volumetric changes of bioceramic root repair materials
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Fernanda Ferrari Esteves Torres, Jader Camilo Pinto, Gabriella Oliveira Figueira, Juliane Maria Guerreiro-Tanomaru, Mario Tanomaru-Filho
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Restor Dent Endod 2021;46(1):e2. Published online December 8, 2020
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DOI: https://doi.org/10.5395/rde.2021.46.e2
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Abstract
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- Objectives
New premixed bioceramic root repair materials require moisture for setting. Using micro-computed tomography (micro-CT), this study evaluated the filling ability and volumetric changes of calcium silicate-based repair materials (mineral trioxide aggregate repair high-plasticity [MTA HP] and Bio-C Repair, Angelus), in comparison with a zinc oxide and eugenol-based material (intermediate restorative material [IRM]; Dentsply DeTrey). Materials and MethodsGypsum models with cavities 3 mm deep and 1 mm in diameter were manufactured and scanned using micro-CT (SkyScan 1272. Bruker). The cavities were filled with the cements and scanned again to evaluate their filling capacity. Another scan was performed after immersing the samples in distilled water for 7 days to assess the volumetric changes of the cements. The statistical significance of differences in the data was evaluated using analysis of variance and the Tukey test with a 5% significance level. ResultsBio-C Repair had a greater filling ability than MTA HP (p < 0.05). IRM was similar to Bio-C and MTA HP (p > 0.05). MTA HP presented the largest volumetric change (p < 0.05), showing more volume loss than Bio-C and IRM, which were similar (p > 0.05). ConclusionsBio-C Repair is a new endodontic material with excellent filling capacity and low volumetric change. The gypsum model proposed for evaluating filling ability and volumetric changes by micro-CT had appropriate and reproducible results. This model may enhance the physicochemical evaluation of premixed bioceramic materials, which need moisture for setting.
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Citations
Citations to this article as recorded by 
- Comparative evaluation of sealing potential of mineral trioxide aggregate, biodentine, and bio-C repair in furcation perforations: A glucose penetration study
Ashwija Shetty, Hajira Anjum Sultana, A. Srirekha, C. Champa, Suditi Pal, V. Sahithi Journal of Conservative Dentistry and Endodontics.2025; 28(2): 144. CrossRef - Evaluation of volumetric and surface stability of calcium silicate-based repair cements at different pHs
Ana Cristina Padilha Janini, Débora Leticia Bittencourt Leite Alves, Victor Augusto Benedicto dos Santos, Brenda Fornazaro Moraes, Nilvan Alves da Silva, Matheus Barros-Costa, Luciano Augusto Cano Martins, Francisco Haiter Neto, Marina Angélica Marciano Clinical Oral Investigations.2025;[Epub] CrossRef - Effect of pH on the solubility and volumetric change of ready-to-use Bio-C Repair bioceramic material
Luana Raphael da SILVA, Jader Camilo PINTO, Juliane Maria GUERREIRO-TANOMARU, Mário TANOMARU-FILHO Brazilian Oral Research.2024;[Epub] CrossRef - Effect of blood and artificial saliva contamination on marginal adaptation and sealing ability of different retrograde filling materials: A comparative analysis
Yantrapragada Lakshmi Sunanda, Krishna Prasad Parvathaneni, T. B. V. G. Raju, Abitha Seshadri, Gowtam Dev Dondapati Journal of Conservative Dentistry and Endodontics.2024; 27(7): 743. CrossRef - Marginal Adaptation and Porosity of a Novel MTA Brand Applied as Root-End Filling Material: A Micro-CT Study
Yaneta Kouzmanova, Ivanka Dimitrova Applied Sciences.2024; 14(7): 2758. CrossRef - Volumetric change of calcium silicate-based repair materials in a simulated inflammatory environment: A micro-computed tomography study
Giovanna da Cunha Mendonça, Karina Ines Medina Carita Tavares, Airton Oliveira Santos-Junior, Fernanda Ferrari Esteves Torres, Jáder Camilo Pinto, Juliane Maria Guerreiro-Tanomaru, Mário Tanomaru-Filho Journal of Conservative Dentistry and Endodontics.2024; 27(8): 817. CrossRef - Biocompatibility, bioactivity, porosity, and sealer/dentin interface of bioceramic ready-to-use sealers using a dentin-tube model
Rafaela Nanami Handa Inada, Evelin Carine Alves Silva, Camila Soares Lopes, Marcela Borsatto Queiroz, Fernanda Ferrari Esteves Torres, Guilherme Ferreira da Silva, Paulo Sérgio Cerri, Juliane Maria Guerreiro–Tanomaru, Mário Tanomaru-Filho Scientific Reports.2024;[Epub] CrossRef - Healing the Open Apex: A Case Report on Innovative Apexogenesis of a Maxillary Molar Using Bio-C Repair
Ashwija Shetty, Hajira A Sultana, Keerthan B V, Nithin S Reddy Cureus.2024;[Epub] CrossRef - Evaluation the Marginal Adaptation for the Bio C Repair and Other Root end Filling Material by Using Scanning Electron Microscope (A Comparative In Vitro Study)
Fatimah HAMADHİ, Zainab M. Cumhuriyet Dental Journal.2023; 26(3): 261. CrossRef - Biocompatibility, bioactive potential, porosity, and interface analysis calcium silicate repair cements in a dentin tube model
Rafaela Nanami Handa Inada, Marcela Borsatto Queiroz, Camila Soares Lopes, Evelin Carine Alves Silva, Fernanda Ferrari Esteves Torres, Guilherme Ferreira da Silva, Juliane Maria Guerreiro-Tanomaru, Paulo Sérgio Cerri, Mário Tanomaru-Filho Clinical Oral Investigations.2023; 27(7): 3839. CrossRef - A new proposal for evaluating of the solubility of bioceramic materials in dentin tubes after immersion in PBS: a laboratory investigation
Giovanna da Cunha MENDONÇA, Karina Ines Medina Carita TAVARES, Airton Oliveira SANTOS-JUNIOR, Jáder Camilo PINTO, Juliane Maria GUERREIRO-TANOMARU, Mário TANOMARU-FILHO Revista de Odontologia da UNESP.2023;[Epub] CrossRef
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Micro-computed tomographic evaluation of a new system for root canal filling using calcium silicate-based root canal sealers
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Mario Tanomaru-Filho, Fernanda Ferrari Esteves Torres, Jader Camilo Pinto, Airton Oliveira Santos-Junior, Karina Ines Medina Carita Tavares, Juliane Maria Guerreiro-Tanomaru
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Restor Dent Endod 2020;45(3):e34. Published online June 9, 2020
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DOI: https://doi.org/10.5395/rde.2020.45.e34
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Abstract
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This study evaluated by using micro-computed tomography (micro-CT) the filling ability and sealer apical extrusion promoted by a new Sealer Injection System (SIS; Angelus) with side openings needle, in comparison with the conventional injection system, associated with a new ready-to-use calcium silicate-based sealer (Bio-C Sealer). Materials and MethodsAcrylic resin models containing a main curved artificial canal and 3 simulated lateral canals in apical, middle and cervical thirds were used. The main root canals were prepared using a rotary system up to size 35.05. The canals were filled with Bio-C sealer by using a single cone technique and the conventional delivery system or SIS. Samples were scanned in micro-CT. The percentage of voids throughout the entire extension of the main root canal and in each third of the lateral canals, besides the apical extrusion of the sealer was calculated. Data were submitted to t-test (p < 0.05). ResultsThere was no difference between both systems in the main root canals filling. Although the volume percentage of voids was similar in the apical and middle thirds of lateral canals, SIS had the greatest filling ability of the cervical third lateral canal. Moreover, the conventional system showed the highest apical extrusion of the sealer. ConclusionsThe conventional and SIS obturation systems had an appropriate filling ability of the main root canal. SIS had the best filling of the cervical third of the lateral canals, besides lower sealer apical extrusion, suggesting its clinical indication.
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Citations
Citations to this article as recorded by 
- Remineralizing capacity of zinc oxide eugenol sealer following the addition of nanohydroxyapatite-tyrosine amino acid: An in vivo animal study
Rasha M. Al-Shamaa, Raghad A. Al-Askary Journal of Oral Biosciences.2025; 67(1): 100567. CrossRef - Tissues response and bone-forming capacity of zinc oxide–eugenol sealer following the addition of nanohydroxyapatite-tyrosine amino acid: An in vivo animal study
Rasha M. Al-Shamaa, Raghad A. Al-Askary Saudi Endodontic Journal.2024; 14(3): 322. CrossRef - Filling ability of ready-to-use or powder-liquid calcium silicate-based sealers after ultrasonic agitation
Mário Tanomaru-Filho, Maíra Bonassi Lucchesi, Airton Oliveira Santos-Junior, Karina Ines Medina Carita Tavares, Jáder Camilo Pinto, Juliane Maria Guerreiro-Tanomaru Brazilian Dental Journal.2024;[Epub] CrossRef - Assessment the bioactivity of zinc oxid eugenol sealer after the addition of different concentrations of nano hydroxyapatite-tyrosine amino acid
Rasha M. Al-Shamaa, Raghad A. Al-Askary Brazilian Journal of Oral Sciences.2024; 23: e243733. CrossRef - Comparative evaluation of dentinal tubule penetration and push-out bond strength of new injectable hydraulic calcium disilicate based root canal sealer: A single blinded in vitro study
Aman Verma, Anshul Arora, Sonali Taneja Journal of Oral Biology and Craniofacial Research.2024; 14(2): 143. CrossRef - A critical analysis of research methods and experimental models to study root canal fillings
Gustavo De‐Deus, Erick Miranda Souza, Emmanuel João Nogueira Leal Silva, Felipe Gonçalves Belladonna, Marco Simões‐Carvalho, Daniele Moreira Cavalcante, Marco Aurélio Versiani International Endodontic Journal.2022; 55(S2): 384. CrossRef - Micro-computed tomography in preventive and restorative dental research: A review
Mehrsima Ghavami-Lahiji, Reza Tayefeh Davalloo, Gelareh Tajziehchi, Paria Shams Imaging Science in Dentistry.2021; 51(4): 341. CrossRef - Contribution of XP‐Endo files to the root canal filling removal: A systematic review and meta‐analysis ofin vitrostudies
Emel Uzunoglu‐Özyürek, Selen Küçükkaya Eren, Sevilay Karahan Australian Endodontic Journal.2021; 47(3): 703. CrossRef - Micro‐CT evaluation of filling of flattened root canals using a new premixed ready‐to‐use calcium silicate sealer by single‐cone technique
Karina I. M. C. Tavares, Jáder C. Pinto, Airton O. Santos‐Junior, Fernanda F. E. Torres, Juliane M. Guerreiro‐Tanomaru, Mário Tanomaru‐Filho Microscopy Research and Technique.2021; 84(5): 976. CrossRef - Development of A Nano-Apatite Based Composite Sealer for Endodontic Root Canal Filling
Angelica Bertacci, Daniele Moro, Gianfranco Ulian, Giovanni Valdrè Journal of Composites Science.2021; 5(1): 30. CrossRef
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Micro-computed tomographic evaluation of the flow and filling ability of endodontic materials using different test models
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Fernanda Ferrari Esteves Torres, Juliane Maria Guerreiro-Tanomaru, Gisselle Moraima Chavez-Andrade, Jader Camilo Pinto, Fábio Luiz Camargo Villela Berbert, Mario Tanomaru-Filho
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Restor Dent Endod 2020;45(2):e11. Published online January 8, 2020
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DOI: https://doi.org/10.5395/rde.2020.45.e11
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Abstract
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This study compared the flow and filling of several retrograde filling materials using new different test models. Materials and MethodsGlass plates were manufactured with a central cavity and 4 grooves in the horizontal and vertical directions. Grooves with the dimensions used in the previous study (1 × 1 × 2 mm; length, width, and height respectively) were compared with grooves measuring 1 × 1 × 1 and 1 × 2 × 1 mm. Biodentine, intermediate restorative material (IRM), and mineral trioxide aggregate (MTA) were evaluated. Each material was placed in the central cavity, and then another glass plate and a metal weight were placed over the cement. The glass plate/material set was scanned using micro-computed tomography. Flow was calculated by linear measurements in the grooves. Central filling was calculated in the central cavity (mm3) and lateral filling was measured up to 2 mm from the central cavity. ResultsBiodentine presented the least flow and better filling than IRM when evaluated in the 1 × 1 × 2 model. In a comparison of the test models, MTA had the most flow in the 1 × 1 × 2 model. All materials had lower lateral filling when the 1 × 1 × 2 model was used. ConclusionsFlow and filling were affected by the size of the test models. Higher grooves and materials with greater flow resulted in lower filling capacity. The test model measuring 1 × 1 × 2 mm showed a better ability to differentiate among the materials.
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Citations
Citations to this article as recorded by 
- Physical, chemical and biological properties of MTA Angelus and novel AGM MTA: an in vitro analysis
Sara Nashibi, Parisa Amdjadi, SeyedehSana Ahmadi, Sara Hekmatian, Maryam Torshabi BMC Oral Health.2025;[Epub] CrossRef - Marginal Adaptation and Porosity of a Novel MTA Brand Applied as Root-End Filling Material: A Micro-CT Study
Yaneta Kouzmanova, Ivanka Dimitrova Applied Sciences.2024; 14(7): 2758. CrossRef - Evaluation of the physical properties of bromelain-modified biodentine for direct pulp capping
Paridhi Agrawal, Manoj Chandak, Aditya Patel, Jay Bhopatkar BMC Oral Health.2024;[Epub] CrossRef - How do imaging protocols affect the assessment of root-end fillings?
Fernanda Ferrari Esteves Torres, Reinhilde Jacobs, Mostafa EzEldeen, Karla de Faria-Vasconcelos, Juliane Maria Guerreiro-Tanomaru, Bernardo Camargo dos Santos, Mário Tanomaru-Filho Restorative Dentistry & Endodontics.2022;[Epub] CrossRef - A micro-computed tomographic study using a novel test model to assess the filling ability and volumetric changes of bioceramic root repair materials
Fernanda Ferrari Esteves Torres, Jader Camilo Pinto, Gabriella Oliveira Figueira, Juliane Maria Guerreiro-Tanomaru, Mario Tanomaru-Filho Restorative Dentistry & Endodontics.2021;[Epub] CrossRef - Micro-computed tomography in preventive and restorative dental research: A review
Mehrsima Ghavami-Lahiji, Reza Tayefeh Davalloo, Gelareh Tajziehchi, Paria Shams Imaging Science in Dentistry.2021; 51(4): 341. CrossRef
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