A confocal microscopic study on dentinal infiltration of one-bottle adhesive systems and self-etching priming system bonded to class V cavities

Hyung-Su Kim; Sung-Ho Park

doi:10.5395/JKACD.2002.27.3.257

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Original Article A confocal microscopic study on dentinal infiltration of one-bottle adhesive systems and self-etching priming system bonded to class V cavities: Hyung-Su Kim, Sung-Ho Park; Journal of Korean Academy of Conservative Dentistry 2002;27(3):257-269.
DOI: https://doi.org/10.5395/JKACD.2002.27.3.257
Published online: May 31, 2002

Department of Dentistry, The Graduate School of Dentistry, Yonsei University, Korea.

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Abstract
REFERENCES

Abstract

Objective
The purpose of this study was to evaluate the resin infiltration into dentin of one-bottle adhesive systems and self-etching primer bonded to Class V cavities using confocal laser scanning microscope(CLSM).
Material and Methods
Forty Class V cavities were prepared from freshly extracted caries-free human teeth. These teeth were divided into two groups based on the presence of cervical abrasion: Group I, cervical abrasion; Group II, wedge-shaped cavity preparation. Resin-dentin interfaces were produced with two one-bottle dentin bonding systems-ONE COAT BOND(OCB; Coltene®) and Syntac®Srint™(SS; VIVADENT)-, one self-etching priming system-CLEARFIL™ SE BOND(SB; KURARAY)- and one multi-step dentin bonding system-Scotchbond™Multi-Purpose(SBMP, 3M Dental Products)-as control according to manufacturers'instructions. Cavities were restored with Spectrum®(Dentsply). Specimens were immersed in saline for 24 hours and sectioned longitudinally with a low-speed diamond disc. The resin-dentin interfaces were microscopically observed using CLSM. The quality of resin-infiltrated dentin layers were evaluated by five dentists using 0-4 scale.
Results
Confocal laser scanning microscopal investigations using primer labeled with rhodamine B showed that the penetration of the primer occurred along the cavity margins.

Statistical analysis using one-way ANOVA followed by Duncan's Multiple Range test revealed that the primer penetration of the group 2(wedge-shaped cavity preparation) was more effective than group 1(cervical abrasion) and that of the gingival interfaces was more effective than the occlusal interfaces. In the one-bottle dentin bonding systems, the resin penetration score of OCB was compatible to SBMP, but those of SS and self-etching priming system, SB were lower than SBMP.

REFERENCES

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Fig. 1

Diagram of longitudinally sectioned tooth showing cavity shapes (shadowed) and schematic orientation of dentinal tubules to the class V cavity surfaces.

Fig. 2

Resin penetration scores in occlusal interfaces of class V restorations. Asterisk(*) means statistically significant differences(p<0.05)

Fig. 3

Resin penetration scores in gingival interfaces of class V restorations. Asterisk(*) means statistically significant differences(p<0.05)

Fig. 4

Comparison of resin penetrations of occlusal and gingival interfaces in group 1(cervical abrasion). Asterisk(*) means statistically significant differences (p<0.05)

Fig. 5

Comparison of resin penetrations of occlusal and gingival interfaces in group 2(V-shaped cavity preparaion). Asterisk(*) means statistically significant differences(p<0.05)

Fig. 6.1-6.5

Standard confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in occlusal interfaces of Class V cavity used for scoring the resin tag penetrations. Original magnification ×200

6.1. Score 0., 6.2. Score 1., 6.3. Score 2.,

6.4. Score 3., 6.5., Score 4.

Fig. 7.1-7.5

Standard confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of Class V cavity used for scoring the resin tag penetrations. Original magnification ×200

7.1. Score 0., 7.2. Score 1., 7.3. Score 2.,

7.4. Score 3., 7.5., Score 4.

Fig. 8

Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of Class V cavity with Scotchbond multi purpose. magnification × 800 23

Fig. 9.1-9.4

Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in occlusal interfaces of cervical abrasion. Original magnification ×200

Fig. 10.1-10.4

Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in occlusal interfaces of Class V cavity. Original magnification ×200

Fig. 11.1-11.4

Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of cervical abrasion. Original magnification ×200

Fig. 12.1-12.4

Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of Class V cavity. Original magnification ×200

Table 1

Names, numbers and characteristics of each groups of experiment

Table 2

Chemical components and instructions for use of the dentin bonding systems used in this study

Table 3

Inter-observer correlations of each groups, materials, and parameters

occlu: resin penetrations in occlusal interfaces.

gingi: resin penetrations in gingival interfaces.

Table 4

Resin penetration scores(Mean±SD) at the occlusal interfaces

Comparison among the groups: one-way ANOVA, p<0.05

Table 5

Resin penetration scores(Mean±SD) at the gingival interfaces

Tables & Figures

REFERENCES

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A confocal microscopic study on dentinal infiltration of one-bottle adhesive systems and self-etching priming system bonded to class V cavities

J Korean Acad Conserv Dent. 2002;27(3):257-269. Published online May 31, 2002

DOI: https://doi.org/10.5395/JKACD.2002.27.3.257

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Figure

A confocal microscopic study on dentinal infiltration of one-bottle adhesive systems and self-etching priming system bonded to class V cavities

Fig. 1 Diagram of longitudinally sectioned tooth showing cavity shapes (shadowed) and schematic orientation of dentinal tubules to the class V cavity surfaces.

Fig. 2 Resin penetration scores in occlusal interfaces of class V restorations. Asterisk(*) means statistically significant differences(p<0.05)

Fig. 3 Resin penetration scores in gingival interfaces of class V restorations. Asterisk(*) means statistically significant differences(p<0.05)

Fig. 4 Comparison of resin penetrations of occlusal and gingival interfaces in group 1(cervical abrasion). Asterisk(*) means statistically significant differences (p<0.05)

Fig. 5 Comparison of resin penetrations of occlusal and gingival interfaces in group 2(V-shaped cavity preparaion). Asterisk(*) means statistically significant differences(p<0.05)

Fig. 6.1-6.5 Standard confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in occlusal interfaces of Class V cavity used for scoring the resin tag penetrations. Original magnification ×200 6.1. Score 0., 6.2. Score 1., 6.3. Score 2., 6.4. Score 3., 6.5., Score 4.

Fig. 7.1-7.5 Standard confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of Class V cavity used for scoring the resin tag penetrations. Original magnification ×200 7.1. Score 0., 7.2. Score 1., 7.3. Score 2., 7.4. Score 3., 7.5., Score 4.

Fig. 8 Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of Class V cavity with Scotchbond multi purpose. magnification × 800 23

Fig. 9.1-9.4 Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in occlusal interfaces of cervical abrasion. Original magnification ×200

Fig. 10.1-10.4 Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in occlusal interfaces of Class V cavity. Original magnification ×200

Fig. 11.1-11.4 Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of cervical abrasion. Original magnification ×200

Fig. 12.1-12.4 Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of Class V cavity. Original magnification ×200

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6.1-6.5

Fig. 7.1-7.5

Fig. 8

Fig. 9.1-9.4

Fig. 10.1-10.4

Fig. 11.1-11.4

Fig. 12.1-12.4

A confocal microscopic study on dentinal infiltration of one-bottle adhesive systems and self-etching priming system bonded to class V cavities

Table 1

Names, numbers and characteristics of each groups of experiment

Table 2

Chemical components and instructions for use of the dentin bonding systems used in this study

Table 3

Inter-observer correlations of each groups, materials, and parameters

occlu: resin penetrations in occlusal interfaces.

gingi: resin penetrations in gingival interfaces.

Table 4

Resin penetration scores(Mean±SD) at the occlusal interfaces

Comparison among the groups: one-way ANOVA, p<0.05

Table 5

Resin penetration scores(Mean±SD) at the gingival interfaces

Table 1 Names, numbers and characteristics of each groups of experiment

Table 2 Chemical components and instructions for use of the dentin bonding systems used in this study

Table 3 Inter-observer correlations of each groups, materials, and parameters

occlu: resin penetrations in occlusal interfaces.

gingi: resin penetrations in gingival interfaces.

Table 4 Resin penetration scores(Mean±SD) at the occlusal interfaces

Comparison among the groups: one-way ANOVA, p<0.05

Table 5 Resin penetration scores(Mean±SD) at the gingival interfaces