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- Synergistic effect of xylitol and ursolic acid combination on oral biofilms
- Yunyun Zou, Yoon Lee, Jinyoung Huh, Jeong-Won Park
- Restor Dent Endod 2014;39(4):288-295. Published online August 27, 2014
- DOI: https://doi.org/10.5395/rde.2014.39.4.288
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Abstract
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ePub Objectives This study was designed to evaluate the synergistic antibacterial effect of xylitol and ursolic acid (UA) against oral biofilms
in vitro .Materials and Methods S. mutans UA 159 (wild type),S. mutans KCOM 1207, KCOM 1128 andS. sobrinus ATCC 33478 were used. The susceptibility ofS. mutans to UA and xylitol was evaluated using a broth microdilution method. Based on the results, combined susceptibility was evaluated using optimal inhibitory combinations (OIC), optimal bactericidal combinations (OBC), and fractional inhibitory concentrations (FIC). The anti-biofilm activity of xylitol and UA onStreptococcus spp. was evaluated by growing cells in 24-well polystyrene microtiter plates for the biofilm assay. Significant mean differences among experimental groups were determined by Fisher's Least Significant Difference (p < 0.05).Results The synergistic interactions between xylitol and UA were observed against all tested strains, showing the FICs < 1. The combined treatment of xylitol and UA inhibited the biofilm formation significantly and also prevented pH decline to critical value of 5.5 effectively. The biofilm disassembly was substantially influenced by different age of biofilm when exposed to the combined treatment of xylitol and UA. Comparing to the single strain, relatively higher concentration of xylitol and UA was needed for inhibiting and disassembling biofilm formed by a mixed culture of
S. mutans 159 andS. sobrinus 33478.Conclusions This study demonstrated that xylitol and UA, synergistic inhibitors, can be a potential agent for enhancing the antimicrobial and anti-biofilm efficacy against
S. mutans andS. sobrinus in the oral environment.-
Citations
Citations to this article as recorded by
- Therapeutic potential of ursolic acid (UA) and their derivatives with nanoformulations to combat nosocomial pathogens
Umesh Chand, Pramod Kumar Kushawaha
Future Journal of Pharmaceutical Sciences.2025;[Epub] CrossRef - Anti-cariogenic effect of experimental resin cement containing ursolic acid using dental microcosm biofilm
Jonghyun Jo, Mi-Jeong Jeon, Sun Kyu Park, Su-Jung Shin, Baek-il Kim, Jeong-Won Park
Journal of Dentistry.2024; 151: 105447. CrossRef - Alteration of oral microbial biofilms by sweeteners
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Biofilm.2024; 7: 100171. CrossRef - Synergistic inhibitory activity of Glycyrrhizae Radix and Rubi Fructus extracts on biofilm formation of Streptococcus mutans
Youngseok Ham, Tae-Jong Kim
BMC Complementary Medicine and Therapies.2023;[Epub] CrossRef - Anti-Planktonic and Anti-Biofilm Properties of Pentacyclic Triterpenes—Asiatic Acid and Ursolic Acid as Promising Antibacterial Future Pharmaceuticals
Zuzanna Sycz, Dorota Tichaczek-Goska, Dorota Wojnicz
Biomolecules.2022; 12(1): 98. CrossRef - Does Secondary Plant Metabolite Ursolic Acid Exhibit Antibacterial Activity against Uropathogenic Escherichia coli Living in Single- and Multispecies Biofilms?
Zuzanna Sycz, Dorota Wojnicz, Dorota Tichaczek-Goska
Pharmaceutics.2022; 14(8): 1691. CrossRef - The physical properties and anticariogenic effect of experimental resin cement containing ursolic acid
Hyunkyung Yoo, So Youn Kim, Su-Jung Shin, Jeong-Won Park
Odontology.2021; 109(3): 641. CrossRef - Exploration of singular and synergistic effect of xylitol and erythritol on causative agents of dental caries
Siiri Kõljalg, Imbi Smidt, Anirikh Chakrabarti, Douwina Bosscher, Reet Mändar
Scientific Reports.2020;[Epub] CrossRef - The investigation of synergistic activity of protamine with conventional antimicrobial agents against oral bacteria
Masashi Fujiki, Michiyo Honda
Biochemical and Biophysical Research Communications.2020; 523(3): 561. CrossRef - Concentration in Saliva and Antibacterial Effect of Xylitol Chewing Gum: In Vivo and In Vitro Study
Fabio Cocco, Maria Grazia Cagetti, Osama Majdub, Guglielmo Campus
Applied Sciences.2020; 10(8): 2900. CrossRef - Enhanced synergistic effects of xylitol and isothiazolones for inhibition of initial biofilm formation by Pseudomonas aeruginosa ATCC 9027 and Staphylococcus aureus ATCC 6538
Gang Zhou, Hong Peng, Ying-si Wang, Xiao-mo Huang, Xiao-bao Xie, Qing-shan Shi
Journal of Oral Science.2019; 61(2): 255. CrossRef - Alkyl rhamnosides, a series of amphiphilic materials exerting broad-spectrum anti-biofilm activity against pathogenic bacteria via multiple mechanisms
Guanghua Peng, Xucheng Hou, Wenxi Zhang, Maoyuan Song, Mengya Yin, Jiaxing Wang, Jiajia Li, Yajie Liu, Yuanyuan Zhang, Wenkai Zhou, Xinru Li, Guiling Li
Artificial Cells, Nanomedicine, and Biotechnology.2018; 46(sup3): 217. CrossRef - Ursolic acid from apple pomace and traditional plants: A valuable triterpenoid with functional properties
Simone Tasca Cargnin, Simone Baggio Gnoatto
Food Chemistry.2017; 220: 477. CrossRef - Phytochemicals for human disease: An update on plant-derived compounds antibacterial activity
Ramona Barbieri, Erika Coppo, Anna Marchese, Maria Daglia, Eduardo Sobarzo-Sánchez, Seyed Fazel Nabavi, Seyed Mohammad Nabavi
Microbiological Research.2017; 196: 44. CrossRef - Ursolic acid (UA): A metabolite with promising therapeutic potential
Dharambir Kashyap, Hardeep Singh Tuli, Anil K. Sharma
Life Sciences.2016; 146: 201. CrossRef - Experimental Models of Oral Biofilms Developed on Inert Substrates: A Review of the Literature
Lopez-Nguyen Darrene, Badet Cecile
BioMed Research International.2016; 2016: 1. CrossRef - Multi-functional Liposomes Enhancing Target and Antibacterial Immunity for Antimicrobial and Anti-Biofilm Against Methicillin-Resistant Staphylococcus aureus
Yansha Meng, Xucheng Hou, Jiongxi Lei, Mengmeng Chen, Shuangchen Cong, Yuanyuan Zhang, Weiming Ding, Guiling Li, Xinru Li
Pharmaceutical Research.2016; 33(3): 763. CrossRef - Antibacterial activity of constituents from Salvia buchananii Hedge (Lamiaceae)
Angela Bisio, Anna Maria Schito, Anita Parricchi, Giacomo Mele, Giovanni Romussi, Nicola Malafronte, Patrizia Oliva, Nunziatina De Tommasi
Phytochemistry Letters.2015; 14: 170. CrossRef
- Therapeutic potential of ursolic acid (UA) and their derivatives with nanoformulations to combat nosocomial pathogens
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- 18 Crossref
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Inhibition of
Streptococcus mutans biofilm formation on composite resins containing ursolic acid - Soohyeon Kim, Minju Song, Byoung-Duck Roh, Sung-Ho Park, Jeong-Won Park
- Restor Dent Endod 2013;38(2):65-72. Published online May 28, 2013
- DOI: https://doi.org/10.5395/rde.2013.38.2.65
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Abstract
PDFPubReaderePub
Objectives To evaluate the inhibitory effect of ursolic acid (UA)-containing composites on
Streptococcus mutans (S. mutans ) biofilm.Materials and Methods Composite resins with five different concentrations (0.04, 0.1, 0.2, 0.5, and 1.0 wt%) of UA (U6753, Sigma Aldrich) were prepared, and their flexural strengths were measured according to ISO 4049. To evaluate the effect of carbohydrate source on biofilm formation, either glucose or sucrose was used as a nutrient source, and to investigate the effect of saliva treatment, the specimen were treated with either unstimulated whole saliva or phosphate-buffered saline (PBS). For biofilm assay, composite disks were transferred to
S. mutans suspension and incubated for 24 hr. Afterwards, the specimens were rinsed with PBS and sonicated. The colony forming units (CFU) of the disrupted biofilm cultures were enumerated. For growth inhibition test, the composites were placed on a polystyrene well cluster, andS. mutans suspension was inoculated. The optical density at 600 nm (OD600) was recorded by Infinite F200 pro apparatus (TECAN). One-way ANOVA and two-way ANOVA followed by Bonferroni correction were used for the data analyses.Results The flexural strength values did not show significant difference at any concentration (
p > 0.01). In biofilm assay, the CFU score decreased as the concentration of UA increased. The influence of saliva pretreatment was conflicting. The sucrose groups exhibited higher CFU score than glucose group (p < 0.05). In bacterial growth inhibition test, all experimental groups containing UA resulted in complete inhibition.Conclusions Within the limitations of the experiments, UA included in the composite showed inhibitory effect on
S. mutans biofilm formation and growth.-
Citations
Citations to this article as recorded by- Anti-cariogenic effect of experimental resin cement containing ursolic acid using dental microcosm biofilm
Jonghyun Jo, Mi-Jeong Jeon, Sun Kyu Park, Su-Jung Shin, Baek-il Kim, Jeong-Won Park
Journal of Dentistry.2024; 151: 105447. CrossRef - Rapid specific detection of oral bacteria using Cas13-based SHERLOCK
Jett Liu, Camden Carmichael, Hatice Hasturk, Wenyuan Shi, Batbileg Bor
Journal of Oral Microbiology.2023;[Epub] CrossRef - Novel Bioactive Nanocomposites Containing Calcium Fluoride and Calcium Phosphate with Antibacterial and Low-Shrinkage-Stress Capabilities to Inhibit Dental Caries
Abdullah Alhussein, Rashed Alsahafi, Abdulrahman A. Balhaddad, Lamia Mokeem, Abraham Schneider, Mary-Ann Jabra-Rizk, Radi Masri, Gary D. Hack, Thomas W. Oates, Jirun Sun, Michael D. Weir, Hockin H. K. Xu
Bioengineering.2023; 10(9): 991. CrossRef - Quorum sensing inhibition and antibiofilm action of triterpenoids: An updated insight
Sudipta Paul Bhattacharya, Snigdha Karmakar, Kusumita Acharya, Arijit Bhattacharya
Fitoterapia.2023; 167: 105508. CrossRef - The Application of Small Molecules to the Control of Typical Species Associated With Oral Infectious Diseases
Sirui Yang, Xiaoying Lyu, Jin Zhang, Yusen Shui, Ran Yang, Xin Xu
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef - Anti-Planktonic and Anti-Biofilm Properties of Pentacyclic Triterpenes—Asiatic Acid and Ursolic Acid as Promising Antibacterial Future Pharmaceuticals
Zuzanna Sycz, Dorota Tichaczek-Goska, Dorota Wojnicz
Biomolecules.2022; 12(1): 98. CrossRef - Development and Physicochemical Characterization of Eugenia brejoensis Essential Oil-Doped Dental Adhesives with Antimicrobial Action towards Streptococcus mutans
Maury Luz Pereira, Danyelle Cristina Pereira Santos, Carlos Alberto Mendes Soares Júnior, Tamyris Alicely Xavier Nogueira Bazan, Clovis Macêdo Bezerra Filho, Márcia Vanusa da Silva, Maria Tereza dos Santos Correia, Andres Felipe Millan Cardenas, Fabiana S
Journal of Functional Biomaterials.2022; 13(3): 149. CrossRef - Does Secondary Plant Metabolite Ursolic Acid Exhibit Antibacterial Activity against Uropathogenic Escherichia coli Living in Single- and Multispecies Biofilms?
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Pharmaceutics.2022; 14(8): 1691. CrossRef - Prolonged Inhibition of Streptococcus mutans Growth and Biofilm Formation by Sustained Release of Chlorhexidine from Varnish Coated Dental Abutments: An in Vitro Study
Mark Feldman, Walid Shaaban Moustafa Elsayed, Michael Friedman, Irith Gati, Doron Steinberg, Hesham Marei, Paolo Francesco Manicone
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Annual Review of Phytopathology.2021; 59(1): 153. CrossRef - Small Molecule Compounds, A Novel Strategy against Streptococcus mutans
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Pathogens.2021; 10(12): 1540. CrossRef - Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
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Brazilian Oral Research.2021;[Epub] CrossRef - Effect of Ursolic and Oleanolic Acids on Lipid Membranes: Studies on MRSA and Models of Membranes
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Antibiotics.2021; 10(11): 1381. CrossRef - Ursolic acid inhibits multi-species biofilms developed by Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii
Xiaoying Lyu, Liang Wang, Yusen Shui, Qingsong Jiang, Lan Chen, Wen Yang, Xiaoya He, Jumei Zeng, Yuqing Li
Archives of Oral Biology.2021; 125: 105107. CrossRef - The physical properties and anticariogenic effect of experimental resin cement containing ursolic acid
Hyunkyung Yoo, So Youn Kim, Su-Jung Shin, Jeong-Won Park
Odontology.2021; 109(3): 641. CrossRef - Ursolic acid: A systematic review of its pharmacology, toxicity and rethink on its pharmacokinetics based on PK-PD model
Qiang Sun, Man He, Meng Zhang, Sha Zeng, Li Chen, Lijuan Zhou, Haibo Xu
Fitoterapia.2020; 147: 104735. CrossRef - Effects of UVB and UVC irradiation on cariogenic bacteria in vitro
Shigeki Uchinuma, Yasushi Shimada, Khairul Matin, Keiichi Hosaka, Masahiro Yoshiyama, Yasunori Sumi, Junji Tagami
Lasers in Medical Science.2019; 34(5): 981. CrossRef - Ursolic acid (UA): A metabolite with promising therapeutic potential
Dharambir Kashyap, Hardeep Singh Tuli, Anil K. Sharma
Life Sciences.2016; 146: 201. CrossRef - Protective Effects on Gastric Lesion of Ursolic acid
Sun Whoe Kim, In Young Hwang, Sun Yi Lee, Choon Sik Jeong
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- Anti-cariogenic effect of experimental resin cement containing ursolic acid using dental microcosm biofilm
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- 1 Download
- 25 Crossref
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