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- Matrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth
- Su-Jung Shin, Woocheol Lee, Jae-Il Lee, Seung-Ho Baek, Kee-Yeon Kum, Won-Jun Shon, Kwang-Shik Bae
- J Korean Acad Conserv Dent 2011;36(3):196-202. Published online May 31, 2011
- DOI: https://doi.org/10.5395/JKACD.2011.36.3.196
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Abstract
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ePub Objectives The aim of this study was to investigate levels of matrix metalloproteinase-8 (MMP-8) and substance P (SP) in root canal exudates during root canal treatment (RCT) of nonvital, painful teeth.
Materials and Methods Patients scheduled for nonsurgical RCT were prospectively selected; the study was performed after obtaining informed consent from the patients and was approved by the Institutional Review Board for Clinical Research of Gangnam Severance Hospital, Yonsei University (3-2008-0118). Canal exudates samples were collected using sterilized paper points from teeth scheduled for RCT across three different time periods. MMP-8 and SP levels were measured using enzyme-linked immunosorbent assay (ELISA). Data were analyzed using a mixed model analysis and the Pearson correlation analysis (
p < 0.05).Results MMP-8 and SP levels in GCF were decreased during RCT (
p < 0.0001), and they showed a weak positive correlation to each other (p < 0.05). Patients'subjective pain levels and the response from percussion test were significantly related to SP level.Conclusions This study demonstrated that periradicular inflammation endodontic origin can elevate SP and MMP-8 levels in root canal exudates. Interestingly, SP level of canal exudates showed a possibility of being used as an indicator of pain due to periapical pathosis.
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Original Article
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Effect of sonicated extracts of
Enterococcus faecalis on the production of matrix metalloproteinase-8 by human polymorphonuclear neutrophils - Sung-Kyung Park, Won-Jun Shon, Sung-Sam Lim
- J Korean Acad Conserv Dent 2005;30(2):138-144. Published online March 31, 2005
- DOI: https://doi.org/10.5395/JKACD.2005.30.2.138
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Abstract
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This in vitro study monitored MMP-8 production on PMN by stimulated with the following three groups; Sonicated extracts of
E. faecalis (SEF), SEF treated with Ca(OH)2 (12.5mg/ml ) for 7 days, and lipopolysaccharides (LPS) ofE. coli . The level of MMP-8 in each group was immediately measured by ELISA. The data were analyzed with Kruskal-Wallis test and Mann-Whitney U test.In the SEF group, the level of production of MMP-8 was higher than the negative control group in low concentration (0.05
µg/ml ) of SEF (p < 0.05), but it decreased with an increase in the concentration of SEF (p < 0.05). In the case of SEF treated with Ca(OH)2, all of the MMP levels were higher than negative control group (p < 0.05), but no statistical difference was found among the different SEF concentrations (p > 0.05). All of the levels inE. coli LPS were increased with increasing concentrations (p < 0.05).According to this study we could summarize as follows:
1. MMP-8 was expressed at low level in untreated PMN group and the levels of MMP-8 were upregulated in PMN stimulated by
E. coli LPS groups.2. In the SEF groups, the level of production of MMP-8 decreased with an increase in the concentration of SEF (p < 0.05). So
E. faecalis may have suppressive effect on the production of MMP-8 by PMN.3. In the case of SEF treated with Ca(OH)2, all of the MMP levels at different SEF concentrations were higher than untreated PMN group (p < 0.05), but no statistical difference was found among the different SEF concentrations (p > 0.05).
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