Restorative Dentistry & Endodontics

Research Article

Hard tissue formation after direct pulp capping with osteostatin and MTA in vivo: Ji-Hye Yoon, Sung-Hyeon Choi, Jeong-Tae Koh, Bin-Na Lee, Hoon-Sang Chang, In-Nam Hwang, Won-Mann Oh, Yun-Chan Hwang; Restor Dent Endod 2021;46(2):e17. Published online February 25, 2021; DOI: https://doi.org/10.5395/rde.2021.46.e17

Abstract PDF PubReader ePub: Objectives
In recent in vitro study, it was reported that osteostatin (OST) has an odontogenic effect and synergistic effect with mineral trioxide aggregate (MTA) in human dental pulp cells. Therefore, the aim of this study was to evaluate whether OST has a synergistic effect with MTA on hard tissue formation in vivo.
Materials and Methods
Thirty-two maxillary molars of Spraque-Dawley rats were used in this study. An occlusal cavity was prepared and the exposed pulps were randomly divided into 3 groups: group 1 (control; ProRoot MTA), group 2 (OST 100 μM + ProRoot MTA), group 3 (OST 10 mM + ProRoot MTA). Exposed pulps were capped with each material and cavities were restored with resin modified glass ionomer. The animals were sacrificed after 4 weeks. All harvested teeth were scanned with micro-computed tomography (CT). The samples were prepared and hard tissue formation was evaluated histologically. For immunohistochemical analysis, the specimens were sectioned and incubated with primary antibodies against dentin sialoprotein (DSP).
Results
In the micro-CT analysis, it is revealed that OST with ProRoot MTA groups showed more mineralized bridge than the control (p < 0.05). In the H&E staining, it is showed that more quantity of the mineralized dentin bridge was formed in the OST with ProRoot MTA group compared to the control (p < 0.05). In all groups, DSP was expressed in newly formed reparative dentin area.
Conclusions
OST can be a supplementary pulp capping material when used with MTA to make synergistic effect in hard tissue formation.

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Case Report

Oral manifestation and root canal therapy of the patient with mucopolysaccharidosis: Ji-Hye Yoon, Hyo-Il Lee, Ji-Hyun Jang, Sung-Hyeon Choi, Hoon-Sang Chang, Yun-Chan Hwang, In-Nam Hwang, Bin-Na Lee, Won-Mann Oh; Restor Dent Endod 2019;44(2):e14. Published online April 4, 2019; DOI: https://doi.org/10.5395/rde.2019.44.e14

Abstract PDF PubReader ePub: Mucopolysaccharidosis (MPS) is an inherited metabolic disorder caused by a deficiency in enzymes that participate in the degradation of glycosaminoglycans (GAGs) such as heparin sulfate and dermatan sulfate. Left untreated, patients show progressive mental and physical deterioration due to deposition of GAGs in organs. Death often occurs due to cardiac or respiratory failure before patients reach their early twenties. MPS has several oral and dental manifestations. An enlarged head, short neck, and open mouth associated with a large tongue are major characteristics of MPS patients. Dental complications can be severe, including unerupted dentition, dentigerous cyst-like follicles, malocclusions, condylar defects, and gingival hyperplasia. A 21-year-old female patient with MPS was described in this article, with special emphasis on oral manifestations and dental treatment.

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