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2 "IL-8"
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The effect of neuropeptides on secretion of Interleukin-8 (IL-8)
Kyung-Jun Kim, Sang-Hyuk Park, Kyoung-Kyu Choi, Sang-Jin Park
J Korean Acad Conserv Dent 2006;31(3):153-160.   Published online May 31, 2006
DOI: https://doi.org/10.5395/JKACD.2006.31.3.153
AbstractAbstract PDFPubReaderePub

We investigated the secretion of Interleukin-8 (IL-8) from ginviva and periodontal ligament stimulated with Substance P (SP) and Calcitonin Gene-related Peptide (CGRP). Gingiva (GF), periodontal ligament (PDLF) and pulp (PF) tissues were collected from extracted intact 3rd molars.

Cultured cells were stimulated with different concentrations of SP for 4 hrs, and stimulated with SP, CGRP and Tumor Necrosis Factor-α (TNF-α) for 8 hrs. Then RNase Protection Assay was carried out. ELISA was performed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using student t-test with significance of P < 0.05.

According to this study, the results were as follows:

IL-8 mRNA was detected in all type of cells studied (PF, GF and PDLF).

IL-8 mRNA expression was not increased after stimulating 4 hrs with SP (10-5M) and SP (10-8M) compared with Mock stimulation in all type of cells studied.

IL-8 mRNA expression was not increased after stimulating 8 hrs with SP (10-4M) and CGRP (10-6M) compared with Mock stimulation in all type of cells studied.

TNF-α(2 ng/ml) increased the expression of IL-8 mRNA in all kind of cells studied.

The secretion of IL-8 from GF was increased 8 hrs after the stimulation with CGRP (10-6M) (p < 0.05).

The secretion of IL-8 from PDLF was increased 8 hrs after the stimulation with SP (10-4M) (p < 0.05).

Calcitonin Gene-related Peptide (CGRP) increased Interleukin-8 (IL-8) which plays an important role in chemotaxis of neutrophil in Calcitonin Gene-related Peptide (CGRP) gingival tissue, whereas Substance P increased the secretion of IL-8 from periodontal ligament.

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Interleukin-8 and MCP(Monocyte Chemoattractant Protein)-1 expression by the Human Dental Pulps in cultures stimulated with Substance P
Han-Ju Shin, Sang-Hyuk Park, Gi-Woon Choi
J Korean Acad Conserv Dent 2005;30(3):193-203.   Published online May 31, 2005
DOI: https://doi.org/10.5395/JKACD.2005.30.3.193
AbstractAbstract PDFPubReaderePub

The induction of the IL-8 and MCP-1 by the stimulation of Substance P and TNF-α (IL-8 agonist) and the specificity for SP using Spantide (SP antagonist) in the dental pulp tissues was measured quantitatively. In addition, the secretion of the IL-8 in the human dental pulp tissue 36 hrs after the stimulation of SP was observed after the stimulation of SP qualitatively.

According to this study, the results were as follows:

1. There was the significant IL-8 induction at 36 h after SP (10-4M) stimulation of the pulp tissue comparing with the unstimulated dental pulp tissues (p < 0.05). IL-8 immunostaining was weakly detected along the periphery of the pulp tissue after Mock stimulation and IL-8 immunostaining was detected around the fibroblast in the pulp tissue 36h After SP (10-4M) stimulation,

2. The secretion of MCP-1 from the dental pulp tissues comparing with Mock stimulation was induced at 36 hrs after TNF-α (40 ng/ml) stimulation, but no induction with SP(10-4M). TNF-α (40 ng/ml) did not induce the IL-8 secretion from the pulp tissue, weak IL-8 immunostaining was detected along the periphery of the pulp tissue.

3. Spantide (10-5M) inhibited IL-8 induction from the pulp tissues 36 h after SP (10-4M) stimulation.

These results suggest that SP significantly induces IL-8 recruiting neutrophils in localized human dental pulp tissue. MCP-1 appears to be less involved in the early establishment of pulpal inflammation in response to irritation such as mechanical insult of dentin. SP may have positive relation with the inflammation of the human dental pulp tissues.

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