Restorative Dentistry & Endodontics

Prasanna Neelakantan

4 Articles

Effect of cryotherapy duration on experimentally induced connective tissue inflammation in vivo: Jorge Vera, Mayra Alejandra Castro-Nuñez, María Fernanda Troncoso-Cibrian, Ana Gabriela Carrillo-Varguez, Edgar Ramiro Méndez Sánchez, Viviana Sarmiento, Lourdes Lanzagorta-Rebollo, Prasanna Neelakantan, Monica Romero, Ana Arias; Restor Dent Endod 2023;48(3):e29. Published online August 2, 2023; DOI: https://doi.org/10.5395/rde.2023.48.e29

Objectives

This study tested the hypothesis that cryotherapy duration influences lipopolysaccharide (LPS)-induced inflammation in a rat model.

Materials and Methods

Six Wistar rats (Rattus norvegicus albinus) were used. Five sites were selected per animal and divided into 5 groups: a negative control group (NC), 2 positive control groups (PC1 and PC2), and 2 experimental groups (E1 and E2). Cryotherapy was applied for 1 minute (E1) or 5 minutes (E2). An acute inflammatory response was induced in the PC and E groups via subcutaneous administration of 0.5 mL/kg. In the PC2 group, a catheter was inserted without additional treatment. For the E1 and E2 groups, 2.5°C saline solution was administered through the implanted catheters for 1 and 5 minutes, respectively. The rats were sacrificed, and samples were obtained and processed for histological analysis, specifically examining the presence of polymorphonuclear neutrophils and hemorrhage. The χ² test was used to compare the presence of acute inflammation across groups. Dependent variables were compared using the linear-by-linear association test.

Results

Inflammation and hemorrhage varied significantly among the groups (p = 0.001). A significantly higher degree of acute inflammation was detected (p = 0.0002) in the PC and E1 samples than in the E2 group, in which cryotherapy was administered for 5 minutes. The PC and E1 groups also exhibited significantly greater numbers of neutrophils (p = 0.007), which were essentially absent in both the NC and E2 groups.

Conclusions

Cryotherapy administration for 5 minutes reduced the acute inflammation associated with LPS and catheter implantation.

Citations

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Determining Efficacy of Intracanal Cryotherapy on Post Endodontic Pain in Irreversible Pulpitis
Anam Fayyaz Bashir, Ussamah Waheed Jatala, Moeen ud din Ahmad, Muhammad Talha Khan, Saima Razzaq Khan, Aisha Arshad Butt
Pakistan Journal of Health Sciences.2024; : 68. CrossRef

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The effect of root canal irrigants on dentin: a focused review: Priti Pragati Rath, Cynthia Kar Yung Yiu, Jukka Pekka Matinlinna, Anil Kishen, Prasanna Neelakantan; Restor Dent Endod 2020;45(3):e39. Published online June 30, 2020; DOI: https://doi.org/10.5395/rde.2020.45.e39

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Despite the vast literature on the effects of root canal irrigants on the dentin characteristics, the precise effects of clinically relevant irrigation sequences remain unclear. In this review, we systematically dissect the role of different sequential irrigation approaches that are used in clinical endodontics. Using a systematic search strategy, we attempt to answer the question: ‘Which irrigating sequence has the most deleterious effects on dentin structure and properties?’ The effect of irrigants on the dentin composition and mechanical properties have been reviewed. A wide variety of concentrations, duration and techniques have been employed to characterize the effects of chemicals on dentin properties, thus making it impossible to draw guidelines or recommendations of irrigant sequences to be followed clinically. It was apparent that all the studied irrigation sequences potentially result in some deleterious effects on dentin such as decrease in the flexural strength, microhardness, modulus of elasticity and inorganic content and organic-inorganic ratio of the dentin. However, the literature still lacks comprehensive investigations to compare the deleterious effect of different irrigation sequences, using a wide variety of qualitative and quantitative methods. Such investigations are essential to make clinical recommendations and strategize efforts to minimize chemically-induced damage to dentin characteristics.

Citations

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Comparative Evaluation of Pulp Dissolution and Smear Layer Removal Properties of Various Herbal Extracts: An in vitro Study
Suleman Abbas Khan, Harshal Gaidhane, Saumya Navit, Meenakshi Upadhyay, Sujeet Shriram Pal, Nishi Grover
Advances in Human Biology.2025;[Epub] CrossRef
Exploring a new Portland cement-free calcium silicate cement —Part 1: Synthesis of dicalcium and tricalcium silicate
Tomomi ITOH, Kohei SHINTANI, Takashi HORIGUCHI, Norihiro SASAMOTO, Katsushi OKUYAMA, Yukimichi TAMAKI, Takeshi SUWABE, Satoshi YOKOSE, Satoshi KAWANO
Dental Materials Journal.2025;[Epub] CrossRef
Effect of phthalocyanine, methylene blue and toluidine blue photosensitizers on the adhesive interface of fiber posts: a confocal laser microscopy study
Tuba Gök, Gamze Er Karaoglu, Hulde Korucu
Lasers in Medical Science.2025;[Epub] CrossRef
Dual- or single rinse? The tubular sealer penetration of endodontic chelating agents
Beliz Ozel, Tuba Ayhan, Figen Kaptan, Fikrettin Sahin, Meriç Karapınar-Kazandağ, Ajinkya M. Pawar
PLOS ONE.2024; 19(6): e0303377. CrossRef
Mechanical properties of simulated dentin caries treated with metal cations and l-ascorbic acid 2-phosphate
Mohammad Ali Saghiri, Julia Vakhnovetsky, Amir Abdolmaleki, Elham Samadi, Fatereh Samadi, Salvatore Napoli, Michael Conte, Steven M. Morgano
Odontology.2024; 112(2): 489. CrossRef
The advancement in irrigation solution within the field of endodontics, A Review
Fatima Fahad , Raghad A Al-Hashimi , Munther J Hussain
Journal of Baghdad College of Dentistry.2024; 36(1): 54. CrossRef
Comparative evaluation of effect of modified triple antibiotic paste and calcium hydroxide as intracanal medicament on microhardness of root dentin: An in vitro study
Aparna Palekar, Piyush Mantri, Minal Awinashe, Basawaraj Biradar, Mukund Singh
Endodontology.2024;[Epub] CrossRef
Effect of herbal irrigants on surface roughness of intraradicular dentin using quantitative method of 3D surface texture analysis
Sabah M. Sobhy, Heba Abdelfatah, Hanaa M. Elgamily, Nesreen Y. Mohammed
Scientific Reports.2024;[Epub] CrossRef
Effect of different root canal irrigants on surface roughness and microhardness of Biodentine combined with triple antibiotic paste: An in vitro study
Rahul Halkai, S. Syed Ishaq, Kiran R. Halkai, Syeda Uzma Mahveen
Journal of Conservative Dentistry and Endodontics.2024; 27(5): 508. CrossRef
Bacteria debridement efficacy of two sonic root canal irrigant activation systems
Chang Zeng, Pei Hu, Colin P. Egan, Brian E. Bergeron, Franklin Tay, Jingzhi Ma
Journal of Dentistry.2024; 140: 104770. CrossRef
Effects of endodontic irrigation solutions on structural, chemical, and mechanical properties of coronal dentin: A scoping review
Joana A. Marques, Rui I. Falacho, João Miguel Santos, João Carlos Ramos, Paulo J. Palma
Journal of Esthetic and Restorative Dentistry.2024; 36(4): 606. CrossRef
Effect of ultrasonic and Er,Cr:YSGG laser-activated irrigation protocol on dual-species root canal biofilm removal: An in vitro study
Venkata Divya Durga Datla, Lakshman Varma Uppalapati, Hema Prakash Kumari Pilli, Jyothi Mandava, Sirisha Kantheti, Sri Naagaja Krishnaveni Komireddy, Vedamani Chandolu
Journal of Conservative Dentistry and Endodontics.2024; 27(6): 613. CrossRef
Comparative Evaluation of Dental Pulp Tissue Dissolution Ability of Sapindus mukorossi and Sodium Hypochlorite
Sriram Kaliamoorthy, Sreeram Rayar, Shanmugapriya SundarRaj, Sugantha Priya Sayeeram, V.V. Premkumar, Sapna C Muddappa, Venkatraman Muthukumaran, Kanmani Raju, Agila Samidorai
Cureus.2024;[Epub] CrossRef
A Comparative Evaluation of the Antimicrobial Properties of 50% Grape Seed Extract, N-acetyl Cysteine and 5.25% Sodium Hypochlorite against Enterococcus faecalis (ATCC 19433) – An In vitro Study
Nikita Vishweshwar Kurtkoti, Madhura Vivek Pawar, Vaishnavi Ketan Mathawala, Shraddha Mahadeo Shirsat
Advances in Human Biology.2024;[Epub] CrossRef
Effect of Different Irrigating Solutions on Root Canal Dentin Microhardness—A Systematic Review with Meta-Analysis
Sunidhi Agarwal, Lora Mishra, Naomi Ranjan Singh, Rini Behera, Manoj Kumar, Ravishankar Nagaraja, Krzysztof Sokolowski, Barbara Lapinska
Journal of Functional Biomaterials.2024; 15(5): 132. CrossRef
Evaluation of Effect of Herbal Irrigant on Microhardness of Root Dentin: An in vitro Study
Anuya Ravindra Koparde, Anupam Sandeep Sharma, Aniket Jadhav, Aishwarya Handa, Abhijit Bajirao Jadhav, Madhura A. Jadhav
Journal of the International Clinical Dental Research Organization.2024; 16(2): 170. CrossRef
Impact of calcium hydroxide and 2-hydroxyisocaproic acid on the microhardness of root dentine: an in vitro study
Nandini T. Niranjan, Protim Ghosh Dastidar, Raghavendra Penukonda, Galvin Sim Siang Lin, Roopa Babannavar, Arun Jaysheel, Harshada Pattar
Odontology.2024; 112(3): 711. CrossRef
Endodontic irrigants from a comprehensive perspective
Rayana Duarte Khoury, Lara Steffany de Carvalho, Mauro Felipe Rios do Nascimento, Fadi Alhussain, Amjad Abu Hasna
World Journal of Clinical Cases.2024; 12(21): 4460. CrossRef
Exploring Periostracum as an Alternative Root Canal Irrigant: Insights From Zebrafish Embryo Experiments
Annie Sylvea Valan, Jogikalmat Krithikadatta, Ajay Guru
Cureus.2024;[Epub] CrossRef
A Systematic Review of the Comparative Efficacy of Lactobacillus Probiotics and Sodium Hypochlorite as Intracanal Irrigants Against Enterococcus faecalis
Mrinalini Mrinalini, Alpa Gupta, Dax Abraham, Arun Kumar Duraisamy, Rajat Sharma
Cureus.2024;[Epub] CrossRef
Effect of sodium hypochlorite and ethylenediaminotetraacetic acid activated by laser and ultrasonic energy on surface morphology and chemical composition of intracanal dentin
Adriana Katunarić, Sandra Flinčec Grgac, Dragana Gabrić, Božidar Pavelić, Ivona Bago
Microscopy Research and Technique.2024; 87(4): 818. CrossRef
Impact of antimicrobial photodynamic therapy on the bond-strength and penetration of endodontic sealers: A systematic review
Khalid H Almadi
Photodiagnosis and Photodynamic Therapy.2023; 41: 103249. CrossRef
In Vitro Assessment of SWEEPS and Antimicrobial Photodynamic Therapy Alone or in Combination for Eradicating Enterococcus faecalis Biofilm in Root Canals
Ali Shahi Ardakani, Shima Afrasiabi, Pegah Sarraf, Stefano Benedicenti, Luca Solimei, Nasim Chiniforush
Pharmaceutics.2023; 15(11): 2628. CrossRef
Effects of traditional and novel proteolytic agents on tissue dissolution and dentine microhardness
Shwetha Elizabeth Jacob, Niharika Prasad, Sreya Dutta, Vasavi Kumblekar, Srikant Natarajan, Kukkila Jayaprakash, Manuel Sebastian Thomas
Australian Endodontic Journal.2023; 49(2): 287. CrossRef
Push-Out Bond Strength of EndoSeal Mineral Trioxide Aggregate and AH Plus Sealers after Using Three Different Irrigation Protocols
Shimaa Rifaat, Ahmed Rahoma, Fatimah Alkhalifa, Ghofran AlQuraini, Zahraa Alsalman, Zahraa Alwesaibi, Noha Taymour
European Journal of Dentistry.2023; 17(01): 076. CrossRef
Can natural irrigants replace sodium hypochlorite? A systematic review
Anand Venkatraman Susila, Shamini Sai, Nikita Sharma, Arthi Balasubramaniam, Aruna Kumari Veronica, Sureshbabu Nivedhitha
Clinical Oral Investigations.2023; 27(5): 1831. CrossRef
Advances in the Role of Sodium Hypochlorite Irrigant in Chemical Preparation of Root Canal Treatment
Chen Cai, Xuan Chen, Yang Li, Qianzhou Jiang, Yeliz Guven
BioMed Research International.2023;[Epub] CrossRef
A laboratory investigation on the effect of biguanide‐ and pyridine‐derived antiseptics on the adhesion of resin composites to dentin
Arzu Yağmur Uçar, Türkay Kölüş, D. Alperen Bozkurt, Prasanna Neelakantan, Islam A. A. Ali, Sema Belli
Australian Endodontic Journal.2023; 49(3): 599. CrossRef
Minimally invasive management of vital teeth requiring root canal therapy
E. Karatas, M. Hadis, W. M. Palin, M. R. Milward, S. A. Kuehne, J. Camilleri
Scientific Reports.2023;[Epub] CrossRef
Disinfection of radicular dentin using Riboflavin, Rose Bengal, Curcumin, and Porfimer sodium on extrusion bond strength of fiber post to radicular dentin
Sami A Alturaiki, Ahmed A. Bamanie, Mohammed A. Albulowey, Abdullah A. Al Daafas, Abdullah Almalki, Ali Alqerban
Photodiagnosis and Photodynamic Therapy.2022; 37: 102625. CrossRef
Present status and future directions: Minimally invasive root canal preparation and periradicular surgery
Prasanna Neelakantan, Vijetha Vishwanath, Silvio Taschieri, Stefano Corbella
International Endodontic Journal.2022; 55(S4): 845. CrossRef
Ex Vivo Effect of Novel Lipophosphonoxins on Root Canal Biofilm Produced by Enterococcus faecalis: Pilot Study
Yuliya Morozova, Iva Voborná, Radovan Žižka, Kateřina Bogdanová, Renata Večeřová, Dominik Rejman, Milan Kolář, Duy Dinh Do Pham, Pavel Holík, Roman Moštěk, Matej Rosa, Lenka Pospíšilová
Life.2022; 12(1): 129. CrossRef
Irrigating Solutions and Activation Methods Used in Clinical Endodontics: A Systematic Review
Riccardo Tonini, Matteo Salvadori, Elisabetta Audino, Salvatore Sauro, Maria Luisa Garo, Stefano Salgarello
Frontiers in Oral Health.2022;[Epub] CrossRef
Evaluation of Effects of Various Irrigating Solutions on Chemical Structure of Root Canal Dentin Using FTIR, SEM, and EDS: An In Vitro Study
Indu Padmakumar, Dharam Hinduja, Abdul Mujeeb, Raghu Kachenahalli Narasimhaiah, Ashwini Kumar Saraswathi, Mubashir Baig Mirza, Ali Robaian, Syed Nahid Basheer, Mohmed Isaqali Karobari, Giuseppe Alessandro Scardina
Journal of Functional Biomaterials.2022; 13(4): 197. CrossRef
Final irrigation protocols affect radicular dentin DMP1-CT expression, microhardness, and biochemical composition
Cristina Retana-Lobo, Tatiana Ramírez-Mora, Fabian Murillo-Gómez, Juliane Maria Guerreiro-Tanomaru, Mario Tanomaru-Filho, Jessie Reyes-Carmona
Clinical Oral Investigations.2022; 26(8): 5491. CrossRef
Comparative Evaluation of Antimicrobial Efficacy of Herbal Formulations of Septilin and Triphala with Conventional 2% Chlorhexidine on Root Canal and Oral Commensal Bacteria using Kirby Bauer Method
Shadab Ahmed, Kamil Shahnawaz, Tapan Kumar Mandal, Mamnoon Ghafir, Shiva Shankar Gummaluri, Gaurav Vishal
Contemporary Clinical Dentistry.2022; 13(4): 383. CrossRef
Adjunctive procedure with solvent mixtures in non-surgical endodontic retreatment: does it affect root dentin hardness?
Inês Ferreira, Ana Cristina Braga, Maria Ascensão Lopes, Irene Pina-Vaz
Odontology.2021; 109(4): 812. CrossRef

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Dentin moisture conditions strongly influence its interactions with bioactive root canal sealers: Esin Ozlek, Hüseyin Gündüz, Elif Akkol, Prasanna Neelakantan; Restor Dent Endod 2020;45(2):e24. Published online March 24, 2020; DOI: https://doi.org/10.5395/rde.2020.45.e24

Abstract PDF PubReader ePub

Objectives

It is known that bioactive materials interact with the dentin to undergo biomineralization. The exact role of moisture in this interaction is unknown. Here, we investigate the effects of dentin moisture conditions on the dislocation resistance of two bioactive root canal sealers (MTA Fillapex [Angelus Solucoes Odontologicas] and GuttaFlow BioSeal [Colténe/Whaledent AG]) at 3 weeks and 3 months after obturation.

Materials and Methods

Mandibular premolars (n = 120) were prepared and randomly divided into 3 groups based on the dentin condition: group 1, dry dentin; group 2, moist dentin; group 3, wet dentin. Each group was divided into 2 subgroups for root canal filling: MTA Fillapex and GuttaFlow BioSeal. Dislocation resistance was evaluated by measuring the push-out bond strength at 3 weeks and 3 months. Failure modes were examined under a stereomicroscope. Data were statistically analyzed by Kruskal-Wallis test with a significance level of 5%.

Results

Moist dentin resulted in higher bond strength values for both materials at both time points. This was significantly higher than wet and dry dentin for both the sealers at the 3 months (p < 0.05), while at 3 weeks it was significant only for GuttaFlow Bioseal. The different moisture conditions demonstrated similar trends in their effects on the dislocation resistance of the 2 root canal sealers.

Conclusions

The dentin moisture conditions had a significant impact on its interaction with the bioactive materials tested. Maintaining moist dentin, but not dry or wet dentin, may be advantageous before the filling root canals with bioactive sealers.

Citations

Citations to this article as recorded by

The effect of moisture conditions and canal morphologies on the filling quality of iRoot SP with single-cone technique in root canals: an ex-vivo study
Jing Yang, Xiran Xu, Jian Zhang, Kehua Que
Frontiers in Dental Medicine.2025;[Epub] CrossRef
Bond Strength and Adhesive Interface Quality of New Pre‐Mixed Bioceramic Root Canal Sealer
Gustavo Creazzo, Bruna Monteiro de Barros Ciribelli Alves, Helena Cristina de Assis, Karen Gisselle Garay Villamayor, Manoel Damião de Sousa‐Neto, Jardel Francisco Mazzi‐Chaves, Fabiane Carneiro Lopes‐Olhê
Microscopy Research and Technique.2025;[Epub] CrossRef
Evaluation of apical seal and tubular penetration of a novel bioactive glass sealer, bioceramic sealer and resin–based sealer: an In-Vitro study
M. Bilal, S. Pasha, S. Kumar, S. Arif, S. Taj, A. Saleem
Endodontics Today.2025; 23(1): 39. CrossRef
Preparation and characterization of novel nano-tricalcium silicate-58s bioactive glass-based root canal sealer
Nawal Atiya Al-Sabawi, Sawsan Hameed Al-Jubori
Saudi Endodontic Journal.2024; 14(1): 90. CrossRef
The flow behavior and sealing ability of calcium silicate root canal cement containing dimethyl sulfoxide: An in vitro study
Bokyung Shin, Ji-Hwan Seo, Wonjung Kim, Yu Jin Ahn, Ho-Young Kim, Won-Jun Shon
Journal of the Mechanical Behavior of Biomedical Materials.2024; 150: 106156. CrossRef
Nanoleakage of apical sealing using a calcium silicate-based sealer according to canal drying methods
Yoon-Joo Lee, Kyung-Mo Cho, Se-Hee Park, Yoon Lee, Jin-Woo Kim
Restorative Dentistry & Endodontics.2024;[Epub] CrossRef
Effect of operators’ proficiency level and patients’ related factors on possible complications, using a high frequency polyamide sonic intracanal irrigation device: A prospective clinical cohort study
Tobias Hahn, David W. Christofzik, Karim Fawzy El-Sayed, Sandra Freitag-Wolf, Jonas Conrad, Christian Graetz, Birte Größner-Schreiber, Christof Dörfer, Artak Heboyan
PLOS ONE.2023; 18(5): e0285492. CrossRef
Physical-chemical properties and acellular bioactivity of newly prepared nano-tricalcium silicate-58s bioactive glass-based endodontic sealer
Nawal A. Al-Sabawi, Sawsan Hameed Al-Jubori
Journal of Oral Biosciences.2023; 65(4): 305. CrossRef
Biocompatibility analysis in subcutaneous tissue and physico-chemical analysis of pre-mixed calcium silicate–based sealers
Ana Cristina Padilha Janini, Lauter Eston Pelepenko, Juliana Minto Boldieri, Victor Augusto Benedicto dos Santos, Nilvan Alves da Silva, Ivo Milton Raimundo, Brenda P. F. A. Gomes, Marina Angélica Marciano
Clinical Oral Investigations.2023; 27(5): 2221. CrossRef
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Lais Lima Pelozo, Aline Evangelista Souza-Gabriel, Guilherme Nilson Alves dos Santos, Rafael Verardino Camargo, Fabiane Carneiro Lopes-Olhê, Manoel Damião Sousa-Neto, Jardel Francisco Mazzi-Chaves
Journal of Endodontics.2023; 49(9): 1154. CrossRef
Comparison of push‐out bond strength of endodontic sealers after root canal drying with different techniques
Ahmadreza Sarrafan, Ali Soleymani, Tasnim Bagheri Chenari, Seyedali Seyedmajidi
Clinical and Experimental Dental Research.2023; 9(2): 314. CrossRef
Designing Calcium Silicate Cements with On-Demand Properties for Precision Endodontics
A. Cahyanto, P. Rath, T.X. Teo, S.S. Tong, R. Malhotra, B.N. Cavalcanti, L.Z. Lim, K.S. Min, D. Ho, W.F. Lu, V. Rosa
Journal of Dental Research.2023; 102(13): 1425. CrossRef
Outcome of root canal treatment using warm vertical compaction with bioceramic and resin‐based sealers: A randomised clinical trial
Jinghao Hu, Yunjie Zhu, Shuli Deng, Zeji Wang, Fuming He
Australian Endodontic Journal.2023; 49(S1): 170. CrossRef
Evaluation of the Sealing Ability and Bond Strength of Two Endodontic Root Canal Sealers: An In Vitro Study
Manuel Marques Ferreira, José Pedro Martinho, Inês Duarte, Diogo Mendonça, Ana Catarina Craveiro, Maria Filomena Botelho, Eunice Carrilho, Carlos Miguel Marto, Ana Coelho, Anabela Paula, Siri Paulo, Nuno Chichorro, Ana Margarida Abrantes
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How do imaging protocols affect the assessment of root-end fillings?
Fernanda Ferrari Esteves Torres, Reinhilde Jacobs, Mostafa EzEldeen, Karla de Faria-Vasconcelos, Juliane Maria Guerreiro-Tanomaru, Bernardo Camargo dos Santos, Mário Tanomaru-Filho
Restorative Dentistry & Endodontics.2022;[Epub] CrossRef
Effect of using calcium-silicate and silicone based root canal sealers in bulk or with main core material on bond strength
Gizem Kadı, Esin Özlek, Yousef Saed
Journal of Dental Research, Dental Clinics, Dental Prospects.2022; 16(4): 229. CrossRef
Physico-chemical properties of calcium silicate-based sealers in powder/liquid and ready-to-use forms
Ana C P Janini, Lauter E Pelepenko, Brenda P F A Gomes, Marina A Marciano
Brazilian Dental Journal.2022; 33(5): 18. CrossRef
Influence of dentin moisture conditions on the wetting action of different endodontic sealers using Rame-Hart goniometer: An in vitro study
Sivaji Kauravi, ShruthiH Attavar, GyanendraPratap Singh
Journal of International Oral Health.2022; 14(6): 624. CrossRef
Heating stability, physical and chemical analysis of calcium silicate‐based endodontic sealers
T. B. M. Antunes, A. C. P. Janini, L. E. Pelepenko, G. F. Abuna, E. M. Paiva, M. A. C. Sinhoreti, I. M. Raimundo, B. P. F. A. Gomes, A. de‐Jesus‐Soares, M. A. Marciano
International Endodontic Journal.2021; 54(7): 1175. CrossRef
Characterization, Antimicrobial Effects, and Cytocompatibility of a Root Canal Sealer Produced by Pozzolan Reaction between Calcium Hydroxide and Silica
Mi-Ah Kim, Vinicius Rosa, Prasanna Neelakantan, Yun-Chan Hwang, Kyung-San Min
Materials.2021; 14(11): 2863. CrossRef
Main and Accessory Canal Filling Quality of a Premixed Calcium Silicate Endodontic Sealer According to Different Obturation Techniques
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Root canal irrigants influence the hydrophobicity and adherence of Staphylococcus epidermidis to root canal dentin: an in vitro study: Venkateshbabu Nagendrababu, Omer Sheriff Sultan, Sreedharan Kannathasan, Amir Shahreza Patel, Ebenezer Chitra, Prasanna Neelakantan, Fabian Davamani; Restor Dent Endod 2018;43(1):e1. Published online December 7, 2017; DOI: https://doi.org/10.5395/rde.2018.43.e1

Abstract PDF PubReader ePub

Objectives

To determine the effect of root canal irrigants on the hydrophobicity and adherence of Staphylococcus epidermidis (S. epidermidis) to root canal dentin in vitro.

Materials and Methods

Root dentin blocks (n = 60) were randomly divided into 4 groups based on the irrigation regimen: group 1, saline; group 2, 5.25% sodium hypochlorite (NaOCl); group 3, 5.25% NaOCl followed by 17% ethylenediaminetetraacetic acid (EDTA); group 4, same as group 3 followed by 2% chlorhexidine (CHX). The hydrophobicity of S. epidermidis to root dentin was calculated by cell surface hydrophobicity while the adherence was observed by fluorescence microscopy, and bacteria were quantified using ImageJ software (National Institutes of Health). Statistical analysis of the data was done using Kruskal-Wallis test and Mann-Whitney U test (p = 0.05).

Results

The hydrophobicity and adherence of S. epidermidis to dentin were significantly increased after irrigating with group 3 (NaOCl-EDTA) (p < 0.05), whereas in group 4 (NaOCl-EDTA-CHX) both hydrophobicity and adherence were significantly reduced (p < 0.05).

Conclusions

The adherence of S. epidermidis to dentin was influenced differently by root canal irrigants. Final irrigation with CHX reduces the bacterial adherence and may impact biofilm formation.

Citations

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Quaternary ammonium silane (k21) based intracanal medicament triggers biofilm destruction
Esther Sook Kuan Kok, Xian Jin Lim, Soo Xiong Chew, Shu Fen Ong, Lok Yin See, Siao Hua Lim, Ling Ang Wong, Fabian Davamani, Venkateshbabu Nagendrababu, Amr Fawzy, Umer Daood
BMC Oral Health.2021;[Epub] CrossRef

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