We investigated the secretion of Interleukin-8 (IL-8) from ginviva and periodontal ligament stimulated with Substance P (SP) and Calcitonin Gene-related Peptide (CGRP). Gingiva (GF), periodontal ligament (PDLF) and pulp (PF) tissues were collected from extracted intact 3rd molars.
Cultured cells were stimulated with different concentrations of SP for 4 hrs, and stimulated with SP, CGRP and Tumor Necrosis Factor-α (TNF-α) for 8 hrs. Then RNase Protection Assay was carried out. ELISA was performed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using student t-test with significance of P < 0.05.
According to this study, the results were as follows:
IL-8 mRNA was detected in all type of cells studied (PF, GF and PDLF).
IL-8 mRNA expression was not increased after stimulating 4 hrs with SP (10-5M) and SP (10-8M) compared with Mock stimulation in all type of cells studied.
IL-8 mRNA expression was not increased after stimulating 8 hrs with SP (10-4M) and CGRP (10-6M) compared with Mock stimulation in all type of cells studied.
TNF-α(2 ng/ml) increased the expression of IL-8 mRNA in all kind of cells studied.
The secretion of IL-8 from GF was increased 8 hrs after the stimulation with CGRP (10-6M) (p < 0.05).
The secretion of IL-8 from PDLF was increased 8 hrs after the stimulation with SP (10-4M) (p < 0.05).
Calcitonin Gene-related Peptide (CGRP) increased Interleukin-8 (IL-8) which plays an important role in chemotaxis of neutrophil in Calcitonin Gene-related Peptide (CGRP) gingival tissue, whereas Substance P increased the secretion of IL-8 from periodontal ligament.
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