Restorative Dentistry & Endodontics

Kyung-Gyun Hwang

1 Article

Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells: Min-Seock Seo, Kyung-Gyun Hwang, Hyongbum Kim, Seung-Ho Baek; Restor Dent Endod 2012;37(3):142-148. Published online August 29, 2012; DOI: https://doi.org/10.5395/rde.2012.37.3.142

Objectives

We analyzed gene-expression profiles after 14 day odontogenic induction of human dental pulp cells (DPCs) using a DNA microarray and sought candidate genes possibly associated with mineralization.

Materials and Methods

Induced human dental pulp cells were obtained by culturing DPCs in odontogenic induction medium (OM) for 14 day. Cells exposed to normal culture medium were used as controls. Total RNA was extracted from cells and analyzed by microarray analysis and the key results were confirmed selectively by reverse-transcriptase polymerase chain reaction (RT-PCR). We also performed a gene set enrichment analysis (GSEA) of the microarray data.

Results

Six hundred and five genes among the 47,320 probes on the BeadChip differed by a factor of more than two-fold in the induced cells. Of these, 217 genes were upregulated, and 388 were down-regulated. GSEA revealed that in the induced cells, genes implicated in Apoptosis and Signaling by wingless MMTV integration (Wnt) were significantly upregulated.

Conclusions

Genes implicated in Apoptosis and Signaling by Wnt are highly connected to the differentiation of dental pulp cells into odontoblast.

Citations

Citations to this article as recorded by

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