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• In vitrocytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assay
  Sedigheh Khedmat, Somayyeh Dehghan, Jamshid Hadjati, Farimah Masoumi, Mohammad Hossein Nekoofar, Paul Michael Howell Dummer
  Restorative Dentistry & Endodontics.2014; 39(3): 149.     CrossRef
• Endodontic management of a maxillary lateral incisor with dens invaginatus and external root irregularity using cone-beam computed tomography
  Young-Jun Lim, Sook-Hyun Nam, Sung-Ho Jung, Dong-Ryul Shin, Su-Jung Shin, Kyung-San Min
  Restorative Dentistry & Endodontics.2012; 37(1): 50.     CrossRef

This study was carried out in order to determine in vitro biocompatibility of white mineral trioxide aggregate (MTA), and to compare it with that of the commonly used materials, i. e. calcium hydroxide liner (Dycal), glass ionomer cement (GIC), and Portland cement which has a similar composition of MTA. To assess the biocompatibility of each material, cytotoxicity was examined using MG-63 cells. The degree of cytotoxicity was evaluated by scanning electron microscopy (SEM) and a colorimetric method, based on reduction of the tetrazolium salt 2,3 bis {2methoxy 4nitro 5[(sulfenylamino) carbonyl] 2H tetrazolium hydroxide} (XTT) assay.

The results of SEM revealed the cells in contact with GIC, MTA, and Portland cement at 1 and 3 days were apparently healthy. In contrast, cells in the presence of Dycal appeared rounded and detached. In XTT assay, the cellular activities of the cells incubated with all the test materials except Dycal were similar, which corresponded with the SEM observation. The present study supports the view that MTA is a very biocompatible root perforation repair material. It also suggests that cellular response of Portland cement and GIC are very similar to that of MTA.

Citations

Citations to this article as recorded by  

• The effect of several root-end filling materials on MG63 osteoblast-like cells
  Jeong-Ho Lee, Won-Jun Shon, WooCheol Lee, Seung-Ho Baek
  Journal of Korean Academy of Conservative Dentistry.2010; 35(3): 222.     CrossRef
• Biocompatibility of experimental mixture of mineral trioxide aggregate and glass ionomer cement
  Min-Jae Oh, Yu-Na Jeong, In-Ho Bae, So-Young Yang, Bum-Jun Park, Jeong-Tae Koh, Yun-Chan Hwang, In-Nam Hwang, Won-Mann Oh
  Journal of Korean Academy of Conservative Dentistry.2010; 35(5): 359.     CrossRef
• Physical and chemical properties of experimental mixture of mineral trioxide aggregate and glass ionomer cement
  Yu-Na Jeong, So-Young Yang, Bum-Jun Park, Yeong-Joon Park, Yun-Chan Hwang, In-Nam Hwang, Won-Mann Oh
  Journal of Korean Academy of Conservative Dentistry.2010; 35(5): 344.     CrossRef