Citations

Citations to this article as recorded by  

• A Rare Case of Nonsyndromic Generalized Radiculomegaly with a Literature Review
  Mohammad Al-Obaida
  Case Reports in Dentistry.2020; 2020: 1.     CrossRef
• Fluoride Release and Recharge Properties of Several Fluoride-Containing Restorative Materials
  Dongyun Lee, Jongsoo Kim, Miran Han, Jisun Shin
  THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY.2020; 47(2): 196.     CrossRef
• Comparison of Microhardness and Compressive Strength of Alkasite and Conventional Restorative Materials
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The purpose of this study was to regenerate human dental pulp tissues similar to native pulp tissues. Using the mixture of type I collagen solution, primary cells collected from the different tissues (pulp, gingiva, and skin) and NIH 3T3 (1 × 10⁵ cells/ml/well) were cultured at 12-well plate at 37℃ for 14 days. Standardized photographs were taken with digital camera during 14 days and the diameter of the contracted collagen gel matrix was measured and statistically analyzed with student t-test. As one of the pulp tissue engineering, normal human dental pulp tissue and collagen gel matrix cultured with dental pulp cells for 14 days were fixed and stained with Hematoxyline & Eosin.

According to this study, the results were as follows:

1. The contraction of collagen gel matrix cultured with pulp cells for 14 days was significantly higher than other fibroblasts (gingiva, skin) (p < 0.05).

2. The diameter of collagen gel matrix cultured with pulp cells was reduced to 70.4% after 7 days, and 57.1% after 14 days.

3. The collagen gel without any cells did not contract, whereas the collagen gel cultured with gingiva and skin showed mild contraction after 14 days (88.1% and 87.6% respectively).

4. The contraction of the collagen gel cultured with NIH 3T3 cells after 14 days was higher than those cultured with gingival and skin fibroblasts, but it was not statistically significant (72.1%, p > 0.05).

5. The collagen gel matrix cultured with pulp cells for 14 days showed similar shape with native pulp tissue without blood vessels.

This approach may provide a means of engineering a variety of other oral tissue as well and these cell behaviors may provide information needed to establish pulp tissue engineering protocols.

Citations

Citations to this article as recorded by  

• Human amniotic membrane extracellular matrix scaffold for dental pulp regeneration in vitro and in vivo
  Hengameh Bakhtiar, Azin Ashoori, Sarah Rajabi, Mohamad Pezeshki‐Modaress, Alireza Ayati, Mohammad Reza Mousavi, Mohammad Reza Ellini, Amir Kamali, Amir Azarpazhooh, Anil Kishen
  International Endodontic Journal.2022; 55(4): 374.     CrossRef