KACD
Articles
- Page Path
- HOME > Restor Dent Endod > Volume 46(2); 2021 > Article
- Research Article Effect of number of uses and sterilization on the instrumented area and resistance of reciprocating instruments
-
Victor de Ornelas Peraça1
, Samantha Rodrigues Xavier1, Fabio de Almeida Gomes2, Luciane Geanini Pena dos Santos1, Erick Miranda Souza3, Fernanda Geraldo Pappen1 -
DOI: https://doi.org/10.5395/rde.2021.46.e28
Published online: April 29, 2021
1Graduation Program in Dentistry, Federal University of Pelotas, Pelotas, RS, Brazil.
2Department of Endodontics, Universidade de Fortaleza, CE, Brazil.
3Department of Endodontics, Federal University of Maranhão, São Luiz, MA, Brazil.
- Correspondence to Fernanda Geraldo Pappen, PhD. Associate Professor, Graduate Program in Dentistry, Faculty of Dentistry, Federal University of Pelotas, Gonçalves Chaves, 457/507, Pelotas, RS 96015-560 Brazil. ferpappen@yahoo.com.br
• Received: July 19, 2020 • Revised: November 28, 2020 • Accepted: December 24, 2020
Copyright © 2021. The Korean Academy of Conservative Dentistry
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
- 32 Views
- 1 Download
- 1 Web of Science
Abstract
-
Objectives This study evaluated the effect of repeated uses and autoclaving in the instrumented area, fracture resistance, and time of instrumentation of thermally treated nickel-titanium reciprocating systems.
-
Materials and Methods Two hundred simulated canals were instrumented using Reciproc Blue and WaveOne Gold. Each file was used up to 10 times or until fracture. The instrumented area was measured in pre- and post-operative images, using ImageJ software. Kaplan-Meier survival analysis evaluated the number of uses of instruments before fracture. Instrumented area and time of instrumentation were analyzed by Mann-Whitney U test and Kruskal-Wallis. Correlations among the number of uses and instrumented area were measured. The level of statistical significance was set at p < 0.05.
-
Results Reciproc Blue presented a higher estimated number of uses in comparison with WaveOne Gold (p = 0.026), but autoclaving did not affect the resistance to fracture of instruments (p > 0.05). The instrumented area was different among the evaluated groups (p = 0.039), and the instrumented area along the uses of both tested instruments was reduced. With the time of instrumentation, there was also a significant difference among the evaluated groups; the groups without sterilization cycles were faster, in comparison to those submitted to autoclaving (p = 0.010).
-
Conclusions Reciproc Blue was more resistant than WaveOne Gold, suffering later fracture. Additionally, the sterilization cycles did not influence the estimated number of uses of thermally treated reciprocating instruments, but the instrumented area of root canals was reduced along with the repeated uses of both instruments.
INTRODUCTION
The development of nickel-titanium (NiTi) instruments has enhanced root canal shaping, making it more effective, easier, and faster [1]. Despite these advantages, NiTi instruments are susceptible to deformations and/or fractures, which may affect the treatment prognosis [2]. To control these difficulties, new NiTi alloys and different cross-sectional designs have been proposed, optimizing the characteristics of endodontic instruments [3,4].
Thermal processing of NiTi alloys has improved fatigue resistance, flexibility, cutting efficiency, and canal-centering ability of instruments [4]. In addition to the thermal treatment modifications, the reciprocating motion kinematic has already been shown to extend the life span of NiTi instruments and the resistance to fatigue in comparison with continuous rotation movement [5]. Reciproc Blue (VDW, Munich, Germany), as well as WaveOne Gold (Dentsply Mailefer, Ballaigues, Switzerland) instruments, are produced from a metal alloy treated with a complex heating-cooling proper treatment, presenting improvements in the resistance and flexibility of instruments [6]. The movement of clockwise-relief and the heat treatment of the reciprocating instruments improve their resistance to fracture; however, manufacturers still recommend a single use of each instrument. Root canal preparation may damage NiTi instruments resulting in wear and deformation [7], especially because one single reciprocating instrument is responsible for the shaping of the root canal. Therefore, since these instruments are submitted to high mechanical stress load, it is possible that a decrease in cutting efficiency occurs after repeated uses, even though it is not evidenced in the literature. In addition, the sterilization cycles, essential for the re-use of the instrument, could also negatively affect its fracture resistance [8]. The re-use of reciprocating instruments is still a controversial issue and some studies advise to keep the protocol of a single-use, guaranteeing a margin of safety against the fracture of the instrument [9,10,11].
Even though Spicciarelli et al. [12] demonstrated that Reciproc files repeated use affected their shaping efficiency in simulated canals, other studies suggest that Reciproc system can be repeatedly used in root canal instrumentation, without causing anatomical deformities [9,13]. In addition, a recent clinical study demonstrated that the files of the Reciproc and WaveOne systems are safe when used in endodontic treatments of up to 3 posterior teeth [9].
Considering that there is still a lack of information regarding the resistance to fracture of thermally treated NiTi reciprocating instruments, the aim of the present study was to evaluate the effect of the number of uses and cycles of sterilization in the instrumented area and fracture resistance of 2 thermally treated NiTi reciprocating systems (WaveOne Gold e Reciproc Blue) in simulated curved canals.
MATERIALS AND METHODS
Sample size was estimated using the following parameters: medium effect size = 0.30, alpha-error = 0.05 and power beta = 95% [14]. Parameters were input into an F test family of analysis of variance (ANOVA) with fixed effects and interactions. The results indicated the need for a minimum of 195 resin blocks for performing the experiment using 4 groups and 2 different files.
Two hundred resin blocks (IM do Brasil Ltda, São Paulo, SP, Brazil) with simulated root canals with a curvature varying from 38° to 40°, 19 mm in length, initial size of ISO 15/0.02 taper were used in this study.
The resin blocks were numbered and individually photographed using a Nikon D40X digital camera, using 105 mm macro lens (Nikon Inc., Tokyo, Japan), with standardized conditions. The images were recorded in .TIFF format, with a resolution of 5,184 × 3,456 pixels. After recording the initial images, the blocks were randomly divided into 4 experimental groups (n = 50/group), according to the system to be tested and the sterilization cycles.
Reciproc Blue R25 (n = 10) and WaveOne Gold Primary (n = 10) instruments were selected to instrumentation of 2 experimental groups each. Prior to root canal preparation and after each use, each file was photographed using a Nikon D40X digital camera, with a 105 mm macro lens (Nikon Inc.), in standardized conditions at a 22 cm focal length. For each instrument, 2 registers were made: after the first picture, the instrument was rotated 180°, and photographed again. Each image was inspected for evaluation of morphological deformations or distortions that may have occurred after each use. The images were saved in .TIFF format, with a resolution of 5,184 × 3,456 pixels.
The instruments were distributed into four groups: Reciproc Blue (G1 and G2, n = 5/group) and WaveOne Gold (G3 and G4 n = 5/group). Groups 1 and 3 were composed of instruments used up to ten times and not submitted to sterilization cycles; while groups 2 and 4 were composed of instruments used up to 10 times, with cycles of sterilization between each use.
Each simulated root canal was instrumented once, and pre-instrumentation images were used as control. The canal patency was confirmed with a size 10 precurved K-file (Dentsply Maillefer, Ballaigues, Switzerland) and the working length (WL) was set 0.5 mm short of this measurement. All instruments were used in an endodontic engine (X-smart Plus; Dentsply Maillefer) at the suggested setting for each reciprocating (reciprocating “RECIPROC All” mode for Reciproc Blue instruments; reciprocating “WAVEONE gold” mode for WaveOne Gold instruments). The blocks were instrumented using the pecking motion kinematics with slight apical pressure. Each pecking depth was limited to less than 2 mm. After every 3 strokes, the debris was removed from the flutes of the NiTi file, and the artificial canal was irrigated with 2.5% sodium hypochlorite solution, followed by aspiration using the Capillary Tips system (0.48 mm and 0.35 mm, Ultradent Products Inc., Salt Lake City, UT, USA). The apical patency was maintained along the instrumentation using a size 10.02 K-file (Dentsply Maillefer). When the working length was reached, the root canal was irrigated with 2.5% sodium hypochlorite and then dried using absorbent paper points. The debris was removed from the flutes of the NiTi file, and a new sequence of photographs was achieved. These procedures were repeated until some deformation or fracture of the instruments was identified, or until the preparation of 10 simulated root canals. In groups G2 and G4, after each block instrumentation, the instruments were submitted to an autoclaving cycle. Autoclave cycles were performed at a temperature of 121°C for 30 minutes by using saturated steam under 15 psi of pressure.
For each simulated root canal, the stopwatch was triggered when the instrument had its first contact within the root canal and it was interrupted when the pre-set working length was reached.
Post-instrumentation images were obtained from the simulated root canals, using the standardized conditions used in the achievement of initial photographs. The images of each block, before and after instrumentation were saved in TIFF format and transferred to the ImageJ software (version 1.43; National Institutes of Health, Bethesda, MD, USA). Software calibration was performed using a standard image of a known measure. All the images had a standardized size, with the image of the simulated root canal centralized. The image of the root canal before and after instrumentation was selected, the number of pixels per millimeter was registered through the measure tool. The difference between pre- and postoperative values was calculated for each sample, allowing the group comparisons. Figure 1 illustrates the area measurement performed.
Figure 1
Example of a pre-instrumentation image achieved, and transferred to ImageJ software (version 1.43; National Institutes of Health). The image of the root canal before and after instrumentation was selected and the number of pixels per millimeter was registered through the measure tool.
Additionally, the images of the instruments before each use or until they showed some kind of fracture were evaluated in order to locate morphological alterations before eventual fractures (Figure 2).
Figure 2
Image of the instruments achieved to illustrate fracture and locate morphological alterations before eventual fractures. (A) WaveOne Gold with no deformation or fracture; (B) Reciproc Blue after fracture.
The results were tabulated and analyzed in IBM Statistics SPSS software version 20.0 for Windows. The number of instrumented samples before the occurrence of fracture of the instruments was analyzed using the Kaplan-Meier survival analysis.
The analysis of the data regarding the preparation time and the post-instrumentation areas resulting from the use of the different instruments was performed through the Mann Whitney and Kruskal Wallis tests since they did not present normal distribution. The Spearman correlation test was also applied, in order to evaluate the correlation between the number of uses of each instrument with the dependent variables: preparation time and prepared area of the simulated canals. The level of statistical significance was set at p < 0.05.
RESULTS
There was no evidence of morphologic alterations in the design of instruments before fracture. Reciproc Blue was able to instrument more simulated canals before fracture, in comparison to WaveOne Gold (p = 0.026). According to the Kaplan-Meier survival analysis, the sterilization cycles did not influence the estimated number of uses of Reciproc Blue (p = 0.085) and WaveOne Gold (p = 0.853). The estimated number of uses before fracture, for each evaluated group, is described in Figure 3.
Figure 3
Graphic representation of Kaplan-Meier survival analysis. The estimated survival (number of uses to fracture) of Reciproc Blue was 8.88 uses without sterilization, and 8.36 uses when autoclaved between the uses (p = 0.085); while WaveOne Gold presented an estimated survival of 7.05 without autoclaving, and 6.88 when submitted to sterilization (p = 0.853).
The instrumented area was different among the evaluated groups (p = 0.039), and the smallest area was observed in the canals instrumented with Reciproc Blue with sterilization cycles (Table 1). According to the correlation test, there was a reduction in the instrumented area along the uses of both instruments, WaveOne Gold (r = −0,731, p = 0,001 and Reciproc Blue (r = −0.923, p < 0,001).
Table 1
Instrumented area of simulated root canals (pixels per mm2), within the different evaluated groups
| Instrument | Sterilization cycle | Instrumented area | p value* |
|---|---|---|---|
| Reciproc Blue | No | 1,479.81 ± 1,130.91b | 0.039 |
| Yes | 791.75 ± 610.26a | ||
| WaveOne Gold | No | 1,144.38 ± 915.81ab | |
| Yes | 1,270.27 ± 770.59ab |
Figure 4 illustrates the reduction in the instrumented area along with the uses, using instruments with and without sterilization cycles. In relation to the time elapsed to the instrument to reach the root canal length, there was also a significant difference among the evaluated groups (p = 0.010). Data described in Table 2 demonstrate that the groups without sterilization cycles were faster, in comparison to those submitted to autoclaving.
Figure 4
Graphic illustrating the reduction in the instrumented area (pixels per mm2) along the uses within the evaluated groups (WaveOne Gold and Reciproc Blue, with and without sterilization cycles).
Table 2
Time to reach the working length with Reciproc Blue and WaveOne Gold, with and without sterilization cycles
| Instrument | Sterilization cycle | Preparation time (seg.) | p value* |
|---|---|---|---|
| Reciproc Blue | No | 109.66 ± 21.17ab | 0.010 |
| Yes | 123.21 ± 23.33b | ||
| WaveOne Gold | No | 108.89 ± 21.23a | |
| Yes | 116.35 ± 15.67ab |
DISCUSSION
In the present study, 2 thermally treated NiTi reciprocating systems were compared regarding their fracture resistance, instrumented area and time elapsed to instrumentation, as well as the effect of the number of uses and cycles of sterilization on these properties. Cyclic fatigue tests are the most important methods for endodontic instrument resistance analysis, and there are few variations applied to this test. However, the option of using standardized simulated canals, using the same file in up to ten standardized simulated canals, and after several cycles of sterilization, allows inferences closer to the clinical reality. Research involving the reuse of reciprocating files has the potential to influence the safety, effectiveness and quality of endodontic treatment, since the reuse of instruments results in the reduction of treatment cost, making instrumentation techniques more accessible.
Our main results demonstrated that the sterilization cycles did not influence the estimated number of uses of Reciproc Blue and WaveOne Gold. Nevertheless, in general, the fracture occurred later for Reciproc Blue instruments compared to WaveOne Gold instruments. The correlation test showed that there was a reduction in the instrumented area along the uses of both instruments, WaveOne Gold and Reciproc Blue.
In this study, acrylic resin simulated canals were used due to the need for anatomic standardization, which could hardly be obtained if natural teeth were used. Although simulated canals are widely used in studies related to root canal instrumentation [15,16,17,18] this method has limitations. These limitations are mainly related to the difference in microhardness of the resin and dentin, and to the effect of heat generation from the movement of the instruments in these canals, which favors the cutting of the material [19,20]. Due to these structural differences between resin specimens and dentin walls, the results of the present study should be interpreted with caution.
According to the manufacturers, WaveOne Gold and Reciproc Blue files are designed to be used in only one clinical case. The manufacturers alert to the risks that the reuse of files could cause in the resistance of the instruments. In addition, it has been demonstrated the mechanical effects of the reuse of reciprocating instruments [21]. In response to these recommendations, recent studies have indicated that the use of these instruments in multiple cases can be safe and efficient [9,22].
Even though there have been suggestions that the files manufactured from M-Wire alloy are not influenced by the sterilization process [5,23], this is the first study evaluating the effect of the sterilization process and the multiple uses of second generation reciprocating instruments. In agreement with previous results [23], where the cyclic fatigue resistance of conventional Reciproc files was not affected by up to 10 sterilization cycles, our study showed that sterilization did not affect the estimated number of canals instrumented by Reciproc Blue system and WaveOne Gold. However, in general, the fracture occurred later for Reciproc Blue instruments compared to WaveOne Gold instruments. This result can be explained by the higher torsional strength of Reciproc Blue files in relation to WaveOne Gold files [6,24].
Several producers have developed special thermomechanical treatments in order to produce NiTi alloys, which mainly contain a stable martensitic phase under clinical conditions. Thus, numerous thermomechanical treatments have been suggested, such as the Control Memory (CM; Coltene, Cuyahoga Falls, OH, USA), the Blue Technology (Dentsply Tulsa Dental Specialties, Tulsa, OK, USA), and the Gold Technology (Dentsply Tulsa Dental Specialties). The present results demonstrate a high frequency of fracture of thermally treated instruments after repeated uses, with or without cycles of sterilization. Even though NiTi properties are usually reported as extremely influenced by thermomechanical processing, the effects of heat retreatment achieved during autoclaving procedures did not significantly affect the cyclic fatigue strength of NiTi instruments [14,25,26,27].
Regarding the instrumented area, after repeated uses, WaveOne Gold and Reciproc Blue instruments resulted in diminished instrumented area. Our results are in agreement with those reported by Kowalczuck et al. [28] who demonstrated the loss of cutting ability of Reciproc R25 file, and significant reduction of the instrumented area within the repeated uses. Nevertheless, the present study is the first one to evaluate the instrumented area achieved after repeated uses of Reciproc Blue and WaveOne Gold files taking into account the sterilization cycles. Our study also demonstrated that the smallest instrumented area was observed in the canals instrumented with Reciproc Blue with sterilization cycles, however, comparison between instruments should take into account that instruments with different tip taper and design will reflect in the different bidimensional image of preparation. NiTi properties are usually reported as extremely influenced by thermomechanical processing, consequently, the heat retreatment achieved during autoclaving procedures could significantly affect the cyclic fatigue strength of NiTi instruments, or the cutting ability of these instruments. This hypothesis was then, tested in the present study. Our results demonstrated, as described in Table 1, that WaveOne Gold had similar instrumented area in both groups, with or without sterilization cycles, demonstrating that it was not negatively affected by thermal changes during autoclaving. Reciproc Blue, however, showed less instrumented area after the same cycles.
The instrumentation time is dependent on several factors such as the applied technique and the operator's experience [29]. In this study, this adversity was overcome since a single operator instrumented all the canals. The literature had shown that the conventional Reciproc system produced significantly faster root canal preparation than WaveOne Gold [30]. However, Bürklein et al. [31] demonstrated that the preparation time using Reciproc Blue and WaveOne Gold are similar; and this statement was confirmed by our results. Nevertheless, the present study showed that the sterilization cycles seemed to interfere in the ability of instruments to reach the root canal length, probably because the heat achieved during autoclaving may diminish the cutting capacity of instruments.
CONCLUSIONS
In conclusion, our results demonstrated that Reciproc Blue was more resistant than WaveOne Gold, suffering later fracture. Additionally, the sterilization cycles did not influence the estimated number of uses of thermally treated reciprocating instruments, but the instrumented area of root canals was reduced along with the repeated uses of both instruments.
ACKNOWLEDGEMENTS
The research received financial support from CNPq Grant – PIBIC (V.O.P.).
-
Conflict of Interest: No potential conflict of interest relevant to this article was reported.
-
Author Contributions:
Conceptualization: Pappen FG, Souza EM.
Data curation: Xavier SR, Peraça VO.
Formal analysis: Pappen FG, Souza EM.
Funding acquisition: Souza EM, Peraça VO.
Investigation: Xavier SR, Santos LGP.
Methodology: Santos LGP, Almeida Gomes F.
Project administration: Pappen FG, Santos LGP.
Supervision: Pappen FG.
Writing - original draft: Peraça VO, Santos LGP, Xavier SR.
Writing - review & editing: Pappen FG, Almeida Gomes F, Souza EM.
ARTICLE INFORMATION
- 1. Hasheminia SM, Farhad A, Sheikhi M, Soltani P, Hendi SS, Ahmadi M. Cone-beam computed tomographic analysis of canal transportation and centering ability of single-file systems. J Endod 2018;44:1788-1791. PubMed
- 2. Abu-Tahun IH, Kwak SW, Ha JH, Kim HC. Microscopic features of fractured fragment of nickel-titanium endodontic instruments by two different modes of torsional loading. Scanning 2018;2018:9467059. ArticlePubMedPMCPDF
- 3. Es-Souni M, Es-Souni M, Brandies HF. On the transformation behaviour, mechanical properties and biocompatibility of two NiTi-based shape memory alloys: NiTi42 and NiTi42Cu7. Biomaterials 2001;22:2153-2161. ArticlePubMed
- 4. Zupanc J, Vahdat-Pajouh N, Schäfer E. New thermomechanically treated NiTi alloys - a review. Int Endod J 2018;51:1088-1103. ArticlePubMedPDF
- 5. Kiefner P, Ban M, De-Deus G. Is the reciprocating movement per se able to improve the cyclic fatigue resistance of instruments? Int Endod J 2014;47:430-436. ArticlePubMed
- 6. Silva EJNL, Vieira VTL, Hecksher F, Dos Santos Oliveira MRS, Dos Santos Antunes H, Moreira EJL. Cyclic fatigue using severely curved canals and torsional resistance of thermally treated reciprocating instruments. Clin Oral Investig 2018;22:2633-2638.ArticlePubMedPDF
- 7. Yamazaki-Arasaki A, Cabrales R, Santos Md, Kleine B, Prokopowitsch I. Topography of four different endodontic rotary systems, before and after being used for the 12th time. Microsc Res Tech 2012;75:97-102. ArticlePubMed
- 8. Silva EJNL, Zanon M, Hecksher F, Belladonna FG, de Vasconcelos RA, Fidalgo TKDS. Influence of autoclave sterilization procedures on the cyclic fatigue resistance of heat-treated nickel-titanium instruments: a systematic review. Restor Dent Endod 2020;45:e25. ArticlePubMedPMCPDF
- 9. Bueno CSP, Oliveira DP, Pelegrine RA, Fontana CE, Rocha DGP, Bueno CEDS. Fracture incidence of WaveOne and Reciproc files during root canal preparation of up to 3 posterior teeth: a prospective clinical study. J Endod 2017;43:705-708. PubMed
- 10. Bueno CSP, Oliveira DP, Pelegrine RA, Fontana CE, Rocha DGP, Gutmann JL, Bueno CES. Fracture incidence of WaveOne Gold files: a prospective clinical study. Int Endod J 2020;53:1192-1198. ArticlePubMedPDF
- 11. Duque JA, Bramante CM, Duarte MAH, Alcalde MP, Silva EJNL, Vivan RR. Cyclic fatigue resistance of nickel-titanium reciprocating instruments after simulated clinical use. J Endod 2020 8 18 . [Epub ahead of print].Article
- 12. Spicciarelli V, Corsentino G, Ounsi HF, Ferrari M, Grandini S. Shaping effectiveness and surface topography of reciprocating files after multiple simulated uses. J Oral Sci 2019;61:45-52. ArticlePubMed
- 13. Barros SGO, de Lima CO, Vieira VTL, Neves AA, Accorsi-Mendonça T, da Silva EJNL. Bending resistance and quantitative transportation assessment after multiple uses of a reciprocating instrument. Endod Pract Today 2018;12:251-256.
- 14. Özyürek T, Yılmaz K, Uslu G. The effects of autoclave sterilization on the cyclic fatigue resistance of ProTaper Universal, ProTaper Next, and ProTaper Gold nickel-titanium instruments. Restor Dent Endod 2017;42:301-308. ArticlePubMedPMCPDF
- 15. Di Nardo D, Miccoli G, Mazzoni A, Seracchiani M, Gambarini G, Testarelli L. Centering ability of a new nickel-titanium rotary instruments with a peculiar flat-side design: an in vitro study. J Contemp Dent Pract 2020;21:539-542. PubMed
- 16. Campanella V, Gianni L, Libonati A, Gallusi G. Shaping ability of Reciproc R25 file and Mtwo system used in continuous and reciprocating motion. J Contemp Dent Pract 2020;21:171-177. ArticlePubMed
- 17. Alfadley A, Alrajhi A, Alissa H, Alzeghaibi F, Hamadah L, Alfouzan K, Jamleh A. Shaping ability of XP Endo Shaper file in curved root canal models. Int J Dent 2020;2020:4687045. PubMedPMC
- 18. Silva EJ, Tameirão MD, Belladonna FG, Neves AA, Souza EM, De-Deus G. Quantitative transportation assessment in simulated curved canals prepared with an adaptive movement system. J Endod 2015;41:1125-1129. ArticlePubMed
- 19. Coleman CL, Svec TA. Analysis of Ni-Ti versus stainless steel instrumentation in resin simulated canals. J Endod 1997;23:232-235. ArticlePubMed
- 20. You SY, Kim HC, Bae KS, Baek SH, Kum KY, Lee W. Shaping ability of reciprocating motion in curved root canals: a comparative study with micro-computed tomography. J Endod 2011;37:1296-1300. PubMed
- 21. Gavini G, Caldeira CL, Akisue E, Candeiro GT, Kawakami DA. Resistance to flexural fatigue of Reciproc R25 files under continuous rotation and reciprocating movement. J Endod 2012;38:684-687. ArticlePubMed
- 22. Pirani C, Paolucci A, Ruggeri O, Bossù M, Polimeni A, Gatto MR, Gandolfi MG, Prati C. Wear and metallographic analysis of WaveOne and reciproc NiTi instruments before and after three uses in root canals. Scanning 2014;36:517-525. ArticlePubMedPDF
- 23. Cunha RS, Junaid A, Ensinas P, Nudera W, Bueno CE. Assessment of the separation incidence of reciprocating WaveOne files: a prospective clinical study. J Endod 2014;40:922-924. ArticlePubMed
- 24. Özyürek T, Gündoğar M, Uslu G, Yılmaz K, Staffoli S, Nm G, Plotino G, Polimeni A. Cyclic fatigue resistances of Hyflex EDM, WaveOne gold, Reciproc blue and 2shape NiTi rotary files in different artificial canals. Odontology 2018;106:408-413. PubMed
- 25. Plotino G, Costanzo A, Grande NM, Petrovic R, Testarelli L, Gambarini G. Experimental evaluation on the influence of autoclave sterilization on the cyclic fatigue of new nickel-titanium rotary instruments. J Endod 2012;38:222-225. PubMed
- 26. Zhao D, Shen Y, Peng B, Haapasalo M. Effect of autoclave sterilization on the cyclic fatigue resistance of thermally treated nickel-titanium instruments. Int Endod J 2016;49:990-995. ArticlePubMed
- 27. Champa C, Divya V, Srirekha A, Karale R, Shetty A, Sadashiva P. An analysis of cyclic fatigue resistance of reciprocating instruments in different canal curvatures after immersion in sodium hypochlorite and autoclaving: an in vitro study. J Conserv Dent 2017;20:194-198. ArticlePubMedPMC
- 28. Kowalczuck A, Sydney GB, Martinez EF, Cardoso RJ. Evaluation of cutting ability and plastic deformation of reciprocating files. Braz Oral Res 2016;30:1-6.
- 29. Lim KC, Webber J. The validity of simulated root canals for the investigation of the prepared root canal shape. Int Endod J 1985;18:240-246. PubMed
- 30. Saber SEDM, Nagy MM, Schäfer E. Comparative evaluation of the shaping ability of WaveOne, Reciproc and OneShape single-file systems in severely curved root canals of extracted teeth. Int Endod J 2015;48:109-114. PubMed
- 31. Bürklein S, Flüch S, Schäfer E. Shaping ability of reciprocating single-file systems in severely curved canals: WaveOne and Reciproc versus WaveOne Gold and Reciproc blue. Odontology 2019;107:96-102. ArticlePubMedPDF
REFERENCES
Tables & Figures
REFERENCES
Citations
Citations to this article as recorded by 
ePub Link
Cite
XML DownloadEffect of number of uses and sterilization on the instrumented area and resistance of reciprocating instruments
Figure 1 Example of a pre-instrumentation image achieved, and transferred to ImageJ software (version 1.43; National Institutes of Health). The image of the root canal before and after instrumentation was selected and the number of pixels per millimeter was registered through the measure tool.
Figure 2 Image of the instruments achieved to illustrate fracture and locate morphological alterations before eventual fractures. (A) WaveOne Gold with no deformation or fracture; (B) Reciproc Blue after fracture.
Figure 3 Graphic representation of Kaplan-Meier survival analysis. The estimated survival (number of uses to fracture) of Reciproc Blue was 8.88 uses without sterilization, and 8.36 uses when autoclaved between the uses (p = 0.085); while WaveOne Gold presented an estimated survival of 7.05 without autoclaving, and 6.88 when submitted to sterilization (p = 0.853).
Figure 4 Graphic illustrating the reduction in the instrumented area (pixels per mm2) along the uses within the evaluated groups (WaveOne Gold and Reciproc Blue, with and without sterilization cycles).
Figure 1
Figure 2
Figure 3
Figure 4
Effect of number of uses and sterilization on the instrumented area and resistance of reciprocating instruments
Instrumented area of simulated root canals (pixels per mm2), within the different evaluated groups
| Instrument | Sterilization cycle | Instrumented area | p value* |
|---|---|---|---|
| Reciproc Blue | No | 1,479.81 ± 1,130.91b | 0.039 |
| Yes | 791.75 ± 610.26a | ||
| WaveOne Gold | No | 1,144.38 ± 915.81ab | |
| Yes | 1,270.27 ± 770.59ab |
Values are expressed as mean ± standard deviation.
a,bDifferent superscript letters indicate statistically significant differences. *Kruskal-Wallis test.
Time to reach the working length with Reciproc Blue and WaveOne Gold, with and without sterilization cycles
| Instrument | Sterilization cycle | Preparation time (seg.) | p value* |
|---|---|---|---|
| Reciproc Blue | No | 109.66 ± 21.17ab | 0.010 |
| Yes | 123.21 ± 23.33b | ||
| WaveOne Gold | No | 108.89 ± 21.23a | |
| Yes | 116.35 ± 15.67ab |
Values are expressed as mean ± standard deviation.
a,bDifferent superscript letters indicate statistically significant differences. *Kruskal-Wallis test.
Table 1 Instrumented area of simulated root canals (pixels per mm2), within the different evaluated groups
Values are expressed as mean ± standard deviation.
a,bDifferent superscript letters indicate statistically significant differences. *Kruskal-Wallis test.
Table 2 Time to reach the working length with Reciproc Blue and WaveOne Gold, with and without sterilization cycles
Values are expressed as mean ± standard deviation.
a,bDifferent superscript letters indicate statistically significant differences. *Kruskal-Wallis test.
