Effect of a rewetting agent on dentin adhesion

Young-Gon Cho; Yil-Yoon Park; Young-Gon Lee

doi:10.5395/JKACD.2003.28.1.011

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Original Article Effect of a rewetting agent on dentin adhesion: Young-Gon Cho, Yil-Yoon Park, Young-Gon Lee; Journal of Korean Academy of Conservative Dentistry 2003;28(1):11-22.
DOI: https://doi.org/10.5395/JKACD.2003.28.1.011
Published online: January 31, 2003

Department of Conservative Dentistry, College of Dentistry, Chosun University, Korea.

Corresponding author (ygcho@mail.chosun.ac.kr)

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Abstract
REFERENCES

Abstract

This study compared the dentin adhesion to composite resin according to air-dry, blot dry, application of rewetting agent on air-dry or blot dry dentin surface by microleakag test and SEM observation.

For microleakage test, class V cavity preparations with dentinal margins were prepared on both buccal and lingual surfaces of 40 extracted human molars. For SEM observation, occlusal dentin of 20 extracted human molars were exposed.

After etched the dentin, prepared teeth were randomly divided into four groups;

D group: air dry for 10-15 sec., B group: blot dry with moist cotton pellet, D-R group: air dry and rewet with Aqua-Prep F for 20 sec., B-R group: blot dry and rewet with Aqua-Prep F for 20 sec.

Treated cavities and surfaces were filled or constructed using One-Step adhesives and Aelitefil composite resins. Specimens were stored in distilled water for 24 hours. For microleakage test, the specimens were thermocycled and soaked into 2% methylene blue. The specimens were sectioned longitudinally and evaluated for microleakage under steromicroscope. The data were statistically analysed by Kruskal-Wallis Test, Mann-Whitney and Wilcoxon signed ranked tests. For SEM observation, the specimens were bisectioned mesiodiatally. After decalcified and deproteinized, specimens were observed under SEM.

The results of this study were as follows;

1. The microleakges on dentinal margin were the highest in D group compared with B group, D-R group, and B-R group(p<0.05). But there was no significant difference between B group, D-R group and B-R group.

2. D group showed gap and a few resin tags between dentin and composite resin.

3. B group, D-R group, and B-R groups showed close adaptation between dentin and composite resin. It showed that resin rags in B group were numerous and long, in D-R group were few and short, in B-R group were numerous and short or long.

4. Adhesive layer showed in D-R group (10 µm) and B-R group (3 µm)

In conclusion, use of rewetting agent to dry dentin was efficient to dentin adhesion, also it did not provide reverse effect on blot dry dentin.
Keywords: Dentin adhesion; Air-dry; Blot dry; Rewetting agent; Microleakage; SEM

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Fig. 1

Numbers of leakage scores of each group at dentin margin

Fig. 2

Resin-dentin interface produced using One-Step on dry dentin (D group). There is 10 µm thick gap (g) between composite (C) and dentin (D) and no discernible hybrid layer. (SEM×500)

Fig. 3

Higher magnification of D group (SEM×2,000). A few and short resin tags (t) of about 3-4 µm length protruded from composite (C) showed.

Fig. 4

Resin-dentin interface produced using One-Step on blot dry dentin (B group). Resin (C)-dentin (D) interface showed close adaptation and hybrid layer (h). A numerous 10-40 µm long resin tags showed from hybrid layer to dentin. (SEM×500)

Fig. 5

Higher magnification of B group (SEM×2,000). Hybrid layer (h) and funnel shaped resin tags (t) showed between composite (C) and dentin (D).

Fig. 6

Resin-dentin interface produced using One-Step on dry dentin rewetted with Aqua-Prep F (D-R group). Resin (C)-dentin (D) interface showed close adaptation and hybrid layer. Adhesive layer (a) of 10 µm thickness showed between composite (C) and hybrid layer (h). (SEM×500)

Fig. 7

Higher magnification of D-R group (SEM×2,000). Adhesive layer (a), short (about 10 µm long) and less profound resin tags (t), and hybrid layer (h) showed.

Fig. 8

Resin-dentin interface produced using One-Step on blot dry dentin rewetted with Aqua-Prep F (B-R group). Resin-dentin interface showed close adaptation and hybrid layer. Adhesive layer (a) of 3 µm thickness showed between composite (C) and hybrid layer (h). A numerous 10-40 µm long resin tags showed from hybrid layer to dentin (D). (SEM×500)

Fig. 9

Higher magnification of B-R group (SEM×2,000). Short and long resin tags (about 10-40 µm long) showed from hybrid layer (h) to dentin (D).

Table 1

Materials used in this study and chemical composition

Table 2

Group classification according to dry, blot dry, and rewetting procedures of dentin surface

Table 3

Distribution of microleakage scores and means at dentin margin

Table 4

Statistical analysis of microleakage at dentin margin between each group by Kruskal-Wallis test and Mann-Whitney test

^*: significant differences (p<0.05)

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REFERENCES

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Effect of a rewetting agent on dentin adhesion

J Korean Acad Conserv Dent. 2003;28(1):11-22. Published online January 31, 2003

DOI: https://doi.org/10.5395/JKACD.2003.28.1.011

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Effect of a rewetting agent on dentin adhesion

Fig. 1 Numbers of leakage scores of each group at dentin margin

Fig. 2 Resin-dentin interface produced using One-Step on dry dentin (D group). There is 10 µm thick gap (g) between composite (C) and dentin (D) and no discernible hybrid layer. (SEM×500)

Fig. 3 Higher magnification of D group (SEM×2,000). A few and short resin tags (t) of about 3-4 µm length protruded from composite (C) showed.

Fig. 4 Resin-dentin interface produced using One-Step on blot dry dentin (B group). Resin (C)-dentin (D) interface showed close adaptation and hybrid layer (h). A numerous 10-40 µm long resin tags showed from hybrid layer to dentin. (SEM×500)

Fig. 5 Higher magnification of B group (SEM×2,000). Hybrid layer (h) and funnel shaped resin tags (t) showed between composite (C) and dentin (D).

Fig. 6 Resin-dentin interface produced using One-Step on dry dentin rewetted with Aqua-Prep F (D-R group). Resin (C)-dentin (D) interface showed close adaptation and hybrid layer. Adhesive layer (a) of 10 µm thickness showed between composite (C) and hybrid layer (h). (SEM×500)

Fig. 7 Higher magnification of D-R group (SEM×2,000). Adhesive layer (a), short (about 10 µm long) and less profound resin tags (t), and hybrid layer (h) showed.

Fig. 8 Resin-dentin interface produced using One-Step on blot dry dentin rewetted with Aqua-Prep F (B-R group). Resin-dentin interface showed close adaptation and hybrid layer. Adhesive layer (a) of 3 µm thickness showed between composite (C) and hybrid layer (h). A numerous 10-40 µm long resin tags showed from hybrid layer to dentin (D). (SEM×500)

Fig. 9 Higher magnification of B-R group (SEM×2,000). Short and long resin tags (about 10-40 µm long) showed from hybrid layer (h) to dentin (D).

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Effect of a rewetting agent on dentin adhesion

Table 1

Materials used in this study and chemical composition

Table 2

Group classification according to dry, blot dry, and rewetting procedures of dentin surface

Table 3

Distribution of microleakage scores and means at dentin margin

Table 4

Statistical analysis of microleakage at dentin margin between each group by Kruskal-Wallis test and Mann-Whitney test

^*: significant differences (p<0.05)

Table 1 Materials used in this study and chemical composition

Table 2 Group classification according to dry, blot dry, and rewetting procedures of dentin surface

Table 3 Distribution of microleakage scores and means at dentin margin

Table 4 Statistical analysis of microleakage at dentin margin between each group by Kruskal-Wallis test and Mann-Whitney test