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The remineralization aspect of enamel according to change of the degree of saturation of the organic acid buffering solution in pH 5.5
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Basic Research The remineralization aspect of enamel according to change of the degree of saturation of the organic acid buffering solution in pH 5.5
Jin-Sung Park, Sung-Ho Park, Jeong-Won Park, Chan-Young Lee
Journal of Korean Academy of Conservative Dentistry 2010;35(2):96-105.
DOI: https://doi.org/10.5395/JKACD.2010.35.2.096
Published online: March 31, 2010

Department of Dentistry, the Graduate School, Yonsei University, Seoul, Korea.

Corresponding Author: Chan-Young Lee. Department of Conservative Dentistry College of Dentistry, Yonsei University, 134 Shinchon-dong, Seodaemun-ku, Seoul, 120-752, Korea. Tel: 82-2-2228-8700, Fax: 82-2-313-7575, chanyoungl@yuhs.ac
• Received: December 28, 2009   • Revised: January 17, 2010   • Accepted: March 11, 2010

Copyright © 2010 Korean Academy of Conservative Dentistry

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  • The purpose of this study is to observe and compare the remineralization tendencies of artificial enamel caries lesion by remineralization solutions of different degree of saturations at pH 5.5, using a polarizing microscope and computer programs (Photoshop, Image pro plus, Scion Image, Excel).
    For this study, 48 sound permanent teeth with no signs of demineralization, cracks, or dental restorations were used. The specimens were immersed in lactic acid demineralization solution for 2 days in order to produce artificial dental caries that consist of surface and subsurface lesions. Each of 9 or 10 specimens was immersed in pH 5.5 lactic acid buffering remineralization solution of four different degrees of saturation (0.507, 0.394, 0.301, and 0.251) for 12 days. After the demineralization and remineralization, images were taken by a polarizing microscope (×100). The results were obtained by observing images of the specimens, and using computer programs, the density of caries lesions were estimated.
    While the group with the lowest degree of saturation (0.251) showed total remineralization feature from the surface to the subsurface of the lesion, the group with the highest degree of saturation (0.507) showed demineralization mainly on the surface of the lesion at the constant organic acid concentration 0.01 M and pH 5.5.
  • Keywords: Demineralization; Remineralization; pH; Degree of saturation; Artificial cares; Enamel
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Figure 1
Polarizing microscopic observation of demineralized enamel (Group 1, × 100).
jkacd-35-96-g001.jpg
Figure 2
Polarizing microscopic observation of remineralized enamel (Group 1, × 100).
jkacd-35-96-g002.jpg
Figure 3
Polarizing microscopic observation of demineralized enamel (Group 2, × 100).
jkacd-35-96-g003.jpg
Figure 4
Polarizing microscopic observation of remineralized enamel (Group 2, × 100).
jkacd-35-96-g004.jpg
Figure 5
Polarizing microscopic observation of demineralized enamel (Group 3, × 100).
jkacd-35-96-g005.jpg
Figure 6
Polarizing microscopic observation of remineralized enamel (Group 3, × 100).
jkacd-35-96-g006.jpg
Figure 7
Polarizing microscopic observation of demineralized enamel (Group 4, × 100).
jkacd-35-96-g007.jpg
Figure 8
Polarizing microscopic observation of remineralized enamel (Group 4, × 100).
jkacd-35-96-g008.jpg
Figure 9
Change ratio of demineralized depth
(demineralized depth after remineralization / demineralized depth before remineralization) × 100 (%).
jkacd-35-96-g009.jpg
Figure 10
Change rate of surface lesion width
(surface lesion width after remineralization / surface lesion width before remineralization) × 100 (%).
jkacd-35-96-g010.jpg
Figure 11
Comparison of density in enamel area before and after remineralization (Group 1).
jkacd-35-96-g011.jpg
Figure 12
Comparison of density in enamel area before and after remineralization (Group 2).
jkacd-35-96-g012.jpg
Figure 13
Comparison of density in enamel area before and after remineralization (Group 3).
jkacd-35-96-g013.jpg
Figure 14
Comparison of density in enamel area before and after remineralization (Group 4).
jkacd-35-96-g014.jpg
Figure 15
Change rate of remineralizalized amount before and after remineralization ((demineralized area before remineralization-demineralized area after remineralization/demineralized area before remineralization) ×100 + 100 (%)).
jkacd-35-96-g015.jpg
Table 1
Initial composition of demineralization solution
jkacd-35-96-i001.jpg
Table 2
Initial composition of remineralization solution
jkacd-35-96-i002.jpg
Table 3
One-way ANOVA on the remineralizing features of pH 5.5 solutions of different degree of saturations on artificially demineralized enamel

* p < .05

jkacd-35-96-i003.jpg
Table 4
Demineralization depth (mm) and value change (%) during de- and remineralization

Standard Deviation(SD) is in the parentheses.

jkacd-35-96-i004.jpg
Table 5
Surface lesion width (mm) and value change (%) during de-and remineralization

Standard Deviation (SD) is in the parentheses.

jkacd-35-96-i005.jpg
Table 6
Remineralization amount (mm2) and value change (%) during de-and remineralization

Standard Deviation (SD) is in the parentheses.

jkacd-35-96-i006.jpg

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        The remineralization aspect of enamel according to change of the degree of saturation of the organic acid buffering solution in pH 5.5
        J Korean Acad Conserv Dent. 2010;35(2):96-105.   Published online March 31, 2010
        DOI: https://doi.org/10.5395/JKACD.2010.35.2.096
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      The remineralization aspect of enamel according to change of the degree of saturation of the organic acid buffering solution in pH 5.5
      Image Image Image Image Image Image Image Image Image Image Image Image Image Image Image
      Figure 1 Polarizing microscopic observation of demineralized enamel (Group 1, × 100).
      Figure 2 Polarizing microscopic observation of remineralized enamel (Group 1, × 100).
      Figure 3 Polarizing microscopic observation of demineralized enamel (Group 2, × 100).
      Figure 4 Polarizing microscopic observation of remineralized enamel (Group 2, × 100).
      Figure 5 Polarizing microscopic observation of demineralized enamel (Group 3, × 100).
      Figure 6 Polarizing microscopic observation of remineralized enamel (Group 3, × 100).
      Figure 7 Polarizing microscopic observation of demineralized enamel (Group 4, × 100).
      Figure 8 Polarizing microscopic observation of remineralized enamel (Group 4, × 100).
      Figure 9 Change ratio of demineralized depth (demineralized depth after remineralization / demineralized depth before remineralization) × 100 (%).
      Figure 10 Change rate of surface lesion width (surface lesion width after remineralization / surface lesion width before remineralization) × 100 (%).
      Figure 11 Comparison of density in enamel area before and after remineralization (Group 1).
      Figure 12 Comparison of density in enamel area before and after remineralization (Group 2).
      Figure 13 Comparison of density in enamel area before and after remineralization (Group 3).
      Figure 14 Comparison of density in enamel area before and after remineralization (Group 4).
      Figure 15 Change rate of remineralizalized amount before and after remineralization ((demineralized area before remineralization-demineralized area after remineralization/demineralized area before remineralization) ×100 + 100 (%)).

      Figure 1

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      Figure 11

      Figure 12

      Figure 13

      Figure 14

      Figure 15

      The remineralization aspect of enamel according to change of the degree of saturation of the organic acid buffering solution in pH 5.5

      Initial composition of demineralization solution

      Initial composition of remineralization solution

      One-way ANOVA on the remineralizing features of pH 5.5 solutions of different degree of saturations on artificially demineralized enamel

      * p < .05

      Demineralization depth (mm) and value change (%) during de- and remineralization

      Standard Deviation(SD) is in the parentheses.

      Surface lesion width (mm) and value change (%) during de-and remineralization

      Standard Deviation (SD) is in the parentheses.

      Remineralization amount (mm2) and value change (%) during de-and remineralization

      Standard Deviation (SD) is in the parentheses.
      Table 1 Initial composition of demineralization solution

      Table 2 Initial composition of remineralization solution

      Table 3 One-way ANOVA on the remineralizing features of pH 5.5 solutions of different degree of saturations on artificially demineralized enamel

      * p < .05

      Table 4 Demineralization depth (mm) and value change (%) during de- and remineralization

      Standard Deviation(SD) is in the parentheses.

      Table 5 Surface lesion width (mm) and value change (%) during de-and remineralization

      Standard Deviation (SD) is in the parentheses.

      Table 6 Remineralization amount (mm2) and value change (%) during de-and remineralization

      Standard Deviation (SD) is in the parentheses.

      Table 1

      Table 2

      Table 3

      Table 4

      Table 5

      Table 6

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