Restorative Dentistry & Endodontics

Research Articles

Cytotoxicity of newly developed pozzolan cement and other root-end filling materials on human periodontal ligament cell: Minju Song, Tae-Sun Yoon, Sue-Youn Kim, Euiseong Kim; Restor Dent Endod 2014;39(1):39-44. Published online January 20, 2014; DOI: https://doi.org/10.5395/rde.2014.39.1.39

Objectives

The purpose of this study was to evaluate in vitro cytotoxicity of the pozzolan cement and other root-end filling materials using human periodontal ligament cell.

Materials and Methods

Endocem (Maruchi), white ProRoot MTA (Dentsply), white Angelus MTA (Angelus), and Super EBA (Bosworth Co.) were tested after set completely in an incubator at 37℃ for 7 days, Endocem was tested in two ways: 1) immediately after mixing (fresh specimens) and 2) after setting completely like other experimental materials. The methods for assessment included light microscopic examination, cell counting and WST-1 assay on human periodontal ligament cell.

Results

In the results of microscopic examination and cell counting, Super EBA showed significantly lower viable cell than any other groups (p < 0.05). As the results of WST-1 assay, compared with untreated control group, there was no significant cell viability of the Endocem group. However, the fresh mixed Endocem group had significantly less cell viability. The cells exposed to ProRoot MTA and Angelus MTA showed the highest viability, whereas the cells exposed to Super EBA displayed the lowest viability (p < 0.05).

Conclusions

The cytotoxicity of the pozzolan cement (Endocem) was comparable with ProRoot MTA and Angelus MTA. Considering the difficult manipulation and long setting time of ProRoot MTA and Angelus MTA, Endocem can be used as the alternative of retrofilling material.

Citations

Citations to this article as recorded by

Effects of Three Retrograde Filling Materials on Production of Inflammatory Cytokines and Resorbing Mediators
Samaneh Arab, Marjan Bahraminasab, Masoumeh Motamedi, Jamshid Hadjati, Alaviye Vahid
Journal of Microbiota.2024;[Epub] CrossRef
Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material
Kazumasa Murata, Ayako Washio, Takahiko Morotomi, Thira Rojasawasthien, Shoichiro Kokabu, Chiaki Kitamura
Nanomaterials.2021; 11(7): 1828. CrossRef
Cell migration and osteo/odontogenesis stimulation of iRoot FS as a potential apical barrier material in apexification
Y. Liu, X. M. Liu, J. Bi, S. Yu, N. Yang, B. Song, X. Chen
International Endodontic Journal.2020; 53(4): 467. CrossRef
Biocompatibility of Biodentine™ ® with Periodontal Ligament Stem Cells: In Vitro Study
Duaa Abuarqoub, Nazneen Aslam, Hanan Jafar, Zakariya Abu Harfil, Abdalla Awidi
Dentistry Journal.2020; 8(1): 17. CrossRef
A micro-computed tomographic study of remaining filling materials of two bioceramic sealers and epoxy resin sealer after retreatment
KyungJae Kim, Da Vin Kim, Sin-Young Kim, SungEun Yang
Restorative Dentistry & Endodontics.2019;[Epub] CrossRef
Comparison of Gap Volume after Retrofilling Using 4 Different Filling Materials: Evaluation by Micro–computed Tomography
Sue Youn Kim, Hyeon-Cheol Kim, Su-Jung Shin, Euiseong Kim
Journal of Endodontics.2018; 44(4): 635. CrossRef
Anti-inflammatory and Mineralization Effects of ProRoot MTA and Endocem MTA in Studies of Human and Rat Dental Pulps In Vitro and In Vivo
Do-Hee Kim, Ji-Hyun Jang, Bin-Na Lee, Hoon-Sang Chang, In-Nam Hwang, Won-Mann Oh, Sun-Hun Kim, Kyung-San Min, Jeong-Tae Koh, Yun-Chan Hwang
Journal of Endodontics.2018; 44(10): 1534. CrossRef
Effects of Three Calcium Silicate Cements on Inflammatory Response and Mineralization-Inducing Potentials in a Dog Pulpotomy Model
Chung-Min Kang, Jiwon Hwang, Je Seon Song, Jae-Ho Lee, Hyung-Jun Choi, Yooseok Shin
Materials.2018; 11(6): 899. CrossRef
Cytocompatibility of Biodentine and iRoot FS with human periodontal ligament cells: an in vitro study
T. Luo, J. Liu, Y. Sun, Y. Shen, L. Zou
International Endodontic Journal.2018; 51(7): 779. CrossRef
Biological response of commercially available different tricalcium silicate-based cements and pozzolan cement
Serhat Köseoğlu, Tuğba Pekbağryank, Ebru Kucukyilmaz, Mehmet Sağlam, Sukru Enhos, Ayşe Akgün
Microscopy Research and Technique.2017; 80(9): 994. CrossRef
Biological efficacy of two mineral trioxide aggregate (MTA)-based materials in a canine model of pulpotomy
Myeongyeon LEE, Chung-Min KANG, Je Seon SONG, Yooseok SHIN, Seunghye KIM, Seong-Oh KIM, Hyung-Jun CHOI
Dental Materials Journal.2017; 36(1): 41. CrossRef
Cytotoxicities and genotoxicities of cements based on calcium silicate and of dental formocresol
Hyunjung Ko, Youngdan Jeong, Miri Kim
Mutation Research/Genetic Toxicology and Environmental Mutagenesis.2017; 815: 28. CrossRef
A Randomized Controlled Study of Mineral Trioxide Aggregate and Super Ethoxybenzoic Acid as Root-end Filling Materials in Endodontic Microsurgery: Long-term Outcomes
Sunil Kim, Minju Song, Su-Jung Shin, Euiseong Kim
Journal of Endodontics.2016; 42(7): 997. CrossRef
Effects of two fast-setting calcium-silicate cements on cell viability and angiogenic factor release in human pulp-derived cells
Chooryung J. Chung, Euiseong Kim, Minju Song, Jeong-Won Park, Su-Jung Shin
Odontology.2016; 104(2): 143. CrossRef
Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials
Diana María Escobar-García, Eva Aguirre-López, Verónica Méndez-González, Amaury Pozos-Guillén
BioMed Research International.2016; 2016: 1. CrossRef
In Vitro Cytotoxicity Evaluation of Three Root-End Filling Materials in Human Periodontal Ligament Fibroblasts
Hernán Coaguila-Llerena, Abraham Vaisberg, Zulema Velásquez-Huamán
Brazilian Dental Journal.2016; 27(2): 187. CrossRef
Dynamic intratubular biomineralization following root canal obturation with pozzolan‐based mineral trioxide aggregate sealer cement
Yeon‐Jee Yoo, Seung‐Ho Baek, Kee‐Yeon Kum, Won‐Jun Shon, Kyung‐Mi Woo, WooCheol Lee
Scanning.2016; 38(1): 50. CrossRef
A Randomized Controlled Study of the Use of ProRoot Mineral Trioxide Aggregate and Endocem as Direct Pulp Capping Materials
Minju Song, Minji Kang, Hyeon-Cheol Kim, Euiseong Kim
Journal of Endodontics.2015; 41(1): 11. CrossRef
Comparative Analysis of Selected Physicochemical Properties of Pozzolan Portland and MTA-Based Cements
Maura Cristiane Gonçales Orçati Dorileo, Ricardo Dalla Villa, Orlando Aguirre Guedes, Andreza Maria Fábio Aranha, Alex Semenoff-Segundo, Matheus Coelho Bandeca, Alvaro Henrique Borges
International Scholarly Research Notices.2014; 2014: 1. CrossRef
Surgical endodontics: past, present, and future
James L. Gutmann
Endodontic Topics.2014; 30(1): 29. CrossRef
Comparative analysis of physicochemical properties of root perforation sealer materials
Maura Cristiane Gonçales Orçati Dorileo, Fábio Luis Miranda Pedro, Matheus Coelho Bandeca, Orlando Aguirre Guedes, Ricardo Dalla Villa, Alvaro Henrique Borges
Restorative Dentistry & Endodontics.2014; 39(3): 201. CrossRef

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Washout resistance of fast-setting pozzolan cement under various root canal irrigants: Ga-Yeon Jang, Su-Jung Park, Seok-Mo Heo, Mi-Kyung Yu, Kwang-Won Lee, Kyung-San Min; Restor Dent Endod 2013;38(4):248-252. Published online November 12, 2013; DOI: https://doi.org/10.5395/rde.2013.38.4.248

Abstract PDF PubReader ePub

Objectives

Fast-setting pozzolan cement (Endocem, Maruchi) was recently developed. The aim of this study was to investigate the effects of various root canal irrigants on the washout of Endocem in comparison to the previously marketed mineral trioxide aggregate (ProRoot; Dentsply) in a furcal perforation model.

Materials and Methods

ProRoot and Endocem were placed into acrylic molds on moist Oasis. Each mold was then immediately exposed to either physiologic saline, 2.5% sodium hypochlorite (NaOCl), or 2% chlorhexidine (CHX) under gentle shaking for five minutes. Washout testing was performed by scoring scanning electron microscope (SEM) images.

Results

Endocem exhibited higher washout resistance compared to ProRoot, especially in the NaOCl group.

Conclusions

These results suggest that Endocem can be considered a useful repair material for furcal perforation, especially in a single-visit scenario.

Citations

Citations to this article as recorded by

The Washout Resistance of Bioactive Root-End Filling Materials—A Systematic Review
Joanna Falkowska-Ostrowska, Włodzimierz Dura
Journal of Clinical Medicine.2025; 14(7): 2446. CrossRef
Stereomicroscopic Evaluation of Sealing Ability of Three Different Furcal Perforation Repair Materials: An In vitro Study
Sriparna De, N Sathyajith Naik, Shivangi Sharma, Pallavi Vashisth, Rasleen Dua, Priya Maheshwari
Contemporary Clinical Dentistry.2024; 15(4): 259. CrossRef
Chemical and physical properties of radiopaque Portland cement formulation with reduced particle size
Hoda Mohamed ELNAWAWY, Muralithran Govindan KUTTY, Noor Azlin YAHYA, Noor Hayaty ABU KASIM, Paul Roy COOPER, Josette CAMILLERI, Hany Mohamed Aly AHMED
Dental Materials Journal.2024; 43(5): 672. CrossRef
The Washout Resistance of Bioactive Root-End Filling Materials
Joanna Falkowska, Tomasz Chady, Włodzimierz Dura, Agnieszka Droździk, Małgorzata Tomasik, Ewa Marek, Krzysztof Safranow, Mariusz Lipski
Materials.2023; 16(17): 5757. CrossRef
Effects of fast- and slow-setting calcium silicate–based root-end filling materials on the outcome of endodontic microsurgery: a retrospective study up to 6 years
Dohyun Kim, Hyunjung Lee, Minsun Chung, Sunil Kim, Minju Song, Euiseong Kim
Clinical Oral Investigations.2020; 24(1): 247. CrossRef
Novel anti-biofouling bioactive calcium silicate-based cement containing 2-methacryloyloxyethyl phosphorylcholine
Jae-Sung Kwon, Myung-Jin Lee, Ji-Young Kim, Dohyun Kim, Jeong-Hyun Ryu, Sungil Jang, Kwang-Mahn Kim, Chung-Ju Hwang, Sung-Hwan Choi, Jinkee Hong
PLOS ONE.2019; 14(1): e0211007. CrossRef
Surface and vertical dimensional changes of mineral trioxide aggregate and biodentine in different environmental conditions
Hacer Aksel, Selen Küçükkaya Eren, Sevinc Askerbeyli Õrs, Eda Karaismailoğlu
Journal of Applied Oral Science.2018;[Epub] CrossRef
Push-out Bond Strength of Fast-setting Mineral Trioxide Aggregate and Pozzolan-based Cements: ENDOCEM MTA and ENDOCEM Zr
Emmanuel João Nogueira Leal Silva, Nancy Kudsi Carvalho, Marta Reis da Costa Labanca Guberman, Marina Prado, Plinio Mendes Senna, Erick M. Souza, Gustavo De-Deus
Journal of Endodontics.2017; 43(5): 801. CrossRef
Dynamic intratubular biomineralization following root canal obturation with pozzolan‐based mineral trioxide aggregate sealer cement
Yeon‐Jee Yoo, Seung‐Ho Baek, Kee‐Yeon Kum, Won‐Jun Shon, Kyung‐Mi Woo, WooCheol Lee
Scanning.2016; 38(1): 50. CrossRef
Comparison of Setting Time, Compressive Strength, Solubility, and pH of Four Kinds of MTA
Jing-Ling Che, Jae-Hwan Kim, Seon-Mi Kim, Nam-ki Choi, Hyun-Joo Moon, Moon-Jin Hwang, Ho-Jun Song, Yeong-Joon Park
Korean Journal of Dental Materials.2016; 43(1): 61. CrossRef
A Randomized Controlled Study of the Use of ProRoot Mineral Trioxide Aggregate and Endocem as Direct Pulp Capping Materials: 3-month versus 1-year Outcomes
Youngjune Jang, Minju Song, Il-Sang Yoo, Yunjung Song, Byoung-Duck Roh, Euiseong Kim
Journal of Endodontics.2015; 41(8): 1201. CrossRef
Odontogenic effects of a fast-setting calcium-silicate cement containing zirconium oxide
Kyoung-A KIM, Yeon-Mi YANG, Young-Sun KWON, Yun-Chan HWANG, Mi-Kyung YU, Kyung-San MIN
Dental Materials Journal.2015; 34(4): 432. CrossRef
D90: The Strongest Contributor to Setting Time in Mineral Trioxide Aggregate and Portland Cement
William N. Ha, Dale P. Bentz, Bill Kahler, Laurence J. Walsh
Journal of Endodontics.2015; 41(7): 1146. CrossRef
A Randomized Controlled Study of the Use of ProRoot Mineral Trioxide Aggregate and Endocem as Direct Pulp Capping Materials
Minju Song, Minji Kang, Hyeon-Cheol Kim, Euiseong Kim
Journal of Endodontics.2015; 41(1): 11. CrossRef
Physical properties and biological/odontogenic effects of an experimentally developed fast-setting α-tricalcium phosphate-based pulp capping material
Jun-Bong Lee, Su-Jung Park, Hyun-Ha Kim, Young-Sun Kwon, Kwang-Won Lee, Kyung-San Min
BMC Oral Health.2014;[Epub] CrossRef
Effect of Mineral Trioxide Aggregate Surface Treatments on Morphology and Bond Strength to Composite Resin
Joo-Hee Shin, Ji-Hyun Jang, Sang Hyuk Park, Euiseong Kim
Journal of Endodontics.2014; 40(8): 1210. CrossRef

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