KACD
Search
- Page Path
- HOME > Search
Research Article
- Effect of medium or high concentrations of in-office dental bleaching gel on the human pulp response in the mandibular incisors
- Douglas Augusto Roderjan, Rodrigo Stanislawczuk, Diana Gabriela Soares, Carlos Alberto de Souza Costa, Michael Willian Favoreto, Alessandra Reis, Alessandro D. Loguercio
- Restor Dent Endod 2023;48(2):e12. Published online March 8, 2023
- DOI: https://doi.org/10.5395/rde.2023.48.e12
-
Abstract
PDF
PubReader
ePub Objectives The present study evaluated the pulp response of human mandibular incisors subjected to in-office dental bleaching using gels with medium or high concentrations of hydrogen peroxide (HP).
Materials and Methods The following groups were compared: 35% HP (HP35;
n = 5) or 20% HP (HP20;n = 4). In the control group (CONT;n = 2), no dental bleaching was performed. The color change (CC) was registered at baseline and after 2 days using the Vita Classical shade guide. Tooth sensitivity (TS) was also recorded for 2 days post-bleaching. The teeth were extracted 2 days after the clinical procedure and subjected to histological analysis. The CC and overall scores for histological evaluation were evaluated by the Kruskal-Wallis and Mann-Whitney tests. The percentage of patients with TS was evaluated by the Fisher exact test (α = 0.05).Results The CC and TS of the HP35 group were significantly higher than those of the CONT group (
p < 0.05) and the HP20 group showed an intermediate response, without significant differences from either the HP35 or CONT group (p > 0.05). In both experimental groups, the coronal pulp tissue exhibited partial necrosis associated with tertiary dentin deposition. Overall, the subjacent pulp tissue exhibited a mild inflammatory response.Conclusions In-office bleaching therapies using bleaching gels with 20% or 35% HP caused similar pulp damage to the mandibular incisors, characterized by partial necrosis, tertiary dentin deposition, and mild inflammation.
- 29 View
- 1 Download
- 6 Web of Science
Original Article
- Expression and functional characterization of odontoblast-derived gene: OD314
- Doo-Hyun Kim, Heung-Joong Kim, Moon-Jin Jeong, Ho-Hyun Son, Joo-Cheol Park
- J Korean Acad Conserv Dent 2004;29(4):399-408. Published online July 31, 2004
- DOI: https://doi.org/10.5395/JKACD.2004.29.4.399
-
Abstract
PDFPubReaderePub
Odontoblasts are responsible for the formation and maintenance of dentin. They are known to synthesize unique gene products including dentin sialophosphoprotein (DSPP). Another unique genes of the cells remain unclear.
OD314 was isolated from the odontoblasts/pulp cells of rats and partially characterized as an odontoblast-enriched gene (Dey et al., 2001). This study aimed to elucidate the biological function of OD314, relating to odontoblast differentiation and dentinogenesis. After determining the open reading frame (ORF) of OD314 by transient transfection analysis using green fluorescent protein (GFP) expression vector, mRNA
in-situ hybridization, immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR) and western analysis were performed.The results were as follows:
1. In
in-situ hybridization, OD314 mRNAs were expressed in odontoblasts of developing coronal and root pulp.2. OD314 was a novel protein encoding 154 amino acids, and the protein was mainly expressed in cytoplasm by transient transfection analysis.
3. Mineralized nodules were associated with multilayer cell nodules in the culture of human dental pulp cells and first detected from day 21 using alizarin-red S staining.
4. In RT-PCR analysis, OD314, osteocalcin (OC) and DSPP strongly expressed throughout 28 days of culture. Whereas, osteonectin (ON) mRNA expression stayed low up to day 14, and then gradually decreased from day 21.
5. Western blots showed an approximately 17 kDa band. OD314 protein was expressed from the start of culture and then increased greatly from day 21.
In conclusion, OD314 is considered as an odontoblast-enriched gene and may play important roles in odontoblast differentiation and dentin mineralization.
- 23 View
- 0 Download
First
Prev
