The purposes of this study were to examine the variability of adhesive thickness on the different site of the cavity wall when used total-etch system without filler and simplified self-etch system with filler and to evaluate the relationship between variable adhesive thickness and microtensile bond strength to the cavity wall.

A class I cavity in six human molars was prepared to expose all dentinal walls. Three teeth were bonded with a filled adhesive, Clearfil™ SE bond and the other three teeth were bonded with unfilled adhesives, Scotchbond™ Multi Purpose. Morphology and thickness of adhesive layer were examined using fluorescence microscope. Bonding agent thickness was measured at three points along the axial cavity wall, edge of cavity margin (rim), halfway down each cavity wall (hlf), internal angle of the cavity (ang). After reproducing the adhesive thickness at rim, hlf and ang, micro-tensile bond strength were evaluated.

For both bonding agents, adhesive thickness of ang was significantly thicker than that of rim and hlf (P < 0.05). As reproduced the adhesive thickness, microtensile bond strength was increased as adhesive thickness was increased in two bonding agents.

Adhesive thickness of internal angle of the cavity was significantly thicker than that of the cavity margin and the halfway cavity wall for both bonding agents. Microtensile bond strength of the thick adhesive layer at the internal angle of the cavity was higher than that of the thin adhesive layer at the cavity margin and the halfway cavity in the two bonding systems.