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Research Articles
The effect of saliva decontamination procedures on dentin bond strength after universal adhesive curing
Jayang Kim, Sungok Hong, Yoorina Choi, Sujung Park
Restor Dent Endod 2015;40(4):299-305.   Published online October 2, 2015
DOI: https://doi.org/10.5395/rde.2015.40.4.299
AbstractAbstract PDFPubReaderePub
Objectives

The purpose of this study was to investigate the effectiveness of multiple decontamination procedures for salivary contamination after curing of a universal adhesive on dentin bond strength according to its etch modes.

Materials and Methods

Forty-two extracted bovine incisors were trimmed by exposing the labial dentin surfaces and embedded in cylindrical molds. A universal adhesive (All-Bond Universal, Bisco) was used. The teeth were randomly divided into groups according to etch mode and decontamination procedure. The adhesive was applied according to the manufacturer's instructions for a given etch mode. With the exception of the control groups, the cured adhesive was contaminated with saliva for 20 sec. In the self-etch group, the teeth were divided into three groups: control, decontamination with rinsing and drying, and decontamination with rinsing, drying, and adhesive. In the etch-and-rinse group, the teeth were divided into four groups: control, decontamination with rinsing and drying, decontamination with rinsing, drying, and adhesive, and decontamination with rinsing, drying, re-etching, and reapplication of adhesive. A composite resin (Filtek Z350XT, 3M ESPE) was used for filling and was cured on the treated surfaces. Shear bond strength was measured, and failure modes were evaluated. The data were subjected to one-way analysis of variation and Tukey's HSD test.

Results

The etch-and-rinse subgroup that was decontaminated by rinse, drying, re-etching, and reapplication of adhesive showed a significantly higher bond strength.

Conclusions

When salivary contamination occurs after curing of the universal adhesive, additional etching improves the bond strength to dentin.

Citations

Citations to this article as recorded by  
  • Effect of contamination and decontamination methods on the bond strength of adhesive systems to dentin: A systematic review
    Rim Bourgi, Carlos Enrique Cuevas‐Suarez, Walter Devoto, Ana Josefina Monjarás‐Ávila, Paulo Monteiro, Khalil Kharma, Monika Lukomska‐Szymanska, Louis Hardan
    Journal of Esthetic and Restorative Dentistry.2023; 35(8): 1218.     CrossRef
  • Universal adhesive application to contaminated/non-contaminated dentin with three different protocols: An in vitro shear bond strength and SEM analysis
    Tuğçe BALOGLU GONCU, Nasibe Aycan YILMAZ
    Dental Materials Journal.2022; 41(4): 633.     CrossRef
  • Tükürük kontaminasyon/dekontaminasyonunun üniversal adezivlerin dentine bağlanma dayanımına etkisi
    Cansu ATALAY, Aybüke USLU, Ece MERAL, Ayşe YAZICI, A. Atila ERTAN
    Selcuk Dental Journal.2021; 8(3): 611.     CrossRef
  • Bioactive glass ceramic can improve the bond strength of sealant/enamel?
    R. E. Silveira, R. G. Vivanco, R. C. de Morais, G. Da Col dos Santos Pinto, F. de C. P. Pires-de-Souza
    European Archives of Paediatric Dentistry.2019; 20(4): 325.     CrossRef
  • Universal dental adhesives: Current status, laboratory testing, and clinical performance
    Sanket Nagarkar, Nicole Theis‐Mahon, Jorge Perdigão
    Journal of Biomedical Materials Research Part B: Applied Biomaterials.2019; 107(6): 2121.     CrossRef
  • Effect of Saliva Decontamination on Bond Strength of 1-step Self-etching Adhesives to Dentin of Primary Posterior Teeth
    Junhee Lee, Shin Kim, Taesung Jeong, Jonghyun Shin, Eungyung Lee, Jiyeon Kim
    THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY.2019; 46(3): 274.     CrossRef
  • Polymeric materials and films in dentistry: An overview
    Dinesh Rokaya, Viritpon Srimaneepong, Janak Sapkota, Jiaqian Qin, Krisana Siraleartmukul, Vilailuck Siriwongrungson
    Journal of Advanced Research.2018; 14: 25.     CrossRef
  • Cytotoxicity of Light-Cured Dental Materials according to Different Sample Preparation Methods
    Myung-Jin Lee, Mi-Joo Kim, Jae-Sung Kwon, Sang-Bae Lee, Kwang-Mahn Kim
    Materials.2017; 10(3): 288.     CrossRef
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Evaluation of the effect of blood contamination on the compressive strength of MTA modified with hydration accelerators
Kaveh Oloomi, Eshaghali Saberi, Hadi Mokhtari, Hamid Reza Mokhtari Zonouzi, Ali Nosrat, Mohammad Hossein Nekoofar, Paul Michael Howell Dummer
Restor Dent Endod 2013;38(3):128-133.   Published online August 23, 2013
DOI: https://doi.org/10.5395/rde.2013.38.3.128
AbstractAbstract PDFPubReaderePub
Objectives

This study was performed to evaluate the effect of blood contamination on the compressive strength (CS) of Root MTA (RMTA) modified with Calcium chloride (CaCl2) and Disodium hydrogen phosphate (Na2HPO4) as setting accelerators over time.

Materials and Methods

A total of 110 cylindrical specimens of RMTA were divided into 6 experimental groups as follows: Group1, RMTA; Group 2, RMTA modified with CaCl2 (RMTA-C); Group 3, RMTA modified with Na2HPO4 (RMTA-N); Group 4, RMTA contaminated with blood; Group 5, RMTA-C contaminated with blood; Group 6, RMTA-N contaminated with blood. The CS of specimens in all groups was evaluated after 3 hr, 24 hr, and 1 wk. In the modified groups (groups 2, 3, 5, and 6) the CS of five specimens per group was also evaluated after 1 hr.

Results

Blood contamination significantly reduced the CS of all materials at all time intervals (p < 0.05). After 3 hr, the CS of specimens in the RMTA groups (with and without blood contamination) was significantly lower than those in the RMTA-C and RMTA-N groups (p < 0.05). The CS values were not significantly different at the other time intervals. In all groups, the CS of specimens significantly increased over time (p < 0.05).

Conclusions

Blood contamination decreased the CS of both original and accelerated RMTA.

Citations

Citations to this article as recorded by  
  • The effect of three additives on properties of mineral trioxide aggregate cements: a systematic review and meta-analysis of in vitro studies
    Behnam Bolhari, Faranak Noori, Hadi Assadian, Amir Raee, Sholeh Ghabraei, Ahmad-Reza Shamshiri, Artak Heboyan
    BMC Oral Health.2024;[Epub]     CrossRef
  • Push‐out bond strength of the calcium silicate‐based endodontic cements in the presence of blood: A systematic review and meta‐analysis of in vitro studies
    Mahdieh Alipour, Leili Faraji Gavgani, Negin Ghasemi
    Clinical and Experimental Dental Research.2022; 8(2): 571.     CrossRef
  • Effect of bioactive glass addition on the physical properties of mineral trioxide aggregate
    Jei Kim, Hyun-Jung Kim, Seok Woo Chang, Soram Oh, Sun-Young Kim, Kyoung-Kyu Choi, Duck-Su Kim, Ji-Hyun Jang
    Biomaterials Research.2021;[Epub]     CrossRef
  • Effect of the addition of nanoparticles of CaCO3 and different water‐to‐powder ratios on the physicochemical properties of white Portland cement
    Cleonice da Silveira Teixeira, Jessica Coelho Wasielewsky, Giovanna Slongo dos Santos, Anarela Bernardi, Eduardo Antunes Bortoluzzi, Lucas da Fonseca Roberti Garcia
    Microscopy Research and Technique.2021; 84(4): 592.     CrossRef
  • Assessment of the interaction of Portland cement-based materials with blood and tissue fluids using an animal model
    P. Schembri Wismayer, C. Y. K. Lung, F. Rappa, F. Cappello, J. Camilleri
    Scientific Reports.2016;[Epub]     CrossRef
  • Effect of the plant-based hemostatic agent Ankaferd Blood Stopper® on the biocompatibility of mineral trioxide aggregate
    Muzaffer Emir Dinçol, Hakan Ozbas, Bulent Yılmaz, Handan Ersev, Selcuk Gokyay, Vakur Olgac
    BMC Oral Health.2016;[Epub]     CrossRef
  • Surface microhardness of three thicknesses of mineral trioxide aggregate in different setting conditions
    Noushin Shokouhinejad, Leila Jafargholizadeh, Mehrfam Khoshkhounejad, Mohammad Hossein Nekoofar, Maryam Raoof
    Restorative Dentistry & Endodontics.2014; 39(4): 253.     CrossRef
  • Surgical management of a failed internal root resorption treatment: a histological and clinical report
    Saeed Asgary, Mohammad Jafar Eghbal, Leili Mehrdad, Sanam Kheirieh, Ali Nosrat
    Restorative Dentistry & Endodontics.2014; 39(2): 137.     CrossRef
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  • 8 Crossref
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Original Article
Effect of salivary contamination of teeth on microtensile bond strength of various dentin bonding systems
Kyoung-Kyu Choi, Gil-Joo Ryu
J Korean Acad Conserv Dent 2003;28(3):203-208.   Published online May 31, 2003
DOI: https://doi.org/10.5395/JKACD.2003.28.3.203
AbstractAbstract PDFPubReaderePub

The purpose of this study was to evaluate the effect of salivary contamination of teeth on bonding efficacy of self-priming and self-etching DBSs. The materials used were Single Bond(SB, self-priming system, 3M), Unifil Bond(UB, self-etching system, GC), and Scotchbond Multi-Purpose Plus(SM, 3M) as control. Forty five human molars randomly allocated to three groups as dentin bonding systems tested and embedded in epoxy resin. Then the specimens were wet-ground to expose flat buccal enamel surface or flat occlusal dentin surface and cut bucco-lingually to form two halves with slow speed diamond saw. One of them was used under non-contamination, other under contamination with saliva. The bonding procedure was according to the manufacturer's directions and resin composite(Z-100, 3M Dental Products, St. Paul, MN) was built-up on the bonded surface 5mm high. The specimens were ground carefully at the enamel-composite interface with fine finishing round diamond bur to create an hour-glass shape yielding bonded surface areas of 1.5±0.1mm2. The specimens were bonded to the modified microtensile testing apparatus with cyanoacrylate, attached to the universal testing machine and stressed in tension at a CHS of 1mm/min.. The tensile force at failure was recorded and converted to a tensile stress(MPa). Mean values and standard deviations of the bond strength are listed in table. One-way ANOVA was used to determine significant difference at the 95% level.

The bond strength of SBMP and SB were not affected by salivary contamination, but that of UB was significantly affected by salivary contamination. These results indicate that DBSs with total etch technique seems less likely affected by salivary contamination in bonding procedure.

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