Restorative Dentistry & Endodontics

Young-Eun Jang

2 Articles

Changes in SIRT gene expression during odontoblastic differentiation of human dental pulp cells: Young-Eun Jang, Su-Hee Go, Bin-Na Lee, Hoon-Sang Chang, In-Nam Hwang, Won-Mann Oh, Yun-Chan Hwang; Restor Dent Endod 2015;40(3):223-228. Published online July 15, 2015; DOI: https://doi.org/10.5395/rde.2015.40.3.223

Objectives

The aim of this study was to investigate the expression of 7 different sirtuin genes (SIRT1-SIRT7) in human dental pulp cells (HDPCs), and to determine the role of SIRTs in the odontoblastic differentiation potential of HDPCs.

Materials and Methods

HDPCs were isolated from freshly extracted third molar teeth of healthy patients and cultulred in odontoblastic differentiation inducing media. Osteocalcin (OCN) and dentin sialophosphoprotein (DSPP) expression was analyzed to evaluate the odontoblastic differentiation of HDPCs by reverse transcription-polymerase chain reaction (RT-PCR), while alizarin red staining was used for the mineralization assay. To investigate the expression of SIRTs during odontoblastic differentiation of HDPCs, real time PCR was also performed with RT-PCR.

Results

During the culture of HDPCs in the differentiation inducing media, OCN, and DSPP mRNA expressions were increased. Mineralized nodule formation was also increased in the 14 days culture. All seven SIRT genes were expressed during the odontogenic induction period. SIRT4 expression was increased in a time-dependent manner.

Conclusions

Our study identified the expression of seven different SIRT genes in HDPCs, and revealed that SIRT4 could exert an influence on the odontoblast differentiation process. Further studies are needed to determine the effects of other SIRTs on the odontogenic potential of HDPCs.

Citations

Citations to this article as recorded by

Biodegradable Zn‐5Dy Alloy with Enhanced Osteo/Angio‐Genic Activity and Osteointegration Effect via Regulation of SIRT4‐Dependent Mitochondrial Function
Yue Han, Xian Tong, Runqi Zhou, Yilin Wang, Yuge Chen, Liang Chen, Xinhua Hong, Linmei Wu, Zhiqiang Lin, Yichi Zhang, Xuejia Zhang, Chaoming Hu, Bin Li, Yifan Ping, Zelin Cao, Zhou Ye, Zhongchen Song, Yuncang Li, Cuie Wen, Yongsheng Zhou, Jixing Lin, Shen
Advanced Science.2024;[Epub] CrossRef
The Role of Histone Acetylation Modification in Dental Tissue-Derived Mesenchymal Stem Cells and Odontogenesis
Haoling Chen, Zijing Huang, Chuxiao Chen
Cellular Reprogramming.2023; 25(1): 11. CrossRef
Metabolic Remodeling Impacts the Epigenetic Landscape of Dental Mesenchymal Stem Cells
Haiyun Luo, Yachuan Zhou, Wenjing Liu, Jun Wang
Stem Cells International.2022; 2022: 1. CrossRef
SIRT4 regulates rat dental papilla cell differentiation by promoting mitochondrial functions
Haoling Chen, Jun Kang, Fuping Zhang, Tong Yan, Wenguo Fan, Hongwen He, Fang Huang
The International Journal of Biochemistry & Cell Biology.2021; 134: 105962. CrossRef
Sirtuins as Interesting Players in the Course of HIV Infection and Comorbidities
Karolina Jurkowska, Beata Szymańska, Brygida Knysz, Amadeusz Kuźniarski, Agnieszka Piwowar
Cells.2021; 10(10): 2739. CrossRef
Robust expression of SIRT6 inhibits pulpitis via activation of the TRPV1 channel
Jia Hu, Weiran Chen, Zailing Qiu, Hongbing Lv
Cell Biochemistry and Function.2020; 38(5): 676. CrossRef
Downregulation of microRNA‐143‐5p is required for the promotion of odontoblasts differentiation of human dental pulp stem cells through the activation of the mitogen‐activated protein kinases 14‐dependent p38 mitogen‐activated protein kinases signaling pa
Bao‐Liang Wang, Zhi Wang, Xi Nan, Qing‐Cai Zhang, Wei Liu
Journal of Cellular Physiology.2019; 234(4): 4840. CrossRef
A potential role for the silent information regulator 2 homologue 1 (SIRT1) in periapical periodontitis
H. Kudo, O. Takeichi, K. Hatori, K. Makino, K. Himi, B. Ogiso
International Endodontic Journal.2018; 51(7): 747. CrossRef
Overexpressed Sirt1 in MSCs Promotes Dentin Formation in Bmi1-Deficient Mice
H. Wang, C. Lv, Y. Gu, Q. Li, L. Xie, H. Zhang, D. Miao, W. Sun
Journal of Dental Research.2018; 97(12): 1365. CrossRef
Expression of silent information regulator 2 homolog 1 (SIRT1) in periapical granulomas
Hiroshi Kudo, Osamu Takeichi, Kosuke Makino, Keisuke Hatori, Bunnai Ogiso
Journal of Oral Science.2018; 60(3): 411. CrossRef
TET1 knockdown inhibits the odontogenic differentiation potential of human dental pulp cells
Li-Jia Rao, Bai-Cheng Yi, Qi-Meng Li, Qiong Xu
International Journal of Oral Science.2016; 8(2): 110. CrossRef

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Cytotoxicity and physical properties of tricalcium silicate-based endodontic materials: Young-Eun Jang, Bin-Na Lee, Jeong-Tae Koh, Yeong-Joon Park, Nam-Eok Joo, Hoon-Sang Chang, In-Nam Hwang, Won-Mann Oh, Yun-Chan Hwang; Restor Dent Endod 2014;39(2):89-94. Published online March 21, 2014; DOI: https://doi.org/10.5395/rde.2014.39.2.89

Abstract PDF PubReader ePub

Objectives

The aim of this study was to evaluate the cytotoxicity, setting time and compressive strength of MTA and two novel tricalcium silicate-based endodontic materials, Bioaggregate (BA) and Biodentine (BD).

Materials and Methods

Cytotoxicity was evaluated by using a 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide (XTT) assay. Measurements of 9 heavy metals (arsenic, cadmium, chromium, copper, iron, lead, manganese, nickel, and zinc) were performed by inductively coupled plasma-mass spectrometry (ICP-MS) of leachates obtained by soaking the materials in distilled water. Setting time and compressive strength tests were performed following ISO requirements.

Results

BA had comparable cell viability to MTA, whereas the cell viability of BD was significantly lower than that of MTA. The ICP-MS analysis revealed that BD released significantly higher amount of 5 heavy metals (arsenic, copper, iron, manganese, and zinc) than MTA and BA. The setting time of BD was significantly shorter than that of MTA and BA, and the compressive strength of BA was significantly lower than that of MTA and BD.

Conclusions

BA and BD were biocompatible, and they did not show any cytotoxic effects on human periodontal ligament fibroblasts. BA showed comparable cytotoxicity to MTA but inferior physical properties. BD had somewhat higher cytotoxicity but superior physical properties than MTA.

Citations

Citations to this article as recorded by

Evaluation of the physical properties of bromelain-modified biodentine for direct pulp capping
Paridhi Agrawal, Manoj Chandak, Aditya Patel, Jay Bhopatkar
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Evaluation of bioactivity, biocompatibility, and antibacterial properties of tricalcium silicate bone cement modified with wollastonite/ fluorapatite glass and glass-ceramic
H.K. Abd El-Hamid, A.M. Fayad, R.L. Elwan
Ceramics International.2024; 50(14): 25322. CrossRef
Evaluation of the chemical, physical, and biological properties of a newly developed bioceramic cement derived from cockle shells: an in vitro study
Monthip Wannakajeepiboon, Chankhrit Sathorn, Chatvadee Kornsuthisopon, Busayarat Santiwong, Thanakorn Wasanapiarnpong, Pairoj Linsuwanont
BMC Oral Health.2023;[Epub] CrossRef
Strength of a cement-based dental material: Early age testing and first micromechanical modeling at mature age
Petr Dohnalík, Christian Hellmich, Gilles Richard, Bernhard L. A. Pichler
Frontiers in Bioengineering and Biotechnology.2023;[Epub] CrossRef
Calcium silicate and calcium aluminate cements for dentistry reviewed
Carolyn Primus, James L. Gutmann, Franklin R. Tay, Anna B. Fuks
Journal of the American Ceramic Society.2022; 105(3): 1841. CrossRef
Biomimetic Approaches in Clinical Endodontics
Naresh Kumar, Nazrah Maher, Faiza Amin, Hani Ghabbani, Muhammad Sohail Zafar, Francisco Javier Rodríguez-Lozano, Ricardo E. Oñate-Sánchez
Biomimetics.2022; 7(4): 229. CrossRef
Effect of different manipulations on the physical, chemical and microstructural characteristics of Biodentine
Mariana Domingos Pires, Joana Cordeiro, Isabel Vasconcelos, Mariana Alves, Sérgio André Quaresma, António Ginjeira, Josette Camilleri
Dental Materials.2021; 37(7): e399. CrossRef
Minimal Intervention in Dentistry: A Literature Review on Biodentine as a Bioactive Pulp Capping Material
Naji Ziad Arandi, Mohammad Thabet, Mona Abbassy
BioMed Research International.2021;[Epub] CrossRef
Chitosan-Based Accelerated Portland Cement Promotes Dentinogenic/Osteogenic Differentiation and Mineralization Activity of SHED
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Polymers.2021; 13(19): 3358. CrossRef
Material Pulp Cells and Tissue Interactions
Nastaran Meschi, Biraj Patel, Nikita B. Ruparel
Journal of Endodontics.2020; 46(9): S150. CrossRef
Biological Effects of Tricalcium Silicate Nanoparticle-Containing Cement on Stem Cells from Human Exfoliated Deciduous Teeth
Yoonsun Jung, Ji-Young Yoon, Kapil Dev Patel, Lan Ma, Hae-Hyoung Lee, Jongbin Kim, Jung-Hwan Lee, Jisun Shin
Nanomaterials.2020; 10(7): 1373. CrossRef
Physicochemical, mechanical and cytotoxicity evaluation of chitosan-based accelerated portland cement
Hasan Subhi, Adam Husein, Dasmawati Mohamad, Asma-Abdullah Nurul
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Tricalcium silicate cements: osteogenic and angiogenic responses of human bone marrow stem cells
Mohamed R. W. Ali, Manal Mustafa, Asgeir Bårdsen, Athanasia Bletsa
European Journal of Oral Sciences.2019; 127(3): 261. CrossRef
Bioactive tri/dicalcium silicate cements for treatment of pulpal and periapical tissues
Carolyn M. Primus, Franklin R. Tay, Li-na Niu
Acta Biomaterialia.2019; 96: 35. CrossRef
Effect of phytic acid on the setting times and tensile strengths of calcium silicate‐based cements
Ozgur Uyanik, Emre Nagas, Selen Kucukkaya Eren, Zafer C. Cehreli, Pekka K. Vallittu, Lippo V.J. Lassila
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Effects of four novel root-end filling materials on the viability of periodontal ligament fibroblasts
Makbule Bilge Akbulut, Pembegul Uyar Arpaci, Ayce Unverdi Eldeniz
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Biodentine™ material characteristics and clinical applications: a 3 year literature review and update
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Root perforations: a review of diagnosis, prognosis and materials
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Retention of BioAggregate and MTA as coronal plugs after intracanal medication for regenerative endodontic procedures: an ex vivo study
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Biological response of commercially available different tricalcium silicate-based cements and pozzolan cement
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Cytotoxic effects of mineral trioxide aggregate, calcium enrichedmixture cement, Biodentine and octacalcium pohosphate onhuman gingival fibroblasts
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The effect of working time on the displacement of Biodentine™ beneath prefabricated stainless steel crown: a laboratory study
Alaa E. Dawood, David J. Manton, Peter Parashos, Rebecca H. K. Wong
Journal of Investigative and Clinical Dentistry.2016; 7(4): 391. CrossRef
Evaluation of reparative dentin formation of ProRoot MTA, Biodentine and BioAggregate using micro-CT and immunohistochemistry
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Restorative Dentistry & Endodontics.2016; 41(1): 29. CrossRef

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