Restorative Dentistry & Endodontics

Yong-Beom Kim

3 Articles

Comparison of gene expression profiles of human dental pulp cells treated with mineral trioxide aggregate and calcium hydroxide: Yong-Beom Kim, Won-Jun Shon, Woocheol Lee, Kee-Yeon Kum, Seung-Ho Baek, Kwang-Shik Bae; J Korean Acad Conserv Dent 2011;36(5):397-408. Published online September 14, 2011; DOI: https://doi.org/10.5395/JKACD.2011.36.5.397

Abstract Objectives:

This study investigated changes in gene expressions concerning of differentiation, proliferation, mineralization and inflammation using Human-8 expression bead arrays when white Mineral Trioxide Aggregate and calcium hydroxide-containing cement were applied in vitro to human dental pulp cells (HDPCs).

Materials and Methods:

wMTA (white ProRoot MTA, Dentsply) and Dycal (Dentsply Caulk) in a Teflon tube (inner diameter 10 mm, height 1 mm) were applied to HDPCs. Empty tube-applied HDPCs were used as negative control. Total RNA was extracted at 3, 6, 9 and 24 hr after wMTA and Dycal application. The results of microarray were confirmed by reverse transcriptase polymerase chain reaction.

Results:

Out of the 24,546 genes, 43 genes (e.g., BMP2, FOSB, THBS1, EDN1, IL11, COL10A1, TUFT1, HMOX1) were up-regulated greater than two-fold and 25 genes (e.g., SMAD6, TIMP2, DCN, SOCS2, CEBPD, KIAA1199) were down-regulated below 50% by wMTA. Two hundred thirty nine genes (e.g., BMP2, BMP6, SMAD6, IL11, FOS, VEGFA, PlGF, HMOX1, SOCS2, CEBPD, KIAA1199) were up-regulated greater than two-fold and 358 genes (e.g., EDN1, FGF) were down-regulated below 50% by Dycal.

Conclusions:

Both wMTA and Dycal induced changes in gene expressions related with differentiation and proliferation of pulp cells. wMTA induced changes in gene expressions related with mineralization, and Dycal induced those related with angiogenesis. The genes related with inflammation were more expressed by Dycal than by wMTA. It was confirmed that both wMTA and Dycal were able to induce gene expression changes concerned with the pulp repair in different ways.

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Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells
Min-Seock Seo, Kyung-Gyun Hwang, Hyongbum Kim, Seung-Ho Baek
Restorative Dentistry & Endodontics.2012; 37(3): 142. CrossRef

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Gene expression profiling in human dental pulp cells treated with mineral trioxide aggregate: Yong-Beom Kim, Won-Jun Shon, WooCheol Lee, Kee-Yeon Kum, Seung-Ho Baek, Kwang-Shik Bae; J Korean Acad Conserv Dent 2010;35(3):152-163. Published online May 31, 2010; DOI: https://doi.org/10.5395/JKACD.2010.35.3.152

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This study investigated the changes in gene expression when mineral trioxide aggregate (MTA) was applied in vitro to human dental pulp cells (HDPCs). MTA in a teflon tube (diameter 10 mm, height 2 mm) was applied to HDPCs. Empty tube-applied HDPCs were used as negative control. For microarray analysis, total RNA was extracted at 6, 24, and 72 hrs after MTA application. The results were confirmed selectively by performing reverse transcriptase polymerase chain reaction for genes that showed changes of more than two-fold or less than half. Of the 24,546 genes, 109 genes were up-regulated greater than two-fold (e.g., FOSB, THBS1, BHLHB2, EDN1, IL11, FN1, COL10A1, and TUFT1) and 69 genes were down-regulated below 50% (e.g., SMAD6 and DCN). These results suggest that MTA, rather than being a bio-inert material, may have potential to affect the proliferation and differentiation of pulp cells in various ways.

Citations

Citations to this article as recorded by

Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells
Min-Seock Seo, Kyung-Gyun Hwang, Hyongbum Kim, Seung-Ho Baek
Restorative Dentistry & Endodontics.2012; 37(3): 142. CrossRef
Comparison of gene expression profiles of human dental pulp cells treated with mineral trioxide aggregate and calcium hydroxide
Yong-Beom Kim, Won-Jun Shon, Woocheol Lee, Kee-Yeon Kum, Seung-Ho Baek, Kwang-Shik Bae
Journal of Korean Academy of Conservative Dentistry.2011; 36(5): 397. CrossRef

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In vivo study on the biocompatibility of new resin-based root canal sealers: Yong-Beom Kim, Seung-Ho Baek, Kwang-Shik Bae; J Korean Acad Conserv Dent 2002;27(2):122-134. Published online March 31, 2002; DOI: https://doi.org/10.5395/JKACD.2002.27.2.122

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Effect of Different Sealers on the Cytocompatibility and Osteogenic Potential of Human Periodontal Ligament Stem Cells: An In Vitro Study
Shehabeldin Saber, Shereen Raafat, Mohamed Elashiry, Ahmed El-Banna, Edgar Schäfer
Journal of Clinical Medicine.2023; 12(6): 2344. CrossRef
Cytotoxicity of resin-based root canal sealer, adseal
Hee-Jung Kim, Seung-Ho Baek, Woo-Cheol Lee, Han-Soo Park, Kwang-Shik Bae
Journal of Korean Academy of Conservative Dentistry.2004; 29(6): 498. CrossRef

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