The aim of this study was to identify the bacteria isolated from acute endodontic lesions by cell culture and 16S rDNA sequencing. The necrotic pulpal tissue was collected from 17 infected root canals, which were diagnosed as being either an acute pulpitis or acute periapical abscess. Samples were collected aseptically from the infected pulpal tissue of the infected root canals using a barbed broach and a paper point. The cut barbed broaches and paper points were transferred to an eppendorf tube containing 500 ul of 1 X PBS. The sample solution was briefly mixed and plated onto a BHI-agar plate containing 5% sheep blood. The agar plates were incubated in a 37℃ anaerobic chamber for 7 days. The bacteria growing on the agar plate were identified by 16S rRNA coding gene (rDNA) cloning and sequencing at the species level. Among the 71 colonies grown on the agar plates, 56 strains survived and were identified. In dental caries involving the root canals, Streptococcus spp. were mainly isolated. Actinomyces, Clostridia, Bacteroides and Fusobacteria were isolated in the periapical lesion without dental caries. Interestingly, two new Actinomyces spp. (ChDC B639 and ChDC B631) were isolated in this study. These results showed that there was diversity among the species in endodontic lesions. This suggests that an endodontic infection is a mixed infection with a polymicrobial etiology. These results may offer the bacterial strains for pathogenesis studies related to an endodontic infection.

Citations

Citations to this article as recorded by  

• Microorganism penetration in dentinal tubules of instrumented and retreated root canal walls.In vitroSEM study
  Saad Al-Nazhan, Alaa Al-Sulaiman, Fellwa Al-Rasheed, Fatimah Alnajjar, Bander Al-Abdulwahab, Abdulhakeem Al-Badah
  Restorative Dentistry & Endodontics.2014; 39(4): 258.     CrossRef
• Microbial profile of asymptomatic and symptomatic teeth with primary endodontic infections by pyrosequencing
  Sang-Min Lim, Tae-Kwon Lee, Eun-Jeong Kim, Jun-Hong Park, Yoon Lee, Kwang-Shik Bae, Kee-Yeon Kum
  Journal of Korean Academy of Conservative Dentistry.2011; 36(6): 498.     CrossRef

Citations

Citations to this article as recorded by  

• Nanoleakage of apical sealing using a calcium silicate-based sealer according to canal drying methods
  Yoon-Joo Lee, Kyung-Mo Cho, Se-Hee Park, Yoon Lee, Jin-Woo Kim
  Restorative Dentistry & Endodontics.2024;[Epub]     CrossRef
• Comparative Evaluation of Apical Sealing Ability of Different Obturation Techniques by Confocal Microscopy: An In Vitro Study
  Reetu Arora, Yogesh Kumar, Neetu Jindal, Renu Aggarwal, Kavneet Takhar
  Dental Journal of Advance Studies.2020; 8(02): 55.     CrossRef
• Prognostic factors influencing clinical outcome of nonsurgical endodontic treatment
  Seonah Kim
  Journal of Korean Academy of Conservative Dentistry.2010; 35(6): 436.     CrossRef
• Microleakage of resilon by methacrylate-based sealer and self-adhesive resin cement
  Sun-Young Ham, Jin-Woo Kim, Hye-Jin Shin, Kyung-Mo Cho, Se-Hee Park
  Journal of Korean Academy of Conservative Dentistry.2008; 33(3): 204.     CrossRef