Skip Navigation
Skip to contents

Restor Dent Endod : Restorative Dentistry & Endodontics

OPEN ACCESS

Author index

Page Path
HOME > Browse articles > Author index
Search
Somayyeh Dehghan 1 Article
In vitro cytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assay
Sedigheh Khedmat, Somayyeh Dehghan, Jamshid Hadjati, Farimah Masoumi, Mohammad Hossein Nekoofar, Paul Michael Howell Dummer
Restor Dent Endod 2014;39(3):149-154.   Published online April 30, 2014
DOI: https://doi.org/10.5395/rde.2014.39.3.149
AbstractAbstract PDFPubReaderePub
Objectives

This study was performed to evaluate the cytotoxicity of four calcium silicate-based endodontic cements at different storage times after mixing.

Materials and Methods

Capillary tubes were filled with Biodentine (Septodont), Calcium Enriched Mixture (CEM cement, BioniqueDent), Tech Biosealer Endo (Tech Biosealer) and ProRoot MTA (Dentsply Tulsa Dental). Empty tubes and tubes containing Dycal were used as negative and positive control groups respectively. Filled capillary tubes were kept in 0.2 mL microtubes and incubated at 37℃. Each material was divided into 3 groups for testing at intervals of 24 hr, 7 day and 28 day after mixing. Human monocytes were isolated from peripheral blood mononuclear cells and cocultered with 24 hr, 7 day and 28 day samples of different materials for 24 and 48 hr. Cell viability was evaluated using an MTT assay.

Results

In all groups, the viability of monocytes significantly improved with increasing storage time regardless of the incubation time (p < 0.001). After 24 hr of incubation, there was no significant difference between the materials regarding monocyte viability. However, at 48 hr of incubation, ProRoot MTA and Biodentine were less cytotoxic than CEM cement and Biosealer (p < 0.01).

Conclusions

Biodentine and ProRoot MTA had similar biocompatibility. Mixing ProRoot MTA with PBS in place of distilled water had no effect on its biocompatibility. Biosealer and CEM cement after 48 hr of incubation were significantly more cytotoxic to on monocyte cells compared to ProRoot MTA and Biodentine.

Citations

Citations to this article as recorded by  
  • Comprehensive review of composition, properties, clinical applications, and future perspectives of calcium-enriched mixture (CEM) cement: a systematic analysis
    Saeed Asgary, Mahtab Aram, Mahta Fazlyab
    BioMedical Engineering OnLine.2024;[Epub]     CrossRef
  • Biocompatibility of mineral trioxide aggregate and biodentine as root-end filling materials: an in vivo study
    Mohamed Nabeel, Ashraf M. Abu-Seida, Abeer A. Elgendy, Hossam M. Tawfik
    Scientific Reports.2024;[Epub]     CrossRef
  • Apoptotic effects of biodentine, calcium-enriched mixture (CEM) cement, ferric sulfate, and mineral trioxide aggregate (MTA) on human mesenchymal stem cells isolated from the human pulp of exfoliated deciduous teeth
    Bahareh NAZEMI SALMAN, Mahshid MOHEBBI RAD, Ehsan SABURI
    Minerva Dental and Oral Science.2024;[Epub]     CrossRef
  • Evaluation of the Effects of Retro-Cavity Preconditioning with or Without Ethylenediaminetetraacetic Acid on Root Surface pH and Dislodgement Resistance of NeoMTA2 and Mineral Trioxide Aggregate Flow Retro-Fills: An Ex Vivo Investigation
    Sedigheh Khedmat, Seyyed Ali Abaee, Hadi Assadian, Antonio Signore, Stefano Benedicenti
    Journal of Functional Biomaterials.2024; 16(1): 3.     CrossRef
  • Bone morphogenetic proteins in biomineralization of two endodontic restorative cements
    Tamara A. Souza, Mirna M. Bezerra, Paulo G. B. Silva, José J. N. Costa, Rayssa F. L. A. Carneiro, Janice O. F. Barcelos, Bruno C. Vasconcelos, Hellíada V. Chaves
    Journal of Biomedical Materials Research Part B: Applied Biomaterials.2021; 109(3): 348.     CrossRef
  • Cytotoxicity and Bioactivity of Mineral Trioxide Aggregate and Bioactive Endodontic Type Cements: A Systematic Review
    Uma Dixit, Rucha Shivajirao Bhise Patil, Rupanshi Parekh
    International Journal of Clinical Pediatric Dentistry.2021; 14(1): 30.     CrossRef
  • Physicochemical and Biological Properties of Mg-Doped Calcium Silicate Endodontic Cement
    Kyung-Hyeon Yoo, Yong-Il Kim, Seog-Young Yoon
    Materials.2021; 14(8): 1843.     CrossRef
  • Comparative evaluation of the effect of cold ceramic and MTA-Angelus on cell viability, attachment and differentiation of dental pulp stem cells and periodontal ligament fibroblasts: an in vitro study
    Sedigheh Khedmat, Pegah Sarraf, Ehsan Seyedjafari, Parisa Sanaei-rad, Faranak Noori
    BMC Oral Health.2021;[Epub]     CrossRef
  • Cytotoxicity of universal dental adhesive systems: Assessment in vitro assays on human gingival fibroblasts
    Stefano Pagano, Guido Lombardo, Stefania Balloni, Maria Bodo, Stefano Cianetti, Antonella Barbati, Azadeh Montaseri, Lorella Marinucci
    Toxicology in Vitro.2019; 60: 252.     CrossRef
  • The effect of desiccation on water sorption, solubility and hygroscopic volumetric expansion of dentine replacement materials
    Ruba Mustafa, Ruwaida Z. Alshali, Nick Silikas
    Dental Materials.2018; 34(8): e205.     CrossRef
  • Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0℃/2 MPa Condition up to One Week: In Vivo MTT Method
    Sun Mi Jang, Sin-Yeon Cho, Eui-Seong Kim, Il-Young Jung, Seung Jong Lee
    Journal of Korean Dental Science.2016; 9(1): 1.     CrossRef
  • Cytotoxic effects of one‐step self‐etching adhesives on an odontoblast cell line
    Yoon Lee, So‐Youn An, Yoon‐Jung Park, Frank H. Yu, Joo‐Cheol Park, Deog‐Gyu Seo
    Scanning.2016; 38(1): 36.     CrossRef
  • Influence of Biodentine® - A Dentine Substitute - On Collagen Type I Synthesis in Pulp Fibroblasts In Vitro
    Frangis Nikfarjam, Kim Beyer, Anke König, Matthias Hofmann, Manuel Butting, Eva Valesky, Stefan Kippenberger, Roland Kaufmann, Detlef Heidemann, August Bernd, Nadja Nicole Zöller, Dimitrios Karamichos
    PLOS ONE.2016; 11(12): e0167633.     CrossRef
  • Evaluation of cytotoxicity and gelatinases activity in 3T3 fibroblast cell by root repair materials
    Varol Basak, Tuna Elif Bahar, Karsli Emine, Kasimoglu Yelda, Koruyucu Mine, Seymen Figen, Nurten Rustem
    Biotechnology & Biotechnological Equipment.2016; 30(5): 984.     CrossRef
  • In VitroCytotoxicity of Calcium Silicate-Based Endodontic Cement as Root-End Filling Materials
    Selen Küçükkaya, Mehmet Ömer Görduysus, Naciye Dilara Zeybek, Sevda Fatma Müftüoğlu
    Scientifica.2016; 2016: 1.     CrossRef
  • Cytotoxic effects of mineral trioxide aggregate, calcium enrichedmixture cement, Biodentine and octacalcium pohosphate onhuman gingival fibroblasts
    Eshagh A. Saberi, Narges Farhadmollashahi, Faroogh Ghotbi, Hamed Karkeabadi, Roholla Havaei
    Journal of Dental Research, Dental Clinics, Dental Prospects.2016; 10(2): 75.     CrossRef
  • Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials
    Diana María Escobar-García, Eva Aguirre-López, Verónica Méndez-González, Amaury Pozos-Guillén
    BioMed Research International.2016; 2016: 1.     CrossRef
  • Cytotoxicity and osteogenic potential of silicate calcium cements as potential protective materials for pulpal revascularization
    Eduardo A. Bortoluzzi, Li-na Niu, Chithra D. Palani, Ahmed R. El-Awady, Barry D. Hammond, Dan-dan Pei, Fu-cong Tian, Christopher W. Cutler, David H. Pashley, Franklin R. Tay
    Dental Materials.2015; 31(12): 1510.     CrossRef
  • 51 View
  • 0 Download
  • 18 Crossref
Close layer

Restor Dent Endod : Restorative Dentistry & Endodontics
Close layer
TOP