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In vitro cytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assay
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Sedigheh Khedmat, Somayyeh Dehghan, Jamshid Hadjati, Farimah Masoumi, Mohammad Hossein Nekoofar, Paul Michael Howell Dummer
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Restor Dent Endod 2014;39(3):149-154. Published online April 30, 2014
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DOI: https://doi.org/10.5395/rde.2014.39.3.149
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 Abstract
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- Objectives
This study was performed to evaluate the cytotoxicity of four calcium silicate-based endodontic cements at different storage times after mixing. Materials and MethodsCapillary tubes were filled with Biodentine (Septodont), Calcium Enriched Mixture (CEM cement, BioniqueDent), Tech Biosealer Endo (Tech Biosealer) and ProRoot MTA (Dentsply Tulsa Dental). Empty tubes and tubes containing Dycal were used as negative and positive control groups respectively. Filled capillary tubes were kept in 0.2 mL microtubes and incubated at 37℃. Each material was divided into 3 groups for testing at intervals of 24 hr, 7 day and 28 day after mixing. Human monocytes were isolated from peripheral blood mononuclear cells and cocultered with 24 hr, 7 day and 28 day samples of different materials for 24 and 48 hr. Cell viability was evaluated using an MTT assay. ResultsIn all groups, the viability of monocytes significantly improved with increasing storage time regardless of the incubation time (p < 0.001). After 24 hr of incubation, there was no significant difference between the materials regarding monocyte viability. However, at 48 hr of incubation, ProRoot MTA and Biodentine were less cytotoxic than CEM cement and Biosealer (p < 0.01). ConclusionsBiodentine and ProRoot MTA had similar biocompatibility. Mixing ProRoot MTA with PBS in place of distilled water had no effect on its biocompatibility. Biosealer and CEM cement after 48 hr of incubation were significantly more cytotoxic to on monocyte cells compared to ProRoot MTA and Biodentine.
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Citations
Citations to this article as recorded by  - Comprehensive review of composition, properties, clinical applications, and future perspectives of calcium-enriched mixture (CEM) cement: a systematic analysis
Saeed Asgary, Mahtab Aram, Mahta Fazlyab
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Sedigheh Khedmat, Seyyed Ali Abaee, Hadi Assadian, Antonio Signore, Stefano Benedicenti
Journal of Functional Biomaterials.2024; 16(1): 3. CrossRef - Bone morphogenetic proteins in biomineralization of two endodontic restorative cements
Tamara A. Souza, Mirna M. Bezerra, Paulo G. B. Silva, José J. N. Costa, Rayssa F. L. A. Carneiro, Janice O. F. Barcelos, Bruno C. Vasconcelos, Hellíada V. Chaves
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Uma Dixit, Rucha Shivajirao Bhise Patil, Rupanshi Parekh
International Journal of Clinical Pediatric Dentistry.2021; 14(1): 30. CrossRef - Physicochemical and Biological Properties of Mg-Doped Calcium Silicate Endodontic Cement
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Frangis Nikfarjam, Kim Beyer, Anke König, Matthias Hofmann, Manuel Butting, Eva Valesky, Stefan Kippenberger, Roland Kaufmann, Detlef Heidemann, August Bernd, Nadja Nicole Zöller, Dimitrios Karamichos
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Eduardo A. Bortoluzzi, Li-na Niu, Chithra D. Palani, Ahmed R. El-Awady, Barry D. Hammond, Dan-dan Pei, Fu-cong Tian, Christopher W. Cutler, David H. Pashley, Franklin R. Tay
Dental Materials.2015; 31(12): 1510. CrossRef
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Evaluation of the effect of blood contamination on the compressive strength of MTA modified with hydration accelerators
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Kaveh Oloomi, Eshaghali Saberi, Hadi Mokhtari, Hamid Reza Mokhtari Zonouzi, Ali Nosrat, Mohammad Hossein Nekoofar, Paul Michael Howell Dummer
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Restor Dent Endod 2013;38(3):128-133. Published online August 23, 2013
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DOI: https://doi.org/10.5395/rde.2013.38.3.128
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Abstract
PDFPubReaderePub
- Objectives
This study was performed to evaluate the effect of blood contamination on the compressive strength (CS) of Root MTA (RMTA) modified with Calcium chloride (CaCl2) and Disodium hydrogen phosphate (Na2HPO4) as setting accelerators over time. Materials and MethodsA total of 110 cylindrical specimens of RMTA were divided into 6 experimental groups as follows: Group1, RMTA; Group 2, RMTA modified with CaCl2 (RMTA-C); Group 3, RMTA modified with Na2HPO4 (RMTA-N); Group 4, RMTA contaminated with blood; Group 5, RMTA-C contaminated with blood; Group 6, RMTA-N contaminated with blood. The CS of specimens in all groups was evaluated after 3 hr, 24 hr, and 1 wk. In the modified groups (groups 2, 3, 5, and 6) the CS of five specimens per group was also evaluated after 1 hr. ResultsBlood contamination significantly reduced the CS of all materials at all time intervals (p < 0.05). After 3 hr, the CS of specimens in the RMTA groups (with and without blood contamination) was significantly lower than those in the RMTA-C and RMTA-N groups (p < 0.05). The CS values were not significantly different at the other time intervals. In all groups, the CS of specimens significantly increased over time (p < 0.05). ConclusionsBlood contamination decreased the CS of both original and accelerated RMTA.
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Citations
Citations to this article as recorded by - The effect of three additives on properties of mineral trioxide aggregate cements: a systematic review and meta-analysis of in vitro studies
Behnam Bolhari, Faranak Noori, Hadi Assadian, Amir Raee, Sholeh Ghabraei, Ahmad-Reza Shamshiri, Artak Heboyan
BMC Oral Health.2024;[Epub] CrossRef - Push‐out bond strength of the calcium silicate‐based endodontic cements in the presence of blood: A systematic review and meta‐analysis of in vitro studies
Mahdieh Alipour, Leili Faraji Gavgani, Negin Ghasemi
Clinical and Experimental Dental Research.2022; 8(2): 571. CrossRef - Effect of bioactive glass addition on the physical properties of mineral trioxide aggregate
Jei Kim, Hyun-Jung Kim, Seok Woo Chang, Soram Oh, Sun-Young Kim, Kyoung-Kyu Choi, Duck-Su Kim, Ji-Hyun Jang
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Cleonice da Silveira Teixeira, Jessica Coelho Wasielewsky, Giovanna Slongo dos Santos, Anarela Bernardi, Eduardo Antunes Bortoluzzi, Lucas da Fonseca Roberti Garcia
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Muzaffer Emir Dinçol, Hakan Ozbas, Bulent Yılmaz, Handan Ersev, Selcuk Gokyay, Vakur Olgac
BMC Oral Health.2016;[Epub] CrossRef - Surface microhardness of three thicknesses of mineral trioxide aggregate in different setting conditions
Noushin Shokouhinejad, Leila Jafargholizadeh, Mehrfam Khoshkhounejad, Mohammad Hossein Nekoofar, Maryam Raoof
Restorative Dentistry & Endodontics.2014; 39(4): 253. CrossRef - Surgical management of a failed internal root resorption treatment: a histological and clinical report
Saeed Asgary, Mohammad Jafar Eghbal, Leili Mehrdad, Sanam Kheirieh, Ali Nosrat
Restorative Dentistry & Endodontics.2014; 39(2): 137. CrossRef
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