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Elemental analysis of caries-affected root dentin and artificially demineralized dentin
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Young-Hye Sung, Ho-Hyun Son, Keewook Yi, Juhea Chang
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Restor Dent Endod 2016;41(4):255-261. Published online August 19, 2016
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DOI: https://doi.org/10.5395/rde.2016.41.4.255
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Abstract
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- Objectives
This study aimed to analyze the mineral composition of naturally- and artificially-produced caries-affected root dentin and to determine the elemental incorporation of resin-modified glass ionomer (RMGI) into the demineralized dentin. Materials and MethodsBox-formed cavities were prepared on buccal and lingual root surfaces of sound human premolars (n = 15). One cavity was exposed to a microbial caries model using a strain of Streptococcus mutans. The other cavity was subjected to a chemical model under pH cycling. Premolars and molars with root surface caries were used as a natural caries model (n = 15). Outer caries lesion was removed using a carbide bur and a hand excavator under a dyeing technique and restored with RMGI (FujiII LC, GC Corp.). The weight percentages of calcium (Ca), phosphate (P), and strontium (Sr) and the widths of demineralized dentin were determined by electron probe microanalysis and compared among the groups using ANOVA and Tukey test (p < 0.05). ResultsThere was a pattern of demineralization in all models, as visualized with scanning electron microscopy. Artificial models induced greater losses of Ca and P and larger widths of demineralized dentin than did a natural caries model (p < 0.05). Sr was diffused into the demineralized dentin layer from RMGI. ConclusionsBoth microbial and chemical caries models produced similar patterns of mineral composition on the caries-affected dentin. However, the artificial lesions had a relatively larger extent of demineralization than did the natural lesions. RMGI was incorporated into the superficial layer of the caries-affected dentin.
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Citations
Citations to this article as recorded by 
- Frontiers of Global Research Trend on Root Caries: A Bibliometric Analysis
Grace Yuchan Xu, Irene Shuping Zhao, Christie Ying Kei Lung, Iris Xiaoxue Yin, Edward Chin Man Lo, Chun Hung Chu International Dental Journal.2024; 74(6): 1197. CrossRef - Effects of NaF versus SDF treatment on microhardness of artificial radiation caries at cervical and root areas
Pipop SAIKAEW, Karis KATEKOVIT, Anocha BURANARACHADA, Nattapat SAIMALA, Anussara PRAYONGRAT, Pornpoj FUANGTHARNTHIP Dental Materials Journal.2024; 43(4): 591. CrossRef - Degradable polycaprolactone/buffer composites as pH regulating carrier materials for drug delivery and 3D printed biomaterials
Therese Schüler, Celine Guder, Franziska Alt, Katrin Lorenz, Torsten Sterzenbach, Christian Hannig, Hans-Peter Wiesmann, Benjamin Kruppke Materialia.2024; 34: 102087. CrossRef - The Effect of Oral Care Foams and a Spray on Salivary pH Changes after Exposure to Acidic Beverages in Young Adults
Maria Polyakova, Anna Egiazaryan, Vladlena Doroshina, Alexandr Zaytsev, Alexey Malashin, Ksenia Babina, Nina Novozhilova Dentistry Journal.2024; 12(4): 93. CrossRef - In Vitro Models Used in the Formation of Root Caries Lesions—A Review of the Literature
Zaid Dohan, Lara T. Friedlander, Paul R. Cooper, Kai-Chun Li, Jithendra T. Ratnayake, May L. Mei Dentistry Journal.2023; 11(12): 269. CrossRef - Yttrium Trifluoride as a Marker of Infiltration Rate of Decalcified Root Cementum: An In Vitro Study
Anna Nowak-Wachol, Anna Korytkowska-Wałach, Bartosz Chmiela, Kacper Wachol, Maciej Łopaciński, Magdalena Wyszyńska, Yousuf Al-Dulaimi, Małgorzata Skucha-Nowak Polymers.2022; 14(4): 780. CrossRef - Effect of fluoride, chlorhexidine or Nd:YAG on the progression of root dentin demineralization after removal of the demineralized organic matrix
Andrea Maselli, Tânia Mara da Silva, Lucélia Lemes Gonçalves, Aline Silva Braga, Eduardo Bresciani, Ana Carolina Magalhães, Sérgio Eduardo de Paiva Gonçalves Journal of Applied Oral Science.2022;[Epub] CrossRef - Effect of ionizing radiation and cariogenic biofilm challenge on root-dentin caries
Camila de Carvalho Almança Lopes, Renata Borges Rodrigues, Maximiliano Sérgio Cenci, Juliana Lays Stolfo Uehara, Tamires Timm Maske, Pedro Henrique Justino Oliveira Limirio, Priscilla Barbosa Ferreira Soares, Veridiana Resende Novais Clinical Oral Investigations.2021; 25(6): 4059. CrossRef - Silver diamine fluoride treatment of active root caries lesions in older adults: A case series
Chelsea Mitchell, Andrew J Gross, Peter Milgrom, Lloyd Mancl, David B Prince Journal of Dentistry.2021; 105: 103561. CrossRef - A Hydrogel Drink With High Fructose Content Generates Higher Exogenous Carbohydrate Oxidation and a Reduced Drop in Dental Biofilm pH Compared to Two Other, Commercially Available, Carbohydrate Sports Drinks
Stefan Pettersson, Martin Ahnoff, Fredrik Edin, Peter Lingström, Charlotte Simark Mattsson, Ulrika Andersson-Hall Frontiers in Nutrition.2020;[Epub] CrossRef
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Elemental analysis of the fluoride varnish effects on root caries initiation
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Se-Eun Park, Keewook Yi, Hae-Young Kim, Ho-Hyun Son, Juhea Chang
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J Korean Acad Conserv Dent 2011;36(4):290-299. Published online July 31, 2011
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DOI: https://doi.org/10.5395/JKACD.2011.36.4.290
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Abstract
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Objectives
The usage of fluoride varnish for a moderate to low caries-risk group has not been well validated. This study aimed to evaluate the preventive and therapeutic efficacies of fluoride varnish on the initiated root caries.
Materials and Methods
Ten premolars were sectioned into quarters, further divided into two windows, one of which was painted with Fluor Protector (1,000 ppm fluoride, Ivoclar Vivadent). An initial lesion with a well-preserved surface layer was produced by pH cycling. Scanned line analysis using energy dispersive spectrometry determined the weight percentages of Ca and P in the demineralized layer. Scanning Electron microscopy and confocal laser scanning microscopy (CLSM) evaluated the varnish-applied root surfaces.
Results
The mean lesion depth (SD) was 12.3 (2.6) µm (single cycling) and 19.6 (3.8) µm (double cycling). Double cycling extended the lesion depth, but induced no more mineral loss than single cycling (p < 0.05). The mean weight percentages of Ca and P between groups with and without varnish were not significantly different (p < 0.05). A CLSM showed varnish remained within 15 µm of the surface layer.
Conclusions
When a mild acid challenge initiated root tissue demineralization, the application of low-concentration fluoride varnish did not influence the lesion depth or the mineral composition of the subsurface lesion.
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Citations
Citations to this article as recorded by 
- The combined occluding effect of sodium fluoride varnish and Nd:YAG laser irradiation on dentinal tubules—A CLSM and SEM study
Samet Tosun, Emre Culha, Ugur Aydin, Abdul Semih Ozsevik Scanning.2016; 38(6): 619. CrossRef - How to designin situstudies: an evaluation of experimental protocols
Young-Hye Sung, Hae-Young Kim, Ho-Hyun Son, Juhea Chang Restorative Dentistry & Endodontics.2014; 39(3): 164. CrossRef - Evaluation of release of fluoride from dental varnishes marketed in Korea
Han-Na Kim, Myung-Su Jeong, Se-Yeon Kim, Jin-Bom Kim, Seung-Hwa Jeong Journal of Korean Academy of Oral Health.2014; 38(3): 131. CrossRef
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Quantitative comparison of permeability in the adhesive interface of four adhesive systems
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Juhea Chang, Keewook Yi, Hae-Young Kim, In Bog Lee, Byeong Hoon Cho, Ho-Hyun Son
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J Korean Acad Conserv Dent 2009;34(1):51-60. Published online January 31, 2009
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DOI: https://doi.org/10.5395/JKACD.2009.34.1.051
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Abstract
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The purpose of this study was to perform quantitative comparisons of water permeable zones in both the adhesive and the hybrid layer before and after thermocycling in order to assess the integrity of the bonding interface. Twenty eight flat dentin surfaces were bonded with a light-cured composite resin using one of four commercial adhesives [OptiBond FL (OP), AdheSE (AD), Clearfil SE Bond (CL), and Xeno III (XE)]. These were sectioned into halves and subsequently cut to yield 2-mm thick specimens; one specimen for control and the other subjected to thermocycling for 10,000 cycles. After specimens were immersed in ammoniacal silver nitrate for 24 h and exposed to a photo developing solution for 8 h, the bonded interface was analyzed by scanning electron microscopy (SEM) and wavelength dispersive spectrometry (WDS) at five locations per specimen. Immediately after bonding, the adhesive layer of OP showed the lowest silver uptake, followed by CL, AD, and XE in ascending order (p < 0.0001); the hybrid layer of CL had the lowest silver content among the groups (p = 0.0039). After thermocycling, none of the adhesives manifested a significant increase of silver in either the adhesive or the hybrid layer. SEM demonstrated the characteristic silver penetrated patterns within the interface. It was observed that integrity of bonding was well maintained in OP and CL throughout the thermocycling process. Adhesive-tooth interfaces are vulnerable to hydrolytic degradation and its permeability varies in different adhesive systems, which may be clinically related to the restoration longevity.
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