This study investigated the latest findings and notions regarding ‘triple antibiotic paste’ (TAP) and its applications in dentistry, particularly endodontics. TAP is a combination of 3 antibiotics, ciprofloxacin, metronidazole, and minocycline. Despite the problems and pitfalls research pertaining to this paste has unveiled, it has been vastly used in endodontic treatments. The paste's applications vary, from vital pulp therapy to the recently introduced regeneration and revascularisation protocol. Studies have shown that the paste can eliminate the root canal microorganisms and prepare an appropriate matrix for further treatments. This combination is able to remove diverse groups of obligate and facultative gram-positive and gram-negative bacteria, providing an environment for healing. In regeneration protocol cases, this allows the development, disinfection, and possible sterilization of the root canal system, so that new tissue can infiltrate and grow into the radicular area. Moreover, TAP is capable of creating a discipline in which other wanted and needed treatments can be successfully performed. In conclusion, TAP, as an antibacterial intracanal medication, has diverse uses. Nevertheless, despite its positive effects, the paste has shown drawbacks. Further research concerning the combined paste and other intracanal medications to control microbiota is a must.

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• Evaluation of Effect of Laser in Root Canal Disinfection in Pulp Regenerative Therapy: A Systematic Review
  Kiran Kumar N, Abhishek M, Savitha B. Naik, Biji Brigit, Swetha Geervani V, M Manimozhi
  Photobiomodulation, Photomedicine, and Laser Surgery.2025; 43(2): 53.     CrossRef
• Assessing Cell Viability: Comparative Analysis of Calcium Hydroxide, Triple Antibiotic Paste, and Their Synergistic Impact on human Dental Pulp Stem Cells
  Dini Asrianti Bagio, Ibramanto Warganegara, Ike Dwi Maharti, Anggraini Margono, Citra Kusumasari, Sylva Dinie Alinda, Valeria Widita Wairooy
  European Journal of General Dentistry.2025; 14(01): 073.     CrossRef
• Cytotoxic and Antibiofilm Properties of Antibiotic-Loaded Thermoresponsive Hydrogels for Root Canal Therapy
  Cristiane Duque, Gabriela Pacheco de Almeida Braga, Juliana Machado de Carvalho, Karina Sampaio Caiaffa, Gabriel Pereira Nunes, Rafaela Laruzo Rabelo, Vanessa Rodrigues dos Santos, Geórgia Rondó Peres, Lucas da Silva Ribeiro, Emerson Rodrigues de Camargo
  Processes.2025; 13(3): 661.     CrossRef
• Antibiofilm properties, cytotoxicity, and effect on protease activity of antibiotics and EGCG-based medications for endodontic purposes
  Daniela Alvim Chrisostomo, Jesse Augusto Pereira, Polliana Mendes Candia Scaffa, Zach Gouveia, Gabriel Flores Abuna, Sergey Plotnikov, Anuradha Prakki, Cristiane Duque
  Journal of Dentistry.2025; : 105660.     CrossRef
• A Comparative Evaluation of Herbal Extracts and Triple Antibiotic Paste as Intracanal Medicament against Enterococcus faecalis: A Microbiological Study
  Divya Singh, Rashi Singh, Nidhi Gupta, Natasha Gambhir, Saritha Golla
  International Journal of Clinical Pediatric Dentistry.2024; 17(3): 285.     CrossRef
• Comparative Evaluation of Push-Out Bond Strength of Conventional Mineral Trioxide Aggregate, Biodentine, a Modified Mineral Trioxide Aggregate, and Two Novel Antibacterial-Enhanced Mineral Trioxide Aggregates
  Arokia Rajkumar Shancy Merlin, Vignesh Ravindran, Ganesh Jeevanandan, Rajalakshmanan Eswaramoorthy, Abirami Arthanari
  Cureus.2024;[Epub]     CrossRef
• In Vitro Evaluation of the Effect of Oleanolic Acid as a Potential Root Canal Medicament on Viability and Proliferation of Dental Pulp Stem Cells
  Khalifah A. Alhaila, Manal Farouk Badawi, Mohamed G. Elbeltagy, Amany E. Badr
  European Journal of General Dentistry.2024; 13(01): 051.     CrossRef
• Regenerative Endodontic Treatment in Dentinogenesis Imperfecta‐Induced Apical Periodontitis
  Ying Liao, Ting Pan, Xianghui Xing, Sivakumar Nuvvula
  Case Reports in Dentistry.2024;[Epub]     CrossRef
• Efecto antimicrobiano como medicación intraconducto de la pasta triantibiótica.
  Paúl Sebastián Ulloa Amores, Diana Álvarez Álvarez, María Elizabeth Moscoso Abad, Magda Zulay Bastidas Calva
  Revista de la Asociación Dental Mexicana.2024; 81(4): 211.     CrossRef
• Synthesis and Characterization of Nanosilica-Coated Antibiotics, TAP: A Comprehensive Study Utilizing XRD, EDS, FTIR, SEM, and TEM – Invitro Study
  Mahaboob S. Hameed, S. Delphine P. Antony, Rajeshkumar Shanmugam, Sandhya Raghu
  Journal of International Oral Health.2024; 16(5): 386.     CrossRef
• Association between host defence peptide IDR‐1002 and ciprofloxacin: Effects on human dental pulp cells
  Danilo César Mota Martins, Maurício Gonçalves da Costa Sousa, Poliana Amanda Oliveira Silva, Lana Ribeiro Aguiar, Rosângela Vieira de Andrade, Amandda Évellin Silva‐Carvalho, Felipe Saldanha‐Araújo, Octávio Luiz Franco, Taia Maria Berto Rezende
  Australian Endodontic Journal.2024; 50(3): 547.     CrossRef
• Comparative evaluation of antimicrobial efficacy of triple antibiotic paste and amoxicillin clavulanate paste as an intracanal medicament against Enterococcus faecalis: An in vitro study
  Dhandayuthapani Sasikala, Parisa Norouzi Baghkomeh, Jamaluddin Mohammed Farzan
  Dental Research Journal.2024;[Epub]     CrossRef
• Triple Antibiotic Paste: A Game Changer in Endodontics
  Kalagi G. Panchal, Karima Virani, Vraj Patel, Aquib Ali Khan, Anam Pettiwala, Srikala S. Puranik, Srushti Joshi
  Journal of Pharmacy and Bioallied Sciences.2024; 16(Suppl 3): S1913.     CrossRef
• Non-surgical Management of a Large Periapical Lesion: A Case Study of the Successful Application of a Modified Triple Antibacterial Paste
  Srushti Awghad, Joyeeta Mahapatra, Amit Reche, Ankita Burse, Aradhana Kibe
  Cureus.2024;[Epub]     CrossRef
• Enhancing Antimicrobial Efficacy and Synergistic Effects of Nano-Silica-Based Combinations With Doxycycline, Metronidazole, and Ciprofloxacin Against Enterococcus faecalis Biofilms
  Shahul Hameed, Delphine P Antony, Rajeshkumar Shanmugam, Sandhya Raghu, Hima Sandeep Adimulapu
  Cureus.2024;[Epub]     CrossRef
• Healing of periapical lesion by non-surgical endodontic therapy: A case series
  Athira Ramesh, Rajesh Pillai, Afzal A, Anakha Santhosh, Arunima G.S, Sandeep K. V
  IP Indian Journal of Conservative and Endodontics.2024; 9(2): 99.     CrossRef
• Effect of Calcium Hydroxide Versus Double Antibiotic Paste on Endodontic Treatment Outcomes in Teeth With Large Periapical Lesions: A Triple‐Blind Randomized Clinical Trial
  Afsaneh Rahmati, Farshad Seyedein, Omid Dianat, Sara Saedi, Golriz Rostami, Alireza Akbarzadeh Baghban, Shima Sabertahan, Majid Kazem, Kee Y. Kum
  International Journal of Dentistry.2024;[Epub]     CrossRef
• Can antimicrobial photodynamic therapy serve as an effective adjunct protocol for disinfecting the necrotic root canal system? A randomized controlled study
  Remy Barazy, Hisham Alafif, Hassan Achour, Ahmad Al-Aloul, Yasser Alsayed Tolibah
  BDJ Open.2024;[Epub]     CrossRef
• Drain and Treat: A Rubber Dam Technique for Acute Periapical Abscess
  S. Grover, K. Mala, J. D’Souza
  Acta Medica Bulgarica.2024; 51(s2): 143.     CrossRef
• Microbial Dynamics in Endodontic Pathology—From Bacterial Infection to Therapeutic Interventions—A Narrative Review
  Klara Wieczorkiewicz, Anna Jarząbek, Estera Bakinowska, Kajetan Kiełbowski, Andrzej Pawlik
  Pathogens.2024; 14(1): 12.     CrossRef
• An in vitro comparative evaluation of the effect of three intracanal medicaments – chlorhexidine gel, triple antibiotic paste, and calcium hydroxide paste on the push-out bond strength of MTA Plus, Biodentine, and calcium-enriched mixture
  Gouthami Datta, Ramya Raghu, Ashish Shetty, Gautham P Manjunath, Dishant Patel, Subhashini Rajasekhara
  Endodontology.2023; 35(1): 60.     CrossRef
• The cytotoxic effect of cysteamine and its combinations with various endodontic intracanal medications on fibroblast cells: in vitro study
  Esraa Adel Mohamed Abd Elhameed ElGammal, Abeer Hashem Mahran, Salma Hassan El Ashry, Sara Hossam Fahmy
  Bulletin of the National Research Centre.2023;[Epub]     CrossRef
• Healing of large endodontic lesions using a new combination of triple antibiotics: A case report
  Saeed Asgary, Ardavan Parhizkar
  Clinical Case Reports.2023;[Epub]     CrossRef
• Effects of Intracanal Medicaments on the Measurement Accuracy of Four Apex Locators: An In Vitro Study
  Hamza Cudal, Tuğrul Aslan, Bertan Kesim
  Meandros Medical and Dental Journal.2023; 24(3): 215.     CrossRef
• Comparative evaluation of the antibacterial efficacy of herbal agents as intracanal medicaments individually or in combination with chitosan: An in vitro RTPCR study
  Gaurav Patri, Kotni Sheetal, PrasantiKumar Pradhan, Pratik Agrawal, S Lata
  Journal of International Oral Health.2023; 15(1): 89.     CrossRef
• Recent progress in carbon dots for anti-pathogen applications in oral cavity
  Yuying Jiang, Chuqiang Yin, Jianning Mo, Xiaoyu Wang, Ting Wang, Guotai Li, Qihui Zhou
  Frontiers in Cellular and Infection Microbiology.2023;[Epub]     CrossRef
• A Comprehensive Review of Lesion Sterilization and Tissue Repair: An Alternative for Pulpectomy in Deciduous Teeth
  Aparna Achanta, Amit Reche, Rishika Dakhale, Rudra R Bharate
  Cureus.2023;[Epub]     CrossRef
• Healing of Large Endodontic Lesions Using Long-Term Application of a New Combination of Triple Antibiotics: A Series of Cases
  Saeed Asgary, Ardavan Parhizkar, Maria Beatriz Duarte Gavião
  Case Reports in Dentistry.2023; 2023: 1.     CrossRef
• Non-cytotoxic Root Canal Dressing with Improved Antimicrobial Efficacy
  Farzad Koosha, Jerome Cymerman, Thomas Manders, Marcia Simon, Stephen Walker, Miriam Rafailovich
  Journal of Endodontics.2023; 49(2): 205.     CrossRef
• Triple antibiotics: A synergistic approach to combating infection
  Hemant Sawhney, Anukriti Kumari, Ritik Kashwani, Geetanjali Gupta, SJ Das
  IP Indian Journal of Conservative and Endodontics.2023; 8(4): 189.     CrossRef
• A 1-year Clinical and Radiographic Assessment of Regenerative Endodontic Therapy for Necrotic Primary Molars: A Randomized controlled Trial
  Dina D Abdelmoneim, Amr M Abdelaziz, Gehan G Allam, Amira S Badran
  International Journal of Clinical Pediatric Dentistry.2023; 16(2): 295.     CrossRef
• “BIODENTINE” THE DENTINE IN A CAPSULE AS AN APICAL BARRIER IN TRAUMATIZED MAXILLARY CENTRAL INCISOR WITH TWO YEARS FOLLOW UP.
  Savita Thakur, Udai Bhanu, Gurkirat Singh Grewal
  INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH.2022; : 64.     CrossRef
• Long-term treatment of dentine with triple antibiotic paste promotes stem cell viability and attachment
  Samiya Riaz, Ahmad Azlina, Zuliani Mahmood, Aung T. Htun
  Journal of Taibah University Medical Sciences.2022; 17(4): 630.     CrossRef
• Non surgical management of trauma induced root resorption and large periapical lesion using bioactive material- A case report
  Tejasvini Prajapati, Sonali Kapoor, Purnil Shah, Ankit Arora, Hardik Rana
  Clinical Dentistry.2022;[Epub]     CrossRef
• Antibacterial efficacy of antibiotic pastes versus calcium hydroxide intracanal dressing: A systematic review and meta-analysis of ex vivo studies
  Mohammadreza Vatankhah, Kamyar Khosravi, Nazanin Zargar, Armin Shirvani, MohammadHossein Nekoofar, Omid Dianat
  Journal of Conservative Dentistry.2022; 25(5): 463.     CrossRef
• Triple Antibiotic Paste: A Suitable Medicament for Intracanal Disinfection
  Krutika Malu, Monika Khubchandani
  Cureus.2022;[Epub]     CrossRef
• Present status and future directions of intracanal medicaments
  Ronald Ordinola‐Zapata, W. Craig Noblett, Alejandro Perez‐Ron, Zhou Ye, Jorge Vera
  International Endodontic Journal.2022; 55(S3): 613.     CrossRef
• The effect of four different intracanal medicaments on the push-out bond strength of root canal sealers
  Shalu Maan, Vijaya Dhar Bhatt, Rohit Singh, Sayak Gupta, Syed Alay Noorain, Aashna Gill, Pradeep Kumar, Sushil Yadav, Preeti Sharma
  Journal of Medicine and Life.2022; 15(4): 448.     CrossRef
• A Comparative Evaluation of Microhardness and Chemical Structure of Radicular Dentin with Two Combinations of TAP and MTAP: An In Vitro Study
  P Niharika, Saigeeta Kondamadugu, Nagireddy Venugopal Reddy, Muthumula Daneswari, Annie P Chris, Nikhila V Reddy
  International Journal of Clinical Pediatric Dentistry.2022; 15(S2): S151.     CrossRef
• Comparative Evaluation of Two Antibiotic Pastes for Root Canal Disinfection
  Sadhna Sharma, Urvashi Bhushan, Mridula Goswami, CP Baveja
  International Journal of Clinical Pediatric Dentistry.2022; 15(S1): S12.     CrossRef
• Management of External Inflammatory Root Resorption following Tooth Autotransplantation Using a Modified Combination of Triple Antibiotics
  Saeed Asgary, Ardavan Parhizkar, Paulo J. Palma
  Case Reports in Dentistry.2022; 2022: 1.     CrossRef
• Effect of triple antibiotic paste on the bond strength of epoxy and methacrylate resin-based sealers to root canal dentin
  Fereshte Sobhnamayan, Alireza Adl, Mahdi Sedigh-Shams, Hossein Mirkhaghani
  Journal of Conservative Dentistry.2022; 25(4): 426.     CrossRef
• Progress of Research on the Application of Triple Antibiotic Paste and Hydrogel Scaffold Materials in Endodontic Revascularization: A Systematic Review
  Jia Zhao, Tian Jiao Wang
  Evidence-Based Complementary and Alternative Medicine.2022; 2022: 1.     CrossRef
• The effect of different intracanal medicaments on the dislodgement resistance of mineral trioxide aggregate
  Farzaneh Afkhami, Shahrzad Razavi, Sholeh Ghabraei
  BMC Oral Health.2022;[Epub]     CrossRef
• Efficacy of Ciprofloxacin, Metronidazole and Minocycline in Ordered Mesoporous Silica against Enterococcus faecalis for Dental Pulp Revascularization: An In-Vitro Study
  Cintia Micaela Chamorro-Petronacci, Beatriz Santos Torres, Rocío Guerrero-Nieves, Mario Pérez-Sayáns, Marcia Carvalho-de Abreu Fantini, Luis Carlos Cides-da-Silva, Beatriz Magariños, Berta Rivas-Mundiña
  Materials.2022; 15(6): 2266.     CrossRef
• Antibiotic Mixtures in Noninstrumental Endodontic Treatment of Primary Teeth with Necrotic Pulps: A Systematic Review
  Farah Chouchene, Fatma Masmoudi, Ahlem Baaziz, Fethi Maatouk, Hichem Ghedira, Sivakumar Nuvvula
  International Journal of Dentistry.2021; 2021: 1.     CrossRef
• Potential apply of hydrogel-carried chlorhexidine and metronidazole in root canal disinfection
  Yanhong YAN, Peng ZHOU, Haibing LU, Yun GUAN, Ming MA, Juan WANG, Guangwei SHANG, Beizhan JIANG
  Dental Materials Journal.2021; 40(4): 986.     CrossRef
• Evaluation of the Effect of Nitrofurantoin Paste as an Intracanal Medicament on the Chemical Structure of Radicular Dentine
  Mewan Abdulrahman, Bestoon Faraj, Kawa Dizaye
  Sulaimani Dental Journal.2021; 8(2): 8.     CrossRef
• Local Drug Delivery Systems for Vital Pulp Therapy: A New Hope
  Ardavan Parhizkar, Saeed Asgary, Carlo Galli
  International Journal of Biomaterials.2021; 2021: 1.     CrossRef
• Antibiofilm and cytotoxic effect of 3,3′-dihydroxycurcumin (DHC) as photosensitizer agent in antimicrobial photodynamic therapy for endodontic purposes
  Jesse Augusto Pereira, Carlos Roberto Polaquini, VanessaRodrigues dos Santos, Karina Sampaio Caiaffa, Rafaela Laruzo Rabelo, Reinaldo dos Santos Theodoro, Letícia Helena Theodoro, Luis Octavio Regasini, Cristiane Duque
  Photodiagnosis and Photodynamic Therapy.2021; 36: 102534.     CrossRef
• An in-vitro Comparative Evaluation of Quantitative Release of Transforming Growth Factor β-1 from Dentin upon the Action of Endodontic Irrigants, Medicaments, Ultrasonic Activation, and Low-Level Laser Irradiation
  Anilkumar Akhila, V. P. Prabath Singh, Kerala R. Varma, Senthil V. Vasudevan, V. Sukhithasri, Salu Sasikumar
  Amrita Journal of Medicine.2021; 17(2): 34.     CrossRef
• Effect of triple antibiotic paste and calcium hydroxide on the rate of healing of periapical lesions: A systematic review
  NKiran Kumar, Biji Brigit, BS Annapoorna, SavithaB Naik, Seema Merwade, K Rashmi
  Journal of Conservative Dentistry.2021; 24(4): 307.     CrossRef
• Comparison of the efficacy of CanalBrush, EndoActivator, and Passive Ultrasonic Irrigation on the removal of triple antibiotic paste from root canal walls: An in vitro study
  Santosh Kumar, Kavisha Desai, Aparna Palekar, Baswaraj Biradar, Ananjan Chatterjee, Khushboo Kumari
  Journal of International Society of Preventive and Community Dentistry.2020; 10(4): 424.     CrossRef
• Apexification with Calcium Hydroxide vs. Revascularization
  H. Boufdil, M. Mtalsi, S. El Arabi, B. Bousfiha
  Case Reports in Dentistry.2020; 2020: 1.     CrossRef
• Effect of Intracanal Medicaments and Irrigants on the Release of Transforming Growth Factor Beta 1 and Vascular Endothelial Growth Factor from Cervical Root Dentin
  Lívia Nazareth Ferreira, Regina Maria Puppin-Rontani, Fernanda Miori Pascon
  Journal of Endodontics.2020; 46(11): 1616.     CrossRef
• Incorporation of amoxicillin-loaded microspheres in mineral trioxide aggregate cement: an in vitro study
  Fábio Rocha Bohns, Vicente Castelo Branco Leitune, Isadora Martini Garcia, Bruna Genari, Nélio Bairros Dornelles, Silvia Stanisçuaski Guterres, Fabrício Aulo Ogliari, Mary Anne Sampaio de Melo, Fabrício Mezzomo Collares
  Restorative Dentistry & Endodontics.2020;[Epub]     CrossRef
• Assessment of Nitrofurantoin as an Experimental Intracanal Medicament in Endodontics
  Mewan Salahalddin A. Alrahman, Bestoon Muhammed Faraj, Kawa F. Dizaye, Abdelwahab Omri
  BioMed Research International.2020;[Epub]     CrossRef
• An in vitro assessment of effect on microhardness of dentin using vicker's hardness method
  Manoj Chandak, Richa Modi, Rakesh Gogiya, Rakhi Chandak, Anuja Ikhar, Nikhil Mankar
  Journal of Datta Meghe Institute of Medical Sciences University.2020; 15(2): 251.     CrossRef
• Inspection of the Microbiota in Endodontic Lesions
  Mario Dioguardi, Giovanni Di Gioia, Gaetano Illuzzi, Claudia Arena, Vito Carlo Alberto Caponio, Giorgia Apollonia Caloro, Khrystyna Zhurakivska, Iolanda Adipietro, Giuseppe Troiano, Lorenzo Lo Muzio
  Dentistry Journal.2019; 7(2): 47.     CrossRef
• Materials for pulpotomy in immature permanent teeth: a systematic review and meta-analysis
  Yuan Chen, Xinlei Chen, Yali Zhang, Fangjie Zhou, Jiaxin Deng, Jing Zou, Yan Wang
  BMC Oral Health.2019;[Epub]     CrossRef
• Adjunctive antimicrobial photodynamic therapy to conventional chemo-mechanical debridement of infected root canal systems: A systematic review and meta-analysis
  Maryam Pourhajibagher, Abbas bahador
  Photodiagnosis and Photodynamic Therapy.2019; 26: 19.     CrossRef
• Comparison of Antibacterial Effects of Photodynamic Therapy, Modified Triple Antibiotic Paste and Calcium Hydroxide on Root Canals Infected With Enterococcus faecalis: An In Vitro Study
  Mohammad Asnaashari, Mohammad Jafar Eghbal, Amirali Sahba Yaghmayi, Mehdi Shokri, Saranaz Azari-Marhabi
  Journal of Lasers in Medical Sciences.2019; 10(5): S23.     CrossRef