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Morphological evaluation during in vitro chondrogenesis of dental pulp stromal cells
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Choo-Ryung Chung, Ha-Na Kim, Yeul Park, Min-Jeong Kim, Young-Ju Oh, Su-Jung Shin, Yoon-Jeong Choi, Kyung-Ho Kim
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Restor Dent Endod 2012;37(1):34-40. Published online March 2, 2012
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DOI: https://doi.org/10.5395/rde.2012.37.1.34
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 Abstract
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Objectives
The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis.
Materials and Methods
Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets.
Results
Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction.
Conclusions
Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation.
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Citations
Citations to this article as recorded by  - Local myogenic pulp‐derived cell injection enhances craniofacial muscle regeneration in vivo
J. E. Jung, M. J. Song, S. Shin, Y. J. Choi, K. H. Kim, C. J. Chung
Orthodontics & Craniofacial Research.2017; 20(1): 35. CrossRef - Immune Tolerance of Human Dental Pulp-Derived Mesenchymal Stem Cells Mediated by CD4+CD25+ FoxP3+ Regulatory T-Cells and Induced by TGF-β1 and IL-10
Jong Won Hong, Jung Hyun Lim, Chooryung J. Chung, Tae Jo Kang, Tae Yeon Kim, Young Seok Kim, Tae Suk Roh, Dae Hyun Lew
Yonsei Medical Journal.2017; 58(5): 1031. CrossRef - In vitrocharacterization of human dental pulp stem cells isolated by three different methods
Ji-Hyun Jang, Hyeon-Woo Lee, Kyu Min Cho, Hee-Woong Shin, Mo Kwan Kang, Sang Hyuk Park, Euiseong Kim
Restorative Dentistry & Endodontics.2016; 41(4): 283. CrossRef - Effects of two fast-setting calcium-silicate cements on cell viability and angiogenic factor release in human pulp-derived cells
Chooryung J. Chung, Euiseong Kim, Minju Song, Jeong-Won Park, Su-Jung Shin
Odontology.2016; 104(2): 143. CrossRef - A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells andIn VitroChondrogenesis
Subhash C. Juneja, Sowmya Viswanathan, Milan Ganguly, Christian Veillette
Bone Marrow Research.2016; 2016: 1. CrossRef - Local Injection of Pulp Cells Enhances Wound Healing during the Initial Proliferative Phase through the Stimulation of Host Angiogenesis
Hyungjoo Yang, Sujung Shin, Jhiweon Ahn, YoonJeong Choi, Kyung-Ho Kim, Chooryung J. Chung
Journal of Endodontics.2013; 39(6): 788. CrossRef
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