Restorative Dentistry & Endodontics

Fernando Branco Barletta

2 Articles

Reference values for pulp oxygen saturation as a diagnostic tool in endodontics: a systematic review and meta-analysis: Paula Lambert, Sergio Augusto Quevedo Miguens, Caroline Solda, Juliana Tomaz Sganzerla, Leandro Azambuja Reichert, Carlos Estrela, Fernando Branco Barletta; Restor Dent Endod 2020;45(4):e48. Published online October 5, 2020; DOI: https://doi.org/10.5395/rde.2020.45.e48

Objectives

This systematic review aimed to identify mean oxygen saturation values (SpO₂) using pulse oximetry in permanent maxillary anterior teeth.

Materials and Methods

The MEDLINE, Scientific Electronic Library Online, Cochrane Central Register of Controlled Trials, EMBASE, and Literatura Latino Americana em Ciências da Saúde electronic databases were searched. Combinations and variations of “oximetry” AND “dental pulp test” were used as search terms. Studies reporting means and standard deviations of SpO₂ values were included. Two reviewers independently extracted data following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses checklist. Heterogeneity was assessed using the I² statistic, and all analyses were performed using R software. Study quality was assessed using the Quality Assessment of Diagnostic Accuracy Studies-2 tool and the Newcastle-Ottawa scale.

Results

Of the 251 studies identified, 19 met the eligibility criteria and were included (total sample, 4,541 teeth). In the meta-analysis, the mean SpO₂ values were 84.94% (95% confidence interval [CI], 84.85%–85.04%) for the central incisors, 89.29% (95% CI, 89.22%–89.35%) for the lateral incisors, and 89.20% (95% CI, 89.05%–89.34%) for the canines. The studies were predominantly low-quality due to the high risk of bias associated with the index test, unclear risk regarding patient selection, and concerns about outcome assessment.

Conclusions

Although most studies were low-quality, the oxygen saturation levels in normal pulp could be established (minimum saturation, 77.52%). Despite the risk of bias of the included studies, the reference values reported herein are clinically relevant for assessments of changes in pulp status.

Trial Registration

International Prospective Register of Systematic Reviews Identifier: CRD42018085598

Citations

Citations to this article as recorded by

Future trends in endodontics
Foo Suanhow, Tawil Bill
Journal of Applied Biotechnology & Bioengineering.2024; 11(1): 1. CrossRef
Assessment of Pulpal Oxygen Saturation in Caries-free and Carious Maxillary Primary Central Incisors Using a Customized Dental Pulse Oximeter
Shankar Paulindraraj, Hemalatha Ramkumar, Trophimus Gnanabagyan Jayakaran, Kranthi Reddy Kanumuru, Nancy Solomon, Senthil Dakshinamoorthy
International Journal of Clinical Pediatric Dentistry.2023; 16(4): 560. CrossRef
Age-Related Variation of Pulpal Oxygen Saturation in Healthy Primary and Permanent Teeth in Children: A Clinical Study
Andreea Igna, Darian Rusu, Emilia Ogodescu, Ștefania Dinu, Marius Boariu, Adrian Voicu, Ștefan-Ioan Stratul
Journal of Clinical Medicine.2022; 12(1): 170. CrossRef
Pulp oxygen saturation measurement as a diagnostic tool for assessing pulp status in primary teeth
Kanamarlapudi Venkata Saikiran, Deepa Gurunathan, Sainath Reddy Elicherla, Sreekanth Kumar Mallineni, Sivakumar Nuvvula
Journal of Indian Society of Pedodontics and Preventive Dentistry.2022; 40(4): 349. CrossRef
Diagnostic Value of Serum Chitinase‐3‐Like Protein 1 for Liver Fibrosis: A Meta‐analysis
Xiaoting Huang, Jialing Zhuang, Yongqiang Yang, Jiaxin Jian, Wen Ai, Chunyong Liu, Wenzhi Tang, Changyu Jiang, Yongshen He, Lesheng Huang, Se Peng, Jin Shui Pan
BioMed Research International.2022;[Epub] CrossRef
Assessment of Pulpal Status in Primary Teeth Following Direct Pulp Capping in an Experimental Canine Model
Andreea Igna, Cornel Igna, Mariana Ioana Miron, Larisa Schuszler, Roxana Dascălu, Mihaela Moldovan, Adrian Aristide Voicu, Carmen Darinca Todea, Marius Boariu, Maria-Alexandra Mârțu, Ștefan-Ioan Stratul
Diagnostics.2022; 12(8): 2022. CrossRef

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Cytocompatibility and cell proliferation evaluation of calcium phosphate-based root canal sealers: Letícia Boldrin Mestieri, Ivana Maria Zaccara, Lucas Siqueira Pinheiro, Fernando Branco Barletta, Patrícia Maria Polli Kopper, Fabiana Soares Grecca; Restor Dent Endod 2020;45(1):e2. Published online November 15, 2019; DOI: https://doi.org/10.5395/rde.2020.45.e2

Abstract PDF PubReader ePub

Objectives

This study aimed to evaluate the cell viability and migration of Endosequence Bioceramic Root Canal Sealer (BC Sealer) compared to MTA Fillapex and AH Plus.

Materials and Methods

BC Sealer, MTA Fillapex, and AH Plus were placed in contact with culture medium to obtain sealers extracts in dilution 1:1, 1:2 and 1:4. 3T3 cells were plated and exposed to the extracts. Cell viability and migration were assessed by 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide (MTT) and Scratch assay, respectively. Data were analyzed by Kruskal-Wallis and Dunn's test (p < 0.05).

Results

The MTT assay revealed greater cytotoxicity for AH Plus and MTA Fillapex at 1:1 dilution when compared to control (p < 0.05). At 1:2 and 1:4 dilutions, all sealers were similar to control (p > 0.05) and MTA Fillapex was more cytotoxic than BC Sealer (p < 0.05). Scratch assay demonstrated the continuous closure of the wound according to time. At 30 hours, the control group presented closure of the wound (p < 0.05). At 36 hours, only BC Sealer presented the closure when compared to AH Plus and MTA Fillapex (p < 0.05). At 42 hours, AH Plus and MTA Fillapex showed a wound healing (p > 0.05).

Conclusions

All tested sealers demonstrated cell viability highlighting BC Sealer, which showed increased cell migration capacity suggesting that this sealer may achieve better tissue repair when compared to other tested sealers.

Citations

Citations to this article as recorded by

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Journal of Conservative Dentistry and Endodontics.2024; 27(4): 345. CrossRef
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Azam haddadikohsar, Mohammad shokrzade, Marjan Fallah, Fatemeh Shakeri
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Guanglei Zhou, Yu Zhao, Liangjing Cai, Liwei Liu, Xu Li, Lu Sun, Jiayin Deng
BMC Oral Health.2024;[Epub] CrossRef
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Yujia Yan, Yanyao Li, Yaqi Chi, Mengzhen Ji, Ya Shen, Ling Zou
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Cytotoxicity and Genotoxicity of Epoxy Resin-Based Root Canal Sealers before and after Setting Procedures
Mijoo Kim, Marc Hayashi, Bo Yu, Thomas K. Lee, Reuben H. Kim, Deuk-won Jo
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Characterization, Antimicrobial Effects, and Cytocompatibility of a Root Canal Sealer Produced by Pozzolan Reaction between Calcium Hydroxide and Silica
Mi-Ah Kim, Vinicius Rosa, Prasanna Neelakantan, Yun-Chan Hwang, Kyung-San Min
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Bone repair in defects filled with AH Plus sealer and different concentrations of MTA: a study in rat tibiae
Jessica Emanuella Rocha Paz, Priscila Oliveira Costa, Albert Alexandre Costa Souza, Ingrid Macedo de Oliveira, Lucas Fernandes Falcão, Carlos Alberto Monteiro Falcão, Maria Ângela Area Leão Ferraz, Lucielma Salmito Soares Pinto
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Incorporation of amoxicillin-loaded microspheres in mineral trioxide aggregate cement: an in vitro study
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