Restorative Dentistry & Endodontics

Angelo Rafael de Vito Bordin

1 Article

Analysis of the shelf life of chitosan stored in different types of packaging, using colorimetry and dentin microhardness: Antonio Miranda da Cruz-Filho, Angelo Rafael de Vito Bordin, Luis Eduardo Souza-Flamini, Débora Fernandes da Costa Guedes, Paulo César Saquy, Ricardo Gariba Silva, Jesus Djalma Pécora; Restor Dent Endod 2017;42(2):87-94. Published online March 27, 2017; DOI: https://doi.org/10.5395/rde.2017.42.2.87

Objectives

Chitosan has been widely investigated and used. However, the literature does not refer to the shelf life of this solution. This study evaluated, through the colorimetric titration technique and an analysis of dentin micro-hardness, the shelf life of 0.2% chitosan solution.

Materials and Methods

Thirty human canines were sectioned, and specimens were obtained from the second and third slices, from cemento-enamel junction to the apex. A 0.2% chitosan solution was prepared and distributed in 3 identical glass bottles (v1, v2, and v3) and 3 plastic bottles (p1, p2, and p3). At 0, 7, 15, 30, 45, 60, 90, 120, 150, and 180 days, the specimens were immersed in each solution for 5 minutes (n = 3 each). The chelating effect of the solution was assessed by micro-hardness and colorimetric analysis of the dentin specimens. 17% EDTA and distilled water were used as controls. Data were analyzed statistically by two-way and Tukey-Kramer multiple comparison (α = 0.05).

Results

There was no statistically significant difference among the solutions with respect to the study time (p = 0.113) and micro-hardness/time interaction (p = 0.329). Chitosan solutions and EDTA reduced the micro-hardness in a similar manner and differed significantly from the control group (p < 0.001). Chitosan solutions chelated calcium ions throughout the entire experiment.

Conclusions

Regardless of the storage form, chitosan demonstrates a chelating property for a minimum period of 6 months.

Citations

Citations to this article as recorded by

Chitosan’s Ability to Remove the Smear Layer—A Systematic Review of Ex Vivo Studies
Ana Ferreira-Reguera, Inês Ferreira, Irene Pina-Vaz, Benjamín Martín-Biedma, José Martín-Cruces
Medicina.2025; 61(1): 114. CrossRef
Antimicrobial evaluation of root canal irrigants of natural sources with and without gamma radiation activation - An in vitro study
Hoda Raafat Yousri, Abeer Hashem Mahran, Ahmed Abdel Rahman Hashem, Amal A. El-Batouti
Endodontology.2024; 36(4): 383. CrossRef
Influence of Chitosan 0.2% in Various Final Cleaning Methods on the Bond Strength of Fiberglass Post to Intrarradicular Dentin
Naira Geovana Camilo, Alex da Rocha Gonçalves, Larissa Pinzan Flauzino, Cristiane Martins Rodrigues Bernardes, Andreza Maria Fábio Aranha, Priscilla Cardoso Lazari-Carvalho, Marco Aurélio de Carvalho, Helder Fernandes de Oliveira
Polymers.2023; 15(22): 4409. CrossRef
Evaluation and comparison of anti-inflammatory properties of ibuprofen using two drug delivery systems after third molar surgery: using chitosan microspheres as a carrier for local drug delivery in to the third molar socket and through the oral route
Karthik KP, Balamurugan R
British Journal of Oral and Maxillofacial Surgery.2021; 59(2): 191. CrossRef
Optimization of chitosan nanoparticle synthesis and its potential application as germination elicitor of Oryza sativa L.
K. Divya, Smitha Vijayan, Sreekumar Janardanan Nair, M.S. Jisha
International Journal of Biological Macromolecules.2019; 124: 1053. CrossRef
Crosstalk between chitosan and cell signaling pathways
Behrouz Farhadihosseinabadi, Amir Zarebkohan, Mohamad Eftekhary, Mohammad Heiat, Mehrdad Moosazadeh Moghaddam, Mazaher Gholipourmalekabadi
Cellular and Molecular Life Sciences.2019; 76(14): 2697. CrossRef

186 View
2 Download
6 Crossref

Author index